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The map label for this gene is spoT22 [H]

Identifier: 148284544

GI number: 148284544

Start: 940227

End: 940802

Strand: Direct

Name: spoT22 [H]

Synonym: OTBS_0962

Alternate gene names: 148284544

Gene position: 940227-940802 (Clockwise)

Preceding gene: 148284543

Following gene: 148284548

Centisome position: 44.2

GC content: 30.56

Gene sequence:

>576_bases
ATGATAGACTTTTATAGCGAAAGCTTACTAAATAAGCTGTTTGAAACCAACGTAAGATTTAATACTGAAATTGATCTTGA
TAAAGTTGAAAAAGCTATATTTTATGCCCAAAAATATCATGGTCAGCAAAAGCGAGATACTGGAGAACTATACTACACAC
ATCAATTAGAAGTAGCTTATATGGTATCAGACTACAGCTTTGAAACTGATACAATTATTACAGCAATACTACATGATACA
CTCGAAGACACAACACTAACCAAAGAAATAATAAGTCAAGAATTTGGTAATAATATTGCAGAACAGGTTTCAGACCTCAC
CAGAATTAAGGATAATCAAAAAATCAGTTCTGGAGAAATGATTCAAACATTTTATAGGCAAAATAAAATAGAACTATTAT
TAATTAAGCTTTTCGATCGATTCCATAATATTCAGACTGTATCAATAAAACCTTATGAAAAAAGACAAAAAATCGTCATT
GAAACTCAGCAAGAATTTATACCTCTTGCTCAATACCTTAAACTACCAGAGATTGCCATAGAGCTAAATAAATACTGTGA
GCTTTATGCTAGTTAG

Upstream 100 bases:

>100_bases
TCAAATTAATTCGCTTCTTTTACAGTTTTTATCTGAGATTGAAGTATATAAAAAGTTTAATTTATTGTATAATTTATTAA
AGATTATTTAGAGATGAAAA

Downstream 100 bases:

>100_bases
AATGCAAACTTTTTCTAACTAGCATACATAGCATTATATATTTGCAAAATGGAGCATTGCTGCTTGAAATACATATACTA
TCGTTCTGGATCATCAATTA

Product: spoT-like ppGpp hydrolase

Products: NA

Alternate protein names: (p)ppGpp synthase; ATP:GTP 3'-pyrophosphotransferase [H]

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MIDFYSESLLNKLFETNVRFNTEIDLDKVEKAIFYAQKYHGQQKRDTGELYYTHQLEVAYMVSDYSFETDTIITAILHDT
LEDTTLTKEIISQEFGNNIAEQVSDLTRIKDNQKISSGEMIQTFYRQNKIELLLIKLFDRFHNIQTVSIKPYEKRQKIVI
ETQQEFIPLAQYLKLPEIAIELNKYCELYAS

Sequences:

>Translated_191_residues
MIDFYSESLLNKLFETNVRFNTEIDLDKVEKAIFYAQKYHGQQKRDTGELYYTHQLEVAYMVSDYSFETDTIITAILHDT
LEDTTLTKEIISQEFGNNIAEQVSDLTRIKDNQKISSGEMIQTFYRQNKIELLLIKLFDRFHNIQTVSIKPYEKRQKIVI
ETQQEFIPLAQYLKLPEIAIELNKYCELYAS
>Mature_191_residues
MIDFYSESLLNKLFETNVRFNTEIDLDKVEKAIFYAQKYHGQQKRDTGELYYTHQLEVAYMVSDYSFETDTIITAILHDT
LEDTTLTKEIISQEFGNNIAEQVSDLTRIKDNQKISSGEMIQTFYRQNKIELLLIKLFDRFHNIQTVSIKPYEKRQKIVI
ETQQEFIPLAQYLKLPEIAIELNKYCELYAS

Specific function: Functions as a (p)ppGpp synthase. In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. Plays a role in ada

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=184, Percent_Identity=32.6086956521739, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =2.7.6.5 [H]

Molecular weight: Translated: 22589; Mature: 22589

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDFYSESLLNKLFETNVRFNTEIDLDKVEKAIFYAQKYHGQQKRDTGELYYTHQLEVAY
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEE
MVSDYSFETDTIITAILHDTLEDTTLTKEIISQEFGNNIAEQVSDLTRIKDNQKISSGEM
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHH
IQTFYRQNKIELLLIKLFDRFHNIQTVSIKPYEKRQKIVIETQQEFIPLAQYLKLPEIAI
HHHHHHCCCHHHHHHHHHHHHCCEEEEEECCHHHCCCEEEECHHHHHHHHHHHCCCHHHH
ELNKYCELYAS
HHHHHHHHHCC
>Mature Secondary Structure
MIDFYSESLLNKLFETNVRFNTEIDLDKVEKAIFYAQKYHGQQKRDTGELYYTHQLEVAY
CCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEE
MVSDYSFETDTIITAILHDTLEDTTLTKEIISQEFGNNIAEQVSDLTRIKDNQKISSGEM
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCHH
IQTFYRQNKIELLLIKLFDRFHNIQTVSIKPYEKRQKIVIETQQEFIPLAQYLKLPEIAI
HHHHHHCCCHHHHHHHHHHHHCCEEEEEECCHHHCCCEEEECHHHHHHHHHHHCCCHHHH
ELNKYCELYAS
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA