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The map label for this gene is dapF [H]

Identifier: 148361018

GI number: 148361018

Start: 461583

End: 462416

Strand: Reverse

Name: dapF [H]

Synonym: LPC_2978

Alternate gene names: 148361018

Gene position: 462416-461583 (Counterclockwise)

Preceding gene: 148361017

Following gene: 148361019

Centisome position: 12.93

GC content: 39.93

Gene sequence:

>834_bases
ATGGGAATAAAATTTACCAAAATGCATGGTTTAGGCAATGACTTCATTGTATTGGATGGAGTCAACCAATCTATTCAACT
CACTGTTGAGCAAATACAAAAACTAGCCAATCGCCATACCGGTATAGGATTTGACCAATGTTTACTAATAGAATCCAGTC
AAACTGAAGGAATTGATTTTAATTATCGAATCTTCAACGCGGATGGGCAAGAAGTAGGTCAATGTGGTAATGGGGCGCGA
TGCATAGCTCTTTTTGCCAGATATTATGGTTTAACAGCAAAAAATAAATTGACAGTAGCAACTAAAACTACGCTCATGGA
TTTAATCATTAATGAGGACAACAGCGTGAGTGTCAATATGGGCGTTCCCAGACTAGCCCCAGGTGAAATTCCTTTACTTG
CTGATCGCCAATCCCCAGAATATTCACTTGAATTAAATAACGGCAATACAGTGAATCTTCATGCGATAAGTGTAGGCAAT
CCTCATGCTGTATTGTTGGTTGAAAATATAGATACAGCCCCTGTAAACAGTTTGGGACAACAGATTAGTTTCCACCCGCA
ATTCCCAGAACAAGTCAACGTTGGATTTATGCAAATTGTTAATCATGAAAAAATCAATTTACGTGTCTACGAAAGGGGTT
GTGGTGAAACGATTGCTTGTGGTAGTGGTGCGGTTGCTGCGGCAGCAATAGCACGTTTATTTTATAACCTGTCAGACAAA
ATCACCGTTCATTTGCCAGGAGGAGATTTGTGTATCCAATGGCCTTGTCCCACTGCTCCCATTATATTAACAGGACCTGC
TGCTTTTGTGTATGAGGGCACATTACTTTCATAA

Upstream 100 bases:

>100_bases
CACCCTGACTTCTTTGTGTTTGTCAGCTTGTGGGCAAAAAGGACCTTTGTATTTGCCTGAACAAGAAAAGAAAGCAACAA
CCAATCAATAGAGACATCTT

Downstream 100 bases:

>100_bases
CAAAATTCACAAAATTAAAACAAGTATTGAATTTTTTTTTCAAACAGTTATTCTGAATTAATCCACAAATATAAAACAGG
ACTTAGGAAAAACGCTAAGG

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MGIKFTKMHGLGNDFIVLDGVNQSIQLTVEQIQKLANRHTGIGFDQCLLIESSQTEGIDFNYRIFNADGQEVGQCGNGAR
CIALFARYYGLTAKNKLTVATKTTLMDLIINEDNSVSVNMGVPRLAPGEIPLLADRQSPEYSLELNNGNTVNLHAISVGN
PHAVLLVENIDTAPVNSLGQQISFHPQFPEQVNVGFMQIVNHEKINLRVYERGCGETIACGSGAVAAAAIARLFYNLSDK
ITVHLPGGDLCIQWPCPTAPIILTGPAAFVYEGTLLS

Sequences:

>Translated_277_residues
MGIKFTKMHGLGNDFIVLDGVNQSIQLTVEQIQKLANRHTGIGFDQCLLIESSQTEGIDFNYRIFNADGQEVGQCGNGAR
CIALFARYYGLTAKNKLTVATKTTLMDLIINEDNSVSVNMGVPRLAPGEIPLLADRQSPEYSLELNNGNTVNLHAISVGN
PHAVLLVENIDTAPVNSLGQQISFHPQFPEQVNVGFMQIVNHEKINLRVYERGCGETIACGSGAVAAAAIARLFYNLSDK
ITVHLPGGDLCIQWPCPTAPIILTGPAAFVYEGTLLS
>Mature_276_residues
GIKFTKMHGLGNDFIVLDGVNQSIQLTVEQIQKLANRHTGIGFDQCLLIESSQTEGIDFNYRIFNADGQEVGQCGNGARC
IALFARYYGLTAKNKLTVATKTTLMDLIINEDNSVSVNMGVPRLAPGEIPLLADRQSPEYSLELNNGNTVNLHAISVGNP
HAVLLVENIDTAPVNSLGQQISFHPQFPEQVNVGFMQIVNHEKINLRVYERGCGETIACGSGAVAAAAIARLFYNLSDKI
TVHLPGGDLCIQWPCPTAPIILTGPAAFVYEGTLLS

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=271, Percent_Identity=48.7084870848708, Blast_Score=275, Evalue=3e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 29940; Mature: 29808

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGIKFTKMHGLGNDFIVLDGVNQSIQLTVEQIQKLANRHTGIGFDQCLLIESSQTEGIDF
CCEEEEEECCCCCCEEEECCCCCCEEEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEE
NYRIFNADGQEVGQCGNGARCIALFARYYGLTAKNKLTVATKTTLMDLIINEDNSVSVNM
EEEEECCCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHEEEEEEECCCCCEEEEC
GVPRLAPGEIPLLADRQSPEYSLELNNGNTVNLHAISVGNPHAVLLVENIDTAPVNSLGQ
CCCCCCCCCCCEEECCCCCCEEEEECCCCEEEEEEEEECCCEEEEEEECCCCCCHHHHHH
QISFHPQFPEQVNVGFMQIVNHEKINLRVYERGCGETIACGSGAVAAAAIARLFYNLSDK
CEECCCCCCHHHCCCEEEEECCCEEEEEEEECCCCCEEECCCCHHHHHHHHHHHHCCCCE
ITVHLPGGDLCIQWPCPTAPIILTGPAAFVYEGTLLS
EEEEECCCCEEEECCCCCCCEEEECCCEEEEECEECC
>Mature Secondary Structure 
GIKFTKMHGLGNDFIVLDGVNQSIQLTVEQIQKLANRHTGIGFDQCLLIESSQTEGIDF
CEEEEEECCCCCCEEEECCCCCCEEEEHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEE
NYRIFNADGQEVGQCGNGARCIALFARYYGLTAKNKLTVATKTTLMDLIINEDNSVSVNM
EEEEECCCCHHHHCCCCCHHHHHHHHHHHCCCCCCEEEEEEHHEEEEEEECCCCCEEEEC
GVPRLAPGEIPLLADRQSPEYSLELNNGNTVNLHAISVGNPHAVLLVENIDTAPVNSLGQ
CCCCCCCCCCCEEECCCCCCEEEEECCCCEEEEEEEEECCCEEEEEEECCCCCCHHHHHH
QISFHPQFPEQVNVGFMQIVNHEKINLRVYERGCGETIACGSGAVAAAAIARLFYNLSDK
CEECCCCCCHHHCCCEEEEECCCEEEEEEEECCCCCEEECCCCHHHHHHHHHHHHCCCCE
ITVHLPGGDLCIQWPCPTAPIILTGPAAFVYEGTLLS
EEEEECCCCEEEECCCCCCCEEEECCCEEEEECEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA