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The map label for this gene is alaS [H]

Identifier: 148359345

GI number: 148359345

Start: 2088036

End: 2090618

Strand: Reverse

Name: alaS [H]

Synonym: LPC_1243

Alternate gene names: 148359345

Gene position: 2090618-2088036 (Counterclockwise)

Preceding gene: 148359346

Following gene: 148359344

Centisome position: 58.45

GC content: 38.87

Gene sequence:

>2583_bases
ATGAAAAGTTCTGAAATTAGACAAGCATTCCTTAACTATTTTGTTCAACGCGGTCATCAAATAGTAGCTTCTAGCTCATT
GGTCCCTTCAAATGATCCCACTTTACTATTTACCAATGCAGGTATGGTGCAATTCAAGGATCTTTTTTTAGGTCTTGAAA
CTCGTTCATATCAGCGTGCTGCTACAGCACAGCGCTGTGTGCGTGCAGGTGGTAAACATAATGATCTGGAAAATGTTGGT
TATACGGCGAGACATCATACATTTTTTGAAATGTTGGGTAATTTTAGTTTTGGTGATTATTTTAAACGCGAAGCTATTCA
ATATGCTTGGGAATTCCTAACAGAAGTTTTACACATACCGGCAGAACGGCTATGGGTTACTGTCTATAAAGAGGATTTGG
AAGCAGAAGACATATGGCTAAAGGAAATGAAGGTATCTCCCGAGCGATTCTCTCGATGTGGGGAAAAAGATAATTTCTGG
TCAATGGGGGATACGGGTCCATGTGGTCCTTGCACAGAAATTTTTTATGATCATGGTCCTGAAGTAGCAGGTGGTCCCCC
GGGCAGTCCAGATGAAGATGGAGATCGTTATATAGAAATATGGAACCTGGTATTCATGCAGTTTAACAGGGACAGAGAGG
GACATTTGCACCCCTTGCCAAAGCCTTCTGTGGATACTGGTATGGGATTGGAACGATTAGCCGCAGTTATTCAAGGTGTC
CATAGTAATTATGAAATTGATAGTTTTCAGTATTTGATTAAAGCAATAGCACAATTGGGCCAGGATATTGATTTAAATCA
TACTTCCTTAAAAGTGATTGCAGATCATATTCGTTCCTGCTCTTTTTTGATAGTTGATGGGGTATTGCCTAGTAATGAAG
GCAGAGGATATGTATTAAGAAGGATTATCCGCAGAGCAGTCAGACATGGGAATAAATTAGGCTTACCCAGCCCATTTTTT
TCCAAACTTGTTCAGCCTTTGATTGATGTTATGGGGGATGCTTATCCCGAGCTAATCAATTCGAAAGCGCATATTGAAAG
GATCTTGCAACAAGAAGAAAATCAGTTTACCCGTACTTTGGAACAAGGATTGCGTTTATTACAAGATCATATTAAAAATT
TGCAAGGCCAGGAACTATCAGGAGAAGTGGCATTCAAGTTGTATGATACTTATGGTTTCCCTATCGATTTGACAGCAGAC
ATCATTCGTGAACAAGGTTTACATATTGATATGGAAGCATTTAATCAATTGATGCAACAACAAAGAGAACAGTCTCAAGC
CGCCAGTCAGTTTACTACAGATTATCACGCCGTGTCTCAATTGGATCATCAGTCTGAATTTCATGGGTATGAAAAAGAAT
CCATGGAAGCAAAGATTATTGGATTACTTCAAGAAGGTAATGAAGTGAAATCTATTAATAAGGGAGCAAAAGGGGCTGTA
ATTCTTGATCATACCCCATTTTATGCTGAAAGTGGTGGTCAGGTAGGAGATAAAGGATTACTCATAGGAAAAGAATTTAC
TTTTCAAGTCGATGATACACAAAAAGTAGGGCAAGCTGTCGTACATTATGGAAAAGTAATTAAAGGTGAGCTCACTTTAG
ATTTATTAATCCATGCGCAAGTAGATAACATAAGACGGGATGCAATTCGATTGAATCATACCGCAACTCATTTGTTACAT
GCTGCTCTTAAAAAAATTGTAGGGCAACATGTTCAACAACGAGGATCCCTGGTGGATGCTGAAAGGGCACGTTTTGACTT
TTCTCATTTCGAGGCATTGACTCCTCAACAAATTCAGCAAATTGAAGAGGTAGTTAATGCACAGATCCGAGCTAATAATG
AAGTAATAACTCAAGTAATGGACATTGAATCAGCCAAGCAAAGTGGTGCTGTTGCTTTATTCGGGGAAAAATACTCCGAC
GCAGTACGCGTATTATCCATGGGTGATTTTTCCAAGGAGCTTTGTGGAGGAACTCATGCACGACGTACTGGTGATATAGG
TCTTTTCAAAATCGTAGCAGAATATGGTATTGCCAGCGGTATCAGGCGGATTGAGATGGTTACGGGTCGTTATGCATTAG
CCTGGGTCAATGAACAGCTGGGATTTATGAATAATCTGGCAGCTACCCTTAAAACAACACCTAATAGTCTACAGGAAAAA
GTTTCTCAATTATTGCTTGATAATAAAAATCAAGAAAAAATGATTGCAAAACTTTTAAGTGAAAAAGCACAAAAATCTGG
AGCAGATATTCTTGGTGAGATCGAGGAGATCAAGGGTATTAATTTATTGATTAAGCAGTTAGAGGGTATGGATAGCCAAA
CCATGCGCCATACCATGGATCAATTAAAATCAAGAATTGATTCAGCGGTGATCATATTATTTACTATTGAACAGAATAAA
ATGAATGTCATTGCCGGGGTGAGTAAGAATATTATTGGCAAAGCACCAAGTGCTGCTCAATTAGTAAGACATCTTTGTGG
TAAAGGAGGTGGTCGAGATGATATGGCTCAAGGTGGTGGGGGTGTTCCTGAAGATTTGAACAGTAAGATCAAAGAAATAA
AGGAAATGATTGAAAAAATTTAA

Upstream 100 bases:

>100_bases
AACGGTTTTTGCTGTATCGAGGGTTTGATAGGGATGTTATTTCAAAGGTATTTAAAGAGGTTAAATCTTCTTACTTAATT
TAACTGGTAAATATTAAAAA

Downstream 100 bases:

>100_bases
TATTTCCAGATTTTTCTTTTCGACTGCAGAAAAGCAAGTTGTTTTTGGATTTGCTATAATATAGACACTGGTTTACTAAT
GAGCACTATGATGAATGACT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 860; Mature: 860

Protein sequence:

>860_residues
MKSSEIRQAFLNYFVQRGHQIVASSSLVPSNDPTLLFTNAGMVQFKDLFLGLETRSYQRAATAQRCVRAGGKHNDLENVG
YTARHHTFFEMLGNFSFGDYFKREAIQYAWEFLTEVLHIPAERLWVTVYKEDLEAEDIWLKEMKVSPERFSRCGEKDNFW
SMGDTGPCGPCTEIFYDHGPEVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGHLHPLPKPSVDTGMGLERLAAVIQGV
HSNYEIDSFQYLIKAIAQLGQDIDLNHTSLKVIADHIRSCSFLIVDGVLPSNEGRGYVLRRIIRRAVRHGNKLGLPSPFF
SKLVQPLIDVMGDAYPELINSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLQGQELSGEVAFKLYDTYGFPIDLTAD
IIREQGLHIDMEAFNQLMQQQREQSQAASQFTTDYHAVSQLDHQSEFHGYEKESMEAKIIGLLQEGNEVKSINKGAKGAV
ILDHTPFYAESGGQVGDKGLLIGKEFTFQVDDTQKVGQAVVHYGKVIKGELTLDLLIHAQVDNIRRDAIRLNHTATHLLH
AALKKIVGQHVQQRGSLVDAERARFDFSHFEALTPQQIQQIEEVVNAQIRANNEVITQVMDIESAKQSGAVALFGEKYSD
AVRVLSMGDFSKELCGGTHARRTGDIGLFKIVAEYGIASGIRRIEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEK
VSQLLLDNKNQEKMIAKLLSEKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMDQLKSRIDSAVIILFTIEQNK
MNVIAGVSKNIIGKAPSAAQLVRHLCGKGGGRDDMAQGGGGVPEDLNSKIKEIKEMIEKI

Sequences:

>Translated_860_residues
MKSSEIRQAFLNYFVQRGHQIVASSSLVPSNDPTLLFTNAGMVQFKDLFLGLETRSYQRAATAQRCVRAGGKHNDLENVG
YTARHHTFFEMLGNFSFGDYFKREAIQYAWEFLTEVLHIPAERLWVTVYKEDLEAEDIWLKEMKVSPERFSRCGEKDNFW
SMGDTGPCGPCTEIFYDHGPEVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGHLHPLPKPSVDTGMGLERLAAVIQGV
HSNYEIDSFQYLIKAIAQLGQDIDLNHTSLKVIADHIRSCSFLIVDGVLPSNEGRGYVLRRIIRRAVRHGNKLGLPSPFF
SKLVQPLIDVMGDAYPELINSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLQGQELSGEVAFKLYDTYGFPIDLTAD
IIREQGLHIDMEAFNQLMQQQREQSQAASQFTTDYHAVSQLDHQSEFHGYEKESMEAKIIGLLQEGNEVKSINKGAKGAV
ILDHTPFYAESGGQVGDKGLLIGKEFTFQVDDTQKVGQAVVHYGKVIKGELTLDLLIHAQVDNIRRDAIRLNHTATHLLH
AALKKIVGQHVQQRGSLVDAERARFDFSHFEALTPQQIQQIEEVVNAQIRANNEVITQVMDIESAKQSGAVALFGEKYSD
AVRVLSMGDFSKELCGGTHARRTGDIGLFKIVAEYGIASGIRRIEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEK
VSQLLLDNKNQEKMIAKLLSEKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMDQLKSRIDSAVIILFTIEQNK
MNVIAGVSKNIIGKAPSAAQLVRHLCGKGGGRDDMAQGGGGVPEDLNSKIKEIKEMIEKI
>Mature_860_residues
MKSSEIRQAFLNYFVQRGHQIVASSSLVPSNDPTLLFTNAGMVQFKDLFLGLETRSYQRAATAQRCVRAGGKHNDLENVG
YTARHHTFFEMLGNFSFGDYFKREAIQYAWEFLTEVLHIPAERLWVTVYKEDLEAEDIWLKEMKVSPERFSRCGEKDNFW
SMGDTGPCGPCTEIFYDHGPEVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGHLHPLPKPSVDTGMGLERLAAVIQGV
HSNYEIDSFQYLIKAIAQLGQDIDLNHTSLKVIADHIRSCSFLIVDGVLPSNEGRGYVLRRIIRRAVRHGNKLGLPSPFF
SKLVQPLIDVMGDAYPELINSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLQGQELSGEVAFKLYDTYGFPIDLTAD
IIREQGLHIDMEAFNQLMQQQREQSQAASQFTTDYHAVSQLDHQSEFHGYEKESMEAKIIGLLQEGNEVKSINKGAKGAV
ILDHTPFYAESGGQVGDKGLLIGKEFTFQVDDTQKVGQAVVHYGKVIKGELTLDLLIHAQVDNIRRDAIRLNHTATHLLH
AALKKIVGQHVQQRGSLVDAERARFDFSHFEALTPQQIQQIEEVVNAQIRANNEVITQVMDIESAKQSGAVALFGEKYSD
AVRVLSMGDFSKELCGGTHARRTGDIGLFKIVAEYGIASGIRRIEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEK
VSQLLLDNKNQEKMIAKLLSEKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMDQLKSRIDSAVIILFTIEQNK
MNVIAGVSKNIIGKAPSAAQLVRHLCGKGGGRDDMAQGGGGVPEDLNSKIKEIKEMIEKI

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=953, Percent_Identity=37.4606505771249, Blast_Score=546, Evalue=1e-155,
Organism=Homo sapiens, GI38569417, Length=888, Percent_Identity=35.9234234234234, Blast_Score=481, Evalue=1e-135,
Organism=Escherichia coli, GI1789048, Length=850, Percent_Identity=57.0588235294118, Blast_Score=1021, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=969, Percent_Identity=36.2229102167183, Blast_Score=541, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17536681, Length=778, Percent_Identity=34.0616966580977, Blast_Score=416, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6324911, Length=779, Percent_Identity=41.7201540436457, Blast_Score=570, Evalue=1e-163,
Organism=Drosophila melanogaster, GI24582809, Length=966, Percent_Identity=36.3354037267081, Blast_Score=564, Evalue=1e-161,
Organism=Drosophila melanogaster, GI45552267, Length=966, Percent_Identity=36.3354037267081, Blast_Score=564, Evalue=1e-161,
Organism=Drosophila melanogaster, GI24658214, Length=777, Percent_Identity=31.4028314028314, Blast_Score=335, Evalue=6e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 96259; Mature: 96259

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSEIRQAFLNYFVQRGHQIVASSSLVPSNDPTLLFTNAGMVQFKDLFLGLETRSYQRA
CCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHH
ATAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIQYAWEFLTEVLHIP
HHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
AERLWVTVYKEDLEAEDIWLKEMKVSPERFSRCGEKDNFWSMGDTGPCGPCTEIFYDHGP
HHHHEEEEEHHCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCC
EVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGHLHPLPKPSVDTGMGLERLAAVIQGV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
HSNYEIDSFQYLIKAIAQLGQDIDLNHTSLKVIADHIRSCSFLIVDGVLPSNEGRGYVLR
HCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
RIIRRAVRHGNKLGLPSPFFSKLVQPLIDVMGDAYPELINSKAHIERILQQEENQFTRTL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
EQGLRLLQDHIKNLQGQELSGEVAFKLYDTYGFPIDLTADIIREQGLHIDMEAFNQLMQQ
HHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCEEEHHHHHHHHHH
QREQSQAASQFTTDYHAVSQLDHQSEFHGYEKESMEAKIIGLLQEGNEVKSINKGAKGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCHHHHHCCCCCCCE
ILDHTPFYAESGGQVGDKGLLIGKEFTFQVDDTQKVGQAVVHYGKVIKGELTLDLLIHAQ
EEECCCCCCCCCCCCCCCCEEEECEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEEEHH
VDNIRRDAIRLNHTATHLLHAALKKIVGQHVQQRGSLVDAERARFDFSHFEALTPQQIQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHCCCHHHHHH
IEEVVNAQIRANNEVITQVMDIESAKQSGAVALFGEKYSDAVRVLSMGDFSKELCGGTHA
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCHHHHHHCCCCC
RRTGDIGLFKIVAEYGIASGIRRIEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHH
VSQLLLDNKNQEKMIAKLLSEKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMD
HHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QLKSRIDSAVIILFTIEQNKMNVIAGVSKNIIGKAPSAAQLVRHLCGKGGGRDDMAQGGG
HHHHHCCCEEEEEEEECCCCCCEECCCCHHHCCCCCCHHHHHHHHHCCCCCCCCHHCCCC
GVPEDLNSKIKEIKEMIEKI
CCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKSSEIRQAFLNYFVQRGHQIVASSSLVPSNDPTLLFTNAGMVQFKDLFLGLETRSYQRA
CCHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCEEEEECCCHHHHHHHHHHCCHHHHHHH
ATAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIQYAWEFLTEVLHIP
HHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
AERLWVTVYKEDLEAEDIWLKEMKVSPERFSRCGEKDNFWSMGDTGPCGPCTEIFYDHGP
HHHHEEEEEHHCCCHHHHHHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCC
EVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGHLHPLPKPSVDTGMGLERLAAVIQGV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
HSNYEIDSFQYLIKAIAQLGQDIDLNHTSLKVIADHIRSCSFLIVDGVLPSNEGRGYVLR
HCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
RIIRRAVRHGNKLGLPSPFFSKLVQPLIDVMGDAYPELINSKAHIERILQQEENQFTRTL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
EQGLRLLQDHIKNLQGQELSGEVAFKLYDTYGFPIDLTADIIREQGLHIDMEAFNQLMQQ
HHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCEEEHHHHHHHHHH
QREQSQAASQFTTDYHAVSQLDHQSEFHGYEKESMEAKIIGLLQEGNEVKSINKGAKGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCHHHHHCCCCCCCE
ILDHTPFYAESGGQVGDKGLLIGKEFTFQVDDTQKVGQAVVHYGKVIKGELTLDLLIHAQ
EEECCCCCCCCCCCCCCCCEEEECEEEEEECCHHHHHHHHHHHHHHHCCCEEEEEEEEHH
VDNIRRDAIRLNHTATHLLHAALKKIVGQHVQQRGSLVDAERARFDFSHFEALTPQQIQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHHCCCHHHHHH
IEEVVNAQIRANNEVITQVMDIESAKQSGAVALFGEKYSDAVRVLSMGDFSKELCGGTHA
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCHHHHHHCCCCC
RRTGDIGLFKIVAEYGIASGIRRIEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHH
VSQLLLDNKNQEKMIAKLLSEKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMD
HHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QLKSRIDSAVIILFTIEQNKMNVIAGVSKNIIGKAPSAAQLVRHLCGKGGGRDDMAQGGG
HHHHHCCCEEEEEEEECCCCCCEECCCCHHHCCCCCCHHHHHHHHHCCCCCCCCHHCCCC
GVPEDLNSKIKEIKEMIEKI
CCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA