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The map label for this gene is ligA

Identifier: 148268377

GI number: 148268377

Start: 2092131

End: 2094134

Strand: Reverse

Name: ligA

Synonym: SaurJH9_1959

Alternate gene names: 148268377

Gene position: 2094134-2092131 (Counterclockwise)

Preceding gene: 148268378

Following gene: 148268376

Centisome position: 72.05

GC content: 33.98

Gene sequence:

>2004_bases
ATGGCTGATTTATCGTCTCGTGTGAACGAGTTACATGATTTATTAAATCAATACAGTTATGAATACTATGTAGAGGATAA
TCCATCTGTACCAGATAGTGAATATGACAAATTACTTCATGAACTGATTAAAATAGAAGAGGAGCATCCTGAGTATAAGA
CTGTAGATTCTCCAACAGTTAGAGTTGGCGGTGAAGCCCAAGCCTCTTTCAAAAAAGTCAACCATGACACGCCAATGTTA
AGTTTAGGGAATGCATTTAATGAGGATGATTTGAGAAAATTCGACCAACGCATACGTGAACAAATTGGCAACGTTGAATA
TATGTGCGAATTAAAAATTGATGGCTTAGCAGTATCATTGAAATATGTTGATGGATACTTCGTTCAAGGTTTAACACGTG
GTGATGGAACAACAGGTGAAGATATTACCGAAAATTTAAAAACAATTCATGCGATACCTTTGAAAATGAAAGAACCATTA
AATGTAGAAGTTCGTGGTGAAGCATATATGCCGAGACGTTCATTTTTACGATTAAATGAAGAAAAAGAAAAAAATGATGA
GCAGTTATTTGCAAATCCAAGAAACGCTGCTGCGGGATCATTAAGACAGTTAGATTCTAAATTAACGGCAAAACGAAAGC
TAAGCGTATTTATATATAGTGTCAATGATTTCACTGATTTCAATGCGCGTTCGCAAAGTGAAGCATTAGATGAGTTAGAT
AAATTAGGTTTTACAACGAATAAAAATAGAGCGCGTGTAAATAATATCGATGGTGTTTTAGAGTATATTGAAAAATGGAC
AAGCCAAAGAGAGTCATTACCTTATGATATTGATGGGATTGTTATTAAGGTTAATGATTTAGATCAACAGGATGAGATGG
GATTCACACAAAAATCTCCTAGATGGGCCATTGCTTATAAATTTCCAGCTGAGGAAGTAGTAACTAAATTATTAGATATT
GAATTAAGTATTGGACGAACAGGTGTAGTCACACCTACTGCTATTTTAGAACCAGTAAAAGTAGCTGGTACAACTGTATC
AAGAGCATCTTTGCACAATGAGGATTTAATTCATGACAGAGATATTCGAATTGGTGATAGTGTTGTAGTGAAAAAAGCAG
GTGACATCATACCTGAAGTTGTACGTAGTATTCCAGAACGTAGACCTGAGGATGCTGTCACATATCATATGCCAACCCAT
TGTCCAAGTTGTGGACATGAATTAGTACGTATTGAAGGCGAAGTAGCACTTCGTTGCATTAATCCAAAATGCCAAGCACA
ACTTGTTGAAGGATTAATTCACTTTGTATCAAGACAAGCCATGAATATTGATGGTTTAGGCACTAAAATTATTCAACAGC
TTTATCAAAGCGAATTAATTAAAGATGTTGCTGATATTTTCTATTTAACAGAAGAAGATTTATTACCTTTAGATAGAATG
GGGCAGAAAAAAGTTGATAATTTATTAGCTGCCATTCAACAAGCTAAGGACAACTCTTTAGAAAATTTATTATTTGGTCT
AGGTATTAGGCATTTAGGTGTTAAAGCGAGCCAAGTGTTAGCAGAAAAATATGAAACGATAGATCGATTACTAACGGTAA
CTGAAGCGGAATTAGTAGAAATTCATGATATAGGTGATAAAGTAGCACAATCTGTAGTTACTTATTTAGAAAATGAAGAT
ATTCGTGCTTTAATTCAAAAATTAAAAGATAAACATGTTAATATGATTTATAAAGGTATCAAAACATCAGATATTGAAGG
ACATCCTGAATTTAGTGGTAAAACGATAGTACTGACTGGTAAGCTACATCAAATGACACGCAATGAAGCATCTAAATGGC
TTGCATCACAAGGTGCTAAAGTTACAAGTAGCGTTACTAAAAATACAGATGTCGTTATTGCTGGTGAAGATGCAGGTTCA
AAATTAACAAAAGCACAAAGTTTAGGTATTGAAATTTGGACAGAGCAACAATTTGTAGATAAGCAAAATGAATTAAATAG
TTAG

Upstream 100 bases:

>100_bases
GAAAAATGGCTCAATCGAACTAGATATTATCTTTAAATCACAAGGGCCAAAACGTTTGTTAGCGCAATTTGCACCAATTG
AAAAAAAGGAGGATTAAGGG

Downstream 100 bases:

>100_bases
AGGGGTATGTCGATGAAGCGTACATTAGTATTATTGATTACAGCTATCTTTATACTCGCTGCTTGTGGTAACCATAAGGA
TGACCAGGCTGGAAAAGATA

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 667; Mature: 666

Protein sequence:

>667_residues
MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIKIEEEHPEYKTVDSPTVRVGGEAQASFKKVNHDTPML
SLGNAFNEDDLRKFDQRIREQIGNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFIYSVNDFTDFNARSQSEALDELD
KLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYDIDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDI
ELSIGRTGVVTPTAILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDAVTYHMPTH
CPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKIIQQLYQSELIKDVADIFYLTEEDLLPLDRM
GQKKVDNLLAAIQQAKDNSLENLLFGLGIRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENED
IRALIQKLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSSVTKNTDVVIAGEDAGS
KLTKAQSLGIEIWTEQQFVDKQNELNS

Sequences:

>Translated_667_residues
MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIKIEEEHPEYKTVDSPTVRVGGEAQASFKKVNHDTPML
SLGNAFNEDDLRKFDQRIREQIGNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFIYSVNDFTDFNARSQSEALDELD
KLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYDIDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDI
ELSIGRTGVVTPTAILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDAVTYHMPTH
CPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKIIQQLYQSELIKDVADIFYLTEEDLLPLDRM
GQKKVDNLLAAIQQAKDNSLENLLFGLGIRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENED
IRALIQKLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSSVTKNTDVVIAGEDAGS
KLTKAQSLGIEIWTEQQFVDKQNELNS
>Mature_666_residues
ADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIKIEEEHPEYKTVDSPTVRVGGEAQASFKKVNHDTPMLS
LGNAFNEDDLRKFDQRIREQIGNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPLN
VEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFIYSVNDFTDFNARSQSEALDELDK
LGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYDIDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIE
LSIGRTGVVTPTAILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDAVTYHMPTHC
PSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKIIQQLYQSELIKDVADIFYLTEEDLLPLDRMG
QKKVDNLLAAIQQAKDNSLENLLFGLGIRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDI
RALIQKLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSSVTKNTDVVIAGEDAGSK
LTKAQSLGIEIWTEQQFVDKQNELNS

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=667, Percent_Identity=44.6776611694153, Blast_Score=577, Evalue=1e-166,
Organism=Escherichia coli, GI87082305, Length=574, Percent_Identity=23.1707317073171, Blast_Score=135, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_STAA1 (A7X432)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001442479.1
- ProteinModelPortal:   A7X432
- STRING:   A7X432
- EnsemblBacteria:   EBSTAT00000004316
- GeneID:   5560508
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1889
- eggNOG:   COG0272
- GeneTree:   EBGT00050000024212
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   CLSK885639
- BioCyc:   SAUR418127:SAHV_1889-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 75096; Mature: 74965

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 112-112 BINDING 110-110 BINDING 133-133 BINDING 167-167 BINDING 283-283 BINDING 307-307

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIKIEEEHPEYKTVDSPTV
CCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE
RVGGEAQASFKKVNHDTPMLSLGNAFNEDDLRKFDQRIREQIGNVEYMCELKIDGLAVSL
EECCCHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCEEEEE
KYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPLNVEVRGEAYMPRRSFLRLNE
EECCCCEEECEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHCCC
EKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFIYSVNDFTDFNARSQSEALDELD
HHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHH
KLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYDIDGIVIKVNDLDQQDEMGFTQKSP
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHCCCCCCCC
RWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTAILEPVKVAGTTVSRASLHNEDLIHDR
CEEEEEECCHHHHHHHHHHHEEECCCCCCCCHHHHHCCHHHCCCCHHHHHCCCCCCCCCC
DIRIGDSVVVKKAGDIIPEVVRSIPERRPEDAVTYHMPTHCPSCGHELVRIEGEVALRCI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHEEEECEEEEEEC
NPKCQAQLVEGLIHFVSRQAMNIDGLGTKIIQQLYQSELIKDVADIFYLTEEDLLPLDRM
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHC
GQKKVDNLLAAIQQAKDNSLENLLFGLGIRHLGVKASQVLAEKYETIDRLLTVTEAELVE
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IHDIGDKVAQSVVTYLENEDIRALIQKLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEC
KLHQMTRNEASKWLASQGAKVTSSVTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVD
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEECCCCCCHHHHHHHCCEEEECCHHHHC
KQNELNS
CHHCCCC
>Mature Secondary Structure 
ADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIKIEEEHPEYKTVDSPTV
CCHHHHHHHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCE
RVGGEAQASFKKVNHDTPMLSLGNAFNEDDLRKFDQRIREQIGNVEYMCELKIDGLAVSL
EECCCHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHHHHCCEEEEEEEEECCEEEEE
KYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPLNVEVRGEAYMPRRSFLRLNE
EECCCCEEECEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCHHHHHHCCC
EKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFIYSVNDFTDFNARSQSEALDELD
HHCCCCHHHHCCCCCHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCHHHHHHHHH
KLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYDIDGIVIKVNDLDQQDEMGFTQKSP
HCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCHHHCCCCCCCC
RWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTAILEPVKVAGTTVSRASLHNEDLIHDR
CEEEEEECCHHHHHHHHHHHEEECCCCCCCCHHHHHCCHHHCCCCHHHHHCCCCCCCCCC
DIRIGDSVVVKKAGDIIPEVVRSIPERRPEDAVTYHMPTHCPSCGHELVRIEGEVALRCI
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCHHEEEECEEEEEEC
NPKCQAQLVEGLIHFVSRQAMNIDGLGTKIIQQLYQSELIKDVADIFYLTEEDLLPLDRM
CHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHC
GQKKVDNLLAAIQQAKDNSLENLLFGLGIRHLGVKASQVLAEKYETIDRLLTVTEAELVE
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IHDIGDKVAQSVVTYLENEDIRALIQKLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTG
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEC
KLHQMTRNEASKWLASQGAKVTSSVTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVD
HHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEECCCCCCHHHHHHHCCEEEECCHHHHC
KQNELNS
CHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA