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The map label for this gene is trmD

Identifier: 148267731

GI number: 148267731

Start: 1349652

End: 1350389

Strand: Direct

Name: trmD

Synonym: SaurJH9_1299

Alternate gene names: 148267731

Gene position: 1349652-1350389 (Clockwise)

Preceding gene: 148267730

Following gene: 148267732

Centisome position: 46.43

GC content: 35.5

Gene sequence:

>738_bases
ATGAAAATTGATTATTTAACTTTATTTCCTGAAATGTTTGATGGTGTTTTAAATCATTCAATTATGAAACGTGCCCAAGA
AAACAATAAATTACAAATCAATACGGTTAATTTTAGAGATTATGCAATTAACAAGCACAACCAAGTAGATGATTATCCGT
ATGGTGGCGGACAAGGTATGGTGTTAAAGCCTGAACCTGTTTTTAATGCGATGGAAGACTTAGATGTCACAGAACAAACA
CGCGTTATTTTAATGTGTCCACAAGGCGAGCCATTTTCACATCAGAAAGCTGTTGAATTAAGCAAGGCTGACCACATCGT
TTTCATATGCGGACATTATGAAGGTTACGATGAACGTATCCGAACACATCTTGTCACAGATGAAATATCAATGGGTGACT
ATGTGTTAACTGGCGGTGAGTTACCGGCAATGACAATGACTGATGCTATTGTTAGACTGATTCCAGGTGTTTTAGGTAAT
GAACAGTCACATCAAGACGATTCATTTTCAGATGGGTTATTAGAGTTTCCGCAATATACACGTCCGCGTGAATTTAAGGG
TCTAACAGTTCCAGATGTTTTATTGTCTGGAAATCATGCCAATATTGATGCATGGAGACATGAGCAAAAGTTGATCCGCA
CATATAATAAAAGACCTGACTTAATTGAAAAATATCCATTAACTAATGAAGATAAGCAAATATTAGAAAGATATAAAATA
GGATTGAAAAAAGGTTAG

Upstream 100 bases:

>100_bases
CTAAAGAATATTTGATTCCTTATATTGCTGATGTTGTAAAAGAAGTGGATGTTGAAAATAAAAAAATTATCATCACGCCA
ATGGAAGGATTGTTGGATTA

Downstream 100 bases:

>100_bases
TATTGTGCTAACATATTCTGAGTGTGGAAACACAAAAATCACTATGATCCGCTGCTATATATTTGTCGAGGCAAGAACAT
AGGTTGAAGAGGAGAATTTA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQT
RVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNEDKQILERYKI
GLKKG

Sequences:

>Translated_245_residues
MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQT
RVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNEDKQILERYKI
GLKKG
>Mature_245_residues
MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQT
RVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNEDKQILERYKI
GLKKG

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=241, Percent_Identity=43.9834024896266, Blast_Score=219, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_STAA1 (A7X1L0)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441820.1
- ProteinModelPortal:   A7X1L0
- SMR:   A7X1L0
- STRING:   A7X1L0
- EnsemblBacteria:   EBSTAT00000004527
- GeneID:   5560064
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1230
- eggNOG:   COG0336
- GeneTree:   EBGT00050000024591
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   SAUR418127:SAHV_1230-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28114; Mature: 28114

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGM
CCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCE
VLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERI
EECCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCEEEEEEECCCCHHHHH
RTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGNEQSHQDDSFSDGLLEFPQYT
HHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCC
RPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNEDKQILERYKI
CCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHH
GLKKG
CCCCC
>Mature Secondary Structure
MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAINKHNQVDDYPYGGGQGM
CCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCCE
VLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSKADHIVFICGHYEGYDERI
EECCCHHHHHHHHCCCCCCCEEEEECCCCCCCCCHHHHHHCCCCEEEEEEECCCCHHHHH
RTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGNEQSHQDDSFSDGLLEFPQYT
HHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCC
RPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKRPDLIEKYPLTNEDKQILERYKI
CCCCCCCCCCCHHEECCCCCCCHHHHHHHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHHH
GLKKG
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA