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The map label for this gene is purH [H]

Identifier: 148360923

GI number: 148360923

Start: 553719

End: 555308

Strand: Reverse

Name: purH [H]

Synonym: LPC_2883

Alternate gene names: 148360923

Gene position: 555308-553719 (Counterclockwise)

Preceding gene: 148360922

Following gene: 148360924

Centisome position: 15.53

GC content: 40.19

Gene sequence:

>1590_bases
ATGGCGAAAAAACAAATTTACTCTTCTTTCAATCCACGTCGAGCACTGCTGAGTGTTTCTGATAAAAGAGGAATAAGAGA
ACTCGGTCAGGCCTTACATGACCATGGAGTGGAATTGATAGCCACAGGCAACACAGCTGCGATATTAAGAGAGCATAAAT
TACCAGTTACCGATGTCAGTGAGTGCACGGGATTTCCCGAAATAATGGATGGAAGAGTCAAAACCCTTCATCCAGCTATT
CATGCCGGTTTATTAGCCCGCGGAGAACAAGACAGCCCTGTTTTAAAACAACATGGAATAAAACCTATTGATTTACTTGT
TGTCAATTTATACCCCTTTGAACAAGTCATTAACCACCCGGACTGCGATTTTAATAAGGCCATTGAAAACATTGACATTG
GTGGACCAGCAATGGTGCGAGCAGCAGCTAAAAATCATGCCCATACTTATGTCATCGTTGATCCCAATGATTACCCAAAA
TTAATTCATTATTTACAAGATCAGAAAGCACCTTCCCATTGGAATTTTGCGTTAGCTAAAAAAGCATTTGCCCATACTGC
AGCCTACGATGCCGCAATTGCAAATTATTTAACTACTTTGGATAATGATTATGTGCCAACTGGATTTCCCGACATACTAA
CCTGCCAATTTAATAAAATTACTGATCTTCGTTATGGTGAAAATCCCCATCAGCAAGCTATTTTTTATGCTGATAAAAAT
TCACATCCAGGCTCTCTAGGTACAGCAGCCTTATTACAGGGAAAACAATTATCCTATAATAATATCCTTGATGCCGATGC
AGCTCTTGATTGTGTGAAATCATTTTCCAATGAAAAGTCGGTTTGCGTCATTGTAAAACATACCAACCCCTGTGGCATTG
CGCTATCCGACACATCACTTGATGCTTACTTAAAAGCCTTTCAAAGTGATCCGATATCCGCTTATGGTGGAATTATTGCT
TTCAATGGGACTCTGGATAGCGATACGGCCAAAGCAATATTAGAAAAGCAATTTGTTGAAGTGATTATTGCGCCTGATGC
CAATGAAGAAGCAAAAAAAATTCTGGCAGCCAAAGAGAACATTCGTGTTCTTCTTACCGGTTTTTGGCAACAAGGTACCA
ATTTTAGATTAAATATGAAGAAAGTCGATGGTGGTCTATTAGTACAAGAACACGATTCTCTTTCTCTGGAATCATGTGAG
TTACAAACCGTTACCCAAGCAAAACCCACTGATGAACAACTACAAAATTTAATGTTTGCTTGGTTAGCAGCCAAGCATGT
CAAATCCAATGCCATTGTATACGCTAATGATTTGGCCACTATAGGGATTGGCGGAGGACAAACCAGTCGAGTAATGAGTG
CTCGTATTGGTTTGTGGCAAGCAGAGCAAATGGGTTTTGATCCCCAAGGCGCTGTTATGGCCTCAGACGCATTTATCCCT
TTCCCTGATACTATTGAAATAGCTGCGAAAGCTGGTATATCCGCAATTATTCAACCAGGGGGTTCTATCAGAGATGAAAA
AATCATTTCTTGCGCCGACCAACACAACATAGCGATGATTTTTACAGGAGTGCGACATTTTAAACATTAA

Upstream 100 bases:

>100_bases
ATGATTCTGCATTCACTCATATTGCAACTGAATATTGTGAGGGATGGTCGTTGCTGGTTTTTACCAGCAAATAATAACTA
TATCACTAGGGTATATGTAA

Downstream 100 bases:

>100_bases
TGTCAAGCATGTATTAATTCCAAGCTTGACTAACTTGAACAAAGAGTCGTATATAACGTATCATCTTCTTTTTAATTGAA
GCTTTTTGGTTAGCATAAAT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 529; Mature: 528

Protein sequence:

>529_residues
MAKKQIYSSFNPRRALLSVSDKRGIRELGQALHDHGVELIATGNTAAILREHKLPVTDVSECTGFPEIMDGRVKTLHPAI
HAGLLARGEQDSPVLKQHGIKPIDLLVVNLYPFEQVINHPDCDFNKAIENIDIGGPAMVRAAAKNHAHTYVIVDPNDYPK
LIHYLQDQKAPSHWNFALAKKAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFNKITDLRYGENPHQQAIFYADKN
SHPGSLGTAALLQGKQLSYNNILDADAALDCVKSFSNEKSVCVIVKHTNPCGIALSDTSLDAYLKAFQSDPISAYGGIIA
FNGTLDSDTAKAILEKQFVEVIIAPDANEEAKKILAAKENIRVLLTGFWQQGTNFRLNMKKVDGGLLVQEHDSLSLESCE
LQTVTQAKPTDEQLQNLMFAWLAAKHVKSNAIVYANDLATIGIGGGQTSRVMSARIGLWQAEQMGFDPQGAVMASDAFIP
FPDTIEIAAKAGISAIIQPGGSIRDEKIISCADQHNIAMIFTGVRHFKH

Sequences:

>Translated_529_residues
MAKKQIYSSFNPRRALLSVSDKRGIRELGQALHDHGVELIATGNTAAILREHKLPVTDVSECTGFPEIMDGRVKTLHPAI
HAGLLARGEQDSPVLKQHGIKPIDLLVVNLYPFEQVINHPDCDFNKAIENIDIGGPAMVRAAAKNHAHTYVIVDPNDYPK
LIHYLQDQKAPSHWNFALAKKAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFNKITDLRYGENPHQQAIFYADKN
SHPGSLGTAALLQGKQLSYNNILDADAALDCVKSFSNEKSVCVIVKHTNPCGIALSDTSLDAYLKAFQSDPISAYGGIIA
FNGTLDSDTAKAILEKQFVEVIIAPDANEEAKKILAAKENIRVLLTGFWQQGTNFRLNMKKVDGGLLVQEHDSLSLESCE
LQTVTQAKPTDEQLQNLMFAWLAAKHVKSNAIVYANDLATIGIGGGQTSRVMSARIGLWQAEQMGFDPQGAVMASDAFIP
FPDTIEIAAKAGISAIIQPGGSIRDEKIISCADQHNIAMIFTGVRHFKH
>Mature_528_residues
AKKQIYSSFNPRRALLSVSDKRGIRELGQALHDHGVELIATGNTAAILREHKLPVTDVSECTGFPEIMDGRVKTLHPAIH
AGLLARGEQDSPVLKQHGIKPIDLLVVNLYPFEQVINHPDCDFNKAIENIDIGGPAMVRAAAKNHAHTYVIVDPNDYPKL
IHYLQDQKAPSHWNFALAKKAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFNKITDLRYGENPHQQAIFYADKNS
HPGSLGTAALLQGKQLSYNNILDADAALDCVKSFSNEKSVCVIVKHTNPCGIALSDTSLDAYLKAFQSDPISAYGGIIAF
NGTLDSDTAKAILEKQFVEVIIAPDANEEAKKILAAKENIRVLLTGFWQQGTNFRLNMKKVDGGLLVQEHDSLSLESCEL
QTVTQAKPTDEQLQNLMFAWLAAKHVKSNAIVYANDLATIGIGGGQTSRVMSARIGLWQAEQMGFDPQGAVMASDAFIPF
PDTIEIAAKAGISAIIQPGGSIRDEKIISCADQHNIAMIFTGVRHFKH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=475, Percent_Identity=34.3157894736842, Blast_Score=216, Evalue=4e-56,
Organism=Escherichia coli, GI1790439, Length=525, Percent_Identity=53.7142857142857, Blast_Score=565, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI71985564, Length=606, Percent_Identity=30.5280528052805, Blast_Score=249, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI71985556, Length=321, Percent_Identity=23.0529595015576, Blast_Score=78, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71985574, Length=348, Percent_Identity=23.8505747126437, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6323056, Length=474, Percent_Identity=33.7552742616034, Blast_Score=220, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6323768, Length=486, Percent_Identity=33.3333333333333, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24649832, Length=484, Percent_Identity=34.297520661157, Blast_Score=217, Evalue=2e-56,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57745; Mature: 57614

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKQIYSSFNPRRALLSVSDKRGIRELGQALHDHGVELIATGNTAAILREHKLPVTDVS
CCCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCEEEEECCCCEEEHHHCCCCCCCHH
ECTGFPEIMDGRVKTLHPAIHAGLLARGEQDSPVLKQHGIKPIDLLVVNLYPFEQVINHP
HHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEEEECCHHHHCCCC
DCDFNKAIENIDIGGPAMVRAAAKNHAHTYVIVDPNDYPKLIHYLQDQKAPSHWNFALAK
CCCHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHH
KAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFNKITDLRYGENPHQQAIFYADKN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEECCCCCCCCCCCEEEEECCC
SHPGSLGTAALLQGKQLSYNNILDADAALDCVKSFSNEKSVCVIVKHTNPCGIALSDTSL
CCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCH
DAYLKAFQSDPISAYGGIIAFNGTLDSDTAKAILEKQFVEVIIAPDANEEAKKILAAKEN
HHHHHHHCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCC
IRVLLTGFWQQGTNFRLNMKKVDGGLLVQEHDSLSLESCELQTVTQAKPTDEQLQNLMFA
CEEEEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHH
WLAAKHVKSNAIVYANDLATIGIGGGQTSRVMSARIGLWQAEQMGFDPQGAVMASDAFIP
HHHHHHHCCCEEEEECCEEEEECCCCCHHHHHHHHHCCEEHHHCCCCCCCCEEECCCCCC
FPDTIEIAAKAGISAIIQPGGSIRDEKIISCADQHNIAMIFTGVRHFKH
CCCHHHHHHHCCCCEEECCCCCCCCCHHHHHCCCCCEEEEEECHHHHCC
>Mature Secondary Structure 
AKKQIYSSFNPRRALLSVSDKRGIRELGQALHDHGVELIATGNTAAILREHKLPVTDVS
CCHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHCCEEEEECCCCEEEHHHCCCCCCCHH
ECTGFPEIMDGRVKTLHPAIHAGLLARGEQDSPVLKQHGIKPIDLLVVNLYPFEQVINHP
HHCCCHHHHCCCHHHHHHHHHHHHHHCCCCCCCHHHHCCCCEEEEEEEEECCHHHHCCCC
DCDFNKAIENIDIGGPAMVRAAAKNHAHTYVIVDPNDYPKLIHYLQDQKAPSHWNFALAK
CCCHHHHHHCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHH
KAFAHTAAYDAAIANYLTTLDNDYVPTGFPDILTCQFNKITDLRYGENPHQQAIFYADKN
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEECCCCCCCCCCCEEEEECCC
SHPGSLGTAALLQGKQLSYNNILDADAALDCVKSFSNEKSVCVIVKHTNPCGIALSDTSL
CCCCCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEECCCH
DAYLKAFQSDPISAYGGIIAFNGTLDSDTAKAILEKQFVEVIIAPDANEEAKKILAAKEN
HHHHHHHCCCCCHHHCCEEEECCCCCCHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHCC
IRVLLTGFWQQGTNFRLNMKKVDGGLLVQEHDSLSLESCELQTVTQAKPTDEQLQNLMFA
CEEEEEEECCCCCCEEEEEEECCCCEEEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHH
WLAAKHVKSNAIVYANDLATIGIGGGQTSRVMSARIGLWQAEQMGFDPQGAVMASDAFIP
HHHHHHHCCCEEEEECCEEEEECCCCCHHHHHHHHHCCEEHHHCCCCCCCCEEECCCCCC
FPDTIEIAAKAGISAIIQPGGSIRDEKIISCADQHNIAMIFTGVRHFKH
CCCHHHHHHHCCCCEEECCCCCCCCCHHHHHCCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA