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The map label for this gene is cspD [H]

Identifier: 148360957

GI number: 148360957

Start: 517890

End: 518123

Strand: Reverse

Name: cspD [H]

Synonym: LPC_2917

Alternate gene names: 148360957

Gene position: 518123-517890 (Counterclockwise)

Preceding gene: 148360955

Following gene: 148360958

Centisome position: 14.49

GC content: 45.3

Gene sequence:

>234_bases
ATGGCTAGAGGCGAAGTCAAGTGGTTTAATAACGCCAAGGGTTGGGGTTTTATTATCCCGGAAGGTGGTGGTGAAGATAT
TTTTGTTCACTTCTCCTCAATTCACGGCACGGGATACAAAACGCTGGCTCCAGGACAGGCGGTCACCTATGATGTCGTTA
ATGGTGAAAGAGGTTTACATGCCTCTAATGTCGTTGCTCTTAGTGAAAACGCCGAACAAGAAGAAATAGCTTAA

Upstream 100 bases:

>100_bases
AAATAGGCTATTAACAATTGTCATTTAAGGAAGAATGCAATTGCTCAGCTGACCGCTTATGTCAGTCGACGATTGATAAG
CTTTAGCATAAGGAGGATTC

Downstream 100 bases:

>100_bases
CCCAATTAATGTGATAAACTATTAAGCGCGGCAGTATAACTAATATTTATGCTGCTGCGTCCATCAACTTGAGGTATTAT
GTCCTTCATTTTATAACTGG

Product: cold shock protein CspD

Products: NA

Alternate protein names: CSP-D [H]

Number of amino acids: Translated: 77; Mature: 76

Protein sequence:

>77_residues
MARGEVKWFNNAKGWGFIIPEGGGEDIFVHFSSIHGTGYKTLAPGQAVTYDVVNGERGLHASNVVALSENAEQEEIA

Sequences:

>Translated_77_residues
MARGEVKWFNNAKGWGFIIPEGGGEDIFVHFSSIHGTGYKTLAPGQAVTYDVVNGERGLHASNVVALSENAEQEEIA
>Mature_76_residues
ARGEVKWFNNAKGWGFIIPEGGGEDIFVHFSSIHGTGYKTLAPGQAVTYDVVNGERGLHASNVVALSENAEQEEIA

Specific function: Inhibits DNA replication at both initiation and elongation steps, most probably by binding to the opened, single- stranded regions at replication forks. Plays a regulatory role in chromosomal replication in nutrient-depleted cells [H]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=71, Percent_Identity=59.1549295774648, Blast_Score=91, Evalue=2e-20,
Organism=Escherichia coli, GI1786841, Length=65, Percent_Identity=56.9230769230769, Blast_Score=86, Evalue=4e-19,
Organism=Escherichia coli, GI1788126, Length=65, Percent_Identity=53.8461538461538, Blast_Score=82, Evalue=7e-18,
Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=51.5625, Blast_Score=73, Evalue=3e-15,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=48.4375, Blast_Score=71, Evalue=1e-14,
Organism=Escherichia coli, GI1787839, Length=62, Percent_Identity=51.6129032258064, Blast_Score=70, Evalue=2e-14,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=51.5625, Blast_Score=70, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24658520, Length=61, Percent_Identity=44.2622950819672, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 8276; Mature: 8145

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARGEVKWFNNAKGWGFIIPEGGGEDIFVHFSSIHGTGYKTLAPGQAVTYDVVNGERGLH
CCCCCEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCEEECCCCEEEEEEECCCCCCC
ASNVVALSENAEQEEIA
CCCEEEECCCCCCCCCC
>Mature Secondary Structure 
ARGEVKWFNNAKGWGFIIPEGGGEDIFVHFSSIHGTGYKTLAPGQAVTYDVVNGERGLH
CCCCEEECCCCCCEEEEEECCCCCEEEEEEECCCCCCCEEECCCCEEEEEEECCCCCCC
ASNVVALSENAEQEEIA
CCCEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]