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The map label for this gene is dlpA [H]

Identifier: 148358581

GI number: 148358581

Start: 3207285

End: 3209129

Strand: Direct

Name: dlpA [H]

Synonym: LPC_0453

Alternate gene names: 148358581

Gene position: 3207285-3209129 (Clockwise)

Preceding gene: 148358582

Following gene: 148358580

Centisome position: 89.68

GC content: 40.38

Gene sequence:

>1845_bases
ATGAAATCCACTGATCCTATAAAAATTGCTGTATTACCAGGTGACGGGATTGGTATTGAAGTGACTGAGGCTACACTTCC
TGTTTTTGAAGTCCTTGATGTTCCTGTAATCTTGAATTATGGTGATATTGGCTGGGAATTTTGGAAAAAAGAAGGAGCAG
CAATCCCCTCCAGAACATGGCAATTGATAGCCTCCTCTGATACCGTTTTGCTAGGAGCGATTACCAGCAAGCCGCAACGT
GAAGCCAAACAGGAATTAAGCAACGCGCTTAAAAAGAGCAATCCTTATTATGTTTCTCCCGTCATTCAGTTAAGGCAAGG
TTTGGATCTTTTTGCGAATGTTCGTCCCTGTTTTTCCATTGATGACCAATCAAAGCCGTTTAATTTTTGTATCATTAGAG
AAAATAGCGAAGGATTGTATTGCGGATTTGATTATTTTCCATTACCAAAAGCAATACATAGTTTATTAGCAGAAAGCCAA
CATTGGCAAACGATTCCAGCTGATGAAGCAAGTTGTGCCTTAAGATTGCAATCAAAATCCGGTTTAACCCGTTTATTTGA
TTTCGCCTTTAAGCATGCCATGCAAACCGGCATGCCTAGAGTCACTTTAGCAGATAAACCAAATGTTCTTAGAGAAAGCG
GCGAATTTACCAGGAAAATTTTTGAAAGCACAGCCCAAAGATACCCGAAAATCCAGGCGGACATACTCAATGTCGATGCC
GTAGCATTATGGTTGATAAAAAGCCCGGAAAAATTTGGAGTAATCGTCGCGGAAAATATGTTTGGTGATATTTTATCCGA
CGTCGGAGCAGGAGTCATGGGGGGATTAGGCCTTGCACCCAGTGCCAATATTGGAGATAAAGGGAGTTATTTTGAGCCAG
TACATGGAAGCGGGCCAAGAATAAGAAAAAATTGTGCCAATCCATCAGCTATGTTTTTAACGATTAGCATGCTGTTAAAT
CATTTTGGTTATCCTGACCGAGCAAAAAAAATAGTCAATGCCGTAATGCAAGTCATAAAGGAAAAGCGTTTTATTACTTA
TGATCTGGGTGGGCATGCCACTACGACAGATATGGCGAATGCAGTGATTGAACATTGCGCGCGATTGAATGCCTCTTGTT
TATCAAAGGATTTTAATCCCACCCCTAAAGAAAATTTAATTGAGTCTGATACTATGCCCAACTTGCTTCAGCAATTAATG
CACTGTAATTCTGCAGAAATTTCTGACGCCCTGGACGCTTGTGGAATCGAAGGCGGATTATTGAATATCAAACCCTTATC
ACAGGGAATGAAAATGGTCGGGCCTGCTTATACTATCCAATACTTACCCAGAGAAAAAAAAGGAACAGCCTTCCATAATG
CCGCAAACTATATAGACAAGGTTCCCAAACATTCGGTAATAGTTATTGACAACAATGGTCAAATTGATTGCACCGTTTGG
GGTGACCTATTAACACATACCGCATTGAGAAATAACATCATGGGCACTGTTGTGCATGGGGCAGTGAGAGATGTCGAATC
AATTCGCTCAACAAACTATCCTGTTTTTTGTACGGGAATTTATATGTGCTCTGGTAAGAATCGCGTTTACAAAGCCAATG
AACAATGCCCTTTATCCATTAATGGAGTTACAATAAATCCTGGCGACATTATCTTTGCTGATGACAATGGAGTCTTGGTG
ATCCCAAATGATCGCCTTCAGGAGGTAGTGAATAAAACAATTGCCATAAGATTAACTGAGAAACGCATTAAAGCAGCTGT
AGCATCAGGTTCTACTCTTGAGCAAGCTCGAGAAGATTATTACTATGAACAACCCTGGTTAGGTATTAATAAAAAGAGAG
AGTAA

Upstream 100 bases:

>100_bases
CCATCATGTGGCCAATTGATTGTAGTTCCCGAAGCAAGTATGCGAGTCCTGGAAAGCAACCCCACTTTTTGCAAAGAAAT
TATATTTAAGGCCCTGAATC

Downstream 100 bases:

>100_bases
TAGTGTTTTCGCCTGAAAAACTCCAATTCATAGACATACACTACCATGCCGCCCCTGATCTGTATATTCGTCGCAGAAAT
GCAATTGAAACGGGAAGATT

Product: DlpA protein

Products: @HYDR01.txt*Oxaloglycolate!; NADH. [C]

Alternate protein names: NA

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MKSTDPIKIAVLPGDGIGIEVTEATLPVFEVLDVPVILNYGDIGWEFWKKEGAAIPSRTWQLIASSDTVLLGAITSKPQR
EAKQELSNALKKSNPYYVSPVIQLRQGLDLFANVRPCFSIDDQSKPFNFCIIRENSEGLYCGFDYFPLPKAIHSLLAESQ
HWQTIPADEASCALRLQSKSGLTRLFDFAFKHAMQTGMPRVTLADKPNVLRESGEFTRKIFESTAQRYPKIQADILNVDA
VALWLIKSPEKFGVIVAENMFGDILSDVGAGVMGGLGLAPSANIGDKGSYFEPVHGSGPRIRKNCANPSAMFLTISMLLN
HFGYPDRAKKIVNAVMQVIKEKRFITYDLGGHATTTDMANAVIEHCARLNASCLSKDFNPTPKENLIESDTMPNLLQQLM
HCNSAEISDALDACGIEGGLLNIKPLSQGMKMVGPAYTIQYLPREKKGTAFHNAANYIDKVPKHSVIVIDNNGQIDCTVW
GDLLTHTALRNNIMGTVVHGAVRDVESIRSTNYPVFCTGIYMCSGKNRVYKANEQCPLSINGVTINPGDIIFADDNGVLV
IPNDRLQEVVNKTIAIRLTEKRIKAAVASGSTLEQAREDYYYEQPWLGINKKRE

Sequences:

>Translated_614_residues
MKSTDPIKIAVLPGDGIGIEVTEATLPVFEVLDVPVILNYGDIGWEFWKKEGAAIPSRTWQLIASSDTVLLGAITSKPQR
EAKQELSNALKKSNPYYVSPVIQLRQGLDLFANVRPCFSIDDQSKPFNFCIIRENSEGLYCGFDYFPLPKAIHSLLAESQ
HWQTIPADEASCALRLQSKSGLTRLFDFAFKHAMQTGMPRVTLADKPNVLRESGEFTRKIFESTAQRYPKIQADILNVDA
VALWLIKSPEKFGVIVAENMFGDILSDVGAGVMGGLGLAPSANIGDKGSYFEPVHGSGPRIRKNCANPSAMFLTISMLLN
HFGYPDRAKKIVNAVMQVIKEKRFITYDLGGHATTTDMANAVIEHCARLNASCLSKDFNPTPKENLIESDTMPNLLQQLM
HCNSAEISDALDACGIEGGLLNIKPLSQGMKMVGPAYTIQYLPREKKGTAFHNAANYIDKVPKHSVIVIDNNGQIDCTVW
GDLLTHTALRNNIMGTVVHGAVRDVESIRSTNYPVFCTGIYMCSGKNRVYKANEQCPLSINGVTINPGDIIFADDNGVLV
IPNDRLQEVVNKTIAIRLTEKRIKAAVASGSTLEQAREDYYYEQPWLGINKKRE
>Mature_614_residues
MKSTDPIKIAVLPGDGIGIEVTEATLPVFEVLDVPVILNYGDIGWEFWKKEGAAIPSRTWQLIASSDTVLLGAITSKPQR
EAKQELSNALKKSNPYYVSPVIQLRQGLDLFANVRPCFSIDDQSKPFNFCIIRENSEGLYCGFDYFPLPKAIHSLLAESQ
HWQTIPADEASCALRLQSKSGLTRLFDFAFKHAMQTGMPRVTLADKPNVLRESGEFTRKIFESTAQRYPKIQADILNVDA
VALWLIKSPEKFGVIVAENMFGDILSDVGAGVMGGLGLAPSANIGDKGSYFEPVHGSGPRIRKNCANPSAMFLTISMLLN
HFGYPDRAKKIVNAVMQVIKEKRFITYDLGGHATTTDMANAVIEHCARLNASCLSKDFNPTPKENLIESDTMPNLLQQLM
HCNSAEISDALDACGIEGGLLNIKPLSQGMKMVGPAYTIQYLPREKKGTAFHNAANYIDKVPKHSVIVIDNNGQIDCTVW
GDLLTHTALRNNIMGTVVHGAVRDVESIRSTNYPVFCTGIYMCSGKNRVYKANEQCPLSINGVTINPGDIIFADDNGVLV
IPNDRLQEVVNKTIAIRLTEKRIKAAVASGSTLEQAREDYYYEQPWLGINKKRE

Specific function: Unknown

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: To M.jannaschii MJ0644 in the C-terminal section [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=367, Percent_Identity=32.6975476839237, Blast_Score=182, Evalue=5e-46,
Organism=Homo sapiens, GI4758582, Length=371, Percent_Identity=28.0323450134771, Blast_Score=137, Evalue=4e-32,
Organism=Homo sapiens, GI28178821, Length=365, Percent_Identity=28.2191780821918, Blast_Score=136, Evalue=6e-32,
Organism=Homo sapiens, GI28178816, Length=363, Percent_Identity=28.099173553719, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI28178838, Length=333, Percent_Identity=28.2282282282282, Blast_Score=120, Evalue=5e-27,
Organism=Homo sapiens, GI28178819, Length=223, Percent_Identity=30.4932735426009, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1788101, Length=380, Percent_Identity=28.1578947368421, Blast_Score=142, Evalue=8e-35,
Organism=Escherichia coli, GI87081683, Length=374, Percent_Identity=29.9465240641711, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1787381, Length=385, Percent_Identity=26.2337662337662, Blast_Score=112, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI71986051, Length=371, Percent_Identity=31.266846361186, Blast_Score=183, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17550882, Length=365, Percent_Identity=28.4931506849315, Blast_Score=164, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17505779, Length=366, Percent_Identity=25.4098360655738, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI25144293, Length=362, Percent_Identity=27.0718232044199, Blast_Score=130, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6322097, Length=370, Percent_Identity=31.8918918918919, Blast_Score=175, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6324709, Length=373, Percent_Identity=31.0991957104558, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6324291, Length=371, Percent_Identity=27.7628032345013, Blast_Score=150, Evalue=5e-37,
Organism=Saccharomyces cerevisiae, GI6319830, Length=391, Percent_Identity=29.156010230179, Blast_Score=131, Evalue=2e-31,
Organism=Saccharomyces cerevisiae, GI6320847, Length=230, Percent_Identity=31.304347826087, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24643268, Length=371, Percent_Identity=31.266846361186, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI24643270, Length=371, Percent_Identity=31.266846361186, Blast_Score=185, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24661184, Length=371, Percent_Identity=29.1105121293801, Blast_Score=164, Evalue=2e-40,
Organism=Drosophila melanogaster, GI281362242, Length=360, Percent_Identity=26.6666666666667, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24648872, Length=360, Percent_Identity=26.6666666666667, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161078637, Length=361, Percent_Identity=26.5927977839335, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161078635, Length=361, Percent_Identity=26.5927977839335, Blast_Score=124, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161078633, Length=361, Percent_Identity=26.5927977839335, Blast_Score=124, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24650122, Length=361, Percent_Identity=26.5927977839335, Blast_Score=124, Evalue=3e-28,
Organism=Drosophila melanogaster, GI161078639, Length=359, Percent_Identity=26.4623955431755, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI20130355, Length=363, Percent_Identity=23.1404958677686, Blast_Score=108, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR005493 [H]

Pfam domain/function: PF00180 Iso_dh; PF03737 Methyltransf_6 [H]

EC number: 1.1.1.93 [C]

Molecular weight: Translated: 67705; Mature: 67705

Theoretical pI: Translated: 7.08; Mature: 7.08

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSTDPIKIAVLPGDGIGIEVTEATLPVFEVLDVPVILNYGDIGWEFWKKEGAAIPSRTW
CCCCCCEEEEEECCCCCCEEEECCCCCHHHHHCCCEEEECCCCCHHHHHHCCCCCCCCCE
QLIASSDTVLLGAITSKPQREAKQELSNALKKSNPYYVSPVIQLRQGLDLFANVRPCFSI
EEEECCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEECHHHHHHCCCHHHHCCCCCCCC
DDQSKPFNFCIIRENSEGLYCGFDYFPLPKAIHSLLAESQHWQTIPADEASCALRLQSKS
CCCCCCEEEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCC
GLTRLFDFAFKHAMQTGMPRVTLADKPNVLRESGEFTRKIFESTAQRYPKIQADILNVDA
CHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHCHHHHHHHHHHHHHHCCCCCHHEECCCE
VALWLIKSPEKFGVIVAENMFGDILSDVGAGVMGGLGLAPSANIGDKGSYFEPVHGSGPR
EEEEEEECCCCCEEEEEHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
IRKNCANPSAMFLTISMLLNHFGYPDRAKKIVNAVMQVIKEKRFITYDLGGHATTTDMAN
HHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHH
AVIEHCARLNASCLSKDFNPTPKENLIESDTMPNLLQQLMHCNSAEISDALDACGIEGGL
HHHHHHHHCCHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCE
LNIKPLSQGMKMVGPAYTIQYLPREKKGTAFHNAANYIDKVPKHSVIVIDNNGQIDCTVW
EEEHHHHCCHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEEEH
GDLLTHTALRNNIMGTVVHGAVRDVESIRSTNYPVFCTGIYMCSGKNRVYKANEQCPLSI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEECCCCCCEEE
NGVTINPGDIIFADDNGVLVIPNDRLQEVVNKTIAIRLTEKRIKAAVASGSTLEQAREDY
CCEEECCCCEEEECCCCEEEECCHHHHHHHCCEEEEEEEHHHHHHHHHCCCHHHHHHHHC
YYEQPWLGINKKRE
CCCCCCCCCCCCCC
>Mature Secondary Structure
MKSTDPIKIAVLPGDGIGIEVTEATLPVFEVLDVPVILNYGDIGWEFWKKEGAAIPSRTW
CCCCCCEEEEEECCCCCCEEEECCCCCHHHHHCCCEEEECCCCCHHHHHHCCCCCCCCCE
QLIASSDTVLLGAITSKPQREAKQELSNALKKSNPYYVSPVIQLRQGLDLFANVRPCFSI
EEEECCCEEEEEECCCCHHHHHHHHHHHHHHCCCCEEECHHHHHHCCCHHHHCCCCCCCC
DDQSKPFNFCIIRENSEGLYCGFDYFPLPKAIHSLLAESQHWQTIPADEASCALRLQSKS
CCCCCCEEEEEEEECCCCEEECCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCC
GLTRLFDFAFKHAMQTGMPRVTLADKPNVLRESGEFTRKIFESTAQRYPKIQADILNVDA
CHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHCHHHHHHHHHHHHHHCCCCCHHEECCCE
VALWLIKSPEKFGVIVAENMFGDILSDVGAGVMGGLGLAPSANIGDKGSYFEPVHGSGPR
EEEEEEECCCCCEEEEEHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCC
IRKNCANPSAMFLTISMLLNHFGYPDRAKKIVNAVMQVIKEKRFITYDLGGHATTTDMAN
HHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHH
AVIEHCARLNASCLSKDFNPTPKENLIESDTMPNLLQQLMHCNSAEISDALDACGIEGGL
HHHHHHHHCCHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCCE
LNIKPLSQGMKMVGPAYTIQYLPREKKGTAFHNAANYIDKVPKHSVIVIDNNGQIDCTVW
EEEHHHHCCHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEEEEH
GDLLTHTALRNNIMGTVVHGAVRDVESIRSTNYPVFCTGIYMCSGKNRVYKANEQCPLSI
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCEEECCCCCCEEE
NGVTINPGDIIFADDNGVLVIPNDRLQEVVNKTIAIRLTEKRIKAAVASGSTLEQAREDY
CCEEECCCCEEEECCCCEEEECCHHHHHHHCCEEEEEEEHHHHHHHHHCCCHHHHHHHHC
YYEQPWLGINKKRE
CCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.28 {NAD}} 2.3 {L-(+)-tartrate}} [C]

Substrates: Tartrate; NAD(+) [C]

Specific reaction: Tartrate + NAD(+) = @HYDR01.txt*Oxaloglycolate! + NADH. [C]

General reaction: Redox reaction [C]

Inhibitor: ATP; Dihydroxy fumarate; meso-Tartrate; Oxaloacetate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7891566 [H]