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The map label for this gene is truA

Identifier: 148268659

GI number: 148268659

Start: 2390713

End: 2391516

Strand: Reverse

Name: truA

Synonym: SaurJH9_2246

Alternate gene names: 148268659

Gene position: 2391516-2390713 (Counterclockwise)

Preceding gene: 148268660

Following gene: 148268658

Centisome position: 82.28

GC content: 35.45

Gene sequence:

>804_bases
ATGCGTATATTAGTAGAAATTGCGTATCAAGGAAATAATTTTCTAGGCTTTCAAATTCAACAGAATGGACGTACGGTACA
GCAACAATTTGAAAAGCTATTACAACGCATGCATAAAAGACATGTGAGAATCCATCCTTCAAGTAGGACTGATAGAGGTG
TACATGCGATACAGCAGTACTTTCATTTCGACACAGAGCTGAATATACCTATGTCACAATGGCAATATGCAATGAACCGA
ACGTTACCAGATGATATTTATGTCAATAATGTAGTTACAGTCGATGACGATTTTCATTGTCGTTACGATTGTGTCGGTAA
ACGCTATCGATATAAAGTATATCAAGCACAACACCGCGATCCATTTCAAAGTGGTTTGAAAACATTTATTCCTGAAACGT
TGGATTTAGACAAAATGAACAGAGCGGCGCAACAATTCATAGGTACACATGATTTTACGGGGTTTTGTTCGCAAAAAACT
GAAGTAGAAAGCAAAGTTAGAACATTATACCAAAGTGAAATCGTAAAGACAGATGATGGCTTTGATTATATTGTCACTGG
ATCTGGATTTTTATATAACATGGTGCGTGTCTTAGTTGCATTTTTAATAGAAGTTGGAAAAGGGCGACATGAAATTTCTG
ATGTGCCGAAGCTACTTGAATCAAAGAATCGTAAGAATGTTCCTTTCACTGCACCGGCTGAAGGACTATATTTAGAGAAG
ATTTATTTAGATGAAAATGAGTTATTAAAAGACTTTGGCAACGATATAAAAATACATCGCAAAAAATCATTACAAAATGA
CTGA

Upstream 100 bases:

>100_bases
AGACAGCTTAAATGGCAATTGAGAGATACGATATCTTTAACCATGATTATCCCAATTGCAATTATTTTATTCGTATTAAA
ATATTCAGGAGTGTAAATTC

Downstream 100 bases:

>100_bases
TTGCCATTGACAAAAAGCTTGTGAAATTATAAGATTATTAACGGTATTGTTTTATATCCACCCCACGATAAGCCCCGGAA
ACTTATTGTGTTACAAGATA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRIHPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNR
TLPDDIYVNNVVTVDDDFHCRYDCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLDKMNRAAQQFIGTHDFTGFCSQKT
EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEISDVPKLLESKNRKNVPFTAPAEGLYLEK
IYLDENELLKDFGNDIKIHRKKSLQND

Sequences:

>Translated_267_residues
MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRIHPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNR
TLPDDIYVNNVVTVDDDFHCRYDCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLDKMNRAAQQFIGTHDFTGFCSQKT
EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEISDVPKLLESKNRKNVPFTAPAEGLYLEK
IYLDENELLKDFGNDIKIHRKKSLQND
>Mature_267_residues
MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRIHPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNR
TLPDDIYVNNVVTVDDDFHCRYDCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLDKMNRAAQQFIGTHDFTGFCSQKT
EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEISDVPKLLESKNRKNVPFTAPAEGLYLEK
IYLDENELLKDFGNDIKIHRKKSLQND

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=270, Percent_Identity=31.4814814814815, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI199559785, Length=267, Percent_Identity=26.2172284644195, Blast_Score=86, Evalue=3e-17,
Organism=Escherichia coli, GI1788657, Length=250, Percent_Identity=34.4, Blast_Score=149, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71985046, Length=163, Percent_Identity=28.8343558282209, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17565126, Length=298, Percent_Identity=25.1677852348993, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321112, Length=233, Percent_Identity=25.7510729613734, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI116007752, Length=307, Percent_Identity=29.3159609120521, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_STAA1 (A7X5B5)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442793.1
- ProteinModelPortal:   A7X5B5
- SMR:   A7X5B5
- STRING:   A7X5B5
- EnsemblBacteria:   EBSTAT00000003548
- GeneID:   5559997
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_2203
- eggNOG:   COG0101
- GeneTree:   EBGT00050000024223
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   SAUR418127:SAHV_2203-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 31352; Mature: 31352

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: NA

Important sites: ACT_SITE 51-51

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRIHPSSRTDRGVHAIQQY
CEEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHH
FHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRYDCVGKRYRYKVYQAQHRD
HHCCCCCCCCHHHHHHHHHCCCCCCEEECCEEEECCCCCEEHHHHCCHHHHHHHHCCCCC
PFQSGLKTFIPETLDLDKMNRAAQQFIGTHDFTGFCSQKTEVESKVRTLYQSEIVKTDDG
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHEECCCC
FDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEISDVPKLLESKNRKNVPFTAPAEGLYLEK
CEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
IYLDENELLKDFGNDIKIHRKKSLQND
EEECHHHHHHHCCCCEEEEEHHCCCCC
>Mature Secondary Structure
MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRIHPSSRTDRGVHAIQQY
CEEEEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHHCCEEECCCCCCHHHHHHHHHH
FHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRYDCVGKRYRYKVYQAQHRD
HHCCCCCCCCHHHHHHHHHCCCCCCEEECCEEEECCCCCEEHHHHCCHHHHHHHHCCCCC
PFQSGLKTFIPETLDLDKMNRAAQQFIGTHDFTGFCSQKTEVESKVRTLYQSEIVKTDDG
HHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHEECCCC
FDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEISDVPKLLESKNRKNVPFTAPAEGLYLEK
CEEEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEE
IYLDENELLKDFGNDIKIHRKKSLQND
EEECHHHHHHHCCCCEEEEEHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA