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The map label for this gene is gldA [H]

Identifier: 148378968

GI number: 148378968

Start: 1076382

End: 1077512

Strand: Reverse

Name: gldA [H]

Synonym: CBO0976

Alternate gene names: 148378968

Gene position: 1077512-1076382 (Counterclockwise)

Preceding gene: 148378981

Following gene: 148378967

Centisome position: 27.72

GC content: 33.69

Gene sequence:

>1131_bases
ATGAGAAAAGCATTTATTTGTCCAACTAAATATGTTCAAGGTGAAGATGAATTATTAAACTTAGGATATTTTGTAAAGAG
TTTTGGGGAATCAGCTCTATTAATAGCTCACAAAGATGACATAAAACGTGTTAAAGATAAATTAGATTCAACTGCTAAAA
AATTCGGAATTACTTTTGTAGAAAGTTCATTCTGTGGAGAATGTTCAAGAGAAGAAGTTGCAAGATTACAAGAAATCGCA
AAAAATAATAAATGTGCATGCACTATAGGACTTGGTGGTGGTAAAGCTATAGATACAGCAAAATGCGTTGCTAAGGGTAA
TTCATTAATAATTGTTCCAACAATAGCAGCTACAGATGCTCCAACTAGCCACTCTGCTGTACTTTACACACCAGACGGTG
CATTTGATGATTACGCTTACTTCTGGCAAAGTCCAACTGTAATTTTAATAGATACTACAGTTATTGCAAATGCTCCAACA
AGATTCTTAGTTTCAGGAATGGGTGATGCATTATCAACATATTTCGAAGCAAGAGCAACTTCAAATTCTTTTTCAAATGT
TAATGCAGGTTTACCTTGCGGAGTTAGAGAAGGATTATGTCCACCAGCAAAAGGAACTAACACAGCATTAACACTTGCAA
AAGCTTGTTATAAAACATTACTAGAAGATGGTGTAAAAGCAAAAATAGCATCAGATTGTAATTTAGTAACACAAGCATTA
GAAAACATAATAGAAACAAATATTCTTTTATCAGGACTAGGTTTTGAAAGTGCTGGTTTAGCAGCTGCTCATGCAATCCA
TGATGGATTAACAATACTAGAAGGAACTCACAAATACTTCCATGGAGAAAAAGTTGCCTTCGGAACAATTGCCCAATTAG
TTCTTGAAAATGCACCAAAAGAAGAAATTGATGAAGTAATAAACTTCTGCCTAGAAATTGGACTTCCAGTTTGCTTAGAA
GATATTGGTGTAGACAGCATTACTGATGAAGAACTTATGGAAGTAGCTAAAAAATCTTGTATAGAAGAAGAATCAATACA
CTCTATGCCATTTAAGGTAACTCCAGAAGCAGTAGCAGCAGCAATAATAACAGCAGATACTTTAGGTAAAAAATATAAAT
CAAATAAATAG

Upstream 100 bases:

>100_bases
TTTTTTGTCATGGAAATCCAATGCATATTTATGTAAACGATATTACAATAGAGACAAGCTAAAAAATAAAAATATTTTTA
CAAATTAAGGGGAGATTATA

Downstream 100 bases:

>100_bases
AGGTGTAAACATGAAAAAGATAATAAATAGACCTGAGACTGTAGTTATGGAGATGTGTAATGGAATAGCTATGGCACATC
CTGAACTAGAGTTTGTTAGA

Product: glycerol dehydrogenase

Products: NA

Alternate protein names: GDH; GLDH [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MRKAFICPTKYVQGEDELLNLGYFVKSFGESALLIAHKDDIKRVKDKLDSTAKKFGITFVESSFCGECSREEVARLQEIA
KNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDAPTSHSAVLYTPDGAFDDYAYFWQSPTVILIDTTVIANAPT
RFLVSGMGDALSTYFEARATSNSFSNVNAGLPCGVREGLCPPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL
ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPKEEIDEVINFCLEIGLPVCLE
DIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAAAIITADTLGKKYKSNK

Sequences:

>Translated_376_residues
MRKAFICPTKYVQGEDELLNLGYFVKSFGESALLIAHKDDIKRVKDKLDSTAKKFGITFVESSFCGECSREEVARLQEIA
KNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDAPTSHSAVLYTPDGAFDDYAYFWQSPTVILIDTTVIANAPT
RFLVSGMGDALSTYFEARATSNSFSNVNAGLPCGVREGLCPPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL
ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPKEEIDEVINFCLEIGLPVCLE
DIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAAAIITADTLGKKYKSNK
>Mature_376_residues
MRKAFICPTKYVQGEDELLNLGYFVKSFGESALLIAHKDDIKRVKDKLDSTAKKFGITFVESSFCGECSREEVARLQEIA
KNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDAPTSHSAVLYTPDGAFDDYAYFWQSPTVILIDTTVIANAPT
RFLVSGMGDALSTYFEARATSNSFSNVNAGLPCGVREGLCPPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL
ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPKEEIDEVINFCLEIGLPVCLE
DIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAAAIITADTLGKKYKSNK

Specific function: Catalyzes the NAD-dependent oxidation of glycerol to dihydroxyacetone (glycerone). Allows microorganisms to utilize glycerol as a source of carbon under anaerobic conditions. Exhibits a rather broad substrate specificity since it can also oxidize 1,2-prop

COG id: COG0371

COG function: function code C; Glycerol dehydrogenase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI87082352, Length=374, Percent_Identity=43.5828877005348, Blast_Score=317, Evalue=8e-88,
Organism=Escherichia coli, GI1786815, Length=305, Percent_Identity=30.1639344262295, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211
- InterPro:   IPR016205 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: =1.1.1.6 [H]

Molecular weight: Translated: 40221; Mature: 40221

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKAFICPTKYVQGEDELLNLGYFVKSFGESALLIAHKDDIKRVKDKLDSTAKKFGITFV
CCCCEECCHHHCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCEEE
ESSFCGECSREEVARLQEIAKNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDA
ECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEEEEECCC
PTSHSAVLYTPDGAFDDYAYFWQSPTVILIDTTVIANAPTRFLVSGMGDALSTYFEARAT
CCCCCEEEECCCCCCCCHHHHHCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHCC
SNSFSNVNAGLPCGVREGLCPPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPK
HHHHHHHHHEECCCCCCCCHHHHHHHHCCCCEEECCHHHHCCCCHHHHHHHHHHHCCCCH
EEIDEVINFCLEIGLPVCLEDIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAA
HHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCEECHHHHHH
AIITADTLGKKYKSNK
HHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRKAFICPTKYVQGEDELLNLGYFVKSFGESALLIAHKDDIKRVKDKLDSTAKKFGITFV
CCCCEECCHHHCCCCHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCEEE
ESSFCGECSREEVARLQEIAKNNKCACTIGLGGGKAIDTAKCVAKGNSLIIVPTIAATDA
ECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCCEEEEEEEEECCC
PTSHSAVLYTPDGAFDDYAYFWQSPTVILIDTTVIANAPTRFLVSGMGDALSTYFEARAT
CCCCCEEEECCCCCCCCHHHHHCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHCC
SNSFSNVNAGLPCGVREGLCPPAKGTNTALTLAKACYKTLLEDGVKAKIASDCNLVTQAL
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHH
ENIIETNILLSGLGFESAGLAAAHAIHDGLTILEGTHKYFHGEKVAFGTIAQLVLENAPK
HHHHHHHHHEECCCCCCCCHHHHHHHHCCCCEEECCHHHHCCCCHHHHHHHHHHHCCCCH
EEIDEVINFCLEIGLPVCLEDIGVDSITDEELMEVAKKSCIEEESIHSMPFKVTPEAVAA
HHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHCCCCEECHHHHHH
AIITADTLGKKYKSNK
HHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA