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The map label for this gene is nasD [H]

Identifier: 148379312

GI number: 148379312

Start: 1456519

End: 1457745

Strand: Direct

Name: nasD [H]

Synonym: CBO1333

Alternate gene names: 148379312

Gene position: 1456519-1457745 (Clockwise)

Preceding gene: 148379311

Following gene: 148379315

Centisome position: 37.47

GC content: 29.26

Gene sequence:

>1227_bases
ATGAAATATGTGATAATAGGGGCTAGTGCAGCAGGAATTTCAGCAGTTAAAACTTTGAGAACCTTAGATAAAGATTCTCA
AATAGTTATGATTTCTAAAGATGATAAAGTATATTCAAGATGTTTGTTGCATCATTTTATAGGTGGAAATAGAGAGATAG
AAGGATTATCTTTTATAGAAAAGGATTTTTTCGCTAAATATAATGTAAAATGGATAAAAAATAAAAAAGTAGAAACTGTA
GATATAGATAAAAAGGTAGTAAAAGTGCAAGGAAAAATTTCAGAACATTATGATAAATTGCTCATAGCATCAGGTTCTTA
TGCGTCTATACCGCCGATTAAAAATTTAAGAGAAGCTAAAAGAGTATACACTTTAAGGAATTTAGATGATGCAATAGCTA
TAAAAGAAGAGGCTAAAAAAATTAAAAAAGCAGCAGTAATAGGGGCAGGACTTGTTGGGATAGATGCTACTATGGGATTA
TTAGAAAACGACGTAGAGGTAACTGTTATAGAAATGGGAAATAGAATGCTTCCACTGCAATTAGACCAAAGAGCATCAAA
AACTTACGAAGAATTATTTAGAAAACACAATGTTAATATAAAAACCAGTGTAGGAGCTGAAGAAGCTTTAATAGATGGGA
ATAGAAATGTTTGTGGAATAAAATTAAGTAATGGTGAAAAGATAGATTGTGATATTATAATTGTAGCAGCAGGAGTTAGA
CCTAATATAGATTTTATAAAGGATGAAAGAATAAAGATAGAAAGAGGCATAGTTATAAATGATAATTGTGAAACTACAGT
TAAAGATATATATGCTGCAGGAGACGTAACCTTTAGAGCACCTATATGGCCAATAGCTATGAAACAAGGAAGAATAGCTG
CTTGTAATATGGCAGGAGAAAAGAAGCTATTAGATGATAATTTCGGAGCAAGAAATTCTATGAATTTTCTAGGTGTATAT
ACTATATCTTTAGGAATAGTGGAACCACAGGATGAAAGTTATAAAGTAGATATAATAGATAAAAATGGAGTTTATAAAAA
AATAATACACAAAGATGGAATCATATATGGAGCCATACTTCAGGGAGATATAGCTTATGCAGGAGTATTAACAGAGCTTA
TAAAAAATAAAATAGATATATCTAAAATAGATAAGGATATATTTGATATAAGTTTTGCAGACTTTTTTAATATAAAAGAA
AATGGAGAATTTAGTTACAAAAAATAA

Upstream 100 bases:

>100_bases
GGATGCAAATAAATTAGCAGAGATTAGTATAAAATTTTCTTTAATAAGAATTTGATTTTATGATATTATAAAAAATATTA
AGGCTCGGGAGGTAAATAAT

Downstream 100 bases:

>100_bases
GGAAAGTGCGAGGCACTTTCCTTAAAATATTTTATTTAATCTCAGTTTTCATAGGTGGTTTCAGAGATTATTTCTAAGTT
TTTATTAGGTTTTTTGAATA

Product: nitrate reductase, NADH oxidase subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MKYVIIGASAAGISAVKTLRTLDKDSQIVMISKDDKVYSRCLLHHFIGGNREIEGLSFIEKDFFAKYNVKWIKNKKVETV
DIDKKVVKVQGKISEHYDKLLIASGSYASIPPIKNLREAKRVYTLRNLDDAIAIKEEAKKIKKAAVIGAGLVGIDATMGL
LENDVEVTVIEMGNRMLPLQLDQRASKTYEELFRKHNVNIKTSVGAEEALIDGNRNVCGIKLSNGEKIDCDIIIVAAGVR
PNIDFIKDERIKIERGIVINDNCETTVKDIYAAGDVTFRAPIWPIAMKQGRIAACNMAGEKKLLDDNFGARNSMNFLGVY
TISLGIVEPQDESYKVDIIDKNGVYKKIIHKDGIIYGAILQGDIAYAGVLTELIKNKIDISKIDKDIFDISFADFFNIKE
NGEFSYKK

Sequences:

>Translated_408_residues
MKYVIIGASAAGISAVKTLRTLDKDSQIVMISKDDKVYSRCLLHHFIGGNREIEGLSFIEKDFFAKYNVKWIKNKKVETV
DIDKKVVKVQGKISEHYDKLLIASGSYASIPPIKNLREAKRVYTLRNLDDAIAIKEEAKKIKKAAVIGAGLVGIDATMGL
LENDVEVTVIEMGNRMLPLQLDQRASKTYEELFRKHNVNIKTSVGAEEALIDGNRNVCGIKLSNGEKIDCDIIIVAAGVR
PNIDFIKDERIKIERGIVINDNCETTVKDIYAAGDVTFRAPIWPIAMKQGRIAACNMAGEKKLLDDNFGARNSMNFLGVY
TISLGIVEPQDESYKVDIIDKNGVYKKIIHKDGIIYGAILQGDIAYAGVLTELIKNKIDISKIDKDIFDISFADFFNIKE
NGEFSYKK
>Mature_408_residues
MKYVIIGASAAGISAVKTLRTLDKDSQIVMISKDDKVYSRCLLHHFIGGNREIEGLSFIEKDFFAKYNVKWIKNKKVETV
DIDKKVVKVQGKISEHYDKLLIASGSYASIPPIKNLREAKRVYTLRNLDDAIAIKEEAKKIKKAAVIGAGLVGIDATMGL
LENDVEVTVIEMGNRMLPLQLDQRASKTYEELFRKHNVNIKTSVGAEEALIDGNRNVCGIKLSNGEKIDCDIIIVAAGVR
PNIDFIKDERIKIERGIVINDNCETTVKDIYAAGDVTFRAPIWPIAMKQGRIAACNMAGEKKLLDDNFGARNSMNFLGVY
TISLGIVEPQDESYKVDIIDKNGVYKKIIHKDGIIYGAILQGDIAYAGVLTELIKNKIDISKIDKDIFDISFADFFNIKE
NGEFSYKK

Specific function: Required for nitrite assimilation [H]

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=313, Percent_Identity=28.4345047923323, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI65787454, Length=313, Percent_Identity=28.4345047923323, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI226437568, Length=313, Percent_Identity=28.4345047923323, Blast_Score=106, Evalue=3e-23,
Organism=Escherichia coli, GI1789765, Length=404, Percent_Identity=26.980198019802, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789065, Length=303, Percent_Identity=25.7425742574257, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1788892, Length=219, Percent_Identity=25.5707762557078, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI32564386, Length=425, Percent_Identity=25.1764705882353, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17559934, Length=294, Percent_Identity=26.530612244898, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24639257, Length=352, Percent_Identity=25, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI281359715, Length=352, Percent_Identity=25, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI281359713, Length=352, Percent_Identity=25, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24639250, Length=352, Percent_Identity=25, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI18543267, Length=352, Percent_Identity=25, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24639252, Length=352, Percent_Identity=25, Blast_Score=109, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24585130, Length=312, Percent_Identity=24.3589743589744, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585384, Length=194, Percent_Identity=28.8659793814433, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 45491; Mature: 45491

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYVIIGASAAGISAVKTLRTLDKDSQIVMISKDDKVYSRCLLHHFIGGNREIEGLSFIE
CEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHH
KDFFAKYNVKWIKNKKVETVDIDKKVVKVQGKISEHYDKLLIASGSYASIPPIKNLREAK
HHHHHCCCEEEECCCCEEEEECCCEEEEEECCHHHCCCEEEEECCCCCCCCCHHHHHHHH
RVYTLRNLDDAIAIKEEAKKIKKAAVIGAGLVGIDATMGLLENDVEVTVIEMGNRMLPLQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCEEEEEECCCEEECEE
LDQRASKTYEELFRKHNVNIKTSVGAEEALIDGNRNVCGIKLSNGEKIDCDIIIVAAGVR
CCHHHHHHHHHHHHHCCCCEEECCCCCCEEECCCCCEEEEEECCCCEEEEEEEEEEECCC
PNIDFIKDERIKIERGIVINDNCETTVKDIYAAGDVTFRAPIWPIAMKQGRIAACNMAGE
CCCCEECCCCEEEECCEEECCCCCHHHHHHHCCCCEEEECCCCEEEECCCCEEEECCCCC
KKLLDDNFGARNSMNFLGVYTISLGIVEPQDESYKVDIIDKNGVYKKIIHKDGIIYGAIL
CCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCEEEEEEE
QGDIAYAGVLTELIKNKIDISKIDKDIFDISFADFFNIKENGEFSYKK
ECCHHHHHHHHHHHHCCCCHHHHCHHHHEEEHHHEECCCCCCCCCCCC
>Mature Secondary Structure
MKYVIIGASAAGISAVKTLRTLDKDSQIVMISKDDKVYSRCLLHHFIGGNREIEGLSFIE
CEEEEEECCCCCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCHHHHH
KDFFAKYNVKWIKNKKVETVDIDKKVVKVQGKISEHYDKLLIASGSYASIPPIKNLREAK
HHHHHCCCEEEECCCCEEEEECCCEEEEEECCHHHCCCEEEEECCCCCCCCCHHHHHHHH
RVYTLRNLDDAIAIKEEAKKIKKAAVIGAGLVGIDATMGLLENDVEVTVIEMGNRMLPLQ
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCEEEEEECCCEEECEE
LDQRASKTYEELFRKHNVNIKTSVGAEEALIDGNRNVCGIKLSNGEKIDCDIIIVAAGVR
CCHHHHHHHHHHHHHCCCCEEECCCCCCEEECCCCCEEEEEECCCCEEEEEEEEEEECCC
PNIDFIKDERIKIERGIVINDNCETTVKDIYAAGDVTFRAPIWPIAMKQGRIAACNMAGE
CCCCEECCCCEEEECCEEECCCCCHHHHHHHCCCCEEEECCCCEEEECCCCEEEECCCCC
KKLLDDNFGARNSMNFLGVYTISLGIVEPQDESYKVDIIDKNGVYKKIIHKDGIIYGAIL
CCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCEEEEEEE
QGDIAYAGVLTELIKNKIDISKIDKDIFDISFADFFNIKENGEFSYKK
ECCHHHHHHHHHHHHCCCCHHHHCHHHHEEEHHHEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]