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The map label for this gene is def [H]

Identifier: 148378868

GI number: 148378868

Start: 978820

End: 979320

Strand: Reverse

Name: def [H]

Synonym: CBO0876

Alternate gene names: 148378868

Gene position: 979320-978820 (Counterclockwise)

Preceding gene: 148378870

Following gene: 148378867

Centisome position: 25.2

GC content: 33.93

Gene sequence:

>501_bases
ATGTCAACAAAACCAATCCTACTATTAGGTGATGAAATACTTTACAGAATTTCTGAGGAGATACAAAAATCAAATTTAGA
TAAAGCTAAGCAAGTAATCAATGACTTACATGATACGATTGTTTCTTTTAAAAATCGATATGGATTTGGTAGAGCCATTG
CCGCACCACAGATTGGTGAATCTGTCAGAATAATATACATGCATTTAGGCGATAAAACTCATTATTTCATTAATCCAATT
CTTGAATTTATTGGAGAGGATACCTTTCACCTCTGGGATGATTGCATGAGTTTCCCAGGTCTTGAAGTCTATGTAGAAAG
GCATAAGAAGTGCAAAGTAACTTATAAAAATTTAAGCTGGGAAGATTGTGAAATGTATCTCGAAGGTGATCTATCAGAAC
TGTTTCAGCACGAGTATGATCATTTAGACGGAATTTTGGCTGTACAACGTGCAAAGGATTTAAAATCATTCCGCATAAAT
AAAAATAAGAGCGGCTATTAA

Upstream 100 bases:

>100_bases
TGAACAGGAGCTGTCGGTGTGGGTTGCTTAGCTTAATGACAAAATCTCAGTTGTTTAGTATCTATTTATAATAATTTAGT
CCAAAAAGGGAGGTTACATT

Downstream 100 bases:

>100_bases
ATTGCAATAAACGCACTAATAATGCCTGCTCCTTCACTCTCTTTCTATCATTTTAATTCCAATACAATACTAGATTTAAC
TCTACCACTATTCTATACGA

Product: putative peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 166; Mature: 165

Protein sequence:

>166_residues
MSTKPILLLGDEILYRISEEIQKSNLDKAKQVINDLHDTIVSFKNRYGFGRAIAAPQIGESVRIIYMHLGDKTHYFINPI
LEFIGEDTFHLWDDCMSFPGLEVYVERHKKCKVTYKNLSWEDCEMYLEGDLSELFQHEYDHLDGILAVQRAKDLKSFRIN
KNKSGY

Sequences:

>Translated_166_residues
MSTKPILLLGDEILYRISEEIQKSNLDKAKQVINDLHDTIVSFKNRYGFGRAIAAPQIGESVRIIYMHLGDKTHYFINPI
LEFIGEDTFHLWDDCMSFPGLEVYVERHKKCKVTYKNLSWEDCEMYLEGDLSELFQHEYDHLDGILAVQRAKDLKSFRIN
KNKSGY
>Mature_165_residues
STKPILLLGDEILYRISEEIQKSNLDKAKQVINDLHDTIVSFKNRYGFGRAIAAPQIGESVRIIYMHLGDKTHYFINPIL
EFIGEDTFHLWDDCMSFPGLEVYVERHKKCKVTYKNLSWEDCEMYLEGDLSELFQHEYDHLDGILAVQRAKDLKSFRINK
NKSGY

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Drosophila melanogaster, GI24645728, Length=179, Percent_Identity=30.7262569832402, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19409; Mature: 19278

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKPILLLGDEILYRISEEIQKSNLDKAKQVINDLHDTIVSFKNRYGFGRAIAAPQIGE
CCCCCEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SVRIIYMHLGDKTHYFINPILEFIGEDTFHLWDDCMSFPGLEVYVERHKKCKVTYKNLSW
CEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCCH
EDCEMYLEGDLSELFQHEYDHLDGILAVQRAKDLKSFRINKNKSGY
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
STKPILLLGDEILYRISEEIQKSNLDKAKQVINDLHDTIVSFKNRYGFGRAIAAPQIGE
CCCCEEEECHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
SVRIIYMHLGDKTHYFINPILEFIGEDTFHLWDDCMSFPGLEVYVERHKKCKVTYKNLSW
CEEEEEEEECCCCHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHEEEECCCCH
EDCEMYLEGDLSELFQHEYDHLDGILAVQRAKDLKSFRINKNKSGY
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA