Click here to switch to the map view.

The map label for this gene is purB

Identifier: 148360552

GI number: 148360552

Start: 982037

End: 983407

Strand: Reverse

Name: purB

Synonym: LPC_2494

Alternate gene names: 148360552

Gene position: 983407-982037 (Counterclockwise)

Preceding gene: 148360550

Following gene: 148360553

Centisome position: 27.5

GC content: 38.58

Gene sequence:

>1371_bases
ATGACACTTACTGCATTAAACGCGATTTCACCCATAGATGGTCGTTATGTCAATAAAACCAGAGCATTAAGTCCCTATTT
TAGTGAGTTTGCATTAACCTATTATCGTTTAATGGTTGAAATCAAGTGGTTTGAATCATTAGCTGCTAACGACACTATTC
CAGAAGTACCTGCTTTGGATAACAAGGCTCGTAAATTTTTATCTGATCTTATTTCCAATTTTAATGAATCAGAAGCTGAA
AAAATAAAAGAATTTGAAAAACAAACCAATCATGACGTTAAAGCCGTAGAGTATTATCTTAAGGATAAATTTCAGGAAAA
TGAACAACTCAAATCTTGCGTTGCTTTCATACACTTTGCCTGCACTTCAGAGGACATCAATAACCTGGCTTATGCTTTAA
TGATCAAACAAGCGATAGCCCAAGTTATACAACCTACTATAGCTGAGATCATGGGAAGCATTACGCTTCTTGGAAAACAA
CATGCTGACGTTGCGATGCTCTCAAGAACGCATGGTCAACCAGCAACACCAACCACCATGGGTAAAGAATTGGTCAATTT
TGTAGCTCGCCTTAAACGACCCCAGCAACAATTGGCAGAAGTATTAATCCCTGCCAAATTTAATGGAGCTGTTGGCAATT
ACAATGCTCACGTAGCAGCCTATCCCGAAGTGGACTGGCGTAAGCATTGCGCTAATTTTGTTACATCCCTAGGTTTATCC
TTTAACGCCTATACCACTCAAATTGAACCACATGATGGCATAGCGGAAGTCTCCCAGATTATGGTGAGAATTAATAATAT
TCTTCTGGATTATACCCAAGACATATGGAGCTATATTTCTCTTGGCTACTTTAAGCAAAAAACAATTGCCGAAGAAGTAG
GCTCATCGACCATGCCGCACAAGGTAAATCCTATAGACTTTGAAAATGCCGAAGGGAATTTAGGTCTGTCTAATGCTTTA
TTTATTCATTTTGCCAACAAATTAACTCAATCACGCATGCAAAGAGACCTGTCTGATTCGACTGTCTTACGTAACTTGGG
CGTAGCCTTCTCTTACAGTTTGATTGCCTATCACTCAGTAGCCAAAGGTAATGATAAATTACAAATCAATAAATCAGCTC
TACAAAAAGATTTATCCGAAAACTGGGAAGTTTTAGCAGAGGCCATACAAACTGTCATGCGCCGCTACAATGAACCCAAT
GCCTATGAACAATTAAAAGAGCTTACACGAGGACAAATGATAGACGCTGAAAACCTTAAAAAATTTATTAAAACCCTGTC
TATTCCCGAAGAAGCAAAAGCAGAGTTAATGAAATTAACTCCAGAGACTTACACCGGTCTTGCAACCCAATTGGTCAAGG
CTTTTTCATGA

Upstream 100 bases:

>100_bases
CAAATTTACGCTAATTTTCTAGAATATTTATCGCTTTAATTCACATCTTGTCCTGCATCTAATATTATATACGCTTTAAT
ATTGGATTAAGCTACCTATC

Downstream 100 bases:

>100_bases
GTGATCAGCTATCACAACAAGGCCAATCGTTCGAATTCGATGTTCTCGTCATTGGAACAGGATTAGCCGGGTTACATTAT
TGTTTGCAGCTTTTAGCTAT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MTLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWFESLAANDTIPEVPALDNKARKFLSDLISNFNESEAE
KIKEFEKQTNHDVKAVEYYLKDKFQENEQLKSCVAFIHFACTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQ
HADVAMLSRTHGQPATPTTMGKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHVAAYPEVDWRKHCANFVTSLGLS
FNAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIWSYISLGYFKQKTIAEEVGSSTMPHKVNPIDFENAEGNLGLSNAL
FIHFANKLTQSRMQRDLSDSTVLRNLGVAFSYSLIAYHSVAKGNDKLQINKSALQKDLSENWEVLAEAIQTVMRRYNEPN
AYEQLKELTRGQMIDAENLKKFIKTLSIPEEAKAELMKLTPETYTGLATQLVKAFS

Sequences:

>Translated_456_residues
MTLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWFESLAANDTIPEVPALDNKARKFLSDLISNFNESEAE
KIKEFEKQTNHDVKAVEYYLKDKFQENEQLKSCVAFIHFACTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQ
HADVAMLSRTHGQPATPTTMGKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHVAAYPEVDWRKHCANFVTSLGLS
FNAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIWSYISLGYFKQKTIAEEVGSSTMPHKVNPIDFENAEGNLGLSNAL
FIHFANKLTQSRMQRDLSDSTVLRNLGVAFSYSLIAYHSVAKGNDKLQINKSALQKDLSENWEVLAEAIQTVMRRYNEPN
AYEQLKELTRGQMIDAENLKKFIKTLSIPEEAKAELMKLTPETYTGLATQLVKAFS
>Mature_455_residues
TLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWFESLAANDTIPEVPALDNKARKFLSDLISNFNESEAEK
IKEFEKQTNHDVKAVEYYLKDKFQENEQLKSCVAFIHFACTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQH
ADVAMLSRTHGQPATPTTMGKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHVAAYPEVDWRKHCANFVTSLGLSF
NAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIWSYISLGYFKQKTIAEEVGSSTMPHKVNPIDFENAEGNLGLSNALF
IHFANKLTQSRMQRDLSDSTVLRNLGVAFSYSLIAYHSVAKGNDKLQINKSALQKDLSENWEVLAEAIQTVMRRYNEPNA
YEQLKELTRGQMIDAENLKKFIKTLSIPEEAKAELMKLTPETYTGLATQLVKAFS

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Homologues:

Organism=Escherichia coli, GI1787376, Length=452, Percent_Identity=56.858407079646, Blast_Score=541, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR8_LEGPC (A5IGB3)

Other databases:

- EMBL:   CP000675
- RefSeq:   YP_001251759.1
- ProteinModelPortal:   A5IGB3
- SMR:   A5IGB3
- STRING:   A5IGB3
- GeneID:   5180323
- GenomeReviews:   CP000675_GR
- KEGG:   lpc:LPC_2494
- eggNOG:   COG0015
- HOGENOM:   HBG290028
- OMA:   KVSEFIH
- ProtClustDB:   PRK09285
- BioCyc:   LPNE400673:LPC_2494-MONOMER
- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00928

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.2

Molecular weight: Translated: 51403; Mature: 51272

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: ACT_SITE 91-91 ACT_SITE 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWFESLAANDTIPEVPALD
CCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NKARKFLSDLISNFNESEAEKIKEFEKQTNHDVKAVEYYLKDKFQENEQLKSCVAFIHFA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
CTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQHADVAMLSRTHGQPATPTTM
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHH
GKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHVAAYPEVDWRKHCANFVTSLGLS
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCC
FNAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIWSYISLGYFKQKTIAEEVGSSTMPH
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KVNPIDFENAEGNLGLSNALFIHFANKLTQSRMQRDLSDSTVLRNLGVAFSYSLIAYHSV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AKGNDKLQINKSALQKDLSENWEVLAEAIQTVMRRYNEPNAYEQLKELTRGQMIDAENLK
HCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHH
KFIKTLSIPEEAKAELMKLTPETYTGLATQLVKAFS
HHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLTALNAISPIDGRYVNKTRALSPYFSEFALTYYRLMVEIKWFESLAANDTIPEVPALD
CCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
NKARKFLSDLISNFNESEAEKIKEFEKQTNHDVKAVEYYLKDKFQENEQLKSCVAFIHFA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
CTSEDINNLAYALMIKQAIAQVIQPTIAEIMGSITLLGKQHADVAMLSRTHGQPATPTTM
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCHHH
GKELVNFVARLKRPQQQLAEVLIPAKFNGAVGNYNAHVAAYPEVDWRKHCANFVTSLGLS
HHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHCCC
FNAYTTQIEPHDGIAEVSQIMVRINNILLDYTQDIWSYISLGYFKQKTIAEEVGSSTMPH
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
KVNPIDFENAEGNLGLSNALFIHFANKLTQSRMQRDLSDSTVLRNLGVAFSYSLIAYHSV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
AKGNDKLQINKSALQKDLSENWEVLAEAIQTVMRRYNEPNAYEQLKELTRGQMIDAENLK
HCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCHHHHH
KFIKTLSIPEEAKAELMKLTPETYTGLATQLVKAFS
HHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA