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The map label for this gene is nth [H]

Identifier: 148361192

GI number: 148361192

Start: 3428536

End: 3429171

Strand: Reverse

Name: nth [H]

Synonym: LPC_3166

Alternate gene names: 148361192

Gene position: 3429171-3428536 (Counterclockwise)

Preceding gene: 148361193

Following gene: 148361191

Centisome position: 95.88

GC content: 41.98

Gene sequence:

>636_bases
ATGAATAAACAAAAATCACAAGAAATTTTCAAACGTTTTCAAGAACAAAACCCTCATCGTGCAACGGAGTTGCAATATAG
TACCCCCTTTGAGTTATTAATTGCCGTCATGCTTTCAGCCCAGGCAACAGACGTTAGTGTGAACAAAGCGACAGCCAAAC
TATTCCCGGTCGCCAATACCCCACAAGCCTTGCTGGATTTGGGATTAGATAGATTAAAAGATTATATTAAGTCCATAGGT
CTTTATAATAGTAAAGCACAAAATATCATGAAAACCTGTGCAATACTTATTGATCAGCATGAGGGACAAGTTCCTGGCCA
GAGAGAGGCGCTGGAAGCATTGCCTGGTGTTGGCCGAAAAACTGCAAATGTGGTTTTGAATACAGCATTTGGTCAGCCAA
CCATAGCGGTCGACACCCATATTTTCAGAGTGGCTAACCGGATTGGCATAGCGAAGGGTAATACACCGCTGGCGGTTGAG
CGGGAGTTATTAAAAAAAGTGCCCCGTGAGTTTTTACATGACGCTCATCACTGGTTAATATTACATGGCCGTTATGTCTG
TACAGCGCGTAAACCACATTGCAAGACTTGCATCATTGAAGATCTTTGCGAATACAAAGACAAGAATTTACTTTAA

Upstream 100 bases:

>100_bases
AAGAAACTTTCAGCCCATGCGCAAGATCATACTCAAGACGTACAGGCAAAACAAAATTATATTCTTGAGGCTTTGGCTCG
GGTGAAAGCAAAAAAGACAT

Downstream 100 bases:

>100_bases
GACAAAGCCATAATGAAGGAAAATCTAACGATAATGAACAATCTATGCCTCATAAAAATGACTGGCTGTTGTATTTCAAA
TTCATTCTATCAAATCATCC

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 211; Mature: 211

Protein sequence:

>211_residues
MNKQKSQEIFKRFQEQNPHRATELQYSTPFELLIAVMLSAQATDVSVNKATAKLFPVANTPQALLDLGLDRLKDYIKSIG
LYNSKAQNIMKTCAILIDQHEGQVPGQREALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRVANRIGIAKGNTPLAVE
RELLKKVPREFLHDAHHWLILHGRYVCTARKPHCKTCIIEDLCEYKDKNLL

Sequences:

>Translated_211_residues
MNKQKSQEIFKRFQEQNPHRATELQYSTPFELLIAVMLSAQATDVSVNKATAKLFPVANTPQALLDLGLDRLKDYIKSIG
LYNSKAQNIMKTCAILIDQHEGQVPGQREALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRVANRIGIAKGNTPLAVE
RELLKKVPREFLHDAHHWLILHGRYVCTARKPHCKTCIIEDLCEYKDKNLL
>Mature_211_residues
MNKQKSQEIFKRFQEQNPHRATELQYSTPFELLIAVMLSAQATDVSVNKATAKLFPVANTPQALLDLGLDRLKDYIKSIG
LYNSKAQNIMKTCAILIDQHEGQVPGQREALEALPGVGRKTANVVLNTAFGQPTIAVDTHIFRVANRIGIAKGNTPLAVE
RELLKKVPREFLHDAHHWLILHGRYVCTARKPHCKTCIIEDLCEYKDKNLL

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=33.3333333333333, Blast_Score=97, Evalue=1e-20,
Organism=Escherichia coli, GI1787920, Length=208, Percent_Identity=72.1153846153846, Blast_Score=313, Evalue=7e-87,
Organism=Caenorhabditis elegans, GI17554540, Length=175, Percent_Identity=34.2857142857143, Blast_Score=106, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6324530, Length=195, Percent_Identity=27.1794871794872, Blast_Score=80, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6319304, Length=174, Percent_Identity=28.735632183908, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI45550361, Length=191, Percent_Identity=30.3664921465969, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 23721; Mature: 23721

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQKSQEIFKRFQEQNPHRATELQYSTPFELLIAVMLSAQATDVSVNKATAKLFPVANT
CCCHHHHHHHHHHHHCCCCCHHEECCCCCHHHHHHHHHHCCCCCCCCCHHHHEEECCCCC
PQALLDLGLDRLKDYIKSIGLYNSKAQNIMKTCAILIDQHEGQVPGQREALEALPGVGRK
HHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGQPTIAVDTHIFRVANRIGIAKGNTPLAVERELLKKVPREFLHDAHHWLI
HHHHEEECCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
LHGRYVCTARKPHCKTCIIEDLCEYKDKNLL
EECEEEEECCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNKQKSQEIFKRFQEQNPHRATELQYSTPFELLIAVMLSAQATDVSVNKATAKLFPVANT
CCCHHHHHHHHHHHHCCCCCHHEECCCCCHHHHHHHHHHCCCCCCCCCHHHHEEECCCCC
PQALLDLGLDRLKDYIKSIGLYNSKAQNIMKTCAILIDQHEGQVPGQREALEALPGVGRK
HHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCC
TANVVLNTAFGQPTIAVDTHIFRVANRIGIAKGNTPLAVERELLKKVPREFLHDAHHWLI
HHHHEEECCCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEE
LHGRYVCTARKPHCKTCIIEDLCEYKDKNLL
EECEEEEECCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]