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The map label for this gene is mmsB [H]

Identifier: 148358286

GI number: 148358286

Start: 157311

End: 158207

Strand: Reverse

Name: mmsB [H]

Synonym: LPC_0148

Alternate gene names: 148358286

Gene position: 158207-157311 (Counterclockwise)

Preceding gene: 148358287

Following gene: 148358285

Centisome position: 4.42

GC content: 42.47

Gene sequence:

>897_bases
ATGGCTAATATTGGATTTGTGGGATTGGGTCATATGGGTTTTCCAATGGCCATTAACCTTTTGAAAGCTGGTCATAGTGT
CATGGGCTATGATTTGCAACAATCAGCACTTGAGCATTTTGCAGAACAAGGAGGAGCTATTGCTCATAACTTGCAGGAAT
TGGCAAAAGACAAAGATGTCATTGTTACTATGTTACAAACCGGACAGCAAGTTTTACATGTATGCCTTGGTGTCGATGGT
ATATTCAGTCAGTTAAATGCTGGCTCATTGTACATCGATTGCTCTACAATTGATGTAAAAAGCTCTCGAGAAACTCATCA
AATAGCGAAAGAAAATCATCTTCTGGTCGTCGATGCTCCCGTCTCTGGCGGCGTAGCCGGGGCCACTGCAGCTACCTTAA
CATTCATGGTCGGTGGTGAAGCGGAGGCTTATAATGCGGCGCATCCCATTCTCTCTGCCATGGGAAAAAAAATCATCCAT
ACAGGCGGTGCAGGCAGTGGGCAAGCGGCCAAAATTTGCAATAACATGATATTGGGTACCACGATGATCGCCATATCAGA
AGCGTTCATTCTGGCGAAAGAACTAGGGCTGTCTGATGGAAAATTATTTGAGGTGGTAAACAATTCATCGGGTCAATGTT
GGGCTATGAGCAAATATGTCCCTGTACCAGGAATATTGGAAAATGTTCCTGCAAATAATGATTATAAGCCAGGGTTTGCA
GCAAACATGATGTTGAAGGATTTGTTATTGAGTCAAAATACTGCGAAATCAGTACACATTGAAACACCCCTTGGCGCCAA
AACAACTGAAATTTATCAACAATTTGTCGATCAAGGCCTTGGTGATATCGATTTTTCAGCCATTATTAAACACATTGGCA
AAACACAAGAGGTGTAG

Upstream 100 bases:

>100_bases
GACGCAAAACGGTTACCAGCAAATGGCCAATAACCGAATTAAAGGATAGCAGCTTTGCCATGCCCACTCATGATTAATCA
TGCTTATTACGGAGAGATAT

Downstream 100 bases:

>100_bases
CATGCTTCCCGTTCATCTAAAGCATCCTCATTTAACACCTACAGAAGAATCTCTATCTGAATTTTGGTCTGAAATTGCCG
CACAAACCATTGACTGGATA

Product: 3-hydroxyisobutyrate dehydrogenase

Products: NA

Alternate protein names: HIBADH [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MANIGFVGLGHMGFPMAINLLKAGHSVMGYDLQQSALEHFAEQGGAIAHNLQELAKDKDVIVTMLQTGQQVLHVCLGVDG
IFSQLNAGSLYIDCSTIDVKSSRETHQIAKENHLLVVDAPVSGGVAGATAATLTFMVGGEAEAYNAAHPILSAMGKKIIH
TGGAGSGQAAKICNNMILGTTMIAISEAFILAKELGLSDGKLFEVVNNSSGQCWAMSKYVPVPGILENVPANNDYKPGFA
ANMMLKDLLLSQNTAKSVHIETPLGAKTTEIYQQFVDQGLGDIDFSAIIKHIGKTQEV

Sequences:

>Translated_298_residues
MANIGFVGLGHMGFPMAINLLKAGHSVMGYDLQQSALEHFAEQGGAIAHNLQELAKDKDVIVTMLQTGQQVLHVCLGVDG
IFSQLNAGSLYIDCSTIDVKSSRETHQIAKENHLLVVDAPVSGGVAGATAATLTFMVGGEAEAYNAAHPILSAMGKKIIH
TGGAGSGQAAKICNNMILGTTMIAISEAFILAKELGLSDGKLFEVVNNSSGQCWAMSKYVPVPGILENVPANNDYKPGFA
ANMMLKDLLLSQNTAKSVHIETPLGAKTTEIYQQFVDQGLGDIDFSAIIKHIGKTQEV
>Mature_297_residues
ANIGFVGLGHMGFPMAINLLKAGHSVMGYDLQQSALEHFAEQGGAIAHNLQELAKDKDVIVTMLQTGQQVLHVCLGVDGI
FSQLNAGSLYIDCSTIDVKSSRETHQIAKENHLLVVDAPVSGGVAGATAATLTFMVGGEAEAYNAAHPILSAMGKKIIHT
GGAGSGQAAKICNNMILGTTMIAISEAFILAKELGLSDGKLFEVVNNSSGQCWAMSKYVPVPGILENVPANNDYKPGFAA
NMMLKDLLLSQNTAKSVHIETPLGAKTTEIYQQFVDQGLGDIDFSAIIKHIGKTQEV

Specific function: D-galactarate metabolism; third step. [C]

COG id: COG2084

COG function: function code I; 3-hydroxyisobutyrate dehydrogenase and related beta-hydroxyacid dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-hydroxyisobutyrate dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI23308751, Length=289, Percent_Identity=40.1384083044983, Blast_Score=231, Evalue=7e-61,
Organism=Homo sapiens, GI40556376, Length=288, Percent_Identity=26.7361111111111, Blast_Score=108, Evalue=7e-24,
Organism=Escherichia coli, GI145693186, Length=296, Percent_Identity=32.4324324324324, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1790315, Length=293, Percent_Identity=33.7883959044369, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1786719, Length=292, Percent_Identity=30.4794520547945, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1789092, Length=290, Percent_Identity=30, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17557316, Length=296, Percent_Identity=40.2027027027027, Blast_Score=210, Evalue=6e-55,
Organism=Drosophila melanogaster, GI24655230, Length=296, Percent_Identity=42.5675675675676, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI19922568, Length=296, Percent_Identity=42.5675675675676, Blast_Score=236, Evalue=2e-62,
Organism=Drosophila melanogaster, GI28574115, Length=291, Percent_Identity=23.0240549828179, Blast_Score=81, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002204
- InterPro:   IPR015815
- InterPro:   IPR008927
- InterPro:   IPR006115
- InterPro:   IPR013328
- InterPro:   IPR011548
- InterPro:   IPR016040 [H]

Pfam domain/function: PF03446 NAD_binding_2 [H]

EC number: =1.1.1.31 [H]

Molecular weight: Translated: 31437; Mature: 31305

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00895 3_HYDROXYISOBUT_DH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIGFVGLGHMGFPMAINLLKAGHSVMGYDLQQSALEHFAEQGGAIAHNLQELAKDKDV
CCCCCEEEECCCCHHHHHHHHHHCCHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHH
IVTMLQTGQQVLHVCLGVDGIFSQLNAGSLYIDCSTIDVKSSRETHQIAKENHLLVVDAP
HHHHHHHHHHHHHHHHCHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHCCCEEEEECC
VSGGVAGATAATLTFMVGGEAEAYNAAHPILSAMGKKIIHTGGAGSGQAAKICNNMILGT
CCCCCCHHHHHEEEEEECCCCCHHHHHHHHHHHHCHHHEECCCCCCCHHHHHHHHHHHHH
TMIAISEAFILAKELGLSDGKLFEVVNNSSGQCWAMSKYVPVPGILENVPANNDYKPGFA
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCCCCHHHHCCCCCCCCCCCHH
ANMMLKDLLLSQNTAKSVHIETPLGAKTTEIYQQFVDQGLGDIDFSAIIKHIGKTQEV
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
ANIGFVGLGHMGFPMAINLLKAGHSVMGYDLQQSALEHFAEQGGAIAHNLQELAKDKDV
CCCCEEEECCCCHHHHHHHHHHCCHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHH
IVTMLQTGQQVLHVCLGVDGIFSQLNAGSLYIDCSTIDVKSSRETHQIAKENHLLVVDAP
HHHHHHHHHHHHHHHHCHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHCCCEEEEECC
VSGGVAGATAATLTFMVGGEAEAYNAAHPILSAMGKKIIHTGGAGSGQAAKICNNMILGT
CCCCCCHHHHHEEEEEECCCCCHHHHHHHHHHHHCHHHEECCCCCCCHHHHHHHHHHHHH
TMIAISEAFILAKELGLSDGKLFEVVNNSSGQCWAMSKYVPVPGILENVPANNDYKPGFA
HHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCEEEECCCCCCCHHHHCCCCCCCCCCCHH
ANMMLKDLLLSQNTAKSVHIETPLGAKTTEIYQQFVDQGLGDIDFSAIIKHIGKTQEV
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]