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The map label for this gene is carA

Identifier: 148267693

GI number: 148267693

Start: 1306616

End: 1307716

Strand: Direct

Name: carA

Synonym: SaurJH9_1261

Alternate gene names: 148267693

Gene position: 1306616-1307716 (Clockwise)

Preceding gene: 148267692

Following gene: 148267694

Centisome position: 44.95

GC content: 35.33

Gene sequence:

>1101_bases
ATGCAAAGCAAACGTTATCTAGTGTTAGAAGACGGTTCTTTTTACGAAGGCTACCGTTTAGGATCTGATAACTTAACTGT
AGGAGAAATTGTATTTAATACAGCTATGACAGGTTATCAAGAAACTATTTCAGATCCATCATATACAGGTCAGATCATTA
CTTTTACGTATCCATTAATCGGTAATTATGGTATCAATAGAGACGATTTTGAATCATTAGTACCTACATTAAACGGTATT
GTAGTGAAAGAAGCGAGTGCGCATCCAAGTAATTTTAGACAACAAAAGACACTTCATGACGTTTTAGAATTGCATCAAAT
TCCAGGGATTGCAGGTGTTGATACAAGAAGTATTACGCGTAAAATTCGACAACACGGTGTGTTAAAAGCTGGTTTTACTG
ATCGAAAAGAAGATATTGATCAACTTGTCAAACATTTACAACAAGTAGAATTACCTAAAAACGAAGTAGAAATCGTTTCG
ACTAAAACACCGTATGTTTCGACAGGTAAGGATCTAAGTGTCGTACTTGTAGACTTTGGTAAGAAGCAAAATATTGTTCG
AGAATTAAACGTCAGAGGTTGTAACGTCACAGTTGTACCATATACAACTACTGCCGAAGAAATTTTAGCAATGGCTCCAG
ATGGCGTTATGCTATCAAACGGACCAGGTAATCCTGAAGTTGTAGAATGTGCGATTCCAATGATTCAAGGAATTTTAGGG
AAAATTCCGTTCTTTGGTATCTGTCTAGGACATCAACTTTTTGCATTATCTCAAGGAGCAAGCTCATTTAAAATGAAGTT
TGGTCATCGTGGTGCGAACCATCCAGTTAAAAATTTAGAGACTGGAAAAGTTGATATTACGAGTCAAAACCATGGATATG
CAATAGATATAGATTCGTTAAAAAGTACTGATTTAGAAGTTACTCATCTTGCATTAAATGATGGTACTGTAGAAGGTTTA
AAACATAAAACATTACCAGCATTTTCTGTTCAATACCATCCTGAAGCAAATCCAGGACCGTCAGATTCAAACTATCTATT
TGATGATTTTGTAGCAATGATGACTAATTTTAAGGAAAAGGAGCGTCATATCAATGCCTAA

Upstream 100 bases:

>100_bases
TCAAAAGCAGATAATACACCATTTATCGGCTATAAAGTTTATGGAAATCCGATCTTAACAATGGTTGAAGGCGAAGTTAA
ATTTGAGGGGGATAAATAAT

Downstream 100 bases:

>100_bases
ACGTAATGATATCAAAACAATTTTAGTAATAGGGTCTGGGCCAATTATCATAGGTCAAGCAGCTGAATTTGATTATGCTG
GAACACAAGCATGTCTAGCT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 366; Mature: 366

Protein sequence:

>366_residues
MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETISDPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGI
VVKEASAHPSNFRQQKTLHDVLELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVMLSNGPGNPEVVECAIPMIQGILG
KIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPVKNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGL
KHKTLPAFSVQYHPEANPGPSDSNYLFDDFVAMMTNFKEKERHINA

Sequences:

>Translated_366_residues
MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETISDPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGI
VVKEASAHPSNFRQQKTLHDVLELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVMLSNGPGNPEVVECAIPMIQGILG
KIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPVKNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGL
KHKTLPAFSVQYHPEANPGPSDSNYLFDDFVAMMTNFKEKERHINA
>Mature_366_residues
MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETISDPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGI
VVKEASAHPSNFRQQKTLHDVLELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVMLSNGPGNPEVVECAIPMIQGILG
KIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPVKNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGL
KHKTLPAFSVQYHPEANPGPSDSNYLFDDFVAMMTNFKEKERHINA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=358, Percent_Identity=42.4581005586592, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI169790915, Length=367, Percent_Identity=36.7847411444142, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI21361331, Length=367, Percent_Identity=36.7847411444142, Blast_Score=219, Evalue=3e-57,
Organism=Escherichia coli, GI1786215, Length=385, Percent_Identity=44.9350649350649, Blast_Score=302, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI193204318, Length=361, Percent_Identity=38.781163434903, Blast_Score=245, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6324878, Length=367, Percent_Identity=40.8719346049046, Blast_Score=253, Evalue=5e-68,
Organism=Saccharomyces cerevisiae, GI6322331, Length=394, Percent_Identity=37.5634517766497, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI45555749, Length=382, Percent_Identity=39.2670157068063, Blast_Score=254, Evalue=5e-68,
Organism=Drosophila melanogaster, GI24642586, Length=382, Percent_Identity=39.2670157068063, Blast_Score=254, Evalue=7e-68,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_STAAC (Q5HGN0)

Other databases:

- EMBL:   CP000046
- RefSeq:   YP_186077.1
- ProteinModelPortal:   Q5HGN0
- SMR:   Q5HGN0
- STRING:   Q5HGN0
- EnsemblBacteria:   EBSTAT00000008355
- GeneID:   3237657
- GenomeReviews:   CP000046_GR
- KEGG:   sac:SACOL1214
- TIGR:   SACOL1214
- eggNOG:   COG0505
- GeneTree:   EBGT00050000024835
- HOGENOM:   HBG286341
- OMA:   FTYPELG
- ProtClustDB:   PRK12564
- BioCyc:   SAUR93062:SACOL1214-MONOMER
- HAMAP:   MF_01209_B
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 40394; Mature: 40394

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 248-248 ACT_SITE 333-333 ACT_SITE 335-335

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETISDPSYTGQIITFTYPLI
CCCCCEEEEECCCCCCCEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEEEEC
GNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVLELHQIPGIAGVDTRSITR
CCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
KIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVSTKTPYVSTGKDLSVVLVDFG
HHHHCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEECCCCCEEEEEECC
KKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVMLSNGPGNPEVVECAIPMIQGILG
CCHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
KIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPVKNLETGKVDITSQNHGYAIDIDSL
CCCHHHHHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCC
KSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHPEANPGPSDSNYLFDDFVAMMTNFKEK
CCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCHHHH
ERHINA
HHCCCC
>Mature Secondary Structure
MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETISDPSYTGQIITFTYPLI
CCCCCEEEEECCCCCCCEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCEEEEEEEEEC
GNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVLELHQIPGIAGVDTRSITR
CCCCCCHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
KIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVSTKTPYVSTGKDLSVVLVDFG
HHHHCCCEECCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCEECCCCCEEEEEECC
KKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVMLSNGPGNPEVVECAIPMIQGILG
CCHHHHHHHCCCCCEEEEEECCCCHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHH
KIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPVKNLETGKVDITSQNHGYAIDIDSL
CCCHHHHHHHHHHHHHHCCCCHHEEECCCCCCCCCCCCCCCCEEEEEECCCCEEEEECCC
KSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHPEANPGPSDSNYLFDDFVAMMTNFKEK
CCCCEEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHCHHHH
ERHINA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA