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The map label for this gene is puuR [H]

Identifier: 148379223

GI number: 148379223

Start: 1357709

End: 1358242

Strand: Reverse

Name: puuR [H]

Synonym: CBO1240

Alternate gene names: 148379223

Gene position: 1358242-1357709 (Counterclockwise)

Preceding gene: 148379228

Following gene: 148379221

Centisome position: 34.94

GC content: 27.72

Gene sequence:

>534_bases
ATGCAGTTAGGTGAAAAGATTAAATATTACAGAAAGAAAAAAGGTCTTACCATAAAAGAATTATCAGAATTAACAAACCT
ATCTATAGGTTTTATAAGCAATTTAGAAAGAGATTTGAATAGTCCTTCTGTAAGTAATTTACAGCAAATTTGTGAAGTGC
TCGGAATAAATTTGATGGAAATATTAAAATCTACAGAAGATAAAGAATATATTGTATGTAAGGACAGTCGTAGTGAAATT
TTTTCTACAGATGATAAAAAAATAAAATTCGAAATGTTAACTAATGGAAATAAAAATTTAAATGCCATAGCTATAACTAT
AGAAGGAAATACAGACTATAATGATACTTCCTGGGGGCATAGTTATGATGAATTGGGTATTGTAGTAAAAGGTACCTTAG
AAATCCAGATGAACTCTCAGACTTATACTCTTCACGAAGGAGATAGTATTTATTTAAATAAATTTACTCCTCATAGGTAT
AAAAATCCTTACGAAGAGATTAATGTAACCTATTGGTTTTCTGTAAAAGAATAA

Upstream 100 bases:

>100_bases
TTGAATAACAAACTTAAATAATACTTTCCAAAATATTTAAGAAGTTATGTTTTTTATGCTATAATTAAAGTAAATTATAA
AACTACATTAGGAGGCTAAA

Downstream 100 bases:

>100_bases
AAAAATAATATGATACATATGGTTTATTTATTATAAATTTACTTTTAAATATAAAAAGTAAATTCCTTTGAGAAAATTAA
ATACAACTTTCAATAATAGA

Product: DNA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MQLGEKIKYYRKKKGLTIKELSELTNLSIGFISNLERDLNSPSVSNLQQICEVLGINLMEILKSTEDKEYIVCKDSRSEI
FSTDDKKIKFEMLTNGNKNLNAIAITIEGNTDYNDTSWGHSYDELGIVVKGTLEIQMNSQTYTLHEGDSIYLNKFTPHRY
KNPYEEINVTYWFSVKE

Sequences:

>Translated_177_residues
MQLGEKIKYYRKKKGLTIKELSELTNLSIGFISNLERDLNSPSVSNLQQICEVLGINLMEILKSTEDKEYIVCKDSRSEI
FSTDDKKIKFEMLTNGNKNLNAIAITIEGNTDYNDTSWGHSYDELGIVVKGTLEIQMNSQTYTLHEGDSIYLNKFTPHRY
KNPYEEINVTYWFSVKE
>Mature_177_residues
MQLGEKIKYYRKKKGLTIKELSELTNLSIGFISNLERDLNSPSVSNLQQICEVLGINLMEILKSTEDKEYIVCKDSRSEI
FSTDDKKIKFEMLTNGNKNLNAIAITIEGNTDYNDTSWGHSYDELGIVVKGTLEIQMNSQTYTLHEGDSIYLNKFTPHRY
KNPYEEINVTYWFSVKE

Specific function: Represses puuD [H]

COG id: COG1396

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH cro/C1-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787557, Length=163, Percent_Identity=27.6073619631902, Blast_Score=75, Evalue=3e-15,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013096
- InterPro:   IPR011051
- InterPro:   IPR001387
- InterPro:   IPR010982
- InterPro:   IPR014710 [H]

Pfam domain/function: PF07883 Cupin_2; PF01381 HTH_3 [H]

EC number: NA

Molecular weight: Translated: 20453; Mature: 20453

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50943 HTH_CROC1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLGEKIKYYRKKKGLTIKELSELTNLSIGFISNLERDLNSPSVSNLQQICEVLGINLME
CCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHH
ILKSTEDKEYIVCKDSRSEIFSTDDKKIKFEMLTNGNKNLNAIAITIEGNTDYNDTSWGH
HHHCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCC
SYDELGIVVKGTLEIQMNSQTYTLHEGDSIYLNKFTPHRYKNPYEEINVTYWFSVKE
CCCCCCEEEEEEEEEEECCEEEEEECCCEEEEECCCCCHHCCCHHHEEEEEEEEECC
>Mature Secondary Structure
MQLGEKIKYYRKKKGLTIKELSELTNLSIGFISNLERDLNSPSVSNLQQICEVLGINLME
CCCCHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHH
ILKSTEDKEYIVCKDSRSEIFSTDDKKIKFEMLTNGNKNLNAIAITIEGNTDYNDTSWGH
HHHCCCCCEEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEEECCCCCCCCCCCC
SYDELGIVVKGTLEIQMNSQTYTLHEGDSIYLNKFTPHRYKNPYEEINVTYWFSVKE
CCCCCCEEEEEEEEEEECCEEEEEECCCEEEEECCCCCHHCCCHHHEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553 [H]