Click here to switch to the map view.

The map label for this gene is thiI [H]

Identifier: 148268194

GI number: 148268194

Start: 1880402

End: 1881625

Strand: Reverse

Name: thiI [H]

Synonym: SaurJH9_1771

Alternate gene names: 148268194

Gene position: 1881625-1880402 (Counterclockwise)

Preceding gene: 148268195

Following gene: 148268193

Centisome position: 64.73

GC content: 33.01

Gene sequence:

>1224_bases
ATGAAGTATGATCACTTGCTTGTTAGATACGGGGAGTTAACATTAAAGGGTTCAAATAGAAAAAAATTTGTAAATCAATT
AAGAAATAATGTAAATAAGTCATTAAAAGGACTTGATGGGTTTGTCGTTAAAGGCAAACGAGATCGTATGTATATTGAAC
TTGAAGACCATGCAGATATAAATGAAATAACATATCGATTATCAAAAATTTTCGGTATTAAATCTATTAGTCCAGTATTA
AAAGTAGAAAAAACAATAGAGGCAATGAGTGCAGCGGCAATTAAATTTGCGCAGCAATTTGAAGAAAACAGCACATTTAA
AATTGATGTGAAGCGTGCCGATAAAAATTTCCCAATGGATACGTATGAATTACAACGTGAATTGGGTGGTACCGTATTGA
AACAAATTGAAAATGTTTCTGTTAATGTCAAACGTCCAGATCATGAAATTCGAGTGGAAGTTAGATTAGATGCAATTTAT
ATGTATGAAGAAGTTGTTCCGGGTTCAGGCGGATTACCAGTTGGTACTGGTGGTAAGACGTTACTAATGCTTTCAGGCGG
TATAGACTCACCAGTTGCTGGAATGGAAGTGATGAGACGTGGCGTAACAATTGAAGCGATTCATTTCCATAGTCCACCAT
TTACAAGTGATCAAGCAAAAGAAAAAGTTATTGAATTGACACGTATTTTAGCTGAACGTGTTGGACCAATTAAATTGCAT
ATTGTACCATTTACAGAATTGCAAAAACGGGTAAATAAAGTTGTACATCCAAGATATACAATGACTTCAACGAGACGTAT
GATGATGCGTGTTGCTGATAAATTAGTACATCAAATAGGGGCTTTAGCTATTGTAAATGGTGAAAACCTAGGGCAGGTAG
CCAGTCAAACACTTCATAGCATGTATGCAATTAATAATGTAACTTCTACTCCTGTATTACGTCCTTTATTAACTTACGAT
AAAGAAGAAATTATTATTAAATCGAAAGAAATTGGTACATTTGAAACATCTATTCAACCATTTGAAGATTGTTGTACAAT
TTTCACCCCTAAAAATCCAGTAACCGAACCAAACTTTGAAAAGGTAGTCCAATATGAAAGTGTCTTTGATTTTGAAGAGA
TGATTAATCGTGCTGTTGAAAATATTGAAACACTTGAAATAACTAGTGATTATAAAACTATTAAAGAACAGCAAACAAAC
CAATTAATAAATGACTTTTTATAA

Upstream 100 bases:

>100_bases
TAAGATTATCATTTGGGGCTACTACAACTAAAGAAGATATAGCAAGGTTTAAAGAAATATTTATCATCATTTATGAGGAA
ATTAAGGAGTTGCTAAAATA

Downstream 100 bases:

>100_bases
ATAAAATCCTAGAGTAAATTTAAACATAAGGGGATGTTAAACTATGGATTTGAACTTAACGATGATTATAATCATAATTT
TATTTGGTTTTATCGCGGCG

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase [H]

Number of amino acids: Translated: 407; Mature: 407

Protein sequence:

>407_residues
MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADINEITYRLSKIFGIKSISPVL
KVEKTIEAMSAAAIKFAQQFEENSTFKIDVKRADKNFPMDTYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIY
MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLH
IVPFTELQKRVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYD
KEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFEKVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTN
QLINDFL

Sequences:

>Translated_407_residues
MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADINEITYRLSKIFGIKSISPVL
KVEKTIEAMSAAAIKFAQQFEENSTFKIDVKRADKNFPMDTYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIY
MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLH
IVPFTELQKRVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYD
KEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFEKVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTN
QLINDFL
>Mature_407_residues
MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADINEITYRLSKIFGIKSISPVL
KVEKTIEAMSAAAIKFAQQFEENSTFKIDVKRADKNFPMDTYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIY
MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLH
IVPFTELQKRVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYD
KEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFEKVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTN
QLINDFL

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1786625, Length=386, Percent_Identity=30.3108808290155, Blast_Score=143, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114 [H]

Pfam domain/function: PF02568 ThiI; PF02926 THUMP [H]

EC number: =2.8.1.4 [H]

Molecular weight: Translated: 46257; Mature: 46257

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: PS51165 THUMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADI
CCCCCEEEEECEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCH
NEITYRLSKIFGIKSISPVLKVEKTIEAMSAAAIKFAQQFEENSTFKIDVKRADKNFPMD
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCH
TYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIYMYEEVVPGSGGLPVGTGGKT
HHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEEEEEEEEEHHHCCCCCCCCCCCCCCE
LLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLH
EEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
IVPFTELQKRVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHS
EECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHH
MYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFE
HHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHEEECCCCCCCCCCHH
KVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTNQLINDFL
HHHHHHHHCCHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVKGKRDRMYIELEDHADI
CCCCCEEEEECEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCH
NEITYRLSKIFGIKSISPVLKVEKTIEAMSAAAIKFAQQFEENSTFKIDVKRADKNFPMD
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCH
TYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIYMYEEVVPGSGGLPVGTGGKT
HHHHHHHHHHHHHHHHHCCEEEEECCCCEEEEEEEEEEEEEEHHHCCCCCCCCCCCCCCE
LLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQAKEKVIELTRILAERVGPIKLH
EEEEECCCCCCHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCEEEE
IVPFTELQKRVNKVVHPRYTMTSTRRMMMRVADKLVHQIGALAIVNGENLGQVASQTLHS
EECHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHHHH
MYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTFETSIQPFEDCCTIFTPKNPVTEPNFE
HHHHCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHEEECCCCCCCCCCHH
KVVQYESVFDFEEMINRAVENIETLEITSDYKTIKEQQTNQLINDFL
HHHHHHHHCCHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA