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The map label for this gene is grdE [H]

Identifier: 148379241

GI number: 148379241

Start: 1372234

End: 1373520

Strand: Direct

Name: grdE [H]

Synonym: CBO1256

Alternate gene names: 148379241

Gene position: 1372234-1373520 (Clockwise)

Preceding gene: 148379240

Following gene: 148379242

Centisome position: 35.3

GC content: 32.71

Gene sequence:

>1287_bases
ATGCGTTTAGAAATTGGAAAAATATTTATAAGTGATATGCAGTTTTCCAATGAAACAAAAGTTAAAGATGGAGTCTTGTA
TATTTCTAAAGAAGAACTTCTAAAAGAAATTGGTACCGACGAGAGAATTAAAAGTATTGATTTAGAAATAGCTAAGCCAG
GAGATAAAACTAGAATTATTCCAGTAAAAGATGTAATAGAGCCAAGAGTTAAAGTAGAAGGAAATGGTGGTATTTTCCCA
GGCTTTATAAGTAAAGTTGATACTGTTGGTTCAGGCAAAACTAATGTATTAAAGGGAGCCGCAGTAGTAACCACAGGTAA
AATAGTAGGGTTCCAAGAAGGTATTATTGATATGAGTGGGGAAGGAGCTAAATATACACCTTTTTCAAAAACTAATAATT
TAGTTGTAGTATGTGAACCTAAAGAAGGAGTAAATCAATACGAACATGAAGAAATTGTAAGAACTTTAGGGTTTAAAGCT
GCTACTTATTTAGGTAGTCTTGCAAAAGATATTACACCAGATGAAACTAAGGTTTATGAAACATTACCTTTATTAGAACA
AGTAAAGAAATATCCAGACCTTCCTAAGGTAGTATATGTTTATATGCTTCAAAGCCAAGGACTTTTACATGATACCTATG
TATATGGAGTAGATGCTAAAAAAATAATACCAACATTTATATATCCAACAGAAGTATTTGATGGTGCCATAGTTAGTGGA
AATTGTGTTTCAGCTTGTGACAAGAATCCAAGTTATGTACACATGAACCATCCTGTTATTGAAGATTTATATGAGAAACA
TGGAGTAGAATATAATTTTTTAGGTTGTGTTATAACAAATGAAAACGTTTATTTGGCGGATAAAGTTAGATCTTCAAGTT
ATACAGCTAAGTTAGTAGAATTTTTAGGAGCAGATGCAGTAATAATTTCAGAGGAAGGTTTTGGAAATCCAGATGCAGAC
CTTGTTATGAATTGTAATAAGATAAGTGAGAAAGGTATAAAAACAGTATTAATAACAGATGAGTATGCAGGACAAAATGG
AGCTTCACAATCCTTAGCAGATTCTACACCAAAGGGTGATGCAGTAGTTACAGGAGGAAATGCTAATGAGGTTGTAACAT
TACCACCAATGGAAAAAATTATTGGTCATGTTGAGGTAGCAGATGTAATTGCAGGTGGACATGTTGGTTCTTTAAAAGAA
GATGGTTCTATAGAGGCAGAGATTCAAGTTATTACAGGAGCTACTAGTGAAGTTGGCTTTAACTATTTAAGTGCTAAAGG
CTATTAA

Upstream 100 bases:

>100_bases
ACTAAATTAATTTTATATAAGATTTATATAAAAATTGCTAGAATCATTTAGCAGTACAATAAAGATAAGGCATTAAAAAT
TTAATTTGGGGGTTGATATT

Downstream 100 bases:

>100_bases
ATTGTAGTTATATAATCAAGGGGGGAAAATAAATGATTAAAGTAGTTCATTATATAAATCAGTTTTATGCGGGTATTGGA
GGAGAAGACAAAGCGGATTA

Product: glycine reductase complex component B, alpha and beta subunits

Products: NA

Alternate protein names: Selenoprotein PB alpha/beta [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MRLEIGKIFISDMQFSNETKVKDGVLYISKEELLKEIGTDERIKSIDLEIAKPGDKTRIIPVKDVIEPRVKVEGNGGIFP
GFISKVDTVGSGKTNVLKGAAVVTTGKIVGFQEGIIDMSGEGAKYTPFSKTNNLVVVCEPKEGVNQYEHEEIVRTLGFKA
ATYLGSLAKDITPDETKVYETLPLLEQVKKYPDLPKVVYVYMLQSQGLLHDTYVYGVDAKKIIPTFIYPTEVFDGAIVSG
NCVSACDKNPSYVHMNHPVIEDLYEKHGVEYNFLGCVITNENVYLADKVRSSSYTAKLVEFLGADAVIISEEGFGNPDAD
LVMNCNKISEKGIKTVLITDEYAGQNGASQSLADSTPKGDAVVTGGNANEVVTLPPMEKIIGHVEVADVIAGGHVGSLKE
DGSIEAEIQVITGATSEVGFNYLSAKGY

Sequences:

>Translated_428_residues
MRLEIGKIFISDMQFSNETKVKDGVLYISKEELLKEIGTDERIKSIDLEIAKPGDKTRIIPVKDVIEPRVKVEGNGGIFP
GFISKVDTVGSGKTNVLKGAAVVTTGKIVGFQEGIIDMSGEGAKYTPFSKTNNLVVVCEPKEGVNQYEHEEIVRTLGFKA
ATYLGSLAKDITPDETKVYETLPLLEQVKKYPDLPKVVYVYMLQSQGLLHDTYVYGVDAKKIIPTFIYPTEVFDGAIVSG
NCVSACDKNPSYVHMNHPVIEDLYEKHGVEYNFLGCVITNENVYLADKVRSSSYTAKLVEFLGADAVIISEEGFGNPDAD
LVMNCNKISEKGIKTVLITDEYAGQNGASQSLADSTPKGDAVVTGGNANEVVTLPPMEKIIGHVEVADVIAGGHVGSLKE
DGSIEAEIQVITGATSEVGFNYLSAKGY
>Mature_428_residues
MRLEIGKIFISDMQFSNETKVKDGVLYISKEELLKEIGTDERIKSIDLEIAKPGDKTRIIPVKDVIEPRVKVEGNGGIFP
GFISKVDTVGSGKTNVLKGAAVVTTGKIVGFQEGIIDMSGEGAKYTPFSKTNNLVVVCEPKEGVNQYEHEEIVRTLGFKA
ATYLGSLAKDITPDETKVYETLPLLEQVKKYPDLPKVVYVYMLQSQGLLHDTYVYGVDAKKIIPTFIYPTEVFDGAIVSG
NCVSACDKNPSYVHMNHPVIEDLYEKHGVEYNFLGCVITNENVYLADKVRSSSYTAKLVEFLGADAVIISEEGFGNPDAD
LVMNCNKISEKGIKTVLITDEYAGQNGASQSLADSTPKGDAVVTGGNANEVVTLPPMEKIIGHVEVADVIAGGHVGSLKE
DGSIEAEIQVITGATSEVGFNYLSAKGY

Specific function: In the first step of glycine reductase, the substrate is bound to component PB via a Schiff base intermediate. Then the PB- activated substrate is nucleophilically attacked by the selenol anion of component PA to transform it to a carboxymethylated seleno

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016585
- InterPro:   IPR015417 [H]

Pfam domain/function: PF09338 Gly_reductase [H]

EC number: =1.21.4.2 [H]

Molecular weight: Translated: 46339; Mature: 46339

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLEIGKIFISDMQFSNETKVKDGVLYISKEELLKEIGTDERIKSIDLEIAKPGDKTRII
CEEEEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHCCCCCCEEEEEEEEECCCCCEEEE
PVKDVIEPRVKVEGNGGIFPGFISKVDTVGSGKTNVLKGAAVVTTGKIVGFQEGIIDMSG
EHHHHCCCCEEECCCCCCCCHHHHHHHCCCCCCCCEECCEEEEEECEEEEECCCCEEECC
EGAKYTPFSKTNNLVVVCEPKEGVNQYEHEEIVRTLGFKAATYLGSLAKDITPDETKVYE
CCCEECCCCCCCCEEEEECCHHCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHH
TLPLLEQVKKYPDLPKVVYVYMLQSQGLLHDTYVYGVDAKKIIPTFIYPTEVFDGAIVSG
HHHHHHHHHHCCCCCHHEEEEEHHCCCCEEEEEEEECCHHHHCCEEEECHHHCCCEEECC
NCVSACDKNPSYVHMNHPVIEDLYEKHGVEYNFLGCVITNENVYLADKVRSSSYTAKLVE
CCHHHCCCCCCEEEECCCHHHHHHHHHCCCEEEEEEEEECCCEEEEECCCCCHHHHHHHH
FLGADAVIISEEGFGNPDADLVMNCNKISEKGIKTVLITDEYAGQNGASQSLADSTPKGD
HHCCCEEEEECCCCCCCCHHEEEECHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
AVVTGGNANEVVTLPPMEKIIGHVEVADVIAGGHVGSLKEDGSIEAEIQVITGATSEVGF
EEEECCCCCCEEECCCHHHHHCCCEEEHHHCCCCCCCCCCCCCCEEEEEEEECCCHHHCC
NYLSAKGY
EEEECCCC
>Mature Secondary Structure
MRLEIGKIFISDMQFSNETKVKDGVLYISKEELLKEIGTDERIKSIDLEIAKPGDKTRII
CEEEEEEEEEECCCCCCCCCCCCCEEEEEHHHHHHHCCCCCCEEEEEEEEECCCCCEEEE
PVKDVIEPRVKVEGNGGIFPGFISKVDTVGSGKTNVLKGAAVVTTGKIVGFQEGIIDMSG
EHHHHCCCCEEECCCCCCCCHHHHHHHCCCCCCCCEECCEEEEEECEEEEECCCCEEECC
EGAKYTPFSKTNNLVVVCEPKEGVNQYEHEEIVRTLGFKAATYLGSLAKDITPDETKVYE
CCCEECCCCCCCCEEEEECCHHCCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHH
TLPLLEQVKKYPDLPKVVYVYMLQSQGLLHDTYVYGVDAKKIIPTFIYPTEVFDGAIVSG
HHHHHHHHHHCCCCCHHEEEEEHHCCCCEEEEEEEECCHHHHCCEEEECHHHCCCEEECC
NCVSACDKNPSYVHMNHPVIEDLYEKHGVEYNFLGCVITNENVYLADKVRSSSYTAKLVE
CCHHHCCCCCCEEEECCCHHHHHHHHHCCCEEEEEEEEECCCEEEEECCCCCHHHHHHHH
FLGADAVIISEEGFGNPDADLVMNCNKISEKGIKTVLITDEYAGQNGASQSLADSTPKGD
HHCCCEEEEECCCCCCCCHHEEEECHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
AVVTGGNANEVVTLPPMEKIIGHVEVADVIAGGHVGSLKEDGSIEAEIQVITGATSEVGF
EEEECCCCCCEEECCCHHHHHCCCEEEHHHCCCCCCCCCCCCCCEEEEEEEECCCHHHCC
NYLSAKGY
EEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11271425 [H]