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The map label for this gene is ecfE [C]

Identifier: 148360880

GI number: 148360880

Start: 597143

End: 598495

Strand: Direct

Name: ecfE [C]

Synonym: LPC_2840

Alternate gene names: 148360880

Gene position: 597143-598495 (Clockwise)

Preceding gene: 148360881

Following gene: 148360879

Centisome position: 16.7

GC content: 39.76

Gene sequence:

>1353_bases
ATGCTTTCAACGTTGCTGTACTTTTTATTAGCATTGGTATTGTTAGTCACCATACATGAATACGGCCACTTTCAAGTAGC
GCGATGGTGTGGTGTGAAAGTGTTAAGGTTTTCATTTGGCTTTGGAAAGATTCTTGCGCGTTTTTATGATAAGAAAGGAA
CAGAGTATGCCTGGTCTTTGTTTCCTTTGGGTGGCTATGTCAAAATGCTTGATGAAACAGAAGGAGAAGTTTCTGAAAAA
GAAAAGCCTTTCGCATTCAATAATCAATCTGTTCTTGTAAGGATAGCTATTGTAGTAGCAGGGCCTTTATTCAATTTTAT
TTTTGCGTTTGTAGCATTGTGGTTGGTTTTGGTTATTGGTATGCACTCTTTGGCTCCGATGATTGAGTCAGTAAAACCAA
ATAGTATTGCTGCACGAGCTGGTCTACTCCCCAAGCAGGAAATCCTTGCTCTTAATGGAGTTAAAATTAATAGTTGGCGA
GATTTTCAATATGAAATTATGCCTCTTATCGGCTCTCAGGAAACAGTGAGCTTGACTGTAAAGTCAATGATAAATGGTGA
AGAAAGCACGTTGTATTTGCCTTTGGATAAGTGGCAGTTGGATGATAAAAAGCCTGATCCTATTAAAAGCCTGGGCATCG
TTCCTTTTATACCCACTATTCCACCCATAGTCGGTGAAGTGGTTCCCGATTCTCCCGCTGAAAAAGCAGGCTTAAAAATA
GGGGATGAAATTATTAGTGTCAATGGTCAGCATTTCAATGATTGGTTGTATTTAGTCAGCTATGTAAGGGAAAGGCCCAA
TTCTCAAATCAATTTGGATATTAAACGCCAAGGTAAATTGCTGAAGATAACGGTACACACTGGAAGTCAGGATAATAATG
GAAAATTAGAGGGGTTAATAGGAGTTAGATCTCAGAAAGTAGATTGGCCTGCTCATTGGCTACGGCTGGAGCAACAGCCG
CCAATTTCAGCACTTGGTACGGCTTTTAAGCAAACAATACAACTCACAGGCACAACTTTTATCTTAATGGGAAGATTAGT
GACTGGAAAATTGGGATTAAACAGTATTAGTGGTCCCGTAGGTATAGCCCAGGGGGCCGGTGATTCCGGACGCGGAGGAT
TGGTATCCTATTTATTTTTTCTGGCATTAGTAAGCATTAGCTTGGGAGCGCTTAATTTATTACCAATCCCTATGCTGGAT
GGCGGTCATCTTTTGTATTATGTCTTGGAAATCATAAGAAGAAAGCCACTATCGGACGGAGTTAAATCTGTCGGGATCTA
TTTTGGATTATTACTGTTGGTTGCCTTAATGTTTGTTGCTCTTAGTAATGATATATCAAGATTAACCAGTTAG

Upstream 100 bases:

>100_bases
TGGATAGCCTAATTGCCGCGTTACCATGGTTTTACTTTGGATTAACCTACATACCACTGGGAATTTAATTTTCTGGTCGT
ATCAATCTTTGGATAACGAT

Downstream 100 bases:

>100_bases
GATTTGGCGAGAGTAATTCTAATTTCTGTATGATTAATGAATGAAATGATGTTTTAGCGACAAAGAAACTTGACAGTAGT
TTCTAGTTCCTATAAAAGGG

Product: membrane associated zinc metalloprotease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVLRFSFGFGKILARFYDKKGTEYAWSLFPLGGYVKMLDETEGEVSEK
EKPFAFNNQSVLVRIAIVVAGPLFNFIFAFVALWLVLVIGMHSLAPMIESVKPNSIAARAGLLPKQEILALNGVKINSWR
DFQYEIMPLIGSQETVSLTVKSMINGEESTLYLPLDKWQLDDKKPDPIKSLGIVPFIPTIPPIVGEVVPDSPAEKAGLKI
GDEIISVNGQHFNDWLYLVSYVRERPNSQINLDIKRQGKLLKITVHTGSQDNNGKLEGLIGVRSQKVDWPAHWLRLEQQP
PISALGTAFKQTIQLTGTTFILMGRLVTGKLGLNSISGPVGIAQGAGDSGRGGLVSYLFFLALVSISLGALNLLPIPMLD
GGHLLYYVLEIIRRKPLSDGVKSVGIYFGLLLLVALMFVALSNDISRLTS

Sequences:

>Translated_450_residues
MLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVLRFSFGFGKILARFYDKKGTEYAWSLFPLGGYVKMLDETEGEVSEK
EKPFAFNNQSVLVRIAIVVAGPLFNFIFAFVALWLVLVIGMHSLAPMIESVKPNSIAARAGLLPKQEILALNGVKINSWR
DFQYEIMPLIGSQETVSLTVKSMINGEESTLYLPLDKWQLDDKKPDPIKSLGIVPFIPTIPPIVGEVVPDSPAEKAGLKI
GDEIISVNGQHFNDWLYLVSYVRERPNSQINLDIKRQGKLLKITVHTGSQDNNGKLEGLIGVRSQKVDWPAHWLRLEQQP
PISALGTAFKQTIQLTGTTFILMGRLVTGKLGLNSISGPVGIAQGAGDSGRGGLVSYLFFLALVSISLGALNLLPIPMLD
GGHLLYYVLEIIRRKPLSDGVKSVGIYFGLLLLVALMFVALSNDISRLTS
>Mature_450_residues
MLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVLRFSFGFGKILARFYDKKGTEYAWSLFPLGGYVKMLDETEGEVSEK
EKPFAFNNQSVLVRIAIVVAGPLFNFIFAFVALWLVLVIGMHSLAPMIESVKPNSIAARAGLLPKQEILALNGVKINSWR
DFQYEIMPLIGSQETVSLTVKSMINGEESTLYLPLDKWQLDDKKPDPIKSLGIVPFIPTIPPIVGEVVPDSPAEKAGLKI
GDEIISVNGQHFNDWLYLVSYVRERPNSQINLDIKRQGKLLKITVHTGSQDNNGKLEGLIGVRSQKVDWPAHWLRLEQQP
PISALGTAFKQTIQLTGTTFILMGRLVTGKLGLNSISGPVGIAQGAGDSGRGGLVSYLFFLALVSISLGALNLLPIPMLD
GGHLLYYVLEIIRRKPLSDGVKSVGIYFGLLLLVALMFVALSNDISRLTS

Specific function: Unknown

COG id: COG0750

COG function: function code M; Predicted membrane-associated Zn-dependent proteases 1

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PDZ (DHR) domain [H]

Homologues:

Organism=Escherichia coli, GI1786373, Length=448, Percent_Identity=43.75, Blast_Score=352, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001478
- InterPro:   IPR004387
- InterPro:   IPR008915 [H]

Pfam domain/function: PF00595 PDZ; PF02163 Peptidase_M50 [H]

EC number: NA

Molecular weight: Translated: 49577; Mature: 49577

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50106 PDZ ; PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVLRFSFGFGKILARFYDKKGTEYAWSL
CHHHHHHHHHHHHHHHHHHHCCCEEHHHHHCHHHEEHHCCHHHHHHHHHHCCCCCEEEEE
FPLGGYVKMLDETEGEVSEKEKPFAFNNQSVLVRIAIVVAGPLFNFIFAFVALWLVLVIG
ECCCCHHHHHHCCCCCCCCCCCCCEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
MHSLAPMIESVKPNSIAARAGLLPKQEILALNGVKINSWRDFQYEIMPLIGSQETVSLTV
HHHHHHHHHCCCCCCHHHHHCCCCHHHHHEECCEEECCCCCCEEEEEEECCCCCCCEEEH
KSMINGEESTLYLPLDKWQLDDKKPDPIKSLGIVPFIPTIPPIVGEVVPDSPAEKAGLKI
HHHHCCCCCEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHCCCCCCCHHCCCEE
GDEIISVNGQHFNDWLYLVSYVRERPNSQINLDIKRQGKLLKITVHTGSQDNNGKLEGLI
CCHHEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCCEEEEE
GVRSQKVDWPAHWLRLEQQPPISALGTAFKQTIQLTGTTFILMGRLVTGKLGLNSISGPV
ECCCCCCCCCHHHHEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC
GIAQGAGDSGRGGLVSYLFFLALVSISLGALNLLPIPMLDGGHLLYYVLEIIRRKPLSDG
EECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
VKSVGIYFGLLLLVALMFVALSNDISRLTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSTLLYFLLALVLLVTIHEYGHFQVARWCGVKVLRFSFGFGKILARFYDKKGTEYAWSL
CHHHHHHHHHHHHHHHHHHHCCCEEHHHHHCHHHEEHHCCHHHHHHHHHHCCCCCEEEEE
FPLGGYVKMLDETEGEVSEKEKPFAFNNQSVLVRIAIVVAGPLFNFIFAFVALWLVLVIG
ECCCCHHHHHHCCCCCCCCCCCCCEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
MHSLAPMIESVKPNSIAARAGLLPKQEILALNGVKINSWRDFQYEIMPLIGSQETVSLTV
HHHHHHHHHCCCCCCHHHHHCCCCHHHHHEECCEEECCCCCCEEEEEEECCCCCCCEEEH
KSMINGEESTLYLPLDKWQLDDKKPDPIKSLGIVPFIPTIPPIVGEVVPDSPAEKAGLKI
HHHHCCCCCEEEEECCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHCCCCCCCHHCCCEE
GDEIISVNGQHFNDWLYLVSYVRERPNSQINLDIKRQGKLLKITVHTGSQDNNGKLEGLI
CCHHEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEEECCCCCCCCCEEEEE
GVRSQKVDWPAHWLRLEQQPPISALGTAFKQTIQLTGTTFILMGRLVTGKLGLNSISGPV
ECCCCCCCCCHHHHEECCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCC
GIAQGAGDSGRGGLVSYLFFLALVSISLGALNLLPIPMLDGGHLLYYVLEIIRRKPLSDG
EECCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHH
VKSVGIYFGLLLLVALMFVALSNDISRLTS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]