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The map label for this gene is tnpB1

Identifier: 148268139

GI number: 148268139

Start: 1812794

End: 1814686

Strand: Reverse

Name: tnpB1

Synonym: SaurJH9_1716

Alternate gene names: 148268139

Gene position: 1814686-1812794 (Counterclockwise)

Preceding gene: 148268140

Following gene: 148268138

Centisome position: 62.43

GC content: 33.86

Gene sequence:

>1893_bases
ATGAATGCTTCTAGTAAAAGGAAAATTATTAGTCAGAGTGAGATTAGCAAAAAAATAGCTGTAATGAATGAAGAAATGCA
GGGGTTTTGGGCTAATAATAGTTGGGATATAAGAAAATGTCCACATCCTTCTGCCATAGAATTAAGTAAGAATCCTGCTT
TAAGGAATCGTTGGGTTCGTTTTGAACGTGTTAAAAATCTGTGGTTAAGAACAGAATTGAAATATTTTTATTTTTACCAT
TTAAACAATGGAATATGGAATGCAAAAACTGTCTGGATTAGAAAAGGAACAGTAATTAATAAAATGTTAGATTTCTTAGA
TTTAAAGTATCCTAGCATTACTTCAATTACTGAAGTTCCTATTGAAAAAGCAATGACGGAGTATAGAACTTATTTGACAA
AACGGGGTGTTAGAATTACCACTACTAATTATAAGATTACTGCTAATCAAGAAAAAACACCTGTAAAAGCTAATTCCTAC
TATGTTACTAATCTAAAACAATTTATGGAGTTTTATGAGAACTTTTATTTTGATGGAGAGGAGTGGGATAAAGACGTTTG
GGATAGACGTAACTTACCTTTGCCAGATGATAAGGTTAACCCAACACAATATGAATATACAATTAACTTTAAAGGGTTTC
GGAATACATATTTTAAACAACTTGTAAAAAGATATTGTAAGTTGAGATTGAACGTGGATAGCTTTTCCTATGTAAGTGAT
ATTGCCCAAAGACTTAAAGAGTTCTTTAATTTTCTGGACATGAAATTTAAACAAGTTCAGAGAGTACACCAATTAACGAG
AGTGGAAATTGAAGCATATTTAAGTGAACTAAACATGATGGGAATAAAACCTAGTACAATAACTGGGAGGATCTCTATAT
TGGAAGGACTATTTAGTACCCTTCTTAGGCTAGAATGGGATGATGTTCCTTCCAAAATATTAATTTATTCTGAGGACTAT
CCGAAAATACCAAGAGCAAAACCACGCTTTATAGATGAATTCGTCCTAGAGCAATTGAACAGTCATCTTGATAAATTACC
CGAATATATAGCTACGATGACTATGATTGTTCAAGAATGTGGAATGAGGATAAGTGAATTGTGCACCTTGAAAAAAGGCT
GTCTATTAGAGGACAAAGATGGAGATTTCTTTTTAAAGTATTATCAATGGAAAATGAAAAAGGAGCATATAGTTCCAATA
TCTAAAGAGGTAGCTTTACTTATTAAAGTTCGGGAAGATAAAGTTTCAGAGGAATTTCCAGATAGTGAATACCTCTTTCC
AAGAAAAGATGGATCGCCATTAAAACAAGAAACATTTAGAGGTGAGTTAAATAAATTAGCTTATGAGCAAAATATAGTGG
ATAAATCAGGTGAGATTTATAGATTCCATGCCCATGCCTTTCGCCATACAGTAGGAACAAGAATGATTAACAACGGGATG
CCCCAGCATATTGTGCAGAAATTTTTGGGGCATGAAAGCCCAGAAATGACAAGCAGATACGCTCATATCTTTGATGAAAC
TCTAAAAAATGAATTTACTAAATTTCAGGAAAAACTGGTTACCAATAATGGAGATGTGCTTGATCTAGATGAAGATAATG
AAGTCGATGATGTAGAGCTTCAATGGTTCAAGAAAAATATAAATGCACAAGTGCTTCCAAATGGTTATTGTAGATTGCCA
GTAGTAGCAGGTGGTTGTCCACATGCGAATGCATGCTTAGATTGCACTCACTTCTGTACCAGTAAGCAATTCTTACCACA
GCACGAAGAACAGTTAGAGCGTACAGAAGAGTTATTAGCCATAGCTAAGGATAAACAATGGCAAAGACAAGTAGAGACTA
ATAGCCGTGTTAAAGAGCGTTTAGAACAAATCATTGGAAGTTTGACGGGGTAA

Upstream 100 bases:

>100_bases
ACGCACATGTTCAGACAACGTTAAATACCTATGTTCATCTTTCAGATCAGGATATGAAAAATGAGTTTAATAAATACCTC
GAGAGAAAGGAGCATAAGAA

Downstream 100 bases:

>100_bases
TTATCAATGGATAAACAAGTTAGAAATACAACAGAAATTGTACGTTTGGCGAAGCAGAAATCAAAAAAGACAAGGGAAAA
AGTAGACAAAGCGATTTCTA

Product: phage integrase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPSAIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYH
LNNGIWNAKTVWIRKGTVINKMLDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYFKQLVKRYCKLRLNVDSFSYVSD
IAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSELNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDY
PKIPRAKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPI
SKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGM
PQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLP
VVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG

Sequences:

>Translated_630_residues
MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPSAIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYH
LNNGIWNAKTVWIRKGTVINKMLDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYFKQLVKRYCKLRLNVDSFSYVSD
IAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSELNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDY
PKIPRAKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPI
SKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGM
PQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLP
VVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG
>Mature_630_residues
MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPSAIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYH
LNNGIWNAKTVWIRKGTVINKMLDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYFKQLVKRYCKLRLNVDSFSYVSD
IAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSELNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDY
PKIPRAKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPI
SKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGM
PQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLP
VVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQIIGSLTG

Specific function: One of three proteins encoded by transposon Tn554 required for its transposition

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'phage' integrase family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TNPB_STAAM (P0A053)

Other databases:

- EMBL:   BA000017
- EMBL:   BA000017
- RefSeq:   NP_370579.1
- RefSeq:   NP_372182.1
- ProteinModelPortal:   P0A053
- STRING:   P0A053
- EnsemblBacteria:   EBSTAT00000007707
- EnsemblBacteria:   EBSTAT00000007936
- GeneID:   1120015
- GeneID:   1121633
- GenomeReviews:   BA000017_GR
- GenomeReviews:   BA000017_GR
- KEGG:   sav:SAV0055
- KEGG:   sav:SAV1658
- eggNOG:   COG0582
- GeneTree:   EBGT00050000024184
- HOGENOM:   HBG440333
- OMA:   RMINNGV
- ProtClustDB:   CLSK884357
- BioCyc:   SAUR158878:SAV0055-MONOMER
- BioCyc:   SAUR158878:SAV1658-MONOMER
- InterPro:   IPR011010
- InterPro:   IPR013762
- InterPro:   IPR002104
- InterPro:   IPR023109
- InterPro:   IPR004107
- Gene3D:   G3DSA:1.10.150.130
- Gene3D:   G3DSA:1.10.443.10

Pfam domain/function: PF02899 Phage_integr_N; PF00589 Phage_integrase; SSF56349 DNA_brk_join_enz

EC number: NA

Molecular weight: Translated: 74440; Mature: 74440

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS00018 EF_HAND_1

Important sites: ACT_SITE 500-500

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPSAIELSKNPALRNRWVR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCEEECCCCHHHHHHHH
FERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKMLDFLDLKYPSITSITEVP
HHHHHHHHHHEEEEEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCHHHHH
IEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSYYVTNLKQFMEFYENFYFDGE
HHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCCCEECCCEEHHHHHHHHHHHHHHCCCCC
EWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYFKQLVKRYCKLRLNVDSFSYVSD
HHCHHHHCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHEECCCCHHHHHHH
IAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSELNMMGIKPSTITGRISILEGLFST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQEC
HHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMRISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPISKEVALLIKVREDKVSEEFP
CCHHHHHHHHHCCCEEECCCCCEEEEHHHHHHCCCCCCCCCCCEEEEEEEHHHHHHHHCC
DSEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCC
PQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVEL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHH
QWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLA
HHHHHCCCCEECCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IAKDKQWQRQVETNSRVKERLEQIIGSLTG
HHHCHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPSAIELSKNPALRNRWVR
CCCCHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCCCCCCCCCCCEEECCCCHHHHHHHH
FERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKMLDFLDLKYPSITSITEVP
HHHHHHHHHHEEEEEEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCHHHHH
IEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSYYVTNLKQFMEFYENFYFDGE
HHHHHHHHHHHHHHCCEEEEEEEEEEEECCCCCCEECCCEEHHHHHHHHHHHHHHCCCCC
EWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYFKQLVKRYCKLRLNVDSFSYVSD
HHCHHHHCCCCCCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHHHHHEECCCCHHHHHHH
IAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSELNMMGIKPSTITGRISILEGLFST
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
LLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEFVLEQLNSHLDKLPEYIATMTMIVQEC
HHHCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GMRISELCTLKKGCLLEDKDGDFFLKYYQWKMKKEHIVPISKEVALLIKVREDKVSEEFP
CCHHHHHHHHHCCCEEECCCCCEEEEHHHHHHCCCCCCCCCCCEEEEEEEHHHHHHHHCC
DSEYLFPRKDGSPLKQETFRGELNKLAYEQNIVDKSGEIYRFHAHAFRHTVGTRMINNGM
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHCCC
PQHIVQKFLGHESPEMTSRYAHIFDETLKNEFTKFQEKLVTNNGDVLDLDEDNEVDDVEL
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHH
QWFKKNINAQVLPNGYCRLPVVAGGCPHANACLDCTHFCTSKQFLPQHEEQLERTEELLA
HHHHHCCCCEECCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
IAKDKQWQRQVETNSRVKERLEQIIGSLTG
HHHCHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11418146