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The map label for this gene is rlmN

Identifier: 148267709

GI number: 148267709

Start: 1325121

End: 1326215

Strand: Direct

Name: rlmN

Synonym: SaurJH9_1277

Alternate gene names: 148267709

Gene position: 1325121-1326215 (Clockwise)

Preceding gene: 148267708

Following gene: 148267710

Centisome position: 45.59

GC content: 33.88

Gene sequence:

>1095_bases
ATGATAACTGCTGAAAAGAAAAAGAAGAATAAATTTCTTCCAAATTTTGACAAGCAATCAATATATTCATTGCGATTTGA
CGAAATGCAAAACTGGCTCGTTGAACAAGGTCAACAAAAATTTCGAGCGAAACAGATTTTTGAATGGTTATATCAAAAAA
GAGTAGATTCGATTGATGAAATGACGAACTTATCGAAAGACTTACGACAGCTTTTAAAAGATAACTTTACTGTTACAACT
TTAACAACTGTAGTAAAACAAGAAAGTAAAGACGGTACAATTAAATTCTTATTTGAATTACAAGATGGCTATACAATTGA
AACTGTTTTAATGAGACATGATTATGGAAATTCAGTATGTGTAACGACACAAGTAGGTTGTCGTATCGGATGTACGTTTT
GTGCTTCTACACTTGGCGGCTTAAAAAGAAACCTTGAAGCTGGCGAAATTGTTTCACAAGTTTTAACAGTTCAAAAAGCC
CTTGATGCTACAGAAGAGCGCGTATCTCAAATTGTCATAATGGGTATCGGTGAACCATTTGAAAATTATGATGAAATGAT
GGACTTTTTAAGAATCGTCAATGATGATAACAGTTTAAATATTGGTGCACGTCACATTACAGTATCAACATCAGGTATCA
TTCCTAGAATATATGACTTTGCGGATGAAGATATCCAAATTAATTTTGCTGTAAGCTTACACGCCGCAAAAGATGAAGTG
CGATCACGCTTGATGCCAATTAACCGTGCATATAATGTTGAGAAGTTAATCGAAGCAATTCAATATTATCAAGAAAAAAC
AAATCGTCGTGTTACTTTTGAATATGGTCTGTTTGGTGGTGTGAATGACCAACTAGAACATGCAAGAGAATTAGCACATT
TAATAAAAGGCTTAAACTGCCATGTTAACTTAATTCCTGTCAACCATGTTCCAGAAAGAAATTATGTGAAAACGGCTAAA
AATGATATCTTTAAATTTGAAAAAGAATTAAAGAGACTAGGAATTAATGCCACAATACGTCGTGAACAAGGTTCGGATAT
TGACGCAGCTTGTGGTCAATTAAGAGCAAAGGAACGACAAGTAGAAACGAGGTAA

Upstream 100 bases:

>100_bases
CGATAACTGGAGAGTTGGTCAAAACGTTACAAATCATGCCGCAAGACTTTAATTCAGATGGATTCTTTATCACTAAGATA
AAAAGAAAGGACAATTAGGA

Downstream 100 bases:

>100_bases
AGACAAATGCTAGAGGCACAATTTTTTACTGATACTGGACAACATAGAGATAAGAATGAAGATGCGGGTGGTATTTTTTA
TAATCAAACTAATCAACAAC

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTT
LTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAK
NDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQVETR

Sequences:

>Translated_364_residues
MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTT
LTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAK
NDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQVETR
>Mature_364_residues
MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTT
LTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAK
NDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQVETR

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA. Confers resistance to some classes of antibiotics

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=344, Percent_Identity=37.5, Blast_Score=251, Evalue=6e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_STAA1 (A7X1H8)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441798.1
- ProteinModelPortal:   A7X1H8
- STRING:   A7X1H8
- EnsemblBacteria:   EBSTAT00000002848
- GeneID:   5561252
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1208
- eggNOG:   COG0820
- GeneTree:   EBGT00050000024137
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14455
- BioCyc:   SAUR418127:SAHV_1208-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 41905; Mature: 41905

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVC
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEEECCCCCCEE
VTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKALDATEERVSQIVIMGIGEPF
EEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCC
ENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
CHHHHHHHHHHHHCCCCCEEECEEEEEEECCCCCHHHHHCCCCCEEEEEEEEEEHHHHHH
RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNC
HHHHCCCHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
HVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQ
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHH
VETR
CCCC
>Mature Secondary Structure
MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAKQIFEWLYQKRVDSIDE
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQDGYTIETVLMRHDYGNSVC
HHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCEEEEEEEECCCCCCEE
VTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKALDATEERVSQIVIMGIGEPF
EEEECCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCC
ENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIYDFADEDIQINFAVSLHAAKDEV
CHHHHHHHHHHHHCCCCCEEECEEEEEEECCCCCHHHHHCCCCCEEEEEEEEEEHHHHHH
RSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYGLFGGVNDQLEHARELAHLIKGLNC
HHHHCCCHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCC
HVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLGINATIRREQGSDIDAACGQLRAKERQ
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHH
VETR
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA