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The map label for this gene is murD

Identifier: 148267675

GI number: 148267675

Start: 1286125

End: 1287474

Strand: Direct

Name: murD

Synonym: SaurJH9_1242

Alternate gene names: 148267675

Gene position: 1286125-1287474 (Clockwise)

Preceding gene: 148267674

Following gene: 148267676

Centisome position: 44.25

GC content: 32.59

Gene sequence:

>1350_bases
ATGCTTAATTATACAGGGTTAGAAAATAAAAATGTATTAGTCGTCGGTTTGGCAAAAAGTGGTTATGAAGCAGCTAAATT
ATTAAGTAAATTAGGTGCGAATGTGACTGTCAATGATGGGAAAGACTTATCGCAAGATGCTCATGCAAAAGATTTAGAAT
CTATGGGTATTTCTGTTGTAAGTGGAAGTCATCCATTAACATTGCTTGATAATAATCCAATAATTGTTAAAAATCCTGGA
ATACCTTATACAGTATCTATTATTGATGAAGCAGTGAAACGAGGTTTGAAAATTTTAACAGAAGTTGAGTTAAGTTATCT
AATCTCTGAAGCACCAATCATAGCTGTAACGGGTACAAATGGTAAAACGACAGTTACTTCTCTAATTGGAGATATGTTTA
AAAAAAGTCGCTTAACTGGAAGATTATCCGGCAATATTGGTTATGTTGCATCTAAAGTAGCACAAGAAGTAAAGCCTACA
GATTATTTAGTTACAGAGTTGTCGTCATTCCAGTTACTTGGAATTGAAAAGTATAAACCACACATTGCTATAATTACTAA
CATTTATTCGGCGCATCTAGATTACCATGAAAATTTAGAAAACTATCAAAATGCTAAAAAGCAAATATATAAAAATCAAA
CGGAAGAAGATTATTTGATTTGTAATTATCATCAAAGACAAGTGATAGAGTCGGAAGAATTAAAAGCTAAGACATTGTAT
TTCTCAACTCAACAAGAAGTTGATGGTATTTATATTAAAGATGGTTTTATCGTTTATAAAGGTGTTCGTATTATTAACAC
TGAAGATCTAGTATTGCCTGGTGAACATAATTTAGAAAATATATTAGCAGCTGTGCTTGCTTGTATTTTAGCTGGTGTAC
CTATTAAAGCAATTATTGATAGTTTAACTACATTTTCAGGAATAGAGCATAGATTGCAATATGTTGGTACTAATAGAACT
AATAAATATTATAATGATTCCAAAGCAACAAACACGCTAGCAACACAGTTTGCCTTAAATTCATTTAATCAACCAATCAT
TTGGTTATGTGGTGGTTTGGATCGAGGGAATGAATTTGACGAACTCATTCCTTATATGGAAAATGTTCGCGCGATGGTTG
TATTCGGACAAACGAAAGCTAAGTTTGCTAAACTAGGTAATAGTCAAGGGAAATCGGTCATTGAAGCGAACAATGTCGAA
GACGCTGTTGATAAAGTACAAGATATTATAGAACCAAATGATGTTGTATTATTGTCACCTGCTTGTGCGAGTTGGGATCA
ATATAGTACTTTTGAAGAGCGTGGAGAGAAATTTATTGAAAGATTCCGTGCCCATTTACCATCTTATTAA

Upstream 100 bases:

>100_bases
TTTGAATTGATAGGATGGAGCGAATGGAAAGTAGTTACAGTATTTTGGGCTGTTGGTCTGATTTCAGGTTTAATCGGTTT
ATGGATTGGAGTGCATTAAG

Downstream 100 bases:

>100_bases
AGGGTGTGAGTATTGATGGATGATAAAACGAAGAACGATCAACAAGAATCAAATGAAGATAAAGATGAATTAGAATTATT
TACGAGGAATACATCTAAGA

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLSQDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPG
IPYTVSIIDEAVKRGLKILTEVELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDYLICNYHQRQVIESEELKAKTLY
FSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEHNLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRT
NKYYNDSKATNTLATQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKSVIEANNVE
DAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY

Sequences:

>Translated_449_residues
MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLSQDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPG
IPYTVSIIDEAVKRGLKILTEVELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDYLICNYHQRQVIESEELKAKTLY
FSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEHNLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRT
NKYYNDSKATNTLATQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKSVIEANNVE
DAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY
>Mature_449_residues
MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLSQDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPG
IPYTVSIIDEAVKRGLKILTEVELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDYLICNYHQRQVIESEELKAKTLY
FSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEHNLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRT
NKYYNDSKATNTLATQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKSVIEANNVE
DAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=453, Percent_Identity=30.9050772626932, Blast_Score=179, Evalue=3e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_STAA1 (A7X1C4)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441763.1
- ProteinModelPortal:   A7X1C4
- STRING:   A7X1C4
- EnsemblBacteria:   EBSTAT00000005072
- GeneID:   5561308
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_1173
- eggNOG:   COG0771
- GeneTree:   EBGT00050000023807
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- ProtClustDB:   PRK02472
- BioCyc:   SAUR418127:SAHV_1173-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 49844; Mature: 49844

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLSQDAHAKDLESMGISVV
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHCCEEEE
SGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEVELSYLISEAPIIAVTGTN
ECCCCEEEECCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
GKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPTDYLVTELSSFQLLGIEKYKP
CCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHCCCCHHHHHHHCCEEEEEECCCCC
HIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDYLICNYHQRQVIESEELKAKTLY
CEEEEEHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHCHHHCEEEEEE
FSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEHNLENILAAVLACILAGVPIKAIID
EECHHCCCEEEEECCEEEEECEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHH
SLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLATQFALNSFNQPIIWLCGGLDRGNEFD
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHH
ELIPYMENVRAMVVFGQTKAKFAKLGNSQGKSVIEANNVEDAVDKVQDIIEPNDVVLLSP
HHHHHHHCCEEEEEECCCHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEECC
ACASWDQYSTFEERGEKFIERFRAHLPSY
CCCCCHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLSQDAHAKDLESMGISVV
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHCCCEEECCCCCCCCCHHHHHHHHCCEEEE
SGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEVELSYLISEAPIIAVTGTN
ECCCCEEEECCCCEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCC
GKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPTDYLVTELSSFQLLGIEKYKP
CCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHCCCCHHHHHHHCCEEEEEECCCCC
HIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDYLICNYHQRQVIESEELKAKTLY
CEEEEEHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEECHHHHHHCHHHCEEEEEE
FSTQQEVDGIYIKDGFIVYKGVRIINTEDLVLPGEHNLENILAAVLACILAGVPIKAIID
EECHHCCCEEEEECCEEEEECEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHH
SLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLATQFALNSFNQPIIWLCGGLDRGNEFD
HHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHH
ELIPYMENVRAMVVFGQTKAKFAKLGNSQGKSVIEANNVEDAVDKVQDIIEPNDVVLLSP
HHHHHHHCCEEEEEECCCHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHCCCCCEEEECC
ACASWDQYSTFEERGEKFIERFRAHLPSY
CCCCCHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA