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The map label for this gene is psuK [H]

Identifier: 148379154

GI number: 148379154

Start: 1282835

End: 1283932

Strand: Direct

Name: psuK [H]

Synonym: CBO1168

Alternate gene names: 148379154

Gene position: 1282835-1283932 (Clockwise)

Preceding gene: 148379152

Following gene: 148379155

Centisome position: 33.0

GC content: 29.42

Gene sequence:

>1098_bases
ATGACAAATAGAGAAAAAGAAGTTTTAGAAGTAATAAAAGAAAATCCAATGATATCCCAAAAGGACTTAGCAGAGAAACT
AGGTATAACAAGATCTTCCGCAGCAGTGCATATAACTAATCTTTTGAAAAAAGGATATCTTTTAGGAAAAGGTTATATAG
TTTCAAAGGATGAAGAGTATGTTTCTATAATTGGTGGAGCAAATATGGATATACAAGGTTTTCCCAATGATAAATTAATA
TACAAAGATTCTAATCCAGGCAAAAGTAAAATATCATTAGGGGGAGTAGGCAGAAACATAGGAGAGAATTTAACTAAATT
AGGTATAAATACAAAACTTATAACTGCTCTAGGAGAAGATATATATGGAAACAAGATATTAGAAGAAGCAAAAACTATAG
GCATGGATATGGAACATTCTATAATAATGCGAGAAGATACAACTTCGACTTATCTTTCAATTCTAGATGAAACAGGAGAT
ATGATGGTTGCTATAGCTCACATGGATATATTTGATAAAATGCCTTTAGATTTTATTAAAAGTAAAAAAATTGTTATAGA
AAACTCAGGTGTATGTATAATTGATACCAATATACCACAGGAAATTATAGAATATATAGTTAACAATCATCAAAATGTAA
AATTCTTTTTAGATACAGTATCTACTACTAAGGCTAAAAAGGTAAAAAATATTATAGGAAAGTTCCATACTATTAAATTA
AATAGGATTGAGGCTGAAATATTGTCTGGAATTCCTATAAAAAAGGAAGAGGATCTAGAAAGATCCGCTGAGTTTTTTTT
AGAAAAGGGAGTTAAAAGAGTGTTTATAACTTTAGGTGAAGAAGGGGTTTATTATAATGATAGAAAAAATAAAAATAAAA
TACCTACTCCTAAGGTTAGAGTTATAAACGCAACAGGAGCAGGGGATGCTTTTATGGCAGCTATGGTCTATTGTTATTTA
AAAGATTTTTCTGTAGAAGAAACTATTAAGTTTTCAATGACAGCTTCCATACTAGCCTTATCCCATGAAGATACTATAAA
TCCAAATATGTCAGTTTGGAGCATAAATAATAAGATGAAGGAGATAGGAATATGTTAG

Upstream 100 bases:

>100_bases
ATAAATATTGAAAAGATAAAAAATATATGATATTATTTACTTAAACAAATGCTTATCGCATAAGCATTTGTTTAAGTAAA
TGGTTTTTGAGGGGATAAAT

Downstream 100 bases:

>100_bases
AAAAATATTTAGAAATTAGTAAAGAAGTATCAGAAGCCTTAAAGGAAAATAAACCTGTAGTAGCTTTAGAATCTACTATA
ATATCTCATGGGATGCCATA

Product: kinase, PfkB family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MTNREKEVLEVIKENPMISQKDLAEKLGITRSSAAVHITNLLKKGYLLGKGYIVSKDEEYVSIIGGANMDIQGFPNDKLI
YKDSNPGKSKISLGGVGRNIGENLTKLGINTKLITALGEDIYGNKILEEAKTIGMDMEHSIIMREDTTSTYLSILDETGD
MMVAIAHMDIFDKMPLDFIKSKKIVIENSGVCIIDTNIPQEIIEYIVNNHQNVKFFLDTVSTTKAKKVKNIIGKFHTIKL
NRIEAEILSGIPIKKEEDLERSAEFFLEKGVKRVFITLGEEGVYYNDRKNKNKIPTPKVRVINATGAGDAFMAAMVYCYL
KDFSVEETIKFSMTASILALSHEDTINPNMSVWSINNKMKEIGIC

Sequences:

>Translated_365_residues
MTNREKEVLEVIKENPMISQKDLAEKLGITRSSAAVHITNLLKKGYLLGKGYIVSKDEEYVSIIGGANMDIQGFPNDKLI
YKDSNPGKSKISLGGVGRNIGENLTKLGINTKLITALGEDIYGNKILEEAKTIGMDMEHSIIMREDTTSTYLSILDETGD
MMVAIAHMDIFDKMPLDFIKSKKIVIENSGVCIIDTNIPQEIIEYIVNNHQNVKFFLDTVSTTKAKKVKNIIGKFHTIKL
NRIEAEILSGIPIKKEEDLERSAEFFLEKGVKRVFITLGEEGVYYNDRKNKNKIPTPKVRVINATGAGDAFMAAMVYCYL
KDFSVEETIKFSMTASILALSHEDTINPNMSVWSINNKMKEIGIC
>Mature_364_residues
TNREKEVLEVIKENPMISQKDLAEKLGITRSSAAVHITNLLKKGYLLGKGYIVSKDEEYVSIIGGANMDIQGFPNDKLIY
KDSNPGKSKISLGGVGRNIGENLTKLGINTKLITALGEDIYGNKILEEAKTIGMDMEHSIIMREDTTSTYLSILDETGDM
MVAIAHMDIFDKMPLDFIKSKKIVIENSGVCIIDTNIPQEIIEYIVNNHQNVKFFLDTVSTTKAKKVKNIIGKFHTIKLN
RIEAEILSGIPIKKEEDLERSAEFFLEKGVKRVFITLGEEGVYYNDRKNKNKIPTPKVRVINATGAGDAFMAAMVYCYLK
DFSVEETIKFSMTASILALSHEDTINPNMSVWSINNKMKEIGIC

Specific function: Catalyzes the phosphorylation of pseudouridine to pseudouridine 5'-phosphate (PsiMP) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Homo sapiens, GI11545855, Length=253, Percent_Identity=26.4822134387352, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1788491, Length=298, Percent_Identity=39.9328859060403, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1788484, Length=353, Percent_Identity=32.5779036827195, Blast_Score=235, Evalue=3e-63,
Organism=Escherichia coli, GI1790193, Length=267, Percent_Identity=22.4719101123595, Blast_Score=87, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24580669, Length=299, Percent_Identity=24.0802675585284, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: =2.7.1.83 [H]

Molecular weight: Translated: 40817; Mature: 40686

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNREKEVLEVIKENPMISQKDLAEKLGITRSSAAVHITNLLKKGYLLGKGYIVSKDEEY
CCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCEEEECCEEEECCCCE
VSIIGGANMDIQGFPNDKLIYKDSNPGKSKISLGGVGRNIGENLTKLGINTKLITALGED
EEEECCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHCCC
IYGNKILEEAKTIGMDMEHSIIMREDTTSTYLSILDETGDMMVAIAHMDIFDKMPLDFIK
HHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEEHHHHHCCCHHHHC
SKKIVIENSGVCIIDTNIPQEIIEYIVNNHQNVKFFLDTVSTTKAKKVKNIIGKFHTIKL
CCEEEEECCCEEEEECCCHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHCCEEEEEE
NRIEAEILSGIPIKKEEDLERSAEFFLEKGVKRVFITLGEEGVYYNDRKNKNKIPTPKVR
EHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCCCCCCCCEE
VINATGAGDAFMAAMVYCYLKDFSVEETIKFSMTASILALSHEDTINPNMSVWSINNKMK
EEEECCCCHHHHHHHHHHHHHCCCHHHHHHHEEEEEEEEECCCCCCCCCCEEEEECCHHH
EIGIC
HHCCC
>Mature Secondary Structure 
TNREKEVLEVIKENPMISQKDLAEKLGITRSSAAVHITNLLKKGYLLGKGYIVSKDEEY
CCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHCCEEEECCEEEECCCCE
VSIIGGANMDIQGFPNDKLIYKDSNPGKSKISLGGVGRNIGENLTKLGINTKLITALGED
EEEECCCCCCCCCCCCCEEEEECCCCCCCEEEECCCCCHHHHHHHHHCCCHHHHHHHCCC
IYGNKILEEAKTIGMDMEHSIIMREDTTSTYLSILDETGDMMVAIAHMDIFDKMPLDFIK
HHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHCCCCEEEEEEEHHHHHCCCHHHHC
SKKIVIENSGVCIIDTNIPQEIIEYIVNNHQNVKFFLDTVSTTKAKKVKNIIGKFHTIKL
CCEEEEECCCEEEEECCCHHHHHHHHHCCCCCEEEEEEEHHHHHHHHHHHHHCCEEEEEE
NRIEAEILSGIPIKKEEDLERSAEFFLEKGVKRVFITLGEEGVYYNDRKNKNKIPTPKVR
EHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCCCCCCCCEE
VINATGAGDAFMAAMVYCYLKDFSVEETIKFSMTASILALSHEDTINPNMSVWSINNKMK
EEEECCCCHHHHHHHHHHHHHCCCHHHHHHHEEEEEEEEECCCCCCCCCCEEEEECCHHH
EIGIC
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 3076173 [H]