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The map label for this gene is nucI [H]

Identifier: 148267812

GI number: 148267812

Start: 1445969

End: 1446541

Strand: Direct

Name: nucI [H]

Synonym: SaurJH9_1385

Alternate gene names: 148267812

Gene position: 1445969-1446541 (Clockwise)

Preceding gene: 148267810

Following gene: 148267814

Centisome position: 49.75

GC content: 31.94

Gene sequence:

>573_bases
ATGATTAATCGGAATGAAAAAAGAGAAGTAGGTGGCAATATGAAGTCAAATAAATCGCTTGCTATGATTGTGGTAGCCAT
CATTATTGTAGGTGTATTAGCATTTCAATTTATGAATCATACGGGTCCTTTCAAAAAGGGGACGAATCATGAAACTGTAC
AAGATTTAAATGGTAAAGATAAAGTACATGTTCAAAGAGTTGTGGATGGTGATACATTTATTGCAAATCAAAATGGTAAA
GAAATTAAAGTTAGGCTTATAGGGGTTGATACGCCAGAAACGGTGAAACCGAATACGCCTGTACAACCATTTGGCAAAGA
AGCATCAAATTATAGTAAGAAGACATTAACAAATCAAGATGTTTATTTAGAATATGATAAAGAAAAACAAGATCGCTATG
GTAGAACATTGGCGTATGTATGGATAAGTAAAGATCGTATGTACAATAAGGAATTAGTGGAAAAGGGACTTGCTAGAGAG
AAGTATTTTTCACCAAATGGCAAATATAGAAATGTATTTATAGAAGCACAAAATAAAGCTAAACAACAGAAATTAAATAT
TTGGAGTAAATAA

Upstream 100 bases:

>100_bases
TTTATTTTCACTCCTTAACAATAACATTATATCATGCTATAGCTTTCCAAAATATTGAAATATGTAGATATGGCTATTGA
CGATATTTCTTAACTTTTAT

Downstream 100 bases:

>100_bases
TTAAATAGGTGATTATATATATTAAAGTAAAATATACTTGAAGCATCGATTTATTATTACCATAACTTTATATATAAAGA
AATAATTTAAAGGCATAATA

Product: thermonuclease

Products: NA

Alternate protein names: TNase; Micrococcal nuclease; Staphylococcal nuclease [H]

Number of amino acids: Translated: 190; Mature: 190

Protein sequence:

>190_residues
MINRNEKREVGGNMKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLNGKDKVHVQRVVDGDTFIANQNGK
EIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKTLTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLARE
KYFSPNGKYRNVFIEAQNKAKQQKLNIWSK

Sequences:

>Translated_190_residues
MINRNEKREVGGNMKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLNGKDKVHVQRVVDGDTFIANQNGK
EIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKTLTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLARE
KYFSPNGKYRNVFIEAQNKAKQQKLNIWSK
>Mature_190_residues
MINRNEKREVGGNMKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLNGKDKVHVQRVVDGDTFIANQNGK
EIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKTLTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLARE
KYFSPNGKYRNVFIEAQNKAKQQKLNIWSK

Specific function: Enzyme that catalyzes the hydrolysis of both DNA and RNA at the 5'-position of the phosphodiester bond [H]

COG id: COG1525

COG function: function code L; Micrococcal nuclease (thermonuclease) homologs

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thermonuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016071
- InterPro:   IPR006021
- InterPro:   IPR002071 [H]

Pfam domain/function: PF00565 SNase [H]

EC number: =3.1.31.1 [H]

Molecular weight: Translated: 21827; Mature: 21827

Theoretical pI: Translated: 10.32; Mature: 10.32

Prosite motif: PS01284 TNASE_2 ; PS50830 TNASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINRNEKREVGGNMKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLNGKD
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCC
KVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKTLTNQD
CEEEEEEECCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCC
VYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVFIEAQNKA
EEEEECCHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCHH
KQQKLNIWSK
HHHHHCCCCC
>Mature Secondary Structure
MINRNEKREVGGNMKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLNGKD
CCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCCCC
KVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKTLTNQD
CEEEEEEECCCEEEECCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCC
VYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVFIEAQNKA
EEEEECCHHHHHHCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEEEECCHH
KQQKLNIWSK
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1408843 [H]