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The map label for this gene is glyS [H]

Identifier: 148359386

GI number: 148359386

Start: 2140144

End: 2142210

Strand: Reverse

Name: glyS [H]

Synonym: LPC_1284

Alternate gene names: 148359386

Gene position: 2142210-2140144 (Counterclockwise)

Preceding gene: 148359387

Following gene: 148359385

Centisome position: 59.9

GC content: 40.64

Gene sequence:

>2067_bases
ATGGTCAATGATTTTATTTTTGAATTAGGATGTGAAGAGCTGCCTTCTGGTGCTGTTTGGCCTTTGGCAAATGAGTTTTC
TACTCGATTGTTAGCCGCATTAGATAAGGCGCAATTAAGTTATGGTGAAGTCAAGAGATTCGCTACACCAAGACGTCTGG
CTATTGTGATTCATGACTTACAAACTGAACAGGAAAGCCAAAAAGTCATGCGACGCGGTCCTGCTGCAATAGCAGCATAC
GATAGTGCAGGTAACCCGACTCCTGCTTTGTTAGGATTTGCAAAATCATGTGGTGTTACGGTAGATGCGCTCACCAAGAC
TCAAACTGACAAAGGTGAATGGATTGTTTTTGAAACTCAAAGTGAGGGTACTAAAACAAAAGATCTCTTGCCTGGATTGG
TTAGTCAGTCTTTAGAGTCTTTACCTATCGCAAAACCCATGAGATGGGGAGAAGGAGACGACGAATTTGCCCGACCTGTC
CATTGGGCCGTGATGCTTTATGGAAGTGAGACACTTAATCATAAAATTTTAGGCGTTACAAGTGGATGTCATAGCAGGGG
ACATCGTTTTCATCATCCGCAGGAAATAAGAATAAATTCAGCTGCAAGCTATGAAGATCAGATGAAAGATGCTTTTGTAA
TTGCCGATTTTGCTACTAGGCGACAAGCCATTATCCATCAGGTACAAGAATTGGCGGCACCTTTAGGTGCTTCAGTGGTT
ATGCCTGAAGAATTGGTTGATGAAGTCACCTCAATAGTCGAATGGCCTCAAGCGCTCATTGCAAATTTTGAACAGGAATT
TTTAGAGGTTCCTGCTGAAGCCCTTATTGCTTCCATGCAATCTCATCAGAAGTGTTTTGCACTAAAAAATAAGCATGGTG
AGCTGCTACCTTATTTTATTACGGTATCAAATATCGCCAGCTCCAATCCAAAACAAGTGGTTTTAGGAAATGAGAAGGTA
ATGAGAGCAAGATTAAGTGATGCTGCCTTTTTCTTTAAACAAGATAAAAAACAGCCGCTAAGCGCTCATATACCTTCTAC
CGAGCAGGTTATTTTTCAAGTAAAGCTGGGAAGCGTGTATGATAAGGTACAACGAATAAAGGTAATGATGGATCATTTAA
GCCAGTCTCTCAATTTACCCTCTCACCTGGCTGAGCGAGCTACATTATTAAGCAAGTGTGATTTGATGACAGGTATGGTA
GGCGAATTTCCTGAATTGCAAGGGATTATGGGATATTATTATGCTCTTAATGATGGCGAAGAAGCTGTTGTTGCTACCGC
TTTAAATGAGCAATACATGCCTAGATTTTCTGGTGATGATTTACCATCGACTGATTTGGGTAAAGCACTTTCCCTGGCGG
ATCGTATGGATACTCTGGTGGGTATTTTTGCGATTGGCCAAAAGCCAACTGGAGTAAAAGATCCTTTTAAATTAAGACGC
CACGCTTTGGCTGTAGTCCGTTTACTCATTTCAATATCAGCACCGCTTAATTTATCAACTTTGATTAAAGAAGCCTTGGC
TAATTATGGTGATCGACTCCCTCAAGACAAGAATTTAGTGGCTGAATTAAAACCATTTATTCTTGAGAGGCTTCAATCTT
ATTATCAAAATCAAGGTATCAGCGCTGATTTGGTTCATGCTGTCCGTGCTCGTCAGGATGATTGGTTATATGATTTAGAT
AAGCGTCTATTCGCTTTAAAGTCATTTATCACTATGCCAGAGGCGACATCATTATCAGCTGCTTGTAAGCGTGTTGGCAA
TATCTTAGCTCAGGCAGCATATGCGGATAAAAAAGTAGATATTAAGGAAGAGCTTATAGAGGAAGGCGCTGAAAAAGCAT
TATTTGATCATCTTAATAAAATTACTAAAACAGTAGAAACTTTATACATTGCCGGAGATTACCAAGCATTACTCAAGCTT
CTGGCTAGTCTTAAAGAGCCAGTAGACGCTTTTTTTGATCATGTGATGGTTATGGTTGAAGAGGAGTCATTGAAACAGAA
CAGGCTTGCCCTCCTGCAGCGATTACAAGAGTTATTGCAGGGTGTAGCTGACATTTCAATGTTGTAG

Upstream 100 bases:

>100_bases
CAAAGGTATATATTACGTGTAAGACATCTATCAAGAGCAGTAGCTCAAGCTTACTATCAAGCGCGCGAGGCGTTAGGTTT
CCCTATGCAAAAGGTGTGTA

Downstream 100 bases:

>100_bases
CACTTATGAATCGAATTATATTGCTTGATAGAGATGGAGTAATTAACACTGACTCAATCAATTATATCAAGAATGTTGAT
GAGCTTATTATTATACCCGA

Product: glycyl tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MVNDFIFELGCEELPSGAVWPLANEFSTRLLAALDKAQLSYGEVKRFATPRRLAIVIHDLQTEQESQKVMRRGPAAIAAY
DSAGNPTPALLGFAKSCGVTVDALTKTQTDKGEWIVFETQSEGTKTKDLLPGLVSQSLESLPIAKPMRWGEGDDEFARPV
HWAVMLYGSETLNHKILGVTSGCHSRGHRFHHPQEIRINSAASYEDQMKDAFVIADFATRRQAIIHQVQELAAPLGASVV
MPEELVDEVTSIVEWPQALIANFEQEFLEVPAEALIASMQSHQKCFALKNKHGELLPYFITVSNIASSNPKQVVLGNEKV
MRARLSDAAFFFKQDKKQPLSAHIPSTEQVIFQVKLGSVYDKVQRIKVMMDHLSQSLNLPSHLAERATLLSKCDLMTGMV
GEFPELQGIMGYYYALNDGEEAVVATALNEQYMPRFSGDDLPSTDLGKALSLADRMDTLVGIFAIGQKPTGVKDPFKLRR
HALAVVRLLISISAPLNLSTLIKEALANYGDRLPQDKNLVAELKPFILERLQSYYQNQGISADLVHAVRARQDDWLYDLD
KRLFALKSFITMPEATSLSAACKRVGNILAQAAYADKKVDIKEELIEEGAEKALFDHLNKITKTVETLYIAGDYQALLKL
LASLKEPVDAFFDHVMVMVEEESLKQNRLALLQRLQELLQGVADISML

Sequences:

>Translated_688_residues
MVNDFIFELGCEELPSGAVWPLANEFSTRLLAALDKAQLSYGEVKRFATPRRLAIVIHDLQTEQESQKVMRRGPAAIAAY
DSAGNPTPALLGFAKSCGVTVDALTKTQTDKGEWIVFETQSEGTKTKDLLPGLVSQSLESLPIAKPMRWGEGDDEFARPV
HWAVMLYGSETLNHKILGVTSGCHSRGHRFHHPQEIRINSAASYEDQMKDAFVIADFATRRQAIIHQVQELAAPLGASVV
MPEELVDEVTSIVEWPQALIANFEQEFLEVPAEALIASMQSHQKCFALKNKHGELLPYFITVSNIASSNPKQVVLGNEKV
MRARLSDAAFFFKQDKKQPLSAHIPSTEQVIFQVKLGSVYDKVQRIKVMMDHLSQSLNLPSHLAERATLLSKCDLMTGMV
GEFPELQGIMGYYYALNDGEEAVVATALNEQYMPRFSGDDLPSTDLGKALSLADRMDTLVGIFAIGQKPTGVKDPFKLRR
HALAVVRLLISISAPLNLSTLIKEALANYGDRLPQDKNLVAELKPFILERLQSYYQNQGISADLVHAVRARQDDWLYDLD
KRLFALKSFITMPEATSLSAACKRVGNILAQAAYADKKVDIKEELIEEGAEKALFDHLNKITKTVETLYIAGDYQALLKL
LASLKEPVDAFFDHVMVMVEEESLKQNRLALLQRLQELLQGVADISML
>Mature_688_residues
MVNDFIFELGCEELPSGAVWPLANEFSTRLLAALDKAQLSYGEVKRFATPRRLAIVIHDLQTEQESQKVMRRGPAAIAAY
DSAGNPTPALLGFAKSCGVTVDALTKTQTDKGEWIVFETQSEGTKTKDLLPGLVSQSLESLPIAKPMRWGEGDDEFARPV
HWAVMLYGSETLNHKILGVTSGCHSRGHRFHHPQEIRINSAASYEDQMKDAFVIADFATRRQAIIHQVQELAAPLGASVV
MPEELVDEVTSIVEWPQALIANFEQEFLEVPAEALIASMQSHQKCFALKNKHGELLPYFITVSNIASSNPKQVVLGNEKV
MRARLSDAAFFFKQDKKQPLSAHIPSTEQVIFQVKLGSVYDKVQRIKVMMDHLSQSLNLPSHLAERATLLSKCDLMTGMV
GEFPELQGIMGYYYALNDGEEAVVATALNEQYMPRFSGDDLPSTDLGKALSLADRMDTLVGIFAIGQKPTGVKDPFKLRR
HALAVVRLLISISAPLNLSTLIKEALANYGDRLPQDKNLVAELKPFILERLQSYYQNQGISADLVHAVRARQDDWLYDLD
KRLFALKSFITMPEATSLSAACKRVGNILAQAAYADKKVDIKEELIEEGAEKALFDHLNKITKTVETLYIAGDYQALLKL
LASLKEPVDAFFDHVMVMVEEESLKQNRLALLQRLQELLQGVADISML

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=687, Percent_Identity=44.5414847161572, Blast_Score=577, Evalue=1e-166,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 76468; Mature: 76468

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNDFIFELGCEELPSGAVWPLANEFSTRLLAALDKAQLSYGEVKRFATPRRLAIVIHDL
CCCHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHH
QTEQESQKVMRRGPAAIAAYDSAGNPTPALLGFAKSCGVTVDALTKTQTDKGEWIVFETQ
HCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCEEEEEEC
SEGTKTKDLLPGLVSQSLESLPIAKPMRWGEGDDEFARPVHWAVMLYGSETLNHKILGVT
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCHHEEEEEEECCHHHCCEEEEEC
SGCHSRGHRFHHPQEIRINSAASYEDQMKDAFVIADFATRRQAIIHQVQELAAPLGASVV
CCHHHCCCCCCCCCCEEECCCCCHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCCCCC
MPEELVDEVTSIVEWPQALIANFEQEFLEVPAEALIASMQSHQKCFALKNKHGELLPYFI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
TVSNIASSNPKQVVLGNEKVMRARLSDAAFFFKQDKKQPLSAHIPSTEQVIFQVKLGSVY
EEHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEEEHHHHH
DKVQRIKVMMDHLSQSLNLPSHLAERATLLSKCDLMTGMVGEFPELQGIMGYYYALNDGE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCC
EAVVATALNEQYMPRFSGDDLPSTDLGKALSLADRMDTLVGIFAIGQKPTGVKDPFKLRR
CEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
HALAVVRLLISISAPLNLSTLIKEALANYGDRLPQDKNLVAELKPFILERLQSYYQNQGI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
SADLVHAVRARQDDWLYDLDKRLFALKSFITMPEATSLSAACKRVGNILAQAAYADKKVD
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
IKEELIEEGAEKALFDHLNKITKTVETLYIAGDYQALLKLLASLKEPVDAFFDHVMVMVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EESLKQNRLALLQRLQELLQGVADISML
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVNDFIFELGCEELPSGAVWPLANEFSTRLLAALDKAQLSYGEVKRFATPRRLAIVIHDL
CCCHHHHHHCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEHH
QTEQESQKVMRRGPAAIAAYDSAGNPTPALLGFAKSCGVTVDALTKTQTDKGEWIVFETQ
HCHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHCCCCHHHHHCCCCCCCCEEEEEEC
SEGTKTKDLLPGLVSQSLESLPIAKPMRWGEGDDEFARPVHWAVMLYGSETLNHKILGVT
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCHHEEEEEEECCHHHCCEEEEEC
SGCHSRGHRFHHPQEIRINSAASYEDQMKDAFVIADFATRRQAIIHQVQELAAPLGASVV
CCHHHCCCCCCCCCCEEECCCCCHHHHHHHHEEEHHHHHHHHHHHHHHHHHHHCCCCCCC
MPEELVDEVTSIVEWPQALIANFEQEFLEVPAEALIASMQSHQKCFALKNKHGELLPYFI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
TVSNIASSNPKQVVLGNEKVMRARLSDAAFFFKQDKKQPLSAHIPSTEQVIFQVKLGSVY
EEHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHEEEEEEHHHHH
DKVQRIKVMMDHLSQSLNLPSHLAERATLLSKCDLMTGMVGEFPELQGIMGYYYALNDGE
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHEEEECCCC
EAVVATALNEQYMPRFSGDDLPSTDLGKALSLADRMDTLVGIFAIGQKPTGVKDPFKLRR
CEEHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHH
HALAVVRLLISISAPLNLSTLIKEALANYGDRLPQDKNLVAELKPFILERLQSYYQNQGI
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
SADLVHAVRARQDDWLYDLDKRLFALKSFITMPEATSLSAACKRVGNILAQAAYADKKVD
CHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
IKEELIEEGAEKALFDHLNKITKTVETLYIAGDYQALLKLLASLKEPVDAFFDHVMVMVE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EESLKQNRLALLQRLQELLQGVADISML
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA