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The map label for this gene is virB4-2 [H]

Identifier: 148284540

GI number: 148284540

Start: 924051

End: 926504

Strand: Direct

Name: virB4-2 [H]

Synonym: OTBS_0953

Alternate gene names: 148284540

Gene position: 924051-926504 (Clockwise)

Preceding gene: 148284535

Following gene: 148284541

Centisome position: 43.44

GC content: 29.3

Gene sequence:

>2454_bases
ATGGGGTTTATACATAAATTTTTACATAGTAGACAAATAGATGCAAAACAGTTTGATAGCAATGCCCAGAACTTTATTCC
TATAACTTGCCACTACAATGATGAAACTTTATTAACTAAAAATGGCGAATTGATTCAAATATTACAAATAAAAGGTATTA
ATTCTGAATATACTAATAAGGATTTATTGGGTATTAGAGCAGCAATACGTAATGCTATTAAAAATCATATTAATAATAAC
AACTTTGCAGTTTGGATACATGTTGTAAGACGCTGCGATAATATTGATGACCCTACTCCTTACAGTCAAGCATTGCCAAA
TTTTATTCATAATGTTTGGAGCAAAAAAAACTACTGGCAAGATAAATTTGTTAGTACATTATATATCTCAATTGTGCATC
ATGGATGTAACTTAAATATTAGAAACTTAGATAGTTTTTTGAACTCTTTATCGTTTAAGACATTGGATAATTTTTATGAT
AAGTTCATAGCATCTGCATTAAAAAATTTATCCATGGCTATAAATAATATAATAACTGAGCTGCAACGTTATAATCCATG
CAAGTTAAGTATTAAAATTGATAATAATAAAGTATATTCTGACCTTTTAAATTTTTATAAAAAAATAATACAACTTAATA
CTGGGTTAATAGAAGTAAAAGATGTTGATTGTGCTGTACAGTTAGCCACTCATAAATATTCTTTTGGAGGAGACAAAATA
GAGGTTATACATCAACAAGAAAAAAAGTTTGCATCTATTTTAGGTATTAAGGAATATCAAGATATTAAAGATGAATTAGT
GAGTAAGTGCTTACATGCTCCAGTGGAATTTATTGCTACAGAAATTTTTTATTTTGCTGACCAAGTAAAAGCTATTAATA
GTTACTCTCAACAAAGTAAAATTATCAGTGCAAGCCGTGACAGTGAACTAGCTCTATTCAAAGGAATAGACCCAATTGTG
AATTCTGATAACACATTACCACAGTTTTGCCATCAACAAATTAGCATAATGATTATGGCTGATAGCATTGATCTGCTTGA
GAGTAGAGTAGCTTATATATCACAAAGATTAGTAAAACAAGGAATAATACATGTTCGAGAGGATATTAATCTAGCGCAAA
CATTTTGGTCTCAGTTGCCAGGTAATTTTAATTACATTAGACGTGTTTCTTATAATATTATTGATAATGTAGCTGCTTTA
GCAGCATTACATCATTATCCGGTTGGCATGCAGCATAGTCGATGGGGTAGATCTGTCACTATATTTCGAACAGAATGCGG
TACTCCTTATTTCATGAATTTTCATGATGAGAGTGCAAAAGGACATACTTGTATAATTGGTGGAGCTCAATCTGGTCGAG
CTACTGTCACTAATTTTTTATTGTCTGAAGCATCAAAATATTCTCCAACTATACTTTATTTAACTAATAGAAATAATGCT
CAAGTTTTTATAAAAGGAATAGAAGGAACATGGTACAACGGTGATTTACCATTTAATCCATTAAGCTACTTAGAAAGCTC
TGATGACTTACTAGAACTATTTAAGATAATAACAGGCAGTTATTTTAATAATTTATCTGATAGCGAAATTGAATGCCTTA
CAGAGCTAGCTATTGCAGTTATGGATATGCCACCTGAATCACGTTCTTTGATTAAAATTATCAAAGATTTTAATTTTAAG
GATTATAGTGCTGGAGCTAGTGTAAAGAAGCGTATTAAGTTATTTATTAGTGATGATAAATGCAGTAAAATTTTTAATCA
ATCTTCTACTTTAGAATTGCAAGATAATAATGTAACTGCTATAAACTTAGCTGATTATACTGATTATCAATTTAATCAAT
CTTATACACCTCAAGTAGGAGAAAAGCCGGAAATTTATCATAACAAATTACAAACTTTGCACTGTCTAAGGTCTGGAATT
ATATTTTCTTTAATAAAAAAATTCAGCTTACTAGACTCTTCAGAGCCTAAAATACTAGTTGTTGATAATATGCCTGATTT
AATAGTAGAGCAAGTGTTTATGAACTTAATTAAACAGATCACTGAACAACTATCTGAAAATAATGGAATAATTCTTTCTG
TTGTTCAAGTAAATCAGAATGATCAATTTTATTATTCTGATTTCTGGAAGAAATGGTTAAAGTTTAATAATACTAAAATT
ATACTACCAACTAATATAAGAAATTTACCTATTGCTGAAATCTGTAACCTAAGTACTAGCGAAGCTAAAAAATTTAATAC
TATTTCACCAAATTCTAGATTATTTATGGTAAAACAAGATAATAATTATGTTATTGTAGAACTTAATTTGGAAGGGTTTC
CAGCAATTAGAAAGCTTCTATCAGCTGAAGAAGAAGATTTGAATCTTTTTCATAAAATTATGCAAAAAATAGGTGATGAT
GCTCCTTCAGCTTGGTTAAGTGATGTTTATGATCAGTTTCAGAACAGTGAGTAA

Upstream 100 bases:

>100_bases
TGTAATATTTACATTTTTTATTTAATTCTTGAAATGAAAATGTAGTAAATATCATATTAAATTAATATAGTATGCTAATA
ACAATATAAGCTTATAATTT

Downstream 100 bases:

>100_bases
TGAAGCCACAACATATCTTTTTAGTTATAATTGTTGCTGTAATTAATTTTATAACCGTTGCTAATGCCACAGCTTCGATT
TGTACATGGTTTCGTGGAAG

Product: virB4 protein precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 817; Mature: 816

Protein sequence:

>817_residues
MGFIHKFLHSRQIDAKQFDSNAQNFIPITCHYNDETLLTKNGELIQILQIKGINSEYTNKDLLGIRAAIRNAIKNHINNN
NFAVWIHVVRRCDNIDDPTPYSQALPNFIHNVWSKKNYWQDKFVSTLYISIVHHGCNLNIRNLDSFLNSLSFKTLDNFYD
KFIASALKNLSMAINNIITELQRYNPCKLSIKIDNNKVYSDLLNFYKKIIQLNTGLIEVKDVDCAVQLATHKYSFGGDKI
EVIHQQEKKFASILGIKEYQDIKDELVSKCLHAPVEFIATEIFYFADQVKAINSYSQQSKIISASRDSELALFKGIDPIV
NSDNTLPQFCHQQISIMIMADSIDLLESRVAYISQRLVKQGIIHVREDINLAQTFWSQLPGNFNYIRRVSYNIIDNVAAL
AALHHYPVGMQHSRWGRSVTIFRTECGTPYFMNFHDESAKGHTCIIGGAQSGRATVTNFLLSEASKYSPTILYLTNRNNA
QVFIKGIEGTWYNGDLPFNPLSYLESSDDLLELFKIITGSYFNNLSDSEIECLTELAIAVMDMPPESRSLIKIIKDFNFK
DYSAGASVKKRIKLFISDDKCSKIFNQSSTLELQDNNVTAINLADYTDYQFNQSYTPQVGEKPEIYHNKLQTLHCLRSGI
IFSLIKKFSLLDSSEPKILVVDNMPDLIVEQVFMNLIKQITEQLSENNGIILSVVQVNQNDQFYYSDFWKKWLKFNNTKI
ILPTNIRNLPIAEICNLSTSEAKKFNTISPNSRLFMVKQDNNYVIVELNLEGFPAIRKLLSAEEEDLNLFHKIMQKIGDD
APSAWLSDVYDQFQNSE

Sequences:

>Translated_817_residues
MGFIHKFLHSRQIDAKQFDSNAQNFIPITCHYNDETLLTKNGELIQILQIKGINSEYTNKDLLGIRAAIRNAIKNHINNN
NFAVWIHVVRRCDNIDDPTPYSQALPNFIHNVWSKKNYWQDKFVSTLYISIVHHGCNLNIRNLDSFLNSLSFKTLDNFYD
KFIASALKNLSMAINNIITELQRYNPCKLSIKIDNNKVYSDLLNFYKKIIQLNTGLIEVKDVDCAVQLATHKYSFGGDKI
EVIHQQEKKFASILGIKEYQDIKDELVSKCLHAPVEFIATEIFYFADQVKAINSYSQQSKIISASRDSELALFKGIDPIV
NSDNTLPQFCHQQISIMIMADSIDLLESRVAYISQRLVKQGIIHVREDINLAQTFWSQLPGNFNYIRRVSYNIIDNVAAL
AALHHYPVGMQHSRWGRSVTIFRTECGTPYFMNFHDESAKGHTCIIGGAQSGRATVTNFLLSEASKYSPTILYLTNRNNA
QVFIKGIEGTWYNGDLPFNPLSYLESSDDLLELFKIITGSYFNNLSDSEIECLTELAIAVMDMPPESRSLIKIIKDFNFK
DYSAGASVKKRIKLFISDDKCSKIFNQSSTLELQDNNVTAINLADYTDYQFNQSYTPQVGEKPEIYHNKLQTLHCLRSGI
IFSLIKKFSLLDSSEPKILVVDNMPDLIVEQVFMNLIKQITEQLSENNGIILSVVQVNQNDQFYYSDFWKKWLKFNNTKI
ILPTNIRNLPIAEICNLSTSEAKKFNTISPNSRLFMVKQDNNYVIVELNLEGFPAIRKLLSAEEEDLNLFHKIMQKIGDD
APSAWLSDVYDQFQNSE
>Mature_816_residues
GFIHKFLHSRQIDAKQFDSNAQNFIPITCHYNDETLLTKNGELIQILQIKGINSEYTNKDLLGIRAAIRNAIKNHINNNN
FAVWIHVVRRCDNIDDPTPYSQALPNFIHNVWSKKNYWQDKFVSTLYISIVHHGCNLNIRNLDSFLNSLSFKTLDNFYDK
FIASALKNLSMAINNIITELQRYNPCKLSIKIDNNKVYSDLLNFYKKIIQLNTGLIEVKDVDCAVQLATHKYSFGGDKIE
VIHQQEKKFASILGIKEYQDIKDELVSKCLHAPVEFIATEIFYFADQVKAINSYSQQSKIISASRDSELALFKGIDPIVN
SDNTLPQFCHQQISIMIMADSIDLLESRVAYISQRLVKQGIIHVREDINLAQTFWSQLPGNFNYIRRVSYNIIDNVAALA
ALHHYPVGMQHSRWGRSVTIFRTECGTPYFMNFHDESAKGHTCIIGGAQSGRATVTNFLLSEASKYSPTILYLTNRNNAQ
VFIKGIEGTWYNGDLPFNPLSYLESSDDLLELFKIITGSYFNNLSDSEIECLTELAIAVMDMPPESRSLIKIIKDFNFKD
YSAGASVKKRIKLFISDDKCSKIFNQSSTLELQDNNVTAINLADYTDYQFNQSYTPQVGEKPEIYHNKLQTLHCLRSGII
FSLIKKFSLLDSSEPKILVVDNMPDLIVEQVFMNLIKQITEQLSENNGIILSVVQVNQNDQFYYSDFWKKWLKFNNTKII
LPTNIRNLPIAEICNLSTSEAKKFNTISPNSRLFMVKQDNNYVIVELNLEGFPAIRKLLSAEEEDLNLFHKIMQKIGDDA
PSAWLSDVYDQFQNSE

Specific function: The type IV secretion system VirB/VirD4 is a major virulence determinant for subversion of human endothelial cell (HEC) function. VirB-dependent changes of HEC include massive cytoskeletal rearrangements, a proinflammatory activation by nuclear factor NF-

COG id: COG3451

COG function: function code U; Type IV secretory pathway, VirB4 components

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trbE/virB4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004346
- InterPro:   IPR018145 [H]

Pfam domain/function: PF03135 CagE_TrbE_VirB [H]

EC number: NA

Molecular weight: Translated: 93584; Mature: 93453

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFIHKFLHSRQIDAKQFDSNAQNFIPITCHYNDETLLTKNGELIQILQIKGINSEYTNK
CCHHHHHHHCCCCCHHHHCCCCCCEEEEEEEECCCEEEECCCCEEEEEEEECCCCCCCCC
DLLGIRAAIRNAIKNHINNNNFAVWIHVVRRCDNIDDPTPYSQALPNFIHNVWSKKNYWQ
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHH
DKFVSTLYISIVHHGCNLNIRNLDSFLNSLSFKTLDNFYDKFIASALKNLSMAINNIITE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQRYNPCKLSIKIDNNKVYSDLLNFYKKIIQLNTGLIEVKDVDCAVQLATHKYSFGGDKI
HHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEHEEECCCCCEE
EVIHQQEKKFASILGIKEYQDIKDELVSKCLHAPVEFIATEIFYFADQVKAINSYSQQSK
EEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IISASRDSELALFKGIDPIVNSDNTLPQFCHQQISIMIMADSIDLLESRVAYISQRLVKQ
HHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHH
GIIHVREDINLAQTFWSQLPGNFNYIRRVSYNIIDNVAALAALHHYPVGMQHSRWGRSVT
HHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
IFRTECGTPYFMNFHDESAKGHTCIIGGAQSGRATVTNFLLSEASKYSPTILYLTNRNNA
EEEECCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCC
QVFIKGIEGTWYNGDLPFNPLSYLESSDDLLELFKIITGSYFNNLSDSEIECLTELAIAV
EEEEEECCCEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MDMPPESRSLIKIIKDFNFKDYSAGASVKKRIKLFISDDKCSKIFNQSSTLELQDNNVTA
HCCCCCHHHHHHHHHHCCCCCCCCCCHHHEEEEEEECCCHHHHHHCCCCEEEEECCCEEE
INLADYTDYQFNQSYTPQVGEKPEIYHNKLQTLHCLRSGIIFSLIKKFSLLDSSEPKILV
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
VDNMPDLIVEQVFMNLIKQITEQLSENNGIILSVVQVNQNDQFYYSDFWKKWLKFNNTKI
ECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEE
ILPTNIRNLPIAEICNLSTSEAKKFNTISPNSRLFMVKQDNNYVIVELNLEGFPAIRKLL
EEECCCCCCCHHHHHCCCHHHHHHCCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHH
SAEEEDLNLFHKIMQKIGDDAPSAWLSDVYDQFQNSE
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
GFIHKFLHSRQIDAKQFDSNAQNFIPITCHYNDETLLTKNGELIQILQIKGINSEYTNK
CHHHHHHHCCCCCHHHHCCCCCCEEEEEEEECCCEEEECCCCEEEEEEEECCCCCCCCC
DLLGIRAAIRNAIKNHINNNNFAVWIHVVRRCDNIDDPTPYSQALPNFIHNVWSKKNYWQ
HHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHH
DKFVSTLYISIVHHGCNLNIRNLDSFLNSLSFKTLDNFYDKFIASALKNLSMAINNIITE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQRYNPCKLSIKIDNNKVYSDLLNFYKKIIQLNTGLIEVKDVDCAVQLATHKYSFGGDKI
HHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEHEEECCCCCEE
EVIHQQEKKFASILGIKEYQDIKDELVSKCLHAPVEFIATEIFYFADQVKAINSYSQQSK
EEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IISASRDSELALFKGIDPIVNSDNTLPQFCHQQISIMIMADSIDLLESRVAYISQRLVKQ
HHCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHEEEEEEECCHHHHHHHHHHHHHHHHHH
GIIHVREDINLAQTFWSQLPGNFNYIRRVSYNIIDNVAALAALHHYPVGMQHSRWGRSVT
HHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEE
IFRTECGTPYFMNFHDESAKGHTCIIGGAQSGRATVTNFLLSEASKYSPTILYLTNRNNA
EEEECCCCCEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCC
QVFIKGIEGTWYNGDLPFNPLSYLESSDDLLELFKIITGSYFNNLSDSEIECLTELAIAV
EEEEEECCCEEECCCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
MDMPPESRSLIKIIKDFNFKDYSAGASVKKRIKLFISDDKCSKIFNQSSTLELQDNNVTA
HCCCCCHHHHHHHHHHCCCCCCCCCCHHHEEEEEEECCCHHHHHHCCCCEEEEECCCEEE
INLADYTDYQFNQSYTPQVGEKPEIYHNKLQTLHCLRSGIIFSLIKKFSLLDSSEPKILV
EEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEE
VDNMPDLIVEQVFMNLIKQITEQLSENNGIILSVVQVNQNDQFYYSDFWKKWLKFNNTKI
ECCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCEE
ILPTNIRNLPIAEICNLSTSEAKKFNTISPNSRLFMVKQDNNYVIVELNLEGFPAIRKLL
EEECCCCCCCHHHHHCCCHHHHHHCCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHH
SAEEEDLNLFHKIMQKIGDDAPSAWLSDVYDQFQNSE
CCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10749166; 10882236; 7494028 [H]