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The map label for this gene is rluE [H]

Identifier: 148360539

GI number: 148360539

Start: 998945

End: 999604

Strand: Reverse

Name: rluE [H]

Synonym: LPC_2481

Alternate gene names: 148360539

Gene position: 999604-998945 (Counterclockwise)

Preceding gene: 148360532

Following gene: 148360549

Centisome position: 27.95

GC content: 38.64

Gene sequence:

>660_bases
TTGCTGTCAATAACCAAAAAATTTATCATTAATTTCCATAATCAACGCAATCATAAAATGCCACAATTAATACTTTTTAA
TAAACCTTATGGAGTCCTGACTCAATTTACTGGCGAATTACCCGAACAAACTCTCTCGGCATTTATTAAATTTCCGGGTT
TTTATGCAGCTGGACGTCTGGATAAACAAAGCGAAGGCTTGCTTGTCTTAACTGACGATGGAAAACTACAACACCGGCTA
ACCCATCCTAAATTTGAAAAAAAGAAACATTACTGGGTTCAAGTAGAGGGGGCCCCTACAGACAAAGATTTACAACCTTT
AAGAAAAGGCCTGGTAATCAAAGACATTACTTTTTTACCCGCCGAAGCCAAGTTGATTTCAGAGCCAAAAATATGGCCAC
GAATTCCGCCTATAAGAGAACGGAAACACATACCAACTACCTGGCTGGAAATTATTTTAAGAGAAGGGAAAAATCATCAA
ATAAGAAAAATGACCGCGGCTATTGGATTTCCTACTTTACGTTTAATTAGACACCAGATTGGTGATTGGCGGTTAGATCA
ACTCCAGCCCGGGGAATATAAGTTAATCAACCTTACTAACGATAAACTGAACGAGTTAACAAGGAAGAGTAACCCAAGAC
TCGGATCCAGGGCCAAATAA

Upstream 100 bases:

>100_bases
ATTAATTACTGCACCTCATATCTCATTGAGTGAATGACAAAATATGAACAATTATTTACGCTGTTATTGTGCTTAAAGGC
GTGATCTAGCCCTAAAGTGG

Downstream 100 bases:

>100_bases
TCATACCCAAAATGGCACTTGCTACAGGCATTATCAGGTTATAATTAAAACCTAACAGAGCATCAATAAATATAATAATT
GATTGATATAGAAAACAAAA

Product: pseudouridine synthase

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MLSITKKFIINFHNQRNHKMPQLILFNKPYGVLTQFTGELPEQTLSAFIKFPGFYAAGRLDKQSEGLLVLTDDGKLQHRL
THPKFEKKKHYWVQVEGAPTDKDLQPLRKGLVIKDITFLPAEAKLISEPKIWPRIPPIRERKHIPTTWLEIILREGKNHQ
IRKMTAAIGFPTLRLIRHQIGDWRLDQLQPGEYKLINLTNDKLNELTRKSNPRLGSRAK

Sequences:

>Translated_219_residues
MLSITKKFIINFHNQRNHKMPQLILFNKPYGVLTQFTGELPEQTLSAFIKFPGFYAAGRLDKQSEGLLVLTDDGKLQHRL
THPKFEKKKHYWVQVEGAPTDKDLQPLRKGLVIKDITFLPAEAKLISEPKIWPRIPPIRERKHIPTTWLEIILREGKNHQ
IRKMTAAIGFPTLRLIRHQIGDWRLDQLQPGEYKLINLTNDKLNELTRKSNPRLGSRAK
>Mature_219_residues
MLSITKKFIINFHNQRNHKMPQLILFNKPYGVLTQFTGELPEQTLSAFIKFPGFYAAGRLDKQSEGLLVLTDDGKLQHRL
THPKFEKKKHYWVQVEGAPTDKDLQPLRKGLVIKDITFLPAEAKLISEPKIWPRIPPIRERKHIPTTWLEIILREGKNHQ
IRKMTAAIGFPTLRLIRHQIGDWRLDQLQPGEYKLINLTNDKLNELTRKSNPRLGSRAK

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=176, Percent_Identity=57.9545454545455, Blast_Score=206, Evalue=1e-54,
Organism=Escherichia coli, GI1788510, Length=178, Percent_Identity=31.4606741573034, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1787524, Length=170, Percent_Identity=31.1764705882353, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 25506; Mature: 25506

Theoretical pI: Translated: 10.86; Mature: 10.86

Prosite motif: PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSITKKFIINFHNQRNHKMPQLILFNKPYGVLTQFTGELPEQTLSAFIKFPGFYAAGRL
CCCCCEEHHEECCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHCCCCEECCCC
DKQSEGLLVLTDDGKLQHRLTHPKFEKKKHYWVQVEGAPTDKDLQPLRKGLVIKDITFLP
CCCCCCEEEEECCCCEEHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCEEEEEEECC
AEAKLISEPKIWPRIPPIRERKHIPTTWLEIILREGKNHQIRKMTAAIGFPTLRLIRHQI
CCHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHH
GDWRLDQLQPGEYKLINLTNDKLNELTRKSNPRLGSRAK
CCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLSITKKFIINFHNQRNHKMPQLILFNKPYGVLTQFTGELPEQTLSAFIKFPGFYAAGRL
CCCCCEEHHEECCCCCCCCCCEEEEEECCCHHHHHHHCCCHHHHHHHHHHCCCCEECCCC
DKQSEGLLVLTDDGKLQHRLTHPKFEKKKHYWVQVEGAPTDKDLQPLRKGLVIKDITFLP
CCCCCCEEEEECCCCEEHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHCCCEEEEEEECC
AEAKLISEPKIWPRIPPIRERKHIPTTWLEIILREGKNHQIRKMTAAIGFPTLRLIRHQI
CCHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHH
GDWRLDQLQPGEYKLINLTNDKLNELTRKSNPRLGSRAK
CCCCCCCCCCCCEEEEEECCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]