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The map label for this gene is dppE [H]

Identifier: 148267423

GI number: 148267423

Start: 1041199

End: 1042854

Strand: Direct

Name: dppE [H]

Synonym: SaurJH9_0989

Alternate gene names: 148267423

Gene position: 1041199-1042854 (Clockwise)

Preceding gene: 148267422

Following gene: 148267424

Centisome position: 35.82

GC content: 32.73

Gene sequence:

>1656_bases
ATGACGAGAAAATTTAGAACACTTATTTTAATTTTGATTGCTACAATTGCATTAAGTGGTTGTGCTAATGACGATGGTAT
TTATTCAGATAAAGGTCAAGTATTCAGAAAAATTTTGTCATCAGACTTAACATCCCTTGATACATCATTAATAACGGATG
AAATATCTTCTGAAGTGACTGCGCAAACATTCGAAGGTTTATACACATTAGGAAAAGGTGACAAACCGGTGTTAGGTGTT
GCGAAAGCTTTTCCTGAAAAGAGTAAAGATGGTAAAACTTTAAAGGTTAAATTAAGAAGCGATGCTAAATGGAGCAATGG
TGACAAAGTGACTGCACAAGACTTTGTTTATGCTTGGAGAAAAACAGTTGACCCTAAAACAGGTTCTGAATTTGCATACA
TTATGGGGGACATTAAAAATGCGAGTGATATTAGTACTGGTAAGAAACCTGTAGAGCAATTAGGTATCAAAGCATTAAAT
GATGAAACATTACAAATTGAATTAGAAAAGCCGGTTCCATATATTAATCAATTATTAGCACTTAATACCTTTGCACCTCA
AAATGAAAAAGTTGCCAAAAAATATGGTAAAAATTACGGTACGGCAGCTGATAGAGCGGTATACAATGGTCCATTTAAAG
TTGATGATTGGAAACAAGAAGATAAAACCTTACTATCTAAAAATCAGTATTATTGGGATAAAAAGAATGTAAAATTAGAT
AAAGTGAATTATAAAGTTATTAAAGACTTACAAGCCGGTGCATCATTGTATGATACTGAATCAGTAGATGACGCAGTTAT
TACTGCAGATCAAGTAAATAAATATAAAGACAACAAAGGATTAAACTTTGTGTTAACGTCTGGGACATTTTTTGTAAAAA
TGAATGAAAAACAATATCCTGATTTTAAAAACAAAAATTTAAGATTGGCTATCGCACAAGCAATAGATAAAAAAGGATAC
GTTGATTCAGTGAAAAACAATGGCTCAATTCCTTCCGATACACTAACAGCCAAAGGAATTGCGAAAGCGCCTAATGGCAA
AGATTATGCGAGTACCATGAATTCGCCTTTAAAATATAATCCTAAAGAAGCAAGAGCACACTGGGACAAAGCTAAAAAAG
AGTTAGGTAAAAATGAAGTGACATTTTCAATGAACACAGAAGATACACCAGATGCAAAAATATCTGCTGAATATATCAAA
TCGCAAGTTGAGAAAAATTTACCAGGAGTTACTTTGAAAATTAAGCAATTACCGTTTAAACAAAGAGTATCACTAGAACT
GAGTAACAATTTTGAAGCATCACTTAGTGGTTGGTCTGCAGATTACCCTGATCCTATGGCTTATTTAGAAACAATGACCA
CAGGTAGCGCACAAAATAATACAGACTGGGGTAATAAAGAATATGATCAATTACTTAAAGTAGCAAGAACCAAATTGGCA
CTTCAACCGAACGAACGATATGAAAACTTGAAAAAAGCAGAAGAAATGTTCCTAGGAGATGCACCGGTAGCACCAATTTA
TCAAAAAGGTGTTGCACATTTAACAAATCCTCAAGTAAAAGGATTAATTTACCATAAATTTGGTCCAAATAACTCACTTA
AACATGTATATATTGATAAATCGATAGATAAAGAAACAGGTAAGAAGAAAAAATAA

Upstream 100 bases:

>100_bases
AATTAGACCGAATCACTTTGTCTTTAGTACTGAAGAAGAAGCGGCACAACTACGAGAAAATAAATTGGTGACACAAAATT
AAGGGGAAGGGGGAAATGCA

Downstream 100 bases:

>100_bases
TATGCTTTGTAAATTAGGCTGGAGACATATCTCCAGTCTTTTTGTGTTGGATAAAAACTTTGGGAATAAAAATTTAAAAT
AAGTCGTTTTTTAAATTACT

Product: 4-phytase

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLTSLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGV
AKAFPEKSKDGKTLKVKLRSDAKWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWKQEDKTLLSKNQYYWDKKNVKLD
KVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYKDNKGLNFVLTSGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGY
VDSVKNNGSIPSDTLTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDAKISAEYIK
SQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLETMTTGSAQNNTDWGNKEYDQLLKVARTKLA
LQPNERYENLKKAEEMFLGDAPVAPIYQKGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK

Sequences:

>Translated_551_residues
MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLTSLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGV
AKAFPEKSKDGKTLKVKLRSDAKWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWKQEDKTLLSKNQYYWDKKNVKLD
KVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYKDNKGLNFVLTSGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGY
VDSVKNNGSIPSDTLTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDAKISAEYIK
SQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLETMTTGSAQNNTDWGNKEYDQLLKVARTKLA
LQPNERYENLKKAEEMFLGDAPVAPIYQKGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK
>Mature_550_residues
TRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLTSLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVA
KAFPEKSKDGKTLKVKLRSDAKWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALND
ETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWKQEDKTLLSKNQYYWDKKNVKLDK
VNYKVIKDLQAGASLYDTESVDDAVITADQVNKYKDNKGLNFVLTSGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYV
DSVKNNGSIPSDTLTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWDKAKKELGKNEVTFSMNTEDTPDAKISAEYIKS
QVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLETMTTGSAQNNTDWGNKEYDQLLKVARTKLAL
QPNERYENLKKAEEMFLGDAPVAPIYQKGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK

Specific function: Part of the binding-protein-dependent transport system for dipeptides; probably responsible for the binding of dipeptides with high affinity. Is expressed to facilitate adaptation to nutrient deficiency conditions, which also induce sporulation [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=528, Percent_Identity=27.6515151515151, Blast_Score=182, Evalue=5e-47,
Organism=Escherichia coli, GI87081878, Length=536, Percent_Identity=28.544776119403, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1789397, Length=502, Percent_Identity=28.2868525896414, Blast_Score=173, Evalue=3e-44,
Organism=Escherichia coli, GI1787052, Length=516, Percent_Identity=23.2558139534884, Blast_Score=82, Evalue=8e-17,
Organism=Escherichia coli, GI1787762, Length=506, Percent_Identity=22.5296442687747, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1789887, Length=295, Percent_Identity=23.3898305084746, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1789966, Length=500, Percent_Identity=21.4, Blast_Score=64, Evalue=3e-11,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 61558; Mature: 61427

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLTSLDTSLITDEISSEVT
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDAKWSNGDKVTAQDFVYAWR
HHHHHHHHCCCCCCCCEEHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
KTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALNDETLQIELEKPVPYINQLLA
HHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCEEECCCEEEEEECCCCHHHHHHHH
LNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWKQEDKTLLSKNQYYWDKKNVKLD
HHCCCCCCHHHHHHHCCCCCCHHHHHEECCCCCCCCCCHHHHHHHHCCCEEECCCCEEEE
KVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYKDNKGLNFVLTSGTFFVKMNEKQYP
CCHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHCCCCCCEEEEECCEEEEEECCCCCC
DFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDTLTAKGIAKAPNGKDYASTMNSPLKYN
CCCCCCEEEEHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCC
PKEARAHWDKAKKELGKNEVTFSMNTEDTPDAKISAEYIKSQVEKNLPGVTLKIKQLPFK
CHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
QRVSLELSNNFEASLSGWSADYPDPMAYLETMTTGSAQNNTDWGNKEYDQLLKVARTKLA
CEEEEEECCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHC
LQPNERYENLKKAEEMFLGDAPVAPIYQKGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDK
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHCHHHCCCCCCCEEEEEEECCCCCCEEEEECC
SIDKETGKKKK
CCCCCCCCCCC
>Mature Secondary Structure 
TRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLTSLDTSLITDEISSEVT
CCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTLKVKLRSDAKWSNGDKVTAQDFVYAWR
HHHHHHHHCCCCCCCCEEHHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCHHHHHHHHH
KTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALNDETLQIELEKPVPYINQLLA
HHCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHHCCEEECCCEEEEEECCCCHHHHHHHH
LNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWKQEDKTLLSKNQYYWDKKNVKLD
HHCCCCCCHHHHHHHCCCCCCHHHHHEECCCCCCCCCCHHHHHHHHCCCEEECCCCEEEE
KVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYKDNKGLNFVLTSGTFFVKMNEKQYP
CCHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHCCCCCCEEEEECCEEEEEECCCCCC
DFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDTLTAKGIAKAPNGKDYASTMNSPLKYN
CCCCCCEEEEHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCC
PKEARAHWDKAKKELGKNEVTFSMNTEDTPDAKISAEYIKSQVEKNLPGVTLKIKQLPFK
CHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
QRVSLELSNNFEASLSGWSADYPDPMAYLETMTTGSAQNNTDWGNKEYDQLLKVARTKLA
CEEEEEECCCCEEEECCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHC
LQPNERYENLKKAEEMFLGDAPVAPIYQKGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDK
CCCCHHHHHHHHHHHHHCCCCCCCHHHHHCHHHCCCCCCCEEEEEEECCCCCCEEEEECC
SIDKETGKKKK
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1766370; 9384377 [H]