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The map label for this gene is dnaG [H]

Identifier: 148268046

GI number: 148268046

Start: 1721491

End: 1723308

Strand: Reverse

Name: dnaG [H]

Synonym: SaurJH9_1620

Alternate gene names: 148268046

Gene position: 1723308-1721491 (Counterclockwise)

Preceding gene: 148268047

Following gene: 148268045

Centisome position: 59.29

GC content: 31.96

Gene sequence:

>1818_bases
TTGCGAATAGATCAATCGATCATTAATGAAATAAAAGATAAAACCGACATTTTAGACTTGGTAAGTGAATATGTAAAACT
AGAAAAGAGAGGACGCAATTATATAGGTTTGTGTCCTTTTCATGATGAAAAGACACCTTCATTTACAGTTTCTGAAGATA
AACAAATCTGTCATTGTTTTGGTTGTAAAAAAGGTGGCAATGTTTTTCAATTTACTCAAGAAATTAAAGACATATCATTT
GTTGAAGCGGTTAAAGAATTAGGTGATAGAGTTAATGTTGCTGTAGATATTGAGGCAACACAATCTAACTCAAATGTTCA
AATTGCTTCTGATGATTTACAAATGATTGAAATGCATGAGTTAATACAAGAATTTTATTATTACGCTTTAACAAAGACAG
TCGAAGGCGAACAAGCATTAACATACTTACAAGATCGTGGTTTTACAGATGCGCTTATTAAAGAGCGAGGCATTGGCTTT
GCACCCGATAGCTCACATTTTTGTCATGATTTTCTTCAAAAAAAGGGTTACGATATTGAATTAGCATATGAAGCCGGATT
ATTATCACGTAACGAAGAAAATTTCAGTTATTACGATAGATTTCGAAATCGTATTATGTTTCCTTTGAAAAATGCGCAAG
GAAGAATTGTTGGATATTCAGGTCGAACATATACCGGTCAAGAACCAAAATACCTAAATAGTCCTGAAACGCCTATCTTT
CAAAAAAGAAAGTTGTTATATAACTTAGATAAAGCACGTAAATCAATTAGAAAATTAGATGAAATTGTATTACTAGAAGG
TTTTATGGATGTTATAAAATCTGATACTGCTGGCTTGAAAAACGTTGTTGCAACAATGGGTACACAGTTGTCAGATGAAC
ATATTACCTTTATACGAAAGTTAACATCAAATATAACATTAATGTTTGATGGGGATTTTGCGGGTAGTGAAGCAACACTT
AAAACAGGTCAACATTTGTTACAGCAAGGGCTAAATGTATTTGTTATACAATTGCCATCTGGCATGGATCCGGATGAATA
CATTGGTAAGTATGGCAACGACGCATTTACTACTTTTGTAAAAAATGACAAAAAGTCATTTGCACATTATAAAGTAAGTA
TATTAAAAGATGAAATTGCACATAATGACCTTTCATATGAACGTTATTTGAAAGAACTGAGTCATGACATTTCACTTATG
AAGTCATCAATTCTGCAACAAAAGGCTATAAATGATGTTGCGCCATTTTTCAATGTTAGTCCTGAGCAGTTAGCTAACGA
AATACAATTCAATCAAGCACCAGCCAATTATTATCCAGAAGATGAGTATGGCGGTTATGATGAGTATGGCGGTTATATTG
AACCTGAGCCAATTGGTATGGCACAATTTGACAATTTGAGCCGTCAAGAAAAAGCGGAGCGAGCATTTTTAAAACATTTA
ATGAGAGATAAAGATACATTTTTAAATTATTATGAAAGTGTTGATAAGGATAACTTCACAAATCAGCATTTTAAATATGT
ATTCGAAGTCTTACATGATTTTTATGCGGAAAATGATCAATATAATATCAGTGATGCTGTGCAGTATGTTAATTCAAATG
AGTTGAGAGAAACACTAATTAGCTTAGAACAATATAATTTGAATGACGAACCATATGAAAATGAAATTGATGATTATGTC
AATGTTATTAATGAAAAAGGACAAGAAACAATTGAGTCATTGAATCATAAATTAAGGGAAGCTACAAGGATTGGCGATGT
AGAATTACAAAAATACTATTTACAGCAAATTGTTGCTAAGAATAAAGAACGCATGTAG

Upstream 100 bases:

>100_bases
CGATATAATCCATTATATTGAACAAAATAAATCGAAATGATTTCATTTTTGTCAAAAATTAGGTATAATAGTATAACTAA
TGCTTAATAGGTGATTTAAT

Downstream 100 bases:

>100_bases
CATGTGATTTTAAAGAATAATACGAATAATGATTATGTCAAAATGTATAAGGGTAAATGATAGTTACCGCATTTAAACAA
CACTATTGAAAAATAAATAT

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 605; Mature: 605

Protein sequence:

>605_residues
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISF
VEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQDRGFTDALIKERGIGF
APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATL
KTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTTFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLM
KSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHL
MRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYV
NVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM

Sequences:

>Translated_605_residues
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISF
VEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQDRGFTDALIKERGIGF
APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATL
KTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTTFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLM
KSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHL
MRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYV
NVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM
>Mature_605_residues
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISF
VEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQDRGFTDALIKERGIGF
APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATL
KTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTTFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLM
KSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHL
MRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYV
NVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=362, Percent_Identity=37.8453038674033, Blast_Score=243, Evalue=3e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007693
- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF00772 DnaB; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 70018; Mature: 70018

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECCCCHHHHHHH
GCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCHHHHHHHHH
LIQEFYYYALTKTVEGEQALTYLQDRGFTDALIKERGIGFAPDSSHFCHDFLQKKGYDIE
HHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEE
LAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
EEECCCCCCCCCCCHHHHHHHHHCEECEEECCCCCEEECCCCCCCCCCCCCCCCCCCCHH
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
LTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTTFV
HHCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCCHHHHHH
KNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLMKSSILQQKAINDVAPFFNVS
HCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEQLANEIQFNQAPANYYPEDEYGGYDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHL
HHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHH
MRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHH
SLEQYNLNDEPYENEIDDYVNVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAK
HHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
NKERM
HCCCC
>Mature Secondary Structure
MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEKTPSFTVSEDKQICHCF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECCCCHHHHHHH
GCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQSNSNVQIASDDLQMIEMHE
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEECCHHHHHHHHH
LIQEFYYYALTKTVEGEQALTYLQDRGFTDALIKERGIGFAPDSSHFCHDFLQKKGYDIE
HHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEE
LAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVGYSGRTYTGQEPKYLNSPETPIF
EEECCCCCCCCCCCHHHHHHHHHCEECEEECCCCCEEECCCCCCCCCCCCCCCCCCCCHH
QKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDTAGLKNVVATMGTQLSDEHITFIRK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHH
LTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVFVIQLPSGMDPDEYIGKYGNDAFTTFV
HHCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCCCCHHHHHH
KNDKKSFAHYKVSILKDEIAHNDLSYERYLKELSHDISLMKSSILQQKAINDVAPFFNVS
HCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PEQLANEIQFNQAPANYYPEDEYGGYDEYGGYIEPEPIGMAQFDNLSRQEKAERAFLKHL
HHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHH
MRDKDTFLNYYESVDKDNFTNQHFKYVFEVLHDFYAENDQYNISDAVQYVNSNELRETLI
HHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHH
SLEQYNLNDEPYENEIDDYVNVINEKGQETIESLNHKLREATRIGDVELQKYYLQQIVAK
HHHHCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
NKERM
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11418146 [H]