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The map label for this gene is rna [H]

Identifier: 148361159

GI number: 148361159

Start: 3392423

End: 3393403

Strand: Reverse

Name: rna [H]

Synonym: LPC_3133

Alternate gene names: 148361159

Gene position: 3393403-3392423 (Counterclockwise)

Preceding gene: 148361162

Following gene: 148361158

Centisome position: 94.88

GC content: 38.53

Gene sequence:

>981_bases
ATGATAAAACCAATCACCCTGCTTTTGCTTTTTTGTTCTTTCACTGTTAATGCCTCAATTCTCGTCAATGGTACTTTCGA
AGCAACAAAATCATGCCCTGCTTACCTTTCCAAGAATCGTAAATCAAATCCTGATAATTTAATGGTTGAGCCCAACCAGA
AATATCAATTAAAAGAAATTAATAAAACCAAACCGGATTGGTTGCGTATACAATTTTCAGACCATCAACCTAATCTGCGC
TGGGTGAGTGTTGATTGCGGGGTCATTGAATACACTGAGCGTCCCAAAGCGATCTGTGATCTTTCTCCCGGGATGGCTGA
TTCTTATGTGTTGGCTTTAAGTTCACAGCCGGGTTTTTGTGAAACTTATGGATATGAAATAGGCAAACCAGAGTGTTTGA
AATTAACAAAGACAGCTTATCAGGCCAATCATCTGATCCTGCATGGATTATGGCCTAATCAAGAAGCATGCGGCCATAAT
TATGGCTATTGTGGAATAAGCCCTCGAACAAACCATTGTGATTATTCTCCATTGGATTTGTCTTCTGATGTTGCTCAAAA
TTTAAAGAAACTTATGCCGAGTTACCATTATGGCAGTTGTCTGGAGCGCCATGAATGGAACAAACATGGGAGCTGTCAGG
ATTTAAGCACAGATGGTTATTTTAGTTTGGCCATGCGCTTAACTACAGAAGTTAATAATACAGAGTTCGGTCAATATCTT
ACCGCTCATCGTGGTGATACTGTAAAATTGACTACTTTGCGTGAATTGATTCAAAGATCTTTCGGGGAAAACAATACAGG
TAAAGTGACTTTAAGTTGCAAGAATGGGATCTTGGTGGATATTTATGTCCAATTACCTGCTCTCATACCTTTTAAAGAGT
CTCTAGAGTCTTTGGTTGATAAAGCCAGTCACTCACAACACCATGATTATTGTAATAGCAATGTCACCATTTCTGATTTT
AGTAATGAAAAATGGTTATAG

Upstream 100 bases:

>100_bases
AAAAAAATCCTTATTAATCAATTTCATGAAAAAATAATTGCTGGCATTCATATCCGACTAGGGTATAATGGGCTTACCAA
AGACAAGCCAGGATGTCGTA

Downstream 100 bases:

>100_bases
GTATTCAAGTGGAGGAGATGACTCGTTCACAAAACTTTCTTTAAAATAATATGGCATTGCTTCCTTATAGGAGACTGCCT
TGCACATTGTCAAAACTTCC

Product: T2 family transporter ribonuclease

Products: NA

Alternate protein names: RNase I; Enterobacter ribonuclease [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MIKPITLLLLFCSFTVNASILVNGTFEATKSCPAYLSKNRKSNPDNLMVEPNQKYQLKEINKTKPDWLRIQFSDHQPNLR
WVSVDCGVIEYTERPKAICDLSPGMADSYVLALSSQPGFCETYGYEIGKPECLKLTKTAYQANHLILHGLWPNQEACGHN
YGYCGISPRTNHCDYSPLDLSSDVAQNLKKLMPSYHYGSCLERHEWNKHGSCQDLSTDGYFSLAMRLTTEVNNTEFGQYL
TAHRGDTVKLTTLRELIQRSFGENNTGKVTLSCKNGILVDIYVQLPALIPFKESLESLVDKASHSQHHDYCNSNVTISDF
SNEKWL

Sequences:

>Translated_326_residues
MIKPITLLLLFCSFTVNASILVNGTFEATKSCPAYLSKNRKSNPDNLMVEPNQKYQLKEINKTKPDWLRIQFSDHQPNLR
WVSVDCGVIEYTERPKAICDLSPGMADSYVLALSSQPGFCETYGYEIGKPECLKLTKTAYQANHLILHGLWPNQEACGHN
YGYCGISPRTNHCDYSPLDLSSDVAQNLKKLMPSYHYGSCLERHEWNKHGSCQDLSTDGYFSLAMRLTTEVNNTEFGQYL
TAHRGDTVKLTTLRELIQRSFGENNTGKVTLSCKNGILVDIYVQLPALIPFKESLESLVDKASHSQHHDYCNSNVTISDF
SNEKWL
>Mature_326_residues
MIKPITLLLLFCSFTVNASILVNGTFEATKSCPAYLSKNRKSNPDNLMVEPNQKYQLKEINKTKPDWLRIQFSDHQPNLR
WVSVDCGVIEYTERPKAICDLSPGMADSYVLALSSQPGFCETYGYEIGKPECLKLTKTAYQANHLILHGLWPNQEACGHN
YGYCGISPRTNHCDYSPLDLSSDVAQNLKKLMPSYHYGSCLERHEWNKHGSCQDLSTDGYFSLAMRLTTEVNNTEFGQYL
TAHRGDTVKLTTLRELIQRSFGENNTGKVTLSCKNGILVDIYVQLPALIPFKESLESLVDKASHSQHHDYCNSNVTISDF
SNEKWL

Specific function: One of the few RNases that cleaves the phosphodiester bond between any two nucleotide. Shows a preference for cytidylic or guanylic acid [H]

COG id: COG3719

COG function: function code J; Ribonuclease I

Gene ontology:

Cell location: Periplasm. Cytoplasm. Note=RNase I (periplasmic) and RNase I* (cytoplasmic) appear to be isoforms apparently encoded by the same gene. The cytoplasmic form is less active towards natural polymer RNA [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase T2 family [H]

Homologues:

Organism=Escherichia coli, GI1786828, Length=206, Percent_Identity=32.0388349514563, Blast_Score=99, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001568
- InterPro:   IPR018188 [H]

Pfam domain/function: PF00445 Ribonuclease_T2 [H]

EC number: =3.1.27.6 [H]

Molecular weight: Translated: 36865; Mature: 36865

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS00530 RNASE_T2_1 ; PS00531 RNASE_T2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPITLLLLFCSFTVNASILVNGTFEATKSCPAYLSKNRKSNPDNLMVEPNQKYQLKEI
CCCHHHHHHHHHHHHCCEEEEEECCHHHHHCCHHHHHCCCCCCCCCEEECCCCCEEHHHC
NKTKPDWLRIQFSDHQPNLRWVSVDCGVIEYTERPKAICDLSPGMADSYVLALSSQPGFC
CCCCCCEEEEEECCCCCCEEEEEECCCEEEECCCCCEEEECCCCCCCCEEEEECCCCCCH
ETYGYEIGKPECLKLTKTAYQANHLILHGLWPNQEACGHNYGYCGISPRTNHCDYSPLDL
HHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHCCCCCCEEECCCCCCCCCCCCCCC
SSDVAQNLKKLMPSYHYGSCLERHEWNKHGSCQDLSTDGYFSLAMRLTTEVNNTEFGQYL
CHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHH
TAHRGDTVKLTTLRELIQRSFGENNTGKVTLSCKNGILVDIYVQLPALIPFKESLESLVD
HHCCCCEEEHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHH
KASHSQHHDYCNSNVTISDFSNEKWL
HHCCCCCHHHCCCCEEEEECCCCCCC
>Mature Secondary Structure
MIKPITLLLLFCSFTVNASILVNGTFEATKSCPAYLSKNRKSNPDNLMVEPNQKYQLKEI
CCCHHHHHHHHHHHHCCEEEEEECCHHHHHCCHHHHHCCCCCCCCCEEECCCCCEEHHHC
NKTKPDWLRIQFSDHQPNLRWVSVDCGVIEYTERPKAICDLSPGMADSYVLALSSQPGFC
CCCCCCEEEEEECCCCCCEEEEEECCCEEEECCCCCEEEECCCCCCCCEEEEECCCCCCH
ETYGYEIGKPECLKLTKTAYQANHLILHGLWPNQEACGHNYGYCGISPRTNHCDYSPLDL
HHCCCCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHCCCCCCEEECCCCCCCCCCCCCCC
SSDVAQNLKKLMPSYHYGSCLERHEWNKHGSCQDLSTDGYFSLAMRLTTEVNNTEFGQYL
CHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHH
TAHRGDTVKLTTLRELIQRSFGENNTGKVTLSCKNGILVDIYVQLPALIPFKESLESLVD
HHCCCCEEEHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEEEEECCCCCCHHHHHHHHHH
KASHSQHHDYCNSNVTISDFSNEKWL
HHCCCCCHHHCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2253883; 8905232; 9278503; 8188249 [H]