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The map label for this gene is purB

Identifier: 148268381

GI number: 148268381

Start: 2097602

End: 2098897

Strand: Reverse

Name: purB

Synonym: SaurJH9_1963

Alternate gene names: 148268381

Gene position: 2098897-2097602 (Counterclockwise)

Preceding gene: 148268384

Following gene: 148268380

Centisome position: 72.21

GC content: 35.49

Gene sequence:

>1296_bases
ATGATTGAACGCTATTCTAGAGAAGAAATGTCTAATATTTGGACTGATCAAAATCGCTATGAAGCATGGTTAGAAGTGGA
AATTTTAGCATGTGAAGCATGGAGTGAATTAGGACATATACCGAAAGCTGACGTACAAAAAATTCGTCAAAACGCAAAGG
TAAATGTCGAACGTGCACAAGAAATTGAACAAGAAACGCGCCATGATGTTGTAGCCTTTACTAGACAAGTTTCTGAAACG
CTAGGTGAAGAACGTAAGTGGGTACATTATGGTTTAACTTCTACTGATGTTGTAGATACAGCTTTAAGTTTCGTTATTAA
ACAAGCAAATGACATTATTGAAAAAGATTTAGAAAGATTTATCGACGTTTTAGCTGAAAAAGCAAAAAATTATAAATATA
CATTGATGATGGGACGTACACATGGAGTGCATGCAGAACCAACTACATTTGGTGTCAAAATGGCATTGTGGTACACGGAA
ATGCAACGTAACTTACAACGTTTCAAACAAGTAAGAGAAGAAATTGAAGTAGGGAAAATGAGTGGTGCAGTAGGTACTTT
TGCTAACATTCCTCCTGAAATAGAAAGTTATGTGTGTAAACATTTAGGTATTGGCACAGCGCCTGTATCAACGCAAACAT
TGCAACGCGATCGACATGCATACTATATTGCGACATTAGCATTAATCGCGACGTCACTAGAAAAATTTGCCGTTGAAATT
CGTAACCTTCAAAAAACCGAAACAAGAGAAGTTGAAGAAGCATTTGCAAAAGGCCAAAAAGGTTCATCTGCAATGCCTCA
TAAACGAAATCCAATTGGTTCTGAAAATATCACAGGTATTTCAAGAGTCATTCGTGGTTATATTACTACAGCTTATGAGA
ATGTGCCATTATGGCATGAAAGAGATATTTCTCATTCTTCTGCTGAGCGTATTATGTTACCAGATGTTACAATAGCACTA
GATTATGCATTGAATCGTTTCACAAATATTGTGGACCGTTTAACTGTATTTGAAGATAATATGCGTAATAACATAGATAA
AACATTTGGTCTGATTTTCTCACAACGTGTCTTATTAGCATTAATTAATAAAGGTATGGTTCGTGAAGAAGCATACGACA
AAGTACAACCAAAAGCGATGATATCTTGGGAAACGAAAACACCTTTCCGTGAATTAATCGAACAAGATGAGTCTATTACA
AGTGTTTTAACAAAAGAAGAATTAGATGAATGTTTCGATCCTAAACATCATTTGAATCAAGTTGACACTATTTTCGAACG
CGCTGGATTAGCATAA

Upstream 100 bases:

>100_bases
AACTGTGAAGAGTAGAGTTAAAAACGAACTTTTTGTTTATTTTTATTAAAAATGTTCTGTTTTTGAAAATTCGAGTATAC
ATACGATAGGAGTTAAAAAA

Downstream 100 bases:

>100_bases
AATTAAATAAAGTAACGATTCAGATTTAATAAAATATAAATCGCTACACTTTAATAGATTAATGCGTTAAAATGACATAA
AGAATAAAATTATAGGGGTT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKADVQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSET
LGEERKWVHYGLTSTDVVDTALSFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDRHAYYIATLALIATSLEKFAVEI
RNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSENITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIAL
DYALNRFTNIVDRLTVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFRELIEQDESIT
SVLTKEELDECFDPKHHLNQVDTIFERAGLA

Sequences:

>Translated_431_residues
MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKADVQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSET
LGEERKWVHYGLTSTDVVDTALSFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDRHAYYIATLALIATSLEKFAVEI
RNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSENITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIAL
DYALNRFTNIVDRLTVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFRELIEQDESIT
SVLTKEELDECFDPKHHLNQVDTIFERAGLA
>Mature_431_residues
MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKADVQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSET
LGEERKWVHYGLTSTDVVDTALSFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDRHAYYIATLALIATSLEKFAVEI
RNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSENITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIAL
DYALNRFTNIVDRLTVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFRELIEQDESIT
SVLTKEELDECFDPKHHLNQVDTIFERAGLA

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily

Homologues:

Organism=Homo sapiens, GI4557269, Length=441, Percent_Identity=26.0770975056689, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI183227688, Length=399, Percent_Identity=27.5689223057644, Blast_Score=155, Evalue=8e-38,
Organism=Escherichia coli, GI1787376, Length=317, Percent_Identity=28.7066246056782, Blast_Score=93, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17508577, Length=396, Percent_Identity=26.010101010101, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI32564234, Length=355, Percent_Identity=26.7605633802817, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323391, Length=434, Percent_Identity=28.8018433179723, Blast_Score=163, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24647570, Length=437, Percent_Identity=26.3157894736842, Blast_Score=137, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR8_STAA3 (Q2FFI7)

Other databases:

- EMBL:   CP000255
- RefSeq:   YP_494541.1
- ProteinModelPortal:   Q2FFI7
- SMR:   Q2FFI7
- STRING:   Q2FFI7
- EnsemblBacteria:   EBSTAT00000037565
- GeneID:   3914957
- GenomeReviews:   CP000255_GR
- KEGG:   saa:SAUSA300_1889
- eggNOG:   COG0015
- GeneTree:   EBGT00050000024473
- HOGENOM:   HBG499008
- OMA:   KVWEQGK
- ProtClustDB:   PRK08937
- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769
- PRINTS:   PR00145
- PRINTS:   PR00149
- TIGRFAMs:   TIGR00928

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1; SSF48557 L-Aspartase-like

EC number: =4.3.2.2

Molecular weight: Translated: 49604; Mature: 49604

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: ACT_SITE 68-68 ACT_SITE 141-141

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKADVQKIRQNAKVNVERAQ
CCCCCCHHHHHHHCCCCCHHHHEEEEEEEEHHHHHHHCCCCHHHHHHHHHCCCCCHHHHH
EIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTALSFVIKQANDIIEKDLERF
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTEMQRNLQRFKQVREEIEVGKM
HHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDRHAYYIATLALIATSLEKFAVEI
CCCHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSENITGISRVIRGYITTAYENVPLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
RDISHSSAERIMLPDVTIALDYALNRFTNIVDRLTVFEDNMRNNIDKTFGLIFSQRVLLA
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LINKGMVREEAYDKVQPKAMISWETKTPFRELIEQDESITSVLTKEELDECFDPKHHLNQ
HHHCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHH
VDTIFERAGLA
HHHHHHHCCCC
>Mature Secondary Structure
MIERYSREEMSNIWTDQNRYEAWLEVEILACEAWSELGHIPKADVQKIRQNAKVNVERAQ
CCCCCCHHHHHHHCCCCCHHHHEEEEEEEEHHHHHHHCCCCHHHHHHHHHCCCCCHHHHH
EIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTALSFVIKQANDIIEKDLERF
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTEMQRNLQRFKQVREEIEVGKM
HHHHHHHHCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDRHAYYIATLALIATSLEKFAVEI
CCCHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSENITGISRVIRGYITTAYENVPLWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
RDISHSSAERIMLPDVTIALDYALNRFTNIVDRLTVFEDNMRNNIDKTFGLIFSQRVLLA
CCCCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LINKGMVREEAYDKVQPKAMISWETKTPFRELIEQDESITSVLTKEELDECFDPKHHLNQ
HHHCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHH
VDTIFERAGLA
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA