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The map label for this gene is fliA [H]

Identifier: 148359326

GI number: 148359326

Start: 2070340

End: 2071056

Strand: Reverse

Name: fliA [H]

Synonym: LPC_1223

Alternate gene names: 148359326

Gene position: 2071056-2070340 (Counterclockwise)

Preceding gene: 148359327

Following gene: 148359325

Centisome position: 57.91

GC content: 41.98

Gene sequence:

>717_bases
GTGGATGCTTTGGCTGCATACAGCAAAGTAAATCAACAAACCCAGGAAGCTCTGGTAAAAACACATGCCACATTGGTAAA
ACGCATTGCACATCATCTGCTCGGGCGATTACCGCAGAGTGTACAACTGGATGACTTGATACAATCAGGGATGCTCGGTC
TGCTTGAAGCAGCAAGACATTATGATTCATCCAAGGGGGCATCCTTTGAAACTTATGCAGGAATACGTATCAGGGGACAT
ATGCTTGATGAAGTAAGACGTAATGATTGGGTGCCGCGTTCTGTTTATCGCAATTCCAGAATGATTTCTGATGCTGTGCG
CATATTGGAGCATAAACTTGGGCGTGAGGCTAAGGATAATGAAATTGCTGAAGAGTTGGGTGTGTCATTAAATGATTATC
ATGATATGTTGCAAGATTCCATTAGCAGCCATTTATATGGTTTTGATGACTTGGGAGTAACCGATGATATCTTATTTGAT
GATGAAGGGAATGGCTCAACTGAACCGCACAGGAATGTTATGCGAAGTGATATGATGAATCGATTAACCCAAGTGATTGA
TAGTCTCCCTCGTAAAGAGAGGCTGGTGCTTTCTTTGTATTATGAGCAGGATTTGAATTTGAAGGAAATCGGGGAAATAC
TGGAGGTTAGTGAGTCACGAATTTCACAGATTCTAAGTCAAGCAACGCATCGCATTAGATCAAGATTACCGGAATAA

Upstream 100 bases:

>100_bases
TTAAATTATTCGCTATACTTAAAATTAGCTGTACTCTGTTTGTTTTAGATTTACCAAATTACTAATAACTACAATCTGCT
GTTATTGTAAGGAGAGTATC

Downstream 100 bases:

>100_bases
AAAATAAGTATGGATACTTTGACTTTAATAGGATTGTTGACAGGTTTTCTCGCCATTATCGTAGGCCAAATATTTGAGGG
GGGAGATTTAAATTCTTTAC

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLDDLIQSGMLGLLEAARHYDSSKGASFETYAGIRIRGH
MLDEVRRNDWVPRSVYRNSRMISDAVRILEHKLGREAKDNEIAEELGVSLNDYHDMLQDSISSHLYGFDDLGVTDDILFD
DEGNGSTEPHRNVMRSDMMNRLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSESRISQILSQATHRIRSRLPE

Sequences:

>Translated_238_residues
MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLDDLIQSGMLGLLEAARHYDSSKGASFETYAGIRIRGH
MLDEVRRNDWVPRSVYRNSRMISDAVRILEHKLGREAKDNEIAEELGVSLNDYHDMLQDSISSHLYGFDDLGVTDDILFD
DEGNGSTEPHRNVMRSDMMNRLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSESRISQILSQATHRIRSRLPE
>Mature_238_residues
MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLDDLIQSGMLGLLEAARHYDSSKGASFETYAGIRIRGH
MLDEVRRNDWVPRSVYRNSRMISDAVRILEHKLGREAKDNEIAEELGVSLNDYHDMLQDSISSHLYGFDDLGVTDDILFD
DEGNGSTEPHRNVMRSDMMNRLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSESRISQILSQATHRIRSRLPE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=224, Percent_Identity=45.9821428571429, Blast_Score=200, Evalue=6e-53,
Organism=Escherichia coli, GI1789098, Length=229, Percent_Identity=28.82096069869, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27167; Mature: 27167

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLDDLIQSGMLGLLEAARH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YDSSKGASFETYAGIRIRGHMLDEVRRNDWVPRSVYRNSRMISDAVRILEHKLGREAKDN
CCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EIAEELGVSLNDYHDMLQDSISSHLYGFDDLGVTDDILFDDEGNGSTEPHRNVMRSDMMN
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHH
RLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSESRISQILSQATHRIRSRLPE
HHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLDDLIQSGMLGLLEAARH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YDSSKGASFETYAGIRIRGHMLDEVRRNDWVPRSVYRNSRMISDAVRILEHKLGREAKDN
CCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EIAEELGVSLNDYHDMLQDSISSHLYGFDDLGVTDDILFDDEGNGSTEPHRNVMRSDMMN
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHH
RLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSESRISQILSQATHRIRSRLPE
HHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]