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The map label for this gene is ttcA [H]

Identifier: 148360650

GI number: 148360650

Start: 871763

End: 872614

Strand: Reverse

Name: ttcA [H]

Synonym: LPC_2596

Alternate gene names: 148360650

Gene position: 872614-871763 (Counterclockwise)

Preceding gene: 148360649

Following gene: 148360651

Centisome position: 24.4

GC content: 38.38

Gene sequence:

>852_bases
ATGTCTTCCAATCCTTCTTCAGTTGAAAAAAAATTACTCCATTATACAGGTAAAGCCATAGCTGATTTTAATATGATCCG
GCATGGTGACAGAGTCATGGTATGTCTGTCTGGCGGAAAAGATTCATTCACACTATTAACAATTCTTAACCAGCTCAGGA
TCAAATCAGGAAATAAATTTGAAATTTTTGCGTTTACTCTGGATCAAGCCCAGCCAGGGTGGAATGATGCCTGTTTGCGT
CAATGGTTGGCTGAAAAATCCATTCCTCATGAAATACTAACCCGAGACACATACAGTATAGTGAAGGAAAAAATTCCTGA
AGGCAAAACCTACTGTTCTTTGTGTTCTCGCTTGAGACGTGGAATCATCTACAGATATGCTGAAGAAAACGGATTTAATA
AAATAGCATTAGGTCACCATCGTGATGATCTTATTCGTACATTGATGATGTCAATTCTATACAATGGCGATATACGTTCC
ATGCCACCCAAACTACTGAGTGATAATAAAAAGCACATAGTCATCCGGCCACTGTGCTATGTACAGGAAAAAGATATTAT
TACGTTTGCATCAGAACAAGCCTTTCCAATTATCCCATGCAATCTTTGTGGCTCTCAAGAAAATCTAATGCGCAAAAAAG
TTGCCTCACTCATTGATCAGCTTGCGATTGAAAACCCCAAAGTCCCCAGTAACATGCTTCATGCGCTGCAAAGTTTGAAG
CCAAGTCAATTGATGGATCAAAATTTTTGGAATTTTAAAAATCTGGAAGATGGGCTTGAAACCGCGCAGTCAGTTCAATG
TGAAGAAGTATTTAATGCACAGGAATTTGAGTTTGAGGATGAAAAAATTTGA

Upstream 100 bases:

>100_bases
ATGATCTCGAACAGATTAACTCATGGTTAGCAACTACACGTGTCAATGGATTTTCACCCGTAGATAACAAAACCTCCAAA
TAACAAATTTAGGTTTTCTT

Downstream 100 bases:

>100_bases
CTTATGAAAATTATTATCTAATATTTCTTTTCATTTTTGCAATATAATGAAATAATTGACACGTAAAATAAGCTTATTAT
TTTGACAAATGGAATTAAAT

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MSSNPSSVEKKLLHYTGKAIADFNMIRHGDRVMVCLSGGKDSFTLLTILNQLRIKSGNKFEIFAFTLDQAQPGWNDACLR
QWLAEKSIPHEILTRDTYSIVKEKIPEGKTYCSLCSRLRRGIIYRYAEENGFNKIALGHHRDDLIRTLMMSILYNGDIRS
MPPKLLSDNKKHIVIRPLCYVQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLIDQLAIENPKVPSNMLHALQSLK
PSQLMDQNFWNFKNLEDGLETAQSVQCEEVFNAQEFEFEDEKI

Sequences:

>Translated_283_residues
MSSNPSSVEKKLLHYTGKAIADFNMIRHGDRVMVCLSGGKDSFTLLTILNQLRIKSGNKFEIFAFTLDQAQPGWNDACLR
QWLAEKSIPHEILTRDTYSIVKEKIPEGKTYCSLCSRLRRGIIYRYAEENGFNKIALGHHRDDLIRTLMMSILYNGDIRS
MPPKLLSDNKKHIVIRPLCYVQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLIDQLAIENPKVPSNMLHALQSLK
PSQLMDQNFWNFKNLEDGLETAQSVQCEEVFNAQEFEFEDEKI
>Mature_282_residues
SSNPSSVEKKLLHYTGKAIADFNMIRHGDRVMVCLSGGKDSFTLLTILNQLRIKSGNKFEIFAFTLDQAQPGWNDACLRQ
WLAEKSIPHEILTRDTYSIVKEKIPEGKTYCSLCSRLRRGIIYRYAEENGFNKIALGHHRDDLIRTLMMSILYNGDIRSM
PPKLLSDNKKHIVIRPLCYVQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLIDQLAIENPKVPSNMLHALQSLKP
SQLMDQNFWNFKNLEDGLETAQSVQCEEVFNAQEFEFEDEKI

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32) [H]

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family [H]

Homologues:

Organism=Escherichia coli, GI1787606, Length=280, Percent_Identity=50.3571428571429, Blast_Score=288, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI193211090, Length=234, Percent_Identity=32.0512820512821, Blast_Score=118, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17540090, Length=183, Percent_Identity=28.4153005464481, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24651972, Length=239, Percent_Identity=25.9414225941423, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 32434; Mature: 32303

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNPSSVEKKLLHYTGKAIADFNMIRHGDRVMVCLSGGKDSFTLLTILNQLRIKSGNKF
CCCCHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEE
EIFAFTLDQAQPGWNDACLRQWLAEKSIPHEILTRDTYSIVKEKIPEGKTYCSLCSRLRR
EEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
GIIYRYAEENGFNKIALGHHRDDLIRTLMMSILYNGDIRSMPPKLLSDNKKHIVIRPLCY
HHEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEECCCHH
VQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLIDQLAIENPKVPSNMLHALQSLK
CCCCCEEEEECCCCCCEEEHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
PSQLMDQNFWNFKNLEDGLETAQSVQCEEVFNAQEFEFEDEKI
HHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHCCHHCCCCCCCC
>Mature Secondary Structure 
SSNPSSVEKKLLHYTGKAIADFNMIRHGDRVMVCLSGGKDSFTLLTILNQLRIKSGNKF
CCCHHHHHHHHHHHHCHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCEE
EIFAFTLDQAQPGWNDACLRQWLAEKSIPHEILTRDTYSIVKEKIPEGKTYCSLCSRLRR
EEEEEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
GIIYRYAEENGFNKIALGHHRDDLIRTLMMSILYNGDIRSMPPKLLSDNKKHIVIRPLCY
HHEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEECCCHH
VQEKDIITFASEQAFPIIPCNLCGSQENLMRKKVASLIDQLAIENPKVPSNMLHALQSLK
CCCCCEEEEECCCCCCEEEHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCC
PSQLMDQNFWNFKNLEDGLETAQSVQCEEVFNAQEFEFEDEKI
HHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA