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The map label for this gene is cysK2 [H]

Identifier: 148379164

GI number: 148379164

Start: 1293422

End: 1294351

Strand: Direct

Name: cysK2 [H]

Synonym: CBO1179

Alternate gene names: 148379164

Gene position: 1293422-1294351 (Clockwise)

Preceding gene: 148379163

Following gene: 148379165

Centisome position: 33.28

GC content: 32.26

Gene sequence:

>930_bases
ATGAAAAAAATATATAAAAATATAACAGAATTAATAGGAAATACACCATTATTAGAATTAGAAAAATTAAAAAAAGAAAA
TAAATTAAAAGCTAATATAATAGCTAAAGTAGAATATTTTAATCCGGCAAACAGTGTAAAAGATAGAATAGCCTTCTCCA
TGATAGAAGAAGCAGAAAAAGAAGGATTAATAGATAAAGACACAGTAATAATAGAACCTACTAGTGGTAATACAGGAGTA
GGACTTGCTTTTGTAGCAGCGGCTAAAGGGTATAAACTTATACTAACTATGCCAGAAACTATGAGTATGGAAAGAAGACT
TATATTAAAAGCCTATGGTGCCGAGTTAGTCTTAACACCGGGAAAGGATGGAATGAAGGGGGCTATAGAAAAAGCAACAG
AATTATCAAAGGAATATAAAAATTCTTTTGTTCCGCAGCAATTTGAAAATAAATTTAATCCAGCTATGCATAAGAGGACT
ACAGCGGTAGAAATTTGGAATGATACAGACGGGGAAGTAGATATATTCATAGCAGGAGTAGGTACAGGTGGAACAATTAC
AGGAGTAGGGGAATATCTAAAAGAAAAAAATAAGGATATAAAAATAATAGCAGTAGAACCAGAAGATTCACCGGTTTTAT
CAGGAGGAAATCCAGGACCACATAAAATTCAAGGAATAGGAGCTGGATTTGTACCAAGTACTTTAAACACAGAGGTTTTA
GATGAAATATTTAAAGTATCTAATGAAAAAGCTTTTGAAGTAACAAAGAGTATAGCAAAAACAGAAGGCCTTTTAGTTGG
TATATCTTCAGGAGCAGCAATTTATGCGGCTATGAAAATAGCTGAAAGACAAGAAAATCAAGGCAAAAATATCGTGGTAT
TACTTCCAGATACAGGACAAAGATATTTATCTACAGGAGTATTTGAGTAA

Upstream 100 bases:

>100_bases
CAGTAGAAATTAAAAATGAAATTTTAATCTAAAACATAGTAAACCAATAGGCAAACTGATACCATCATATAATATTTAGC
ATTTAGAGGAGAGAATAATT

Downstream 100 bases:

>100_bases
TAGAAAAATAATTAATGAGGAAACCATAGATAGGAGGAAAAATTGTGGAAATTTTTGATGCAAAATATTTAGTACAATCA
TTAATAGTTCTTTTAAAGTA

Product: cysteine synthase A

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MKKIYKNITELIGNTPLLELEKLKKENKLKANIIAKVEYFNPANSVKDRIAFSMIEEAEKEGLIDKDTVIIEPTSGNTGV
GLAFVAAAKGYKLILTMPETMSMERRLILKAYGAELVLTPGKDGMKGAIEKATELSKEYKNSFVPQQFENKFNPAMHKRT
TAVEIWNDTDGEVDIFIAGVGTGGTITGVGEYLKEKNKDIKIIAVEPEDSPVLSGGNPGPHKIQGIGAGFVPSTLNTEVL
DEIFKVSNEKAFEVTKSIAKTEGLLVGISSGAAIYAAMKIAERQENQGKNIVVLLPDTGQRYLSTGVFE

Sequences:

>Translated_309_residues
MKKIYKNITELIGNTPLLELEKLKKENKLKANIIAKVEYFNPANSVKDRIAFSMIEEAEKEGLIDKDTVIIEPTSGNTGV
GLAFVAAAKGYKLILTMPETMSMERRLILKAYGAELVLTPGKDGMKGAIEKATELSKEYKNSFVPQQFENKFNPAMHKRT
TAVEIWNDTDGEVDIFIAGVGTGGTITGVGEYLKEKNKDIKIIAVEPEDSPVLSGGNPGPHKIQGIGAGFVPSTLNTEVL
DEIFKVSNEKAFEVTKSIAKTEGLLVGISSGAAIYAAMKIAERQENQGKNIVVLLPDTGQRYLSTGVFE
>Mature_309_residues
MKKIYKNITELIGNTPLLELEKLKKENKLKANIIAKVEYFNPANSVKDRIAFSMIEEAEKEGLIDKDTVIIEPTSGNTGV
GLAFVAAAKGYKLILTMPETMSMERRLILKAYGAELVLTPGKDGMKGAIEKATELSKEYKNSFVPQQFENKFNPAMHKRT
TAVEIWNDTDGEVDIFIAGVGTGGTITGVGEYLKEKNKDIKIIAVEPEDSPVLSGGNPGPHKIQGIGAGFVPSTLNTEVL
DEIFKVSNEKAFEVTKSIAKTEGLLVGISSGAAIYAAMKIAERQENQGKNIVVLLPDTGQRYLSTGVFE

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=310, Percent_Identity=41.9354838709677, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI295821200, Length=310, Percent_Identity=41.9354838709677, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI4557415, Length=310, Percent_Identity=41.9354838709677, Blast_Score=245, Evalue=4e-65,
Organism=Homo sapiens, GI11345492, Length=312, Percent_Identity=25.3205128205128, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788754, Length=317, Percent_Identity=51.7350157728707, Blast_Score=294, Evalue=4e-81,
Organism=Escherichia coli, GI2367138, Length=301, Percent_Identity=40.1993355481728, Blast_Score=231, Evalue=4e-62,
Organism=Escherichia coli, GI1790207, Length=236, Percent_Identity=30.0847457627119, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17535051, Length=300, Percent_Identity=51.6666666666667, Blast_Score=306, Evalue=1e-83,
Organism=Caenorhabditis elegans, GI115535073, Length=307, Percent_Identity=51.1400651465798, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI17562970, Length=300, Percent_Identity=48.6666666666667, Blast_Score=285, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI32566674, Length=295, Percent_Identity=49.4915254237288, Blast_Score=284, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17561720, Length=300, Percent_Identity=45.3333333333333, Blast_Score=243, Evalue=8e-65,
Organism=Caenorhabditis elegans, GI32566672, Length=212, Percent_Identity=47.1698113207547, Blast_Score=204, Evalue=5e-53,
Organism=Caenorhabditis elegans, GI17534315, Length=314, Percent_Identity=37.2611464968153, Blast_Score=187, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI25147552, Length=312, Percent_Identity=35.8974358974359, Blast_Score=175, Evalue=3e-44,
Organism=Caenorhabditis elegans, GI17561716, Length=118, Percent_Identity=40.6779661016949, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI71996324, Length=326, Percent_Identity=23.9263803680982, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321594, Length=318, Percent_Identity=39.937106918239, Blast_Score=217, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321449, Length=328, Percent_Identity=33.2317073170732, Blast_Score=138, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24643623, Length=311, Percent_Identity=38.5852090032154, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI20129101, Length=311, Percent_Identity=38.5852090032154, Blast_Score=206, Evalue=2e-53,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 33599; Mature: 33599

Theoretical pI: Translated: 5.58; Mature: 5.58

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIYKNITELIGNTPLLELEKLKKENKLKANIIAKVEYFNPANSVKDRIAFSMIEEAEK
CHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHH
EGLIDKDTVIIEPTSGNTGVGLAFVAAAKGYKLILTMPETMSMERRLILKAYGAELVLTP
CCCCCCCEEEEEECCCCCCCHHHEEECCCCCEEEEECCHHHHHHHHHHHEECCCEEEEEC
GKDGMKGAIEKATELSKEYKNSFVPQQFENKFNPAMHKRTTAVEIWNDTDGEVDIFIAGV
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHCCCEEEEEECCCCCCEEEEEEEE
GTGGTITGVGEYLKEKNKDIKIIAVEPEDSPVLSGGNPGPHKIQGIGAGFVPSTLNTEVL
CCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCEEEECCCCCCCCCCCHHHH
DEIFKVSNEKAFEVTKSIAKTEGLLVGISSGAAIYAAMKIAERQENQGKNIVVLLPDTGQ
HHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCH
RYLSTGVFE
HHHHCCCCC
>Mature Secondary Structure
MKKIYKNITELIGNTPLLELEKLKKENKLKANIIAKVEYFNPANSVKDRIAFSMIEEAEK
CHHHHHHHHHHHCCCCCHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHH
EGLIDKDTVIIEPTSGNTGVGLAFVAAAKGYKLILTMPETMSMERRLILKAYGAELVLTP
CCCCCCCEEEEEECCCCCCCHHHEEECCCCCEEEEECCHHHHHHHHHHHEECCCEEEEEC
GKDGMKGAIEKATELSKEYKNSFVPQQFENKFNPAMHKRTTAVEIWNDTDGEVDIFIAGV
CCCCHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHHHCCCEEEEEECCCCCCEEEEEEEE
GTGGTITGVGEYLKEKNKDIKIIAVEPEDSPVLSGGNPGPHKIQGIGAGFVPSTLNTEVL
CCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCCCEEEECCCCCCCCCCCHHHH
DEIFKVSNEKAFEVTKSIAKTEGLLVGISSGAAIYAAMKIAERQENQGKNIVVLLPDTGQ
HHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEECCCCH
RYLSTGVFE
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]