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The map label for this gene is vraR

Identifier: 148268356

GI number: 148268356

Start: 2070437

End: 2071066

Strand: Reverse

Name: vraR

Synonym: SaurJH9_1938

Alternate gene names: 148268356

Gene position: 2071066-2070437 (Counterclockwise)

Preceding gene: 148268357

Following gene: 148268352

Centisome position: 71.25

GC content: 33.97

Gene sequence:

>630_bases
ATGACGATTAAAGTATTGTTTGTGGATGATCATGAAATGGTACGTATAGGAATTTCAAGTTATCTATCAACGCAAAGTGA
TATTGAAGTAGTTGGTGAAGGCGCTTCTGGTAAAGAAGCAATTGCCAAAGCCCATGAGTTGAAGCCAGATTTAATTTTAA
TGGATTTACTTATGGAAGACATGGATGGTGTAGAAGCGACGACTCAGATTAAAAAAGATTTACCGCAAATTAAAGTATTA
ATGTTAACTAGTTTTATTGAAGATAAAGAGGTATATCGTGCATTAGATGCAGGTGTCGATAGTTACATTTTAAAAACAAC
AAGTGCAAAAGATATCGCCGATGCAGTTCGTAAAACTTCTAGAGGAGAATCTGTTTTTGAACCGGAAGTTTTAGTGAAAA
TGCGTAACCGTATGAAAAAGCGCGCAGAGTTATATGAAATGCTTACAGAACGAGAAATGGAAATATTATTATTGATTGCG
AAAGGTTACTCAAATCAAGAAATTGCTAGTGCATCGCATATTACTATTAAAACGGTTAAGACACATGTGAGTAACATTTT
AAGTAAGTTAGAAGTGCAAGATAGAACACAAGCTGTCATCTATGCATTCCAACATAATTTAATTCAATAG

Upstream 100 bases:

>100_bases
GAAAGAGCTTTGGAAATTGGTGCAACGTTCCATATTGTATCATTGCCAGATTCAGGTACACGTATCGAGGTGAAAGCACC
TTTAAATAAGGAGGATTCGT

Downstream 100 bases:

>100_bases
TTCGTATCGAATTAAGAAAAGTTACTTACGCCAATCACAATATAACATCAAATAGACACCAAAAAACAGAGGTCTTCGCA
ATCATTAGCGAAAACCTCTG

Product: two component LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 209; Mature: 208

Protein sequence:

>209_residues
MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKAHELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVL
MLTSFIEDKEVYRALDAGVDSYILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ

Sequences:

>Translated_209_residues
MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKAHELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVL
MLTSFIEDKEVYRALDAGVDSYILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ
>Mature_208_residues
TIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKAHELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLM
LTSFIEDKEVYRALDAGVDSYILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIAK
GYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ

Specific function: Member of the two-component regulatory system vraS/vraR involved in the control of the cell wall peptidoglycan biosynthesis. VraR is overexpressed in strain Mu3, which leads to vancomycin resistance

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain

Homologues:

Organism=Escherichia coli, GI1788521, Length=205, Percent_Identity=35.609756097561, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=30.4761904761905, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787473, Length=209, Percent_Identity=33.9712918660287, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1790102, Length=192, Percent_Identity=29.6875, Blast_Score=91, Evalue=4e-20,
Organism=Escherichia coli, GI1786747, Length=205, Percent_Identity=30.2439024390244, Blast_Score=87, Evalue=6e-19,
Organism=Escherichia coli, GI1788712, Length=193, Percent_Identity=23.8341968911917, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788546, Length=203, Percent_Identity=25.1231527093596, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87082052, Length=104, Percent_Identity=33.6538461538462, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1789937, Length=171, Percent_Identity=28.0701754385965, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): VRAR_STAA1 (P0C0Z1)

Other databases:

- EMBL:   AB035448
- EMBL:   AP009324
- RefSeq:   YP_001442459.1
- ProteinModelPortal:   P0C0Z1
- SMR:   P0C0Z1
- STRING:   P0C0Z1
- EnsemblBacteria:   EBSTAT00000004870
- GeneID:   5560535
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1869
- eggNOG:   COG2197
- GeneTree:   EBGT00050000024054
- HOGENOM:   HBG753323
- ProtClustDB:   CLSK885625
- BioCyc:   SAUR418127:SAHV_1869-MONOMER
- GO:   GO:0005737
- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00038
- SMART:   SM00421
- SMART:   SM00448

Pfam domain/function: PF00196 GerE; PF00072 Response_reg; SSF46894 Bipartite_resp_reg_C-effector; SSF52172 CheY_like

EC number: NA

Molecular weight: Translated: 23560; Mature: 23428

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00622 HTH_LUXR_1; PS50043 HTH_LUXR_2; PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKAHELKPDLILMDLLMED
CEEEEEEECCCHHHHHHHHHHHCCCCCHHEEECCCCCHHHHHHHHHCCCHHHHHHHHHHH
MDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSYILKTTSAKDIADAVRKTS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECCCHHHHHHHHHHHC
RGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIAKGYSNQEIASASHITIKTVK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHH
THVSNILSKLEVQDRTQAVIYAFQHNLIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKAHELKPDLILMDLLMED
EEEEEEECCCHHHHHHHHHHHCCCCCHHEEECCCCCHHHHHHHHHCCCHHHHHHHHHHH
MDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSYILKTTSAKDIADAVRKTS
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHEECCCHHHHHHHHHHHC
RGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIAKGYSNQEIASASHITIKTVK
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHH
THVSNILSKLEVQDRTQAVIYAFQHNLIQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580