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The map label for this gene is sleB [H]

Identifier: 148379303

GI number: 148379303

Start: 1444855

End: 1445634

Strand: Reverse

Name: sleB [H]

Synonym: CBO1324

Alternate gene names: 148379303

Gene position: 1445634-1444855 (Counterclockwise)

Preceding gene: 148379306

Following gene: 148379302

Centisome position: 37.19

GC content: 32.69

Gene sequence:

>780_bases
ATGACAAAGCACAAATCATTAAAAACACTCTTAGCTACACTAACTTTTTGTGTAGGTTTATCTAGTCCAGTCTTTGCTGC
AGATTACAGGGTAAGTTCTGGTGATTCTCTTTACAAGATAGGTCAATTATTCAATGTATCTTCAGACACTATTATAAAAA
ACAATAATCTTAAGGAAAGCACAATTCATCCTGGACAAGTTCTAAACATTCCTTGCAATACTTATACTGTAAAAAGTGGA
GATAGTCTATTTTTAATTTCTAAAGCGCAGGGCATAAGTTTATACAATTTAAGAAAAGCAAATAATAAATTGGATGACAC
AATTTATCCTGGACAAGTTTTGAATTTACCTGGAAAAACTTCTAGTAATACTAGTCCAGCACCAGCTCCAACTACACCAA
AGCCTATAGTGAATTATACAGAATCAGATTTAGACCTTTTAGCTAGACTTATAACAGCTGAAGCTCAAAGTGAACCCTAT
AGCGCTCAAGTAGCTGTGGCGTCTGTAGTTATAAACAGAATAAAAAGTTCTCAATTTCCAAACAGTATTTCATCTGTAAT
TTATCAAAAAAGTGATGGATACTATCAATTTACACCTGTATTAAATGGTTGGATAAATAAACCTGCTACAGAAACATCAA
AAAAAGCTGCTAAAGAAGCTTTATATGGATCTGACCCAAGCAAAGGTGCACTATATTATTTTGATAATACTGCCACTAAT
AAATGGTTATGGTCCAAGCCTATAACAGCTAGAATTGGTAACATGGTTTATGTTAAATAA

Upstream 100 bases:

>100_bases
AAGTACCGGGGATACTCTTGGGGTGAATCTCTTAGGAATAGGGTACCTCTAACCCGAACCCGTCAACTAACCTGGGAGGC
AATATGGAGGGATTTAACTA

Downstream 100 bases:

>100_bases
TAATATTATTATTTAAGGGCCACTATCAAGATTTAAAATATTGTTATAAATAACAAACCAACAATAAATAAAAATTTTTA
ATAAACAACATACTTAAAAT

Product: putative cell wall hydrolase

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: SCLE; Germination-specific amidase [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTKHKSLKTLLATLTFCVGLSSPVFAADYRVSSGDSLYKIGQLFNVSSDTIIKNNNLKESTIHPGQVLNIPCNTYTVKSG
DSLFLISKAQGISLYNLRKANNKLDDTIYPGQVLNLPGKTSSNTSPAPAPTTPKPIVNYTESDLDLLARLITAEAQSEPY
SAQVAVASVVINRIKSSQFPNSISSVIYQKSDGYYQFTPVLNGWINKPATETSKKAAKEALYGSDPSKGALYYFDNTATN
KWLWSKPITARIGNMVYVK

Sequences:

>Translated_259_residues
MTKHKSLKTLLATLTFCVGLSSPVFAADYRVSSGDSLYKIGQLFNVSSDTIIKNNNLKESTIHPGQVLNIPCNTYTVKSG
DSLFLISKAQGISLYNLRKANNKLDDTIYPGQVLNLPGKTSSNTSPAPAPTTPKPIVNYTESDLDLLARLITAEAQSEPY
SAQVAVASVVINRIKSSQFPNSISSVIYQKSDGYYQFTPVLNGWINKPATETSKKAAKEALYGSDPSKGALYYFDNTATN
KWLWSKPITARIGNMVYVK
>Mature_258_residues
TKHKSLKTLLATLTFCVGLSSPVFAADYRVSSGDSLYKIGQLFNVSSDTIIKNNNLKESTIHPGQVLNIPCNTYTVKSGD
SLFLISKAQGISLYNLRKANNKLDDTIYPGQVLNLPGKTSSNTSPAPAPTTPKPIVNYTESDLDLLARLITAEAQSEPYS
AQVAVASVVINRIKSSQFPNSISSVIYQKSDGYYQFTPVLNGWINKPATETSKKAAKEALYGSDPSKGALYYFDNTATNK
WLWSKPITARIGNMVYVK

Specific function: Probable N-acetylmuramyl-L-alanine amidase. Required for spore cortex hydrolysis during germination. May form a complex with some hydrophobic spore component, leading to a stabilization of the enzyme in a spore-bound form [H]

COG id: COG3773

COG function: function code M; Cell wall hydrolyses involved in spore germination

Gene ontology:

Cell location: Forespore. Note=Expressed in the forespore and then transported across the inner forespore membrane and deposited on the outside of the cortex [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sleB family [H]

Homologues:

None

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011105
- InterPro:   IPR002477
- InterPro:   IPR014224 [H]

Pfam domain/function: PF07486 Hydrolase_2; PF01471 PG_binding_1 [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 28275; Mature: 28144

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKHKSLKTLLATLTFCVGLSSPVFAADYRVSSGDSLYKIGQLFNVSSDTIIKNNNLKES
CCCHHHHHHHHHHHHHHHCCCCCCEEECEEECCCCCHHHHHHHHCCCCCCEEECCCCCCC
TIHPGQVLNIPCNTYTVKSGDSLFLISKAQGISLYNLRKANNKLDDTIYPGQVLNLPGKT
CCCCCCEEEECCCEEEECCCCEEEEEECCCCCEEEEEHHCCCCCCCCCCCCEEEECCCCC
SSNTSPAPAPTTPKPIVNYTESDLDLLARLITAEAQSEPYSAQVAVASVVINRIKSSQFP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
NSISSVIYQKSDGYYQFTPVLNGWINKPATETSKKAAKEALYGSDPSKGALYYFDNTATN
HHHHHHHEECCCCCEEEECHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
KWLWSKPITARIGNMVYVK
CEEECCCHHHHHCCEEEEC
>Mature Secondary Structure 
TKHKSLKTLLATLTFCVGLSSPVFAADYRVSSGDSLYKIGQLFNVSSDTIIKNNNLKES
CCHHHHHHHHHHHHHHHCCCCCCEEECEEECCCCCHHHHHHHHCCCCCCEEECCCCCCC
TIHPGQVLNIPCNTYTVKSGDSLFLISKAQGISLYNLRKANNKLDDTIYPGQVLNLPGKT
CCCCCCEEEECCCEEEECCCCEEEEEECCCCCEEEEEHHCCCCCCCCCCCCEEEECCCCC
SSNTSPAPAPTTPKPIVNYTESDLDLLARLITAEAQSEPYSAQVAVASVVINRIKSSQFP
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
NSISSVIYQKSDGYYQFTPVLNGWINKPATETSKKAAKEALYGSDPSKGALYYFDNTATN
HHHHHHHEECCCCCEEEECHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECCCCC
KWLWSKPITARIGNMVYVK
CEEECCCHHHHHCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8752358; 8081503; 10197998 [H]