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The map label for this gene is pcrA [H]

Identifier: 148379300

GI number: 148379300

Start: 1439124

End: 1441691

Strand: Direct

Name: pcrA [H]

Synonym: CBO1321

Alternate gene names: 148379300

Gene position: 1439124-1441691 (Clockwise)

Preceding gene: 148379299

Following gene: 148379304

Centisome position: 37.02

GC content: 24.53

Gene sequence:

>2568_bases
ATGTTAGATATATATCTTAATGATAAACAGAAAAAAGTAATAAATACATTAGATAAAAATATACTCCTTTTGTCTTCTGC
AGGTACAGGGAAAACAAAAACATTAAGTATGAGGATAGGAAATATAATAGCTAAAAACTTAGCTTTAGGAGAGCAAATAC
TTTGTTTAACTTTTACTAATAGAGCCTGTAAGGAAATGAAAGAAAAAATAATAGAAACAGTAGGGAAAGAGGGATTAAAA
GTTACTGTAAAGACTTTTCATAGTTTTTGTTTTGATGTAATAAAAAAAGAGGCAAAAAAGAATACAGATATTTCCTCTGA
TTTTACTATATATGATGAAGAAGATACAAAGGAAATAATAAGTGAATTAAATTCATATAAGTTTAATGTTTCTTGTCTTC
AAAGATTTATAAACATTGTTAAAGAAAAAAGTATAAATTATAAAGATAGAGATTATAAAGATGTTATAGATACTATGATA
AAATATAATTCCAACATATTTAATGATATATGTAAAAATGAAAAATATATGGTAGATAAAACTTTGGTAGATTATTTAAA
AATATATGGATATTTAATGGTGATAAGCTATGATAAAAAGCTATATGAAAGGCATGGATTAGATTTTAATGATTTAATAA
TAAAGGTTTATGACTTATTTCAAAAGGATAAAATAACTACATATTGGAGAGAAAAATATAAATTTATTCATATAGATGAA
ATGCAGGATACCAGTGAAATAGAATATAATATAGTTTCAAAATTATTTAGAAATAACAATATAATGCTTAGTGGCGATTA
TTACCAGACTATATATGAATGGAGAGGATCTAATCCTATCATAATATTTGATAAGTATAAAAAAGAATATAATCCGGAAT
TTATAGTTTTTGATAAGAACTATAGATCTTCAGAAATATTATTAAAGGCCTCATATGAATTTTTACTAAATGCTTTTGGA
AAAGATGTAAATGAAATATATAAAGAAGGTATAGAGCCTAAAGCTAATATAAAAGGGGATCTAATAGAATTTAAGAAAGC
TTATGATTTTATGGAAGAGGCAACTTTTATATTTAATAAAATAAAATCTTTAAAGGTAGAAAAGCTTTCAAGAGTTGCTA
TATTAACTAGGAATAATAGAATAAATATAGCTTTAAGTGAAAAGTTTAAAATACTAAATTCAAAGCTTCCAAAAGAAGAG
AGAGTGGGATTCCTTTTGGTGGATGAATTTAAATTCTTTAGAAGAGAGGAAATAAAGGATGTATTAGCTTATTTAAAACT
TATAGTAAATGATTTTGATAGTAATAGTTTAAAAAGGATATTAAAGAAGTTTGGAAAGGGCATAGGAGAAAGAACCATAG
AAGAAATAGAAAAAGAGGAAAATATAAAAATAGGTGTAAGACTTACAGATTTAATTCATAAGAATACTCAAGAATATGGA
GATTATTATGAGCTTTTAACTAGAGGATTAGATAATGAAAATGTAGTAGTTTTTGATGTAGAAAGCACAGGGATTAATAC
TACAGAGGATGAAATAGTGCAAATTGCAGGTATTAAAATAAACAATAAAGGAGAAGTTATAGAAAGTTTTCAAAGATTTT
TGACTCCCAAGAAGTTTGTAGGGGACTCTTATTTAGTTCATGGTTTTAGTGACCAATTTTTAAAAGAAAATGGAGAAAAT
AAAAAGGTAGTTTTAAAAGATTTTTTAGAATTTATAAAAGATACAGTAATAGTTGGACATAATGTAGCCTTTGATATATC
CATATTAAATAGTGAACTTGAAAGATTAAGTTTAGAGAAAGCAAGTTTTAAAACCTATTATGATACTTTAGATATAGCTA
GAAGATTTTATCCTAACTTAACAAATCATAAATTAGAAACTTTAAGTAAGTTATTTAATACAGAAACGAAATCTAGCCAT
GATGCTATGGATGATATATTGGCTACAAAAGATGTTCTTATGGCTATGCTAAAGGAAAAAGTTAAACCTACTATAATAAA
TAGAATGGTAGTGTACGGAAAATATATTAAAAAATTTCAACCTATATATAAAGAAATAACTAATTTAAAAAAATTAACTT
ATATAAAAAATCCGAAGGACATAATAGGAGAAATAGTTAATAAAACGAAAATAAAGGAAATATACAAAGACCAATATATT
AAGATAAACAATTTGAGGGAATTTTTTGTTATAGCTAAAGAAATAGAAAATAAAGAAATTTCATCAAGAGATAACTTAAT
AGAACTTTTAAAAATAACTTCTTTGTCTAATAGCGAATTAGATAGAATGCTTACAAAATATCCAAGAGTACCAATAATAA
CAGTGCATCAAGCAAAGGGTGCGGAATTTGATTATGTATTTTTAGCTGGACTTCAAAATAATATATTTCCAAATTATATG
GCAGTAAAAGAAGGAAATGAAATAGAAGAGAAAAGACTATTTTATGTAGCTATGACTAGAGCAAAAAAATATTTATATAT
AAGCTTTTCAGAATATGAACATAACAGGAGAAAAGCTAAAAGTATGCTTATAGATTATATACCTAAAGATTATTTAAAGT
ATATGTAA

Upstream 100 bases:

>100_bases
AAGATGTATATAGTGTTTCTTAATTATAGGTGGAGTTTTATTAAAAATAATATAAATACATTTAATAATAAAAATCAAGG
TATAATTGGAGGTGAATCTT

Downstream 100 bases:

>100_bases
TAAATATAAGTTAAAGCCACACTAAGAAAAATATAATTTAAATATTTTTACTTAATGTGGCTTTCTTATAGAATAATGCC
TATAGACATTATATTCCTCT

Product: helicase, UvrD/REP/exonuclease family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 855; Mature: 855

Protein sequence:

>855_residues
MLDIYLNDKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGNIIAKNLALGEQILCLTFTNRACKEMKEKIIETVGKEGLK
VTVKTFHSFCFDVIKKEAKKNTDISSDFTIYDEEDTKEIISELNSYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMI
KYNSNIFNDICKNEKYMVDKTLVDYLKIYGYLMVISYDKKLYERHGLDFNDLIIKVYDLFQKDKITTYWREKYKFIHIDE
MQDTSEIEYNIVSKLFRNNNIMLSGDYYQTIYEWRGSNPIIIFDKYKKEYNPEFIVFDKNYRSSEILLKASYEFLLNAFG
KDVNEIYKEGIEPKANIKGDLIEFKKAYDFMEEATFIFNKIKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKEE
RVGFLLVDEFKFFRREEIKDVLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
DYYELLTRGLDNENVVVFDVESTGINTTEDEIVQIAGIKINNKGEVIESFQRFLTPKKFVGDSYLVHGFSDQFLKENGEN
KKVVLKDFLEFIKDTVIVGHNVAFDISILNSELERLSLEKASFKTYYDTLDIARRFYPNLTNHKLETLSKLFNTETKSSH
DAMDDILATKDVLMAMLKEKVKPTIINRMVVYGKYIKKFQPIYKEITNLKKLTYIKNPKDIIGEIVNKTKIKEIYKDQYI
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKGAEFDYVFLAGLQNNIFPNYM
AVKEGNEIEEKRLFYVAMTRAKKYLYISFSEYEHNRRKAKSMLIDYIPKDYLKYM

Sequences:

>Translated_855_residues
MLDIYLNDKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGNIIAKNLALGEQILCLTFTNRACKEMKEKIIETVGKEGLK
VTVKTFHSFCFDVIKKEAKKNTDISSDFTIYDEEDTKEIISELNSYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMI
KYNSNIFNDICKNEKYMVDKTLVDYLKIYGYLMVISYDKKLYERHGLDFNDLIIKVYDLFQKDKITTYWREKYKFIHIDE
MQDTSEIEYNIVSKLFRNNNIMLSGDYYQTIYEWRGSNPIIIFDKYKKEYNPEFIVFDKNYRSSEILLKASYEFLLNAFG
KDVNEIYKEGIEPKANIKGDLIEFKKAYDFMEEATFIFNKIKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKEE
RVGFLLVDEFKFFRREEIKDVLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
DYYELLTRGLDNENVVVFDVESTGINTTEDEIVQIAGIKINNKGEVIESFQRFLTPKKFVGDSYLVHGFSDQFLKENGEN
KKVVLKDFLEFIKDTVIVGHNVAFDISILNSELERLSLEKASFKTYYDTLDIARRFYPNLTNHKLETLSKLFNTETKSSH
DAMDDILATKDVLMAMLKEKVKPTIINRMVVYGKYIKKFQPIYKEITNLKKLTYIKNPKDIIGEIVNKTKIKEIYKDQYI
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKGAEFDYVFLAGLQNNIFPNYM
AVKEGNEIEEKRLFYVAMTRAKKYLYISFSEYEHNRRKAKSMLIDYIPKDYLKYM
>Mature_855_residues
MLDIYLNDKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGNIIAKNLALGEQILCLTFTNRACKEMKEKIIETVGKEGLK
VTVKTFHSFCFDVIKKEAKKNTDISSDFTIYDEEDTKEIISELNSYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMI
KYNSNIFNDICKNEKYMVDKTLVDYLKIYGYLMVISYDKKLYERHGLDFNDLIIKVYDLFQKDKITTYWREKYKFIHIDE
MQDTSEIEYNIVSKLFRNNNIMLSGDYYQTIYEWRGSNPIIIFDKYKKEYNPEFIVFDKNYRSSEILLKASYEFLLNAFG
KDVNEIYKEGIEPKANIKGDLIEFKKAYDFMEEATFIFNKIKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKEE
RVGFLLVDEFKFFRREEIKDVLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
DYYELLTRGLDNENVVVFDVESTGINTTEDEIVQIAGIKINNKGEVIESFQRFLTPKKFVGDSYLVHGFSDQFLKENGEN
KKVVLKDFLEFIKDTVIVGHNVAFDISILNSELERLSLEKASFKTYYDTLDIARRFYPNLTNHKLETLSKLFNTETKSSH
DAMDDILATKDVLMAMLKEKVKPTIINRMVVYGKYIKKFQPIYKEITNLKKLTYIKNPKDIIGEIVNKTKIKEIYKDQYI
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKGAEFDYVFLAGLQNNIFPNYM
AVKEGNEIEEKRLFYVAMTRAKKYLYISFSEYEHNRRKAKSMLIDYIPKDYLKYM

Specific function: DNA helicase. Has a broad nucleotide specificity, even being able to hydrolyze ethenonucleotides, and is able to couple the hydrolysis to unwinding of DNA substrates. It is a 3'-5' helicase but at high protein concentrations it can also displace a substra

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=465, Percent_Identity=27.5268817204301, Blast_Score=176, Evalue=4e-45,
Organism=Escherichia coli, GI48994965, Length=470, Percent_Identity=26.1702127659574, Blast_Score=140, Evalue=4e-34,
Organism=Escherichia coli, GI1787196, Length=290, Percent_Identity=23.7931034482759, Blast_Score=90, Evalue=6e-19,
Organism=Escherichia coli, GI1786409, Length=172, Percent_Identity=26.7441860465116, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322369, Length=466, Percent_Identity=25.5364806866953, Blast_Score=105, Evalue=3e-23,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 100577; Mature: 100577

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDIYLNDKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGNIIAKNLALGEQILCLTFTN
CEEEEECCHHHHHHHHCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCEEEEEEECH
RACKEMKEKIIETVGKEGLKVTVKTFHSFCFDVIKKEAKKNTDISSDFTIYDEEDTKEII
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHH
SELNSYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMIKYNSNIFNDICKNEKYMVDK
HHHHCCEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCEEHHH
TLVDYLKIYGYLMVISYDKKLYERHGLDFNDLIIKVYDLFQKDKITTYWREKYKFIHIDE
HHHHHHHHHHHHEEEEHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCCEEEEEEC
MQDTSEIEYNIVSKLFRNNNIMLSGDYYQTIYEWRGSNPIIIFDKYKKEYNPEFIVFDKN
CCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCEEEEECCHHCCCCCEEEEECC
YRSSEILLKASYEFLLNAFGKDVNEIYKEGIEPKANIKGDLIEFKKAYDFMEEATFIFNK
CCCCCEEEEHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKEERVGFLLVDEFKFFRREEIKD
HHHHHHHHHHEEEEEEECCEEEEEEHHHHHHHHCCCCHHHCCCEEEEHHHHHHHHHHHHH
VLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHCHHHHH
DYYELLTRGLDNENVVVFDVESTGINTTEDEIVQIAGIKINNKGEVIESFQRFLTPKKFV
HHHHHHHCCCCCCCEEEEEECCCCCCCCHHHEEEEEEEEECCCCHHHHHHHHHCCCHHHC
GDSYLVHGFSDQFLKENGENKKVVLKDFLEFIKDTVIVGHNVAFDISILNSELERLSLEK
CCCCEEECCCHHHHHCCCCCCEEHHHHHHHHHHHHEEECCCEEEEHHHHHHHHHHHHHHH
ASFKTYYDTLDIARRFYPNLTNHKLETLSKLFNTETKSSHDAMDDILATKDVLMAMLKEK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VKPTIINRMVVYGKYIKKFQPIYKEITNLKKLTYIKNPKDIIGEIVNKTKIKEIYKDQYI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCE
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKG
EECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCC
AEFDYVFLAGLQNNIFPNYMAVKEGNEIEEKRLFYVAMTRAKKYLYISFSEYEHNRRKAK
CCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHEEEECCCEEEEEEEHHHHHHHHHHH
SMLIDYIPKDYLKYM
HHHHHHCCHHHHHCC
>Mature Secondary Structure
MLDIYLNDKQKKVINTLDKNILLLSSAGTGKTKTLSMRIGNIIAKNLALGEQILCLTFTN
CEEEEECCHHHHHHHHCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHHCCCEEEEEEECH
RACKEMKEKIIETVGKEGLKVTVKTFHSFCFDVIKKEAKKNTDISSDFTIYDEEDTKEII
HHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCHHHHHHH
SELNSYKFNVSCLQRFINIVKEKSINYKDRDYKDVIDTMIKYNSNIFNDICKNEKYMVDK
HHHHCCEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCHHHHHHHCCCCEEHHH
TLVDYLKIYGYLMVISYDKKLYERHGLDFNDLIIKVYDLFQKDKITTYWREKYKFIHIDE
HHHHHHHHHHHHEEEEHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHCCEEEEEEC
MQDTSEIEYNIVSKLFRNNNIMLSGDYYQTIYEWRGSNPIIIFDKYKKEYNPEFIVFDKN
CCCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCCCEEEEECCHHCCCCCEEEEECC
YRSSEILLKASYEFLLNAFGKDVNEIYKEGIEPKANIKGDLIEFKKAYDFMEEATFIFNK
CCCCCEEEEHHHHHHHHHHCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IKSLKVEKLSRVAILTRNNRINIALSEKFKILNSKLPKEERVGFLLVDEFKFFRREEIKD
HHHHHHHHHHEEEEEEECCEEEEEEHHHHHHHHCCCCHHHCCCEEEEHHHHHHHHHHHHH
VLAYLKLIVNDFDSNSLKRILKKFGKGIGERTIEEIEKEENIKIGVRLTDLIHKNTQEYG
HHHHHHHHHHCCCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEEHHHHHHCHHHHH
DYYELLTRGLDNENVVVFDVESTGINTTEDEIVQIAGIKINNKGEVIESFQRFLTPKKFV
HHHHHHHCCCCCCCEEEEEECCCCCCCCHHHEEEEEEEEECCCCHHHHHHHHHCCCHHHC
GDSYLVHGFSDQFLKENGENKKVVLKDFLEFIKDTVIVGHNVAFDISILNSELERLSLEK
CCCCEEECCCHHHHHCCCCCCEEHHHHHHHHHHHHEEECCCEEEEHHHHHHHHHHHHHHH
ASFKTYYDTLDIARRFYPNLTNHKLETLSKLFNTETKSSHDAMDDILATKDVLMAMLKEK
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VKPTIINRMVVYGKYIKKFQPIYKEITNLKKLTYIKNPKDIIGEIVNKTKIKEIYKDQYI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCE
KINNLREFFVIAKEIENKEISSRDNLIELLKITSLSNSELDRMLTKYPRVPIITVHQAKG
EECCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEECCCC
AEFDYVFLAGLQNNIFPNYMAVKEGNEIEEKRLFYVAMTRAKKYLYISFSEYEHNRRKAK
CCCCEEEEECCCCCCCCCEEEECCCCCHHHHHHHHEEEECCCEEEEEEEHHHHHHHHHHH
SMLIDYIPKDYLKYM
HHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9592155; 8934527; 10199404; 10388562; 10454638 [H]