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The map label for this gene is ntcA [H]

Identifier: 148379314

GI number: 148379314

Start: 1459149

End: 1459871

Strand: Reverse

Name: ntcA [H]

Synonym: CBO1335

Alternate gene names: 148379314

Gene position: 1459871-1459149 (Counterclockwise)

Preceding gene: 148379319

Following gene: 148379313

Centisome position: 37.56

GC content: 27.8

Gene sequence:

>723_bases
TTGATTTATAAAATTTCATTAAAGAAAGGATTTGGCATGGTAAAAAAGAATAGATTTCTTCCTGGTTCTTCTATAGATAT
TAATTCTGATTTTTATAAAATATTTGAAGAAGCTGGTACAATTAAAAAATACAGCAAAGATGAAATAATCTATTTCCAAG
AGGATGTGGCAGAAAACTTTTATTTAATAAAATCTGGTCGTGTTAGAATGTTTCTTATCTCGCCTGAAGGAACTGAGCTT
ACTATTGAAATATTAAGAAAAGGAAAATTATTTGGAGAATCTTCCTGCTTTAGTTATGGATCTAGATTAACATCTGTTAG
CGCTGCTACAGATGTCGAATTAATTTCAGTAAGTCTGGAAAATTTGTACCCCTATCTAACTAAGTATCCAGAATTAATGG
TTCAAATGTTTCATCTTATGTCCTTAACAATGAAAAATCTATCGATTCAAGTTAGTAATATGGCCTTTTTACCAGCAGAA
AAAAAACTTGCCCAATTACTAGTAAGATTAGGCGCGCATTTTAAAAAAAATAAAAATGATAAATATTATATTATTGATTA
TTCTCACGAAGAAATTGCTCAACTTATTGGTAGCTGTAGAGTTACAGTTACTAAGATATTAAATTCTTTTCAAGAAAAAG
GAATGATTTCCTTAGGATATAAGAAAATTAAAATTATAGACGAAAAAGGATTAAAAAAAGAATACTACGACTATTTTGTA
TAA

Upstream 100 bases:

>100_bases
AGTTTTTCTTTAGTCTCCATAATATTTCTGCCTCCCATAAAAATTCTGCAAATTAAATTTTACTCTTAACCTTGAAAATA
GATTGTAGTTTAGGCTACAA

Downstream 100 bases:

>100_bases
ACTTATATGTAAAAAATTTAAAATCTTTGATAAAACATTACCATAACATAGATAAAGTGGTATAATTGTATTGGCGTACA
TAAGGTAGGCTTATTTCTTT

Product: Crp/Fnr family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MIYKISLKKGFGMVKKNRFLPGSSIDINSDFYKIFEEAGTIKKYSKDEIIYFQEDVAENFYLIKSGRVRMFLISPEGTEL
TIEILRKGKLFGESSCFSYGSRLTSVSAATDVELISVSLENLYPYLTKYPELMVQMFHLMSLTMKNLSIQVSNMAFLPAE
KKLAQLLVRLGAHFKKNKNDKYYIIDYSHEEIAQLIGSCRVTVTKILNSFQEKGMISLGYKKIKIIDEKGLKKEYYDYFV

Sequences:

>Translated_240_residues
MIYKISLKKGFGMVKKNRFLPGSSIDINSDFYKIFEEAGTIKKYSKDEIIYFQEDVAENFYLIKSGRVRMFLISPEGTEL
TIEILRKGKLFGESSCFSYGSRLTSVSAATDVELISVSLENLYPYLTKYPELMVQMFHLMSLTMKNLSIQVSNMAFLPAE
KKLAQLLVRLGAHFKKNKNDKYYIIDYSHEEIAQLIGSCRVTVTKILNSFQEKGMISLGYKKIKIIDEKGLKKEYYDYFV
>Mature_240_residues
MIYKISLKKGFGMVKKNRFLPGSSIDINSDFYKIFEEAGTIKKYSKDEIIYFQEDVAENFYLIKSGRVRMFLISPEGTEL
TIEILRKGKLFGESSCFSYGSRLTSVSAATDVELISVSLENLYPYLTKYPELMVQMFHLMSLTMKNLSIQVSNMAFLPAE
KKLAQLLVRLGAHFKKNKNDKYYIIDYSHEEIAQLIGSCRVTVTKILNSFQEKGMISLGYKKIKIIDEKGLKKEYYDYFV

Specific function: Required for full expression of proteins subject to ammonium repression. Transcriptional activator of genes subject to nitrogen control [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=186, Percent_Identity=27.4193548387097, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR022299
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 27697; Mature: 27697

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYKISLKKGFGMVKKNRFLPGSSIDINSDFYKIFEEAGTIKKYSKDEIIYFQEDVAENF
CEEEEEECCCCCCHHCCCCCCCCCEECCHHHHHHHHHCCCHHCCCCCCEEEEECHHHCCE
YLIKSGRVRMFLISPEGTELTIEILRKGKLFGESSCFSYGSRLTSVSAATDVELISVSLE
EEEECCCEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHEEEEHH
NLYPYLTKYPELMVQMFHLMSLTMKNLSIQVSNMAFLPAEKKLAQLLVRLGAHFKKNKND
HHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECEEECCCHHHHHHHHHHHHHHHCCCCCC
KYYIIDYSHEEIAQLIGSCRVTVTKILNSFQEKGMISLGYKKIKIIDEKGLKKEYYDYFV
CEEEEEECHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCEEEEEECCCCCCHHHHHHCC
>Mature Secondary Structure
MIYKISLKKGFGMVKKNRFLPGSSIDINSDFYKIFEEAGTIKKYSKDEIIYFQEDVAENF
CEEEEEECCCCCCHHCCCCCCCCCEECCHHHHHHHHHCCCHHCCCCCCEEEEECHHHCCE
YLIKSGRVRMFLISPEGTELTIEILRKGKLFGESSCFSYGSRLTSVSAATDVELISVSLE
EEEECCCEEEEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHEEEEHH
NLYPYLTKYPELMVQMFHLMSLTMKNLSIQVSNMAFLPAEKKLAQLLVRLGAHFKKNKND
HHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECEEECCCHHHHHHHHHHHHHHHCCCCCC
KYYIIDYSHEEIAQLIGSCRVTVTKILNSFQEKGMISLGYKKIKIIDEKGLKKEYYDYFV
CEEEEEECHHHHHHHHHCCHHHHHHHHHHHHHCCCEEECCEEEEEECCCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8366058; 8905231 [H]