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The map label for this gene is potB [H]

Identifier: 148267593

GI number: 148267593

Start: 1202532

End: 1203329

Strand: Direct

Name: potB [H]

Synonym: SaurJH9_1160

Alternate gene names: 148267593

Gene position: 1202532-1203329 (Clockwise)

Preceding gene: 148267592

Following gene: 148267594

Centisome position: 41.37

GC content: 27.32

Gene sequence:

>798_bases
ATGCGTAATACTAATAAATTTCTCTTAATCCCGTATTTACTATGGATGGTTATATTTATTATTGTACCAGTTGTATTACT
CATTTATTTTTCATTTTTAGATATCAATGGACATTTTAGTTTCACGAATTATCAACAAATTTTTACTACAAAATATTTGA
AAATGTTTGCATATTCAATTTTATATGCCGCTTTAATAACAATTATTACGTTGGCTATCAGTTATCCAGCTGCCTATTAT
ATTACTCGTTCGAAATTTCAAAATATCTTATTAATGATAATGATTATTCCAACATGGATAAATTTATTGTTAAAGACATA
TGCTTTTATAGGTTTATTAAGTCATGATGGCGTGATTAATCAATTTTTCCACTTATTTAATTTACCATCATTCAATTTGT
TATTTACAACTGGTGCGTTTTTAGTAGTGGCAAGTTACATTTATATACCATTTATGATTTTACCTATATTTAATAGCATG
AAAGCAATTCCTAATAATTTATTGCAGGCCTCAAGTGATTTAGGTGCCAGTCCTTTCTATACTTTTAGAAAAGTAATCAT
GCCGTTAACAAAAGAAGGTGTTATGACTGGGATTCAAGTAACCTTTATTCCATCACTTTCATTATTTATGATTACAAGAT
TAATTGCAGGTAATAAAGTCATAAATATAGGTACGGCAATAGAGGAACAATTTTTAACAATTCAAAATTATGGTATGGGA
TCAACTATAGCTATATTCTTAATTGTATTTATGGCATTCATTTTAATCATTACAAAATCATCTAATGGGAGAGGGTGA

Upstream 100 bases:

>100_bases
GTTGGTCTTTATTTTGATCCTGAAGCCATTCATATTATGGTTCCTGGAGAAACAGAAGAAGAATTTGATAAACGTATTGA
AAGCTATGAGGAAGTAGACA

Downstream 100 bases:

>100_bases
ATCATATGAAATGGTATGGAAAGCTGTATATCGGGATACTTTTAGCGATTTTATACATCCCAATATTCTTTTTAATGTTC
TATTCATTCAATTCGGCTGG

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNYQQIFTTKYLKMFAYSILYAALITIITLAISYPAAYY
ITRSKFQNILLMIMIIPTWINLLLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGNKVINIGTAIEEQFLTIQNYGMG
STIAIFLIVFMAFILIITKSSNGRG

Sequences:

>Translated_265_residues
MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNYQQIFTTKYLKMFAYSILYAALITIITLAISYPAAYY
ITRSKFQNILLMIMIIPTWINLLLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGNKVINIGTAIEEQFLTIQNYGMG
STIAIFLIVFMAFILIITKSSNGRG
>Mature_265_residues
MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNYQQIFTTKYLKMFAYSILYAALITIITLAISYPAAYY
ITRSKFQNILLMIMIIPTWINLLLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSM
KAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGNKVINIGTAIEEQFLTIQNYGMG
STIAIFLIVFMAFILIITKSSNGRG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=253, Percent_Identity=30.4347826086957, Blast_Score=140, Evalue=7e-35,
Organism=Escherichia coli, GI1787080, Length=225, Percent_Identity=33.7777777777778, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1787713, Length=243, Percent_Identity=30.0411522633745, Blast_Score=101, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30147; Mature: 30147

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNYQQIFTTKYLKMFAYSI
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LYAALITIITLAISYPAAYYITRSKFQNILLMIMIIPTWINLLLKTYAFIGLLSHDGVIN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
QFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSMKAIPNNLLQASSDLGASPFY
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
TFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGNKVINIGTAIEEQFLTIQNYGMG
HHHHHHHHHHHHHHHHCEEEEEECHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCC
STIAIFLIVFMAFILIITKSSNGRG
HHHHHHHHHHHHHHHHEECCCCCCC
>Mature Secondary Structure
MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNYQQIFTTKYLKMFAYSI
CCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
LYAALITIITLAISYPAAYYITRSKFQNILLMIMIIPTWINLLLKTYAFIGLLSHDGVIN
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
QFFHLFNLPSFNLLFTTGAFLVVASYIYIPFMILPIFNSMKAIPNNLLQASSDLGASPFY
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHH
TFRKVIMPLTKEGVMTGIQVTFIPSLSLFMITRLIAGNKVINIGTAIEEQFLTIQNYGMG
HHHHHHHHHHHHHHHHCEEEEEECHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHCCCCC
STIAIFLIVFMAFILIITKSSNGRG
HHHHHHHHHHHHHHHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]