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The map label for this gene is metG [H]

Identifier: 148361194

GI number: 148361194

Start: 3429778

End: 3431790

Strand: Reverse

Name: metG [H]

Synonym: LPC_3168

Alternate gene names: 148361194

Gene position: 3431790-3429778 (Counterclockwise)

Preceding gene: 148361196

Following gene: 148361193

Centisome position: 95.95

GC content: 41.43

Gene sequence:

>2013_bases
ATGACTAGTGAACGAAAAATGTTAGTGACCAGTGCCCTGCCTTATGCTAATGGGCATTTGCATCTGGGACATTTGGTTGA
GCATATTCAAACAGATATTTGGGTCCGTACTCACAAAATGTTGGGTATTCAATGTATTAGCGTTTGTGGGGATGACGCTC
ATGGAACACCCATTATGCTGAAAGCAGAGCAGCTGGGAATTACTCCGGAAGCTTTAACCGCCGAAATTAAGTTAAGTCAT
GAAAAGGATTTTAAAGCCTTCGCCATTGATTATGATTATTACCACACCACACATTCACCTGAAAATCAGGCATTGGCGAC
TACCATATTTGAACGTTTGCAGGCAGGCGGTGATATAGTCAAGAAAACAATTCGCCAGTTTTATGATCCCGTAAAGCAAA
TGTTTTTGCCGGATAGGTATGTGAAGGGAACTTGCCCCAAATGTGCTGCTGTCGATCAGTATGGCGATAATTGCGAGGTT
TGTGGAGCCACTTATTCGCCGACGGATTTGATTAACCCTGTTTCTGTTATATCGGGAGCCAGCCCAGTAGAAATGGAATC
AGAGCATTATTTTTTTGATTTGCCGCGTTACGAAGAGTTACTTAAGGATTGGACAAGAAAGGGGCATTTACAAACCGAAG
TAACGAATAAACTCAGCGAATGGTTTGAGGCGGGTTTAAAGCAATGGGATATTTCTCGCGATGCTCCCTATTTTGGCTTC
CCTATCCCCGGTGTTCCGGATAAATACTTTTACGTTTGGTTAGATGCTCCCATAGGCTATATGGCAAGCTTTAAAAAGTA
TTGCGATGAGCGTGGCGTTTCTTTTGATGAGTTTTGGGATAAGGCTTCAAAAACAGAATTATATCATTTTGTAGGCAAGG
ATATTGTGTATTTTCATGCTTTGTTCTGGCCAGCTATGTTAGCTGCCAGCGGTTTCAGAACACCTACTGCCGTGTATACC
CATGGGTTTTTAACGGTTGAGGGGCAGAAAATGTCCAAATCGCGCGGTACTTTTATAGAAGCACGCGCTTATCTGGCTCA
CTTGCATCCTGAATATCTGCGTTATTATTTCGCTGCCAAATTGAATGGTCGAGTAGACGATTTGGATTTGAATTTTGACG
ATTTTGTCAATAGGGTGAATGCGGATCTGGTCGGGAAGGTAGTGAATATTGCAAGCCGGTGTGCGGGATTTATTAATAAG
CGCTTTGATAATCGGTTGAGCTCAGAGTTGATTAATCAAAAATTATACAACGATTTGCTGAGTGCAAGAGAATTCGTGAT
TGACGCATTTGTTTCAAGAGACTATGCGCGAGCAATAAGACAAATCATGGATTGTGCTGACAAAGTCAATCAATACATCG
ATGCCAATAAACCTTGGGTATTGGCTAAAGATGAAAGTAAACTAAATGAAGTTCATGCCATATGCACAATGGGAATCAAT
TTATTCCGCATTTTAATAACCTATTTAAAACCAGTCTTGCCAATGATGGCCAAAGCTTCAGAAGAATTTTTAAATTCTGA
GCCTTTACACTGGGGTTCTATTGATAAGCCTTTGCTAAACCATCGTATCAATACTTTTAAGCCTTTGATGGTCAGAGTAG
AAAAAGAAAAAATTGAAGCGATGTTGGTTCAAAGCAAGGAATCTCTGATGACTACCCCAATAAAAGAAAACACGCCAGTT
GAGGACTCTAACTTAATCAGTATTGAGGATTTTGCCAAAGTCGATTTACGGATTGCAAAAATTGTCAATGCAGAACCTGT
GGAAGGCGCAGATAAACTAATGCGCTTGATTTTAGACTTGGGTGATGCTCAAAAGCAGGTTTTTGCCGGCATAAAAAAGG
CTTATGATGCTGAAGAGTTGATTGGGCGTTTGACTGTAATGGTTGCTAATTTGGAGCCCCGCACTATGCGTTTCGGTGTT
TCTGAAGGCATGGTATTGGCGGCAGGGGATGGACAGGGAATTTATTTATTACAGCCTGATGCTGGAGCATTTCCTGGCAT
GAAGGTGAAGTAA

Upstream 100 bases:

>100_bases
TAAATAACTACCAATAAAAACCTGTTTTCTATTGCAGCATTTCCATTGTCTTTGCGTTATAATTAGTCGCTTTATTATAG
GTGATGAGTATTCTATAACT

Downstream 100 bases:

>100_bases
TGGCCTCTGTTAAAGAAATTGATGCCCTATTGCCGCAAACCCAGTGTGGGGAATGCGGATATGCAGGTTGTTTGCCTTAT
GCTGAAGCGCTTGCCCAAGG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 670; Mature: 669

Protein sequence:

>670_residues
MTSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSH
EKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEV
CGATYSPTDLINPVSVISGASPVEMESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGF
PIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYT
HGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINK
RFDNRLSSELINQKLYNDLLSAREFVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGIN
LFRILITYLKPVLPMMAKASEEFLNSEPLHWGSIDKPLLNHRINTFKPLMVRVEKEKIEAMLVQSKESLMTTPIKENTPV
EDSNLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDLGDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGV
SEGMVLAAGDGQGIYLLQPDAGAFPGMKVK

Sequences:

>Translated_670_residues
MTSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSH
EKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEV
CGATYSPTDLINPVSVISGASPVEMESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGF
PIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYT
HGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINK
RFDNRLSSELINQKLYNDLLSAREFVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGIN
LFRILITYLKPVLPMMAKASEEFLNSEPLHWGSIDKPLLNHRINTFKPLMVRVEKEKIEAMLVQSKESLMTTPIKENTPV
EDSNLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDLGDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGV
SEGMVLAAGDGQGIYLLQPDAGAFPGMKVK
>Mature_669_residues
TSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIMLKAEQLGITPEALTAEIKLSHE
KDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIVKKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEVC
GATYSPTDLINPVSVISGASPVEMESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGFP
IPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKASKTELYHFVGKDIVYFHALFWPAMLAASGFRTPTAVYTH
GFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAKLNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINKR
FDNRLSSELINQKLYNDLLSAREFVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGINL
FRILITYLKPVLPMMAKASEEFLNSEPLHWGSIDKPLLNHRINTFKPLMVRVEKEKIEAMLVQSKESLMTTPIKENTPVE
DSNLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDLGDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGVS
EGMVLAAGDGQGIYLLQPDAGAFPGMKVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=562, Percent_Identity=27.2241992882562, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI24308436, Length=523, Percent_Identity=23.7093690248566, Blast_Score=142, Evalue=1e-33,
Organism=Escherichia coli, GI1788432, Length=673, Percent_Identity=59.8811292719168, Blast_Score=862, Evalue=0.0,
Organism=Escherichia coli, GI1789455, Length=106, Percent_Identity=34.9056603773585, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17540794, Length=680, Percent_Identity=27.3529411764706, Blast_Score=217, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI212645096, Length=511, Percent_Identity=23.679060665362, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI71992781, Length=405, Percent_Identity=23.9506172839506, Blast_Score=121, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321704, Length=560, Percent_Identity=29.8214285714286, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6321610, Length=535, Percent_Identity=22.4299065420561, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24655311, Length=564, Percent_Identity=30.1418439716312, Blast_Score=243, Evalue=4e-64,
Organism=Drosophila melanogaster, GI21358483, Length=517, Percent_Identity=24.3713733075435, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 75928; Mature: 75797

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIML
CCCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
KAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIV
EHHHHCCCHHHHEEEEEECCCCCCEEEEEEECCEECCCCCCCHHHHHHHHHHHHCCHHHH
KKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEVCGATYSPTDLINPVSVISGA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHCCHHHHHCCC
SPVEMESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGF
CCCCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKASKTELYHFVGKDIVYFHA
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
LFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAK
HHHHHHHHHCCCCCCCEEEECCEEEEECCHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
LNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINKRFDNRLSSELINQKLYNDLL
CCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAREFVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHH
LFRILITYLKPVLPMMAKASEEFLNSEPLHWGSIDKPLLNHRINTFKPLMVRVEKEKIEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHEEHHHHHHH
MLVQSKESLMTTPIKENTPVEDSNLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDL
HHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
GDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGVSEGMVLAAGDGQGIYLLQPD
CHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCCCEEEECCCCEEEEECCCCEEEEECCC
AGAFPGMKVK
CCCCCCCCCC
>Mature Secondary Structure 
TSERKMLVTSALPYANGHLHLGHLVEHIQTDIWVRTHKMLGIQCISVCGDDAHGTPIML
CCCCCEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEE
KAEQLGITPEALTAEIKLSHEKDFKAFAIDYDYYHTTHSPENQALATTIFERLQAGGDIV
EHHHHCCCHHHHEEEEEECCCCCCEEEEEEECCEECCCCCCCHHHHHHHHHHHHCCHHHH
KKTIRQFYDPVKQMFLPDRYVKGTCPKCAAVDQYGDNCEVCGATYSPTDLINPVSVISGA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCHHHCCHHHHHCCC
SPVEMESEHYFFDLPRYEELLKDWTRKGHLQTEVTNKLSEWFEAGLKQWDISRDAPYFGF
CCCCCCCCCEEEECHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
PIPGVPDKYFYVWLDAPIGYMASFKKYCDERGVSFDEFWDKASKTELYHFVGKDIVYFHA
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
LFWPAMLAASGFRTPTAVYTHGFLTVEGQKMSKSRGTFIEARAYLAHLHPEYLRYYFAAK
HHHHHHHHHCCCCCCCEEEECCEEEEECCHHCCCCCCCHHHHHHHHHCCHHHHHHHHHHH
LNGRVDDLDLNFDDFVNRVNADLVGKVVNIASRCAGFINKRFDNRLSSELINQKLYNDLL
CCCCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAREFVIDAFVSRDYARAIRQIMDCADKVNQYIDANKPWVLAKDESKLNEVHAICTMGIN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHH
LFRILITYLKPVLPMMAKASEEFLNSEPLHWGSIDKPLLNHRINTFKPLMVRVEKEKIEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHEEHHHHHHH
MLVQSKESLMTTPIKENTPVEDSNLISIEDFAKVDLRIAKIVNAEPVEGADKLMRLILDL
HHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
GDAQKQVFAGIKKAYDAEELIGRLTVMVANLEPRTMRFGVSEGMVLAAGDGQGIYLLQPD
CHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCCCEEEECCCCEEEEECCCCEEEEECCC
AGAFPGMKVK
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA