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The map label for this gene is purF [H]

Identifier: 148267563

GI number: 148267563

Start: 1171644

End: 1173128

Strand: Direct

Name: purF [H]

Synonym: SaurJH9_1130

Alternate gene names: 148267563

Gene position: 1171644-1173128 (Clockwise)

Preceding gene: 148267562

Following gene: 148267564

Centisome position: 40.31

GC content: 35.82

Gene sequence:

>1485_bases
ATGTTTAACTACTCAGGATTAAACGAAGAATGTGGCGTGTTTGGTATTTGGAATCATCCTGAAGCAGCGCAACTAACATA
TATGGGACTTCATAGTTTGCAACATCGTGGTCAAGAAGGTGCAGGTATAGTTGTTTCTGATCAAAATGAATTAAAAGGCG
AGCGAGGATTAGGCTTACTAACTGAAGCGATTAACGATGATCAAATGGAACGATTAAAAGGATATCAACATGCAATTGGT
CACGTCCGTTATGCTACTTCAGGTAATAAAGGTATTGAAAATATTCAACCGTTTCTGTATCACTTTTATGATATGAGTGT
AGGTATTTGTCATAATGGTAACCTCATTAATGCTAAATCATTGCGTCAGAATTTAGAAAAACAAGGTGCTATCTTCCATT
CGTCTTCAGATACTGAAGTCATTATGCATTTGATACGTCGAAGTAAAGCTCCTACTTTTGAGGAAGCATTGAAAGAAAGT
TTGCGAAAAGTTAAAGGCGGTTTTACATTTGCGATATTAACTAAAGATGCGTTATATGGCGCAGTAGATCCAAATGCTAT
CAGACCACTTGTTGTAGGTAAAATGAAAGATGGTACATACATCCTTGCAAGTGAAACATGTGCAATAGATGTGTTAGGTG
CAGAATTTGTTCAAGATATTCATGCAGGTGAATATGTCGTGATTAACGATAAAGGTATTACAGTTAAATCTTATACACAT
CATACGACAACTGCAATTTCTGCGATGGAATATATTTATTTTGCTAGACCAGACTCAACAATAGCTGGTAAAAATGTCCA
TGCAGTACGTAAAGCTTCTGGTAAAAAATTAGCCCAAGAAAGCCCTGTAAATGCTGATATGGTCATCGGTGTACCCAATT
CATCGCTATCAGCTGCGAGTGGTTATGCTGAAGAAATAGGTTTGCCATATGAAATGGGACTAGTTAAAAATCAATATGTT
GCAAGAACATTTATTCAACCAACTCAAGAATTACGTGAGCAAGGTGTGAGAGTGAAGTTATCTGCGGTAAAAGATATAGT
AGATGGGAAAAATATCATTCTTGTTGATGATTCCATTGTTCGCGGTACGACAATTCGACGCATTGTGAAAATGTTAAAAG
ATTCTGGTGCAAATAAAGTGCATGTGCGTATAGCATCACCGGAATTTATGTTCCCAAGTTTTTATGGAATCGATGTTTCA
ACTACGGCAGAATTAATTTCTGCAAGCAAATCACCTGAAGAAATTAAAGATTATATTGGCGCTGATTCATTAGCATATCT
ATCTGTAGATGGGTTAATTGAATCAATTGGTTTAGATTATGACGCGCCATATAGTGGCTTATGTGTAGAAAGTTTCACTG
GAGATTATCCTGCAGGGTTATATGATTATGAAGCAAATTATAAAGCACATTTAAGTCATCGACAAAAGCAATATATTTCT
AAAAACAAACACTTTTTTGATAGCGAGGGAAATTTAAATGTCTAA

Upstream 100 bases:

>100_bases
TTTTAACAGATAGTGATAATTTCAAAGTAACAACACCATATACAGAGATTAGTGAAAATGTTTCAGATATTAAACAAATA
TGGGAAGGGGCAATTGCTCA

Downstream 100 bases:

>100_bases
AGCATATGAACAATCTGGTGTAAATATTCATGCTGGTTATGAAGCTGTAGAAAGAATGTCTAGTCATGTTAAACGTACGA
TGCGTAAAGAAGTTATCGGT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAINDDQMERLKGYQHAIG
HVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQDIHAGEYVVINDKGITVKSYTH
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYV
ARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVS
TTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
KNKHFFDSEGNLNV

Sequences:

>Translated_494_residues
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAINDDQMERLKGYQHAIG
HVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQDIHAGEYVVINDKGITVKSYTH
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYV
ARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVS
TTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
KNKHFFDSEGNLNV
>Mature_494_residues
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLLTEAINDDQMERLKGYQHAIG
HVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
LRKVKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQDIHAGEYVVINDKGITVKSYTH
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYV
ARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVS
TTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
KNKHFFDSEGNLNV

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=510, Percent_Identity=36.6666666666667, Blast_Score=333, Evalue=3e-91,
Organism=Escherichia coli, GI1788651, Length=448, Percent_Identity=36.3839285714286, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1790167, Length=216, Percent_Identity=28.2407407407407, Blast_Score=74, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17554892, Length=478, Percent_Identity=39.7489539748954, Blast_Score=329, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6323958, Length=483, Percent_Identity=34.5755693581781, Blast_Score=241, Evalue=2e-64,
Organism=Drosophila melanogaster, GI28573187, Length=497, Percent_Identity=37.4245472837022, Blast_Score=341, Evalue=9e-94,
Organism=Drosophila melanogaster, GI24659598, Length=492, Percent_Identity=36.7886178861789, Blast_Score=338, Evalue=5e-93,
Organism=Drosophila melanogaster, GI24659604, Length=466, Percent_Identity=36.9098712446352, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI21357745, Length=207, Percent_Identity=29.4685990338164, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 54383; Mature: 54383

Theoretical pI: Translated: 6.47; Mature: 6.47

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLL
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCHHH
TEAINDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKS
HHHCCHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCEEEEEECCCEECHHH
LRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYG
HHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCHHCC
AVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQDIHAGEYVVINDKGITVKSYTH
CCCCCCCCCEEEEEECCCCEEEEECCEEEEEHHHHHHHHHCCCCEEEECCCCEEEEECCC
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAAS
HHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCHHC
GYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIV
CCHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCEEEEECCCHH
RGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVSTTAELISASKSPEEIKDYIG
CCHHHHHHHHHHHCCCCCEEEEEECCCCEECCCEECCCHHHHHHHHHCCCCHHHHHHHHC
ADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
CCCEEEEEHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHH
KNKHFFDSEGNLNV
CCCCEECCCCCCCC
>Mature Secondary Structure
MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIVVSDQNELKGERGLGLL
CCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCHHCCCCCCCHHH
TEAINDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHFYDMSVGICHNGNLINAKS
HHHCCHHHHHHHHHHHHHHCEEEEECCCCCCHHHHHHHHHHHHHCEEEEEECCCEECHHH
LRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKESLRKVKGGFTFAILTKDALYG
HHHHHHHCCCEEECCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEECCHHCC
AVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQDIHAGEYVVINDKGITVKSYTH
CCCCCCCCCEEEEEECCCCEEEEECCEEEEEHHHHHHHHHCCCCEEEECCCCEEEEECCC
HTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKKLAQESPVNADMVIGVPNSSLSAAS
HHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHCCCHHHHCCCCCCEEEEECCCCCCCHHC
GYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQGVRVKLSAVKDIVDGKNIILVDDSIV
CCHHHCCCCHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHCCCEEEEECCCHH
RGTTIRRIVKMLKDSGANKVHVRIASPEFMFPSFYGIDVSTTAELISASKSPEEIKDYIG
CCHHHHHHHHHHHCCCCCEEEEEECCCCEECCCEECCCHHHHHHHHHCCCCHHHHHHHHC
ADSLAYLSVDGLIESIGLDYDAPYSGLCVESFTGDYPAGLYDYEANYKAHLSHRQKQYIS
CCCEEEEEHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCEEECCCCCHHHHHHHHHHHHH
KNKHFFDSEGNLNV
CCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA