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The map label for this gene is folC [H]

Identifier: 148268143

GI number: 148268143

Start: 1817415

End: 1818686

Strand: Reverse

Name: folC [H]

Synonym: SaurJH9_1720

Alternate gene names: 148268143

Gene position: 1818686-1817415 (Counterclockwise)

Preceding gene: 148268144

Following gene: 148268142

Centisome position: 62.57

GC content: 32.78

Gene sequence:

>1272_bases
ATGAATTACCTAGAGAGCTTGTATTGGATACACGAAAGAACTAAATTTGGCATCAAACCAGGTGTTAAACGTATGGAATG
GATGCTAGCACAATTTAATAATCCTCAAAATAACATTAAGGGTATTCATGTAGGTGGCACAAATGGTAAAGGCTCTACAG
TTGCTTACCTTAGAACAGCTTTAGTTGAAAATGGTTATGAAGTAGGTACATTTACGTCGCCGTTTATTGAAACATTTAAT
GAACGAATTAGTCTAAATGGTGTGCCAATATCAAATGACGCTATTGTAGAATTAGTATCACGTATTAAACCAGTAAGTGA
AATGATGGAACGTGAAACAGATTTAGGTGTTGCAACTGAATTCGAAATAATCACAGCGATGATGTTTTTATATTTTGGTG
AAATACATCCTGTTGATTTTGTCATTGTTGAGGCTGGATTGGGTATAAAGAACGATTCGACAAATGTCTTTACACCGGTT
TTATCAATCTTAACTAGTATCGGTCTAGACCATACAGATATTTTAGGTGGTACTTATCTAGATATTGCTAGGGATAAAGG
CGCGATTATAAAGCCTAACGTTCCAGTGATATATGCTGTTAAAAATGAAGATGCATTAAAATATGTTCGTGAACGCGCAA
TTGAACAACATGCAAAGCCAATTGAATTAGATAGAGAAATTGTTGTTGTATCGCAAAATGATGAATTTACTTACCGTTAT
AAAGATTATGAATTAGAAACAATCATTTTAAGCATGTTAGGTGAACATCAGAAACAAAATGCTGCATTAGCCATAACAGC
TCTTATTGAATTAAATGAACAAGGATTAATTGAATTAGATTTCAATAAGATGATAGACGGTATTGAATCAGTTCGTTGGA
CTGGACGTATTGAGCAGGTGCATGACAAACCTTTAATCATTTTGGATGGCGCACATAATTCAGAGAGTATAGATGCTCTA
ATTGATACAATTAAACAGTACCATGATAAAGAAAAAGTAGATATTTTGTTCTCAGCAATAAACGGAAAACCGATTAACGA
GATGGTCAAACATTTAAGTTTAATTGCGCATACGTTTTATGCAACTGAATTTGATTTTCCGAAAGCGTTACGCAAAGAAG
AAATTGTAGGTAGTATTGAAAATGATGAAATACAATTAGTAGATGACTACGTTGAATTTATAAAAAATTATCAAGGTGAT
ACATTAGTAATTACCGGTAGTCTGTATTTCATAAGTGAAGTTAAATCAAAATTAGACTTTGAAGCATTATAA

Upstream 100 bases:

>100_bases
GGTTATAAATGAAGAAAAACGTAAAAAACAAGATTATCAAGAAAAATATGATGGTGTGAAGGCAAGAATTGAACAATTAA
AAGCATAGGAGTTAGTAACA

Downstream 100 bases:

>100_bases
AACTAAAACTAAAATAGCCATGAAGAATCTATTTAATCAATAGGTAGAGCTTCATGGTTATTTTTTATGCTCTAAATCGT
ATGTGTTTATAAGGTTCAAT

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolate synthase; Tetrahydrofolylpolyglutamate synthase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHVGGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFN
ERISLNGVPISNDAIVELVSRIKPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHAKPIELDREIVVVSQNDEFTYRY
KDYELETIILSMLGEHQKQNAALAITALIELNEQGLIELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDAL
IDTIKQYHDKEKVDILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVEFIKNYQGD
TLVITGSLYFISEVKSKLDFEAL

Sequences:

>Translated_423_residues
MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHVGGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFN
ERISLNGVPISNDAIVELVSRIKPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHAKPIELDREIVVVSQNDEFTYRY
KDYELETIILSMLGEHQKQNAALAITALIELNEQGLIELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDAL
IDTIKQYHDKEKVDILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVEFIKNYQGD
TLVITGSLYFISEVKSKLDFEAL
>Mature_423_residues
MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHVGGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFN
ERISLNGVPISNDAIVELVSRIKPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHAKPIELDREIVVVSQNDEFTYRY
KDYELETIILSMLGEHQKQNAALAITALIELNEQGLIELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDAL
IDTIKQYHDKEKVDILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVEFIKNYQGD
TLVITGSLYFISEVKSKLDFEAL

Specific function: Conversion of folates to polyglutamate derivatives. It preferes 5,10-methylenetetrahydrofolate, rather than 10- formyltetrahydrofolate as folate substrate [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=309, Percent_Identity=31.7152103559871, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI66932990, Length=309, Percent_Identity=31.7152103559871, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI1788654, Length=432, Percent_Identity=25.2314814814815, Blast_Score=115, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17553150, Length=337, Percent_Identity=28.1899109792285, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71984923, Length=300, Percent_Identity=29.3333333333333, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17553148, Length=337, Percent_Identity=28.1899109792285, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6323760, Length=431, Percent_Identity=27.1461716937355, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6324815, Length=306, Percent_Identity=28.7581699346405, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24641571, Length=291, Percent_Identity=31.9587628865979, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24581568, Length=177, Percent_Identity=28.8135593220339, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17 [H]

Molecular weight: Translated: 47857; Mature: 47857

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHVGGTNGKGSTVAYLRTA
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHH
LVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRIKPVSEMMERETDLGVATE
HHHCCCEECCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCHH
FEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPVLSILTSIGLDHTDILGGTYL
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCEE
DIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHAKPIELDREIVVVSQNDEFTYRY
EEECCCCCEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEE
KDYELETIILSMLGEHQKQNAALAITALIELNEQGLIELDFNKMIDGIESVRWTGRIEQV
ECCHHHHHHHHHHCCHHHCCCEEEEEEEEEECCCCEEEECHHHHHHHHHHHHHCCCHHHH
HDKPLIILDGAHNSESIDALIDTIKQYHDKEKVDILFSAINGKPINEMVKHLSLIAHTFY
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCCCCHHHHHHHHHHHHHHHH
ATEFDFPKALRKEEIVGSIENDEIQLVDDYVEFIKNYQGDTLVITGSLYFISEVKSKLDF
HCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCC
EAL
CCC
>Mature Secondary Structure
MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHVGGTNGKGSTVAYLRTA
CCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCHHHHHHHH
LVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRIKPVSEMMERETDLGVATE
HHHCCCEECCCCHHHHHHHHHHEEECCCCCCCHHHHHHHHHHCHHHHHHHHHCCCCCCHH
FEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPVLSILTSIGLDHTDILGGTYL
HHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCHHHCCCCEE
DIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHAKPIELDREIVVVSQNDEFTYRY
EEECCCCCEECCCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEE
KDYELETIILSMLGEHQKQNAALAITALIELNEQGLIELDFNKMIDGIESVRWTGRIEQV
ECCHHHHHHHHHHCCHHHCCCEEEEEEEEEECCCCEEEECHHHHHHHHHHHHHCCCHHHH
HDKPLIILDGAHNSESIDALIDTIKQYHDKEKVDILFSAINGKPINEMVKHLSLIAHTFY
CCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHHEEEEEECCCCCHHHHHHHHHHHHHHHH
ATEFDFPKALRKEEIVGSIENDEIQLVDDYVEFIKNYQGDTLVITGSLYFISEVKSKLDF
HCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHCCC
EAL
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8419299; 9384377; 2553669 [H]