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The map label for this gene is topB [H]

Identifier: 148379270

GI number: 148379270

Start: 1400879

End: 1403074

Strand: Direct

Name: topB [H]

Synonym: CBO1289

Alternate gene names: 148379270

Gene position: 1400879-1403074 (Clockwise)

Preceding gene: 148379269

Following gene: 148379271

Centisome position: 36.04

GC content: 30.01

Gene sequence:

>2196_bases
ATGGGAAAAGTATTAGTTTTAGCAGAAAAACCTAGTGTAGGAAGAGATTTGGCTAAGGTTTTAAAATGCAATAAAAAGGG
TAATGGTTATTTAGAAGGAAATAAATATATTGTAACTTGGGCTTTAGGACATTTAATAACTTTATCTGATCCTGAAAGTT
ATGATGAAAAATATAAAAAATGGAGCATGGATACTCTTCCAATGCTACCTAAGAGAATGAAAACTACAGTTATAAAGAAG
ACTTCAAAGCATTTTAGTGAAGTTAGAAAAGTTATGATAAGAAAAGATGTAGAAGAACTTGTTATAGCTACAGATGCAGG
AAGAGAAGGAGAGCTTGTAGCTAGATGGATAATTGATAAAGTAGGTTTTAAAAAGCCTATAAAACGTCTTTGGATTTCTT
CACAAACAGATAAAGCTATTTTAGACGGATTTAGAAATTTAAAACCGGGAAAAAATTATGAGAATTTATATCATTCAGCA
GTGTGCAGATCAGAAGCAGATTGGATTGTAGGACTTAATGTTACGAGAGCTTTGACTTGTAGATATAATGCTCAACTTTC
AGCAGGAAGAGTTCAAACACCTACTCTAGCTATGATAGTTCAAAGAGAAGAGGAAATAAAGAATTTTAAACCTAGAGATT
ATTATAGCATAGAAGGTAAGACTAAAGGATTTACAATGCATTGGGAGAGTGCTAAGGGTAGATTTAATACCTTTGATGAA
GATTTAGCTAAAAAAGTAGTTTCAAAAGTTACAGGTAAAGAGGGAAAAATAATAGAGGTAACGGAAAGTGATAAAAAGAA
ATATGCTCCGGCTCTTTATGATTTAACAGAACTTCAAAGGGATGCTAATAGAATATTTGGATATTCAGCAAAACAAACCC
TATCCATAATGCAAAGATTATATGAGAATCATAAAATTTTAACTTATCCAAGAACAGATTCTAGATATATTTCAAAGGAT
GTTGTTGGTACTTTAAAAGATAGATTAAAGGCTATATCCATTAGTAATTATAGTAAGTTTACAAATGAAATATTAAAAGG
AAACATTAAGGCACATAAAGGTTTTGTAGATGATAGTAAGGTTTCAGACCATCATGCTATAATTCCTACAGAAGAAAAAC
CTAAATTAGGTAACCTAAGTGGTGAGGAAAGAAATATTTATGATTTAGTTATTAAAAGATTTTTATCAGTTATGTTACCA
CCCTTTGAATATATTCAAACTACCATTAAATCAGAAGTTATAGGTGAAAAATTTGTAGCTAGAGGAAAGGTTATCAAATC
CAAGGGATGGAAAGCAGTTTATGATAGAGAAGAATTCGATGATAAAAATAATAATTCAGATAGTGATGATATAAAAGATC
AAACTCTTCCTAAGGTTAATAAGGGAGATGGTATAAAATTTTCTAACTTTAAGATAAACAAAGGTCAAACAAAGCCGCCA
GCTAGATTCAATGAGGGAACTTTATTATCTGCTATGGAGAATCCTCAAAGATATATTAATTTAGATAAGGAGAGTTCTAA
AACCTTAGGAGAAACTGGTGGACTTGGAACTGTTGCAACTAGAGCAGATATAATTGAAAAATTATATAATACTTTTTATA
TAGAAAAAAGAGGAAAAGAAATAGTTCCAACTTCAAAGGGAAAGCAAGTTATAGATTTAGTACCTAAGGATTTGAAATCC
CCACTTTTAACAGCGAAATGGGAAAGAGAATTAGATTCCATTAGTAAGGGTAATTTAAGAGGACAGATATTTATAAATAA
AATGAGAGACTATTCTACAAAACTTGTAGAAGATGTAAAAAATAGCAAGGATAAATATGTTCATGATAATTTAACTGGTA
AAAAATGTCCACAATGTGGCAAATATATGTTAGAAGTTAAGGGTAAAAATGGAGTTATAAATGTATGCCAGGATAGAGAA
TGTGGCTATAGAGAAAATGTAAGCAGATTTACCAATGTAAGATGTCCTGAGTGTCATGTAAAATTAGAACTTAGAGGAGA
AGGGCAGGGTCAAATCTATGTATGTACAAGATGTAGTTTTAGAGAAAAACTTTCTTCCTTTAATAAAAAGTATAGAAACA
ATCAAGAAAAATTAAATAAAAGAGATGTAACTAAATATATGAAAAAAATGCAAAAAGAAAATGACGAACCAATAAATTCA
GCTTTAGCAGAGGCTTTATCTAAATTGAAATTATAA

Upstream 100 bases:

>100_bases
TTAATATATAATTATATATATGTAATGTAAGGGTAGTAAGTATATCTATCCTTATTTTTTATTAAAAAAGGAATTATTTA
TGGAAGGACGATAATTATTT

Downstream 100 bases:

>100_bases
AGAATATGCTATAATAGATGTAATTTTAAATAAGAGAATATATTTTTCAAATAAAAATTCCTTGACCTTATAGTCCAATA
TACAATATAATTAAAACGAA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 731; Mature: 730

Protein sequence:

>731_residues
MGKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKKWSMDTLPMLPKRMKTTVIKK
TSKHFSEVRKVMIRKDVEELVIATDAGREGELVARWIIDKVGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSA
VCRSEADWIVGLNVTRALTCRYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFTMHWESAKGRFNTFDE
DLAKKVVSKVTGKEGKIIEVTESDKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRLYENHKILTYPRTDSRYISKD
VVGTLKDRLKAISISNYSKFTNEILKGNIKAHKGFVDDSKVSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLP
PFEYIQTTIKSEVIGEKFVARGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFKINKGQTKPP
ARFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKEIVPTSKGKQVIDLVPKDLKS
PLLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVKNSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDRE
CGYRENVSRFTNVRCPECHVKLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVTKYMKKMQKENDEPINS
ALAEALSKLKL

Sequences:

>Translated_731_residues
MGKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKKWSMDTLPMLPKRMKTTVIKK
TSKHFSEVRKVMIRKDVEELVIATDAGREGELVARWIIDKVGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSA
VCRSEADWIVGLNVTRALTCRYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFTMHWESAKGRFNTFDE
DLAKKVVSKVTGKEGKIIEVTESDKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRLYENHKILTYPRTDSRYISKD
VVGTLKDRLKAISISNYSKFTNEILKGNIKAHKGFVDDSKVSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLP
PFEYIQTTIKSEVIGEKFVARGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFKINKGQTKPP
ARFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKEIVPTSKGKQVIDLVPKDLKS
PLLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVKNSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDRE
CGYRENVSRFTNVRCPECHVKLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVTKYMKKMQKENDEPINS
ALAEALSKLKL
>Mature_730_residues
GKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKKWSMDTLPMLPKRMKTTVIKKT
SKHFSEVRKVMIRKDVEELVIATDAGREGELVARWIIDKVGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSAV
CRSEADWIVGLNVTRALTCRYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFTMHWESAKGRFNTFDED
LAKKVVSKVTGKEGKIIEVTESDKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRLYENHKILTYPRTDSRYISKDV
VGTLKDRLKAISISNYSKFTNEILKGNIKAHKGFVDDSKVSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLPP
FEYIQTTIKSEVIGEKFVARGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFKINKGQTKPPA
RFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKEIVPTSKGKQVIDLVPKDLKSP
LLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVKNSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDREC
GYRENVSRFTNVRCPECHVKLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVTKYMKKMQKENDEPINSA
LAEALSKLKL

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=754, Percent_Identity=22.8116710875332, Blast_Score=146, Evalue=6e-35,
Organism=Homo sapiens, GI10835218, Length=692, Percent_Identity=24.5664739884393, Blast_Score=142, Evalue=1e-33,
Organism=Escherichia coli, GI1788061, Length=621, Percent_Identity=31.8840579710145, Blast_Score=343, Evalue=3e-95,
Organism=Escherichia coli, GI1787529, Length=704, Percent_Identity=25, Blast_Score=164, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI32563869, Length=694, Percent_Identity=24.9279538904899, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17555378, Length=591, Percent_Identity=25.7191201353638, Blast_Score=120, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=702, Percent_Identity=22.3646723646724, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI24640096, Length=715, Percent_Identity=24.7552447552448, Blast_Score=135, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24585251, Length=568, Percent_Identity=23.943661971831, Blast_Score=118, Evalue=2e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 83654; Mature: 83523

Theoretical pI: Translated: 10.09; Mature: 10.09

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKK
CCCEEEEECCCCCHHHHHHHHHCCCCCCCEECCCCEEEEEECCCEEECCCCHHHHHHHHH
WSMDTLPMLPKRMKTTVIKKTSKHFSEVRKVMIRKDVEELVIATDAGREGELVARWIIDK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHH
VGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSAVCRSEADWIVGLNVTRALTC
HCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEEE
RYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFTMHWESAKGRFNTFDE
EECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCCCCCHHH
DLAKKVVSKVTGKEGKIIEVTESDKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRL
HHHHHHHHHHCCCCCCEEEEECCCCHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YENHKILTYPRTDSRYISKDVVGTLKDRLKAISISNYSKFTNEILKGNIKAHKGFVDDSK
HHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHCCCCCCCC
VSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLPPFEYIQTTIKSEVIGEKFVA
CCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFKINKGQTKPP
CCCEECCCCCCEECCCHHCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEEEECCCCCCCC
ARFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKE
CCCCCCCEEHHHHCCHHEEECCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCC
IVPTSKGKQVIDLVPKDLKSPLLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVK
CCCCCCCCHHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHH
NSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDRECGYRENVSRFTNVRCPECHV
CCCCCEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEE
KLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVTKYMKKMQKENDEPINS
EEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
ALAEALSKLKL
HHHHHHHHHCC
>Mature Secondary Structure 
GKVLVLAEKPSVGRDLAKVLKCNKKGNGYLEGNKYIVTWALGHLITLSDPESYDEKYKK
CCEEEEECCCCCHHHHHHHHHCCCCCCCEECCCCEEEEEECCCEEECCCCHHHHHHHHH
WSMDTLPMLPKRMKTTVIKKTSKHFSEVRKVMIRKDVEELVIATDAGREGELVARWIIDK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHH
VGFKKPIKRLWISSQTDKAILDGFRNLKPGKNYENLYHSAVCRSEADWIVGLNVTRALTC
HCHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEEE
RYNAQLSAGRVQTPTLAMIVQREEEIKNFKPRDYYSIEGKTKGFTMHWESAKGRFNTFDE
EECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEEECCCCCCCCCHHH
DLAKKVVSKVTGKEGKIIEVTESDKKKYAPALYDLTELQRDANRIFGYSAKQTLSIMQRL
HHHHHHHHHHCCCCCCEEEEECCCCHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
YENHKILTYPRTDSRYISKDVVGTLKDRLKAISISNYSKFTNEILKGNIKAHKGFVDDSK
HHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHCCCCCCCC
VSDHHAIIPTEEKPKLGNLSGEERNIYDLVIKRFLSVMLPPFEYIQTTIKSEVIGEKFVA
CCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
RGKVIKSKGWKAVYDREEFDDKNNNSDSDDIKDQTLPKVNKGDGIKFSNFKINKGQTKPP
CCCEECCCCCCEECCCHHCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEEEECCCCCCCC
ARFNEGTLLSAMENPQRYINLDKESSKTLGETGGLGTVATRADIIEKLYNTFYIEKRGKE
CCCCCCCEEHHHHCCHHEEECCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCC
IVPTSKGKQVIDLVPKDLKSPLLTAKWERELDSISKGNLRGQIFINKMRDYSTKLVEDVK
CCCCCCCCHHHHHCCHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHHHHH
NSKDKYVHDNLTGKKCPQCGKYMLEVKGKNGVINVCQDRECGYRENVSRFTNVRCPECHV
CCCCCEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHCCCCCCEEE
KLELRGEGQGQIYVCTRCSFREKLSSFNKKYRNNQEKLNKRDVTKYMKKMQKENDEPINS
EEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
ALAEALSKLKL
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA