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The map label for this gene is nadE

Identifier: 148268386

GI number: 148268386

Start: 2102599

End: 2103420

Strand: Reverse

Name: nadE

Synonym: SaurJH9_1968

Alternate gene names: 148268386

Gene position: 2103420-2102599 (Counterclockwise)

Preceding gene: 148268387

Following gene: 148268385

Centisome position: 72.36

GC content: 34.06

Gene sequence:

>822_bases
ATGAGTAAATTACAAGACGTTATTGTACAAGAAATGAAAGTGAAAAAGCGTATCGATAGTGCTGAAGAAATTATGGAATT
AAAGCAATTTATAAAAAATTATGTACAATCACATTCATTTATAAAATCTTTAGTGTTAGGTATTTCAGGAGGACAAGATT
CTACATTAGTTGGAAAACTAGTACAAATGTCTGTTAACGAATTACGTGAAGAAGGCATTGATTGTACGTTTATTGCAGTT
AAATTACCTTATGGAGTTCAAAAAGATGCTGATGAAGTTGACCAAGCTTTGCGATTCATTGAACCAGATGAAATAGTAAC
AGTCAATATTAAGCCTGCAGTTGATCAAAGTGTGCAATCATTAAAAGAAGCTGGTATTGTTCTTACAGATTTCCAAAAAG
GAAATGAAAAAGCGCGTGAACGTATGAAAGTACAATTTTCAATTGCTTCAAACCGACAAGGTATTGTAGTAGGAACAGAT
CATTCAGCTGAAAATATAACTGGGTTTTATACGAAGTACGGTGATGGTGCTGCAGATATCGCACCTATATTTGGTTTGAA
TAAACGACAAGGTCGTCAATTATTAGCGTATCTTGGTGCGCCAAAGGAATTATATGAAAAAACGCCAACTGCTGATTTAG
AAGATGATAAACCACAGCTTCCAGATGAAGATGCATTAGGTGTAACTTATGAGGCGATTGATAATTATTTAGAAGGTAAG
CCAGTTACGCCAGAAGAACAAAAAGTAATTGAAAATCATTATATACGAAATGCACACAAACGTGAACTTGCATATACAAG
ATACACGTGGCCAAAATCCTAA

Upstream 100 bases:

>100_bases
CCACAAGAATATCCAGTCGATTTAAGCAAGGCATGTTGGGATAATAAACATAAACGTATTTTTGAAGTTGCGGAACACGT
TAAGGAGATGGAAGAAGATA

Downstream 100 bases:

>100_bases
TTTAATTTTTTCTTCTAACGTGTGACTTAAATTAAATATGAGTTAGAATTAATAACATTAAACCACATTCAGCTAGACTA
CTTCAGTGTATAAATTGAAA

Product: NAD synthetase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSLVLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAV
KLPYGVQKDADEVDQALRFIEPDEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKPQLPDEDALGVTYEAIDNYLEGK
PVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS

Sequences:

>Translated_273_residues
MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSLVLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAV
KLPYGVQKDADEVDQALRFIEPDEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKPQLPDEDALGVTYEAIDNYLEGK
PVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS
>Mature_272_residues
SKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSLVLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVK
LPYGVQKDADEVDQALRFIEPDEIVTVNIKPAVDQSVQSLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTDH
SAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKPQLPDEDALGVTYEAIDNYLEGKP
VTPEEQKVIENHYIRNAHKRELAYTRYTWPKS

Specific function: This NAD Synthase Uses Nh(3) In Preference To Glutamine. [C]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD synthetase family

Homologues:

Organism=Escherichia coli, GI1788036, Length=273, Percent_Identity=58.6080586080586, Blast_Score=318, Evalue=2e-88,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NADE_STAA1 (A7X442)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442487.1
- ProteinModelPortal:   A7X442
- SMR:   A7X442
- STRING:   A7X442
- EnsemblBacteria:   EBSTAT00000004785
- GeneID:   5560487
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1897
- eggNOG:   COG0171
- GeneTree:   EBGT00050000025036
- HOGENOM:   HBG351567
- OMA:   KVAQATI
- ProtClustDB:   PRK00768
- BioCyc:   SAUR418127:SAHV_1897-MONOMER
- HAMAP:   MF_00193
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR022926
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- TIGRFAMs:   TIGR00552

Pfam domain/function: PF02540 NAD_synthase

EC number: =6.3.1.5

Molecular weight: Translated: 30683; Mature: 30552

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: ACT_SITE 49-49

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSLVLGISGGQDSTLVGKL
CCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VQMSVNELREEGIDCTFIAVKLPYGVQKDADEVDQALRFIEPDEIVTVNIKPAVDQSVQS
HHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHH
LKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTDHSAENITGFYTKYGDGAADI
HHHCCCEEEECCCCCHHHHHHHEEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCHHH
APIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKPQLPDEDALGVTYEAIDNYLEGK
HHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCC
PVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS
CCCCHHHHHHHHHHHHCHHHHHHHHEECCCCCC
>Mature Secondary Structure 
SKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSLVLGISGGQDSTLVGKL
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VQMSVNELREEGIDCTFIAVKLPYGVQKDADEVDQALRFIEPDEIVTVNIKPAVDQSVQS
HHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECHHHHHHHHH
LKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTDHSAENITGFYTKYGDGAADI
HHHCCCEEEECCCCCHHHHHHHEEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCHHH
APIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKPQLPDEDALGVTYEAIDNYLEGK
HHHHCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHCCC
PVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS
CCCCHHHHHHHHHHHHCHHHHHHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA