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The map label for this gene is phrB [H]

Identifier: 148358421

GI number: 148358421

Start: 301079

End: 302494

Strand: Reverse

Name: phrB [H]

Synonym: LPC_0287

Alternate gene names: 148358421

Gene position: 302494-301079 (Counterclockwise)

Preceding gene: 148358422

Following gene: 148358420

Centisome position: 8.46

GC content: 41.53

Gene sequence:

>1416_bases
ATGACTATTGCTCTTGTGTGGTTCCGTCAAGATTTAAGATTGACTGATAATCCTGCGTTCATCGAAGCATGCTCCCATCA
TGAAATTGTAATTCCACTTTATATTTATGATGATAAATGCAGTGTTTTAGGGCAAGCTCAAGCTTGGTGGCTCCATCATA
GTCTAATTGCTTTGGGCAAATCGTTAAATCAACAGGGTTTAAGCCTCGTTCTCCGCAAAGGATCTCCTCAAGAGATTATT
TTGGAATTAGTCTCGCAGTATGGTGTGGAATCAGTCTATTGGAATCGCTGCTATGAGCCACCTGTCTTTGCACGCGATAA
AAAAATTAAGCTTGCTTTGGGCGAAAGAGAAATTAAAGCGCAGAGTTTCAATGGAAATTTACTTCATGAGCCGTGGGCAA
TTAAAAATAAAAATGGGGATTTTTTTAAGGTTTTTACTCCTTACTGGAAACAATGTCGACAAATTCTTAATATCCCCTCG
CCTCTTATGCTCACTAAACGCCCAAGGGGAATCGAAATTACAAGTGATGACATTGACAAATGGAAGTTATTGCCAGCAAT
TAATTGGGCAGCACGATTTTCTGAATATTGGACTCCTGGAGAAAAGGGCGCACAAAAAAAACTGGATGAGTTTATTATTC
ACCACCTTAGCGGATATAAAAGAGATCGCGATTTTCCAGCAAAAAATGCGACATCTCGATTATCACCTCATTTGCACTTT
GGTGAAATCAGCCCTTGGGTGATTTTAAGAGCGCTGGAGTTGACTAAACTGGAACAAACCTGTGATCTGGCATCCGTAGA
GCATTTTTTATCAGAGCTGGGATGGAGAGAGTTTTCTGTCTATCTGCTCTACCACTTCCCCAAGCTGGATTGCGAAAATT
TTAGAAATGAATTTGATGCATTCCCCTGGCAAAATGATGAGCAATTATTAACCCGTTGGCAAAAGGGAATGACTGGGTAT
CCTCTCATTGATGCTGGAATGAGAGAATTATGGGCCACAGGATACATGCACAACCGCGTTCGTATGATTGTCGCATCATT
TCTTACCAAAGATTTACTGATTGATTGGCGTTTGGGTGCACAGTGGTTTTTAGACACCCTAGTCGATGCGGATCTGGCGA
ATAACAGCGCGAGCTGGCAATGGGTAGCAGGATGTGGTGCGGATGCAGCACCTTATTTCCGTATTTTCAATCCTGTTTTA
CAAAGCCAAAAATTTGACCCGGATGGCGTTTACATACGCCAATGGATCCCCGAACTTGCCCAATTAAATAGTCAGTCAAT
TCATCAACCATGGACAGATGCCAGCGCCAGTTCCATTTTTTTAGGAAACAAATATCCCAAACCCATCATTAATCACCAGG
AAGCGAGAACGAAGGCATTGAGTTACTATCAGCAAATAAAAAAAGGAATGGATTGA

Upstream 100 bases:

>100_bases
AATAAAAGTGCGTTCAGTAGAAGTTAAGAAGGAACCCTCTAGTTAAGAAAAAATCATAAGCATGTTATTTTATTGGTTTT
TCCATCAATAGAAGAAAAGA

Downstream 100 bases:

>100_bases
CAAGAGACTAACTAAAATTTGGGGAGATCCTATTGAACAAAAAAAGTTGGATGGAACTGATTGCCTGGATCATTACCTTT
GAAGCAATCGGATTTTTTTT

Product: deoxyribodipyrimidine photolyase

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MTIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHHSLIALGKSLNQQGLSLVLRKGSPQEII
LELVSQYGVESVYWNRCYEPPVFARDKKIKLALGEREIKAQSFNGNLLHEPWAIKNKNGDFFKVFTPYWKQCRQILNIPS
PLMLTKRPRGIEITSDDIDKWKLLPAINWAARFSEYWTPGEKGAQKKLDEFIIHHLSGYKRDRDFPAKNATSRLSPHLHF
GEISPWVILRALELTKLEQTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRNEFDAFPWQNDEQLLTRWQKGMTGY
PLIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRLGAQWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPVL
QSQKFDPDGVYIRQWIPELAQLNSQSIHQPWTDASASSIFLGNKYPKPIINHQEARTKALSYYQQIKKGMD

Sequences:

>Translated_471_residues
MTIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHHSLIALGKSLNQQGLSLVLRKGSPQEII
LELVSQYGVESVYWNRCYEPPVFARDKKIKLALGEREIKAQSFNGNLLHEPWAIKNKNGDFFKVFTPYWKQCRQILNIPS
PLMLTKRPRGIEITSDDIDKWKLLPAINWAARFSEYWTPGEKGAQKKLDEFIIHHLSGYKRDRDFPAKNATSRLSPHLHF
GEISPWVILRALELTKLEQTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRNEFDAFPWQNDEQLLTRWQKGMTGY
PLIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRLGAQWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPVL
QSQKFDPDGVYIRQWIPELAQLNSQSIHQPWTDASASSIFLGNKYPKPIINHQEARTKALSYYQQIKKGMD
>Mature_470_residues
TIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHHSLIALGKSLNQQGLSLVLRKGSPQEIIL
ELVSQYGVESVYWNRCYEPPVFARDKKIKLALGEREIKAQSFNGNLLHEPWAIKNKNGDFFKVFTPYWKQCRQILNIPSP
LMLTKRPRGIEITSDDIDKWKLLPAINWAARFSEYWTPGEKGAQKKLDEFIIHHLSGYKRDRDFPAKNATSRLSPHLHFG
EISPWVILRALELTKLEQTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRNEFDAFPWQNDEQLLTRWQKGMTGYP
LIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRLGAQWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPVLQ
SQKFDPDGVYIRQWIPELAQLNSQSIHQPWTDASASSIFLGNKYPKPIINHQEARTKALSYYQQIKKGMD

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=493, Percent_Identity=29.0060851926978, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI4758072, Length=493, Percent_Identity=27.5862068965517, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI188536103, Length=453, Percent_Identity=28.2560706401766, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1786926, Length=486, Percent_Identity=38.2716049382716, Blast_Score=302, Evalue=4e-83,
Organism=Saccharomyces cerevisiae, GI6324962, Length=276, Percent_Identity=33.695652173913, Blast_Score=171, Evalue=2e-43,
Organism=Drosophila melanogaster, GI17137248, Length=476, Percent_Identity=27.5210084033613, Blast_Score=165, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24585455, Length=476, Percent_Identity=27.5210084033613, Blast_Score=165, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24648152, Length=515, Percent_Identity=25.631067961165, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 54707; Mature: 54576

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00639 THIOL_PROTEASE_HIS ; PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHHSLIALGK
CEEEEEEEECCCCCCCCCHHHHHCCCCCEEEEEEEECCCHHHHCCHHHHHHHHHHHHHHH
SLNQQGLSLVLRKGSPQEIILELVSQYGVESVYWNRCYEPPVFARDKKIKLALGEREIKA
HCCCCCHHEEECCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEEECCHHHEE
QSFNGNLLHEPWAIKNKNGDFFKVFTPYWKQCRQILNIPSPLMLTKRPRGIEITSDDIDK
ECCCCCCCCCCCEEECCCCCEEEECCHHHHHHHHHHCCCCCCEECCCCCCCEEECCCCCC
WKLLPAINWAARFSEYWTPGEKGAQKKLDEFIIHHLSGYKRDRDFPAKNATSRLSPHLHF
EEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCC
GEISPWVILRALELTKLEQTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRNEFDA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHCC
FPWQNDEQLLTRWQKGMTGYPLIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRLGA
CCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCH
QWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPVLQSQKFDPDGVYIRQWIPELA
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
QLNSQSIHQPWTDASASSIFLGNKYPKPIINHQEARTKALSYYQQIKKGMD
HCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIALVWFRQDLRLTDNPAFIEACSHHEIVIPLYIYDDKCSVLGQAQAWWLHHSLIALGK
EEEEEEEECCCCCCCCCHHHHHCCCCCEEEEEEEECCCHHHHCCHHHHHHHHHHHHHHH
SLNQQGLSLVLRKGSPQEIILELVSQYGVESVYWNRCYEPPVFARDKKIKLALGEREIKA
HCCCCCHHEEECCCCHHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEEECCHHHEE
QSFNGNLLHEPWAIKNKNGDFFKVFTPYWKQCRQILNIPSPLMLTKRPRGIEITSDDIDK
ECCCCCCCCCCCEEECCCCCEEEECCHHHHHHHHHHCCCCCCEECCCCCCCEEECCCCCC
WKLLPAINWAARFSEYWTPGEKGAQKKLDEFIIHHLSGYKRDRDFPAKNATSRLSPHLHF
EEECHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCC
GEISPWVILRALELTKLEQTCDLASVEHFLSELGWREFSVYLLYHFPKLDCENFRNEFDA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHCC
FPWQNDEQLLTRWQKGMTGYPLIDAGMRELWATGYMHNRVRMIVASFLTKDLLIDWRLGA
CCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCH
QWFLDTLVDADLANNSASWQWVAGCGADAAPYFRIFNPVLQSQKFDPDGVYIRQWIPELA
HHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
QLNSQSIHQPWTDASASSIFLGNKYPKPIINHQEARTKALSYYQQIKKGMD
HCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6325460; 8905232; 9278503; 2200511; 7604260 [H]