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The map label for this gene is eno

Identifier: 148267236

GI number: 148267236

Start: 879381

End: 880685

Strand: Direct

Name: eno

Synonym: SaurJH9_0801

Alternate gene names: 148267236

Gene position: 879381-880685 (Clockwise)

Preceding gene: 148267235

Following gene: 148267237

Centisome position: 30.25

GC content: 36.93

Gene sequence:

>1305_bases
ATGCCAATTATTACAGATGTTTACGCTCGCGAAGTCTTAGACTCTCGTGGTAACCCAACTGTTGAAGTAGAAGTATTAAC
TGAAAGTGGCGCATTTGGTCGTGCATTAGTACCATCAGGTGCTTCAACTGGTGAACACGAAGCTGTTGAATTACGTGATG
GAGACAAATCACGTTATTTAGGTAAAGGTGTTACTAAAGCAGTTGAAAACGTTAATGAAATCATCGCACCAGAAATTATT
GAAGGTGAATTTTCAGTATTAGATCAAGTATCTATTGATAAAATGATGATCGCATTAGACGGTACTCCAAACAAAGGTAA
ATTAGGTGCAAATGCTATTTTAGGTGTATCTATCGCAGTAGCACGTGCAGCAGCTGACTTATTAGGTCAACCACTTTACA
AATATTTAGGTGGATTTAATGGTAAGCAGTTACCAGTACCAATGATGAACATCGTTAATGGTGGTTCTCACTCAGATGCT
CCAATTGCATTCCAAGAATTCATGATTTTACCTGTAGGTGCTACAACGTTCAAAGAATCATTACGTTGGGGTACTGAAAT
TTTCCACAACTTAAAATCAATTTTAAGCAAACGTGGTTTAGAAACTGCAGTAGGTGACGAAGGTGGTTTCGCTCCTAAAT
TTGAAGGTACTGAAGATGCTGTTGAAACAATTATCCAAGCAATCGAAGCAGCTGGTTACAAACCAGGTGAAGAAGTATTC
TTAGGATTTGACTGTGCATCATCAGAATTCTATGAAAATGGTGTATATGACTACAGTAAGTTCGAAGGCGAACACGGTGC
AAAACGTACAGCTGCAGAACAAGTTGACTACTTAGAACAATTAGTAGACAAATATCCTATCATTACAATTGAAGACGGTA
TGGACGAAAACGACTGGGATGGTTGGAAACAACTTACAGAACGTATCGGTGACCGTGTACAATTAGTAGGTGACGATTTA
TTCGTAACAAACACTGAAATTTTAGCAAAAGGTATTGAAAACGGAATTGGTAACTCAATCTTAATTAAAGTTAACCAAAT
CGGTACATTAACTGAAACATTTGATGCAATCGAAATGGCTCAAAAAGCTGGTTACACAGCAGTAGTTTCTCACCGTTCAG
GTGAAACAGAAGATACAACAATTGCTGATATTGCTGTTGCTACAAACGCTGGTCAAATTAAAACTGGTTCATTATCACGT
ACTGACCGTATTGCTAAATACAATCAATTATTACGTATCGAAGATGAATTATTTGAAACTGCTAAATATGACGGTATCAA
ATCATTCTATAACTTAGATAAATAA

Upstream 100 bases:

>100_bases
ATGACACTTATTTTTTGAAAATAATAGTAATATCATTTTGTTAAATGAAAGAATAAAGCTATAATAATTATAGAATAACT
ATTTAAAGGAGATTATAAAC

Downstream 100 bases:

>100_bases
TCATTTTAAACATTAAGTACTGCCATAATTTTAGTTGAGGATTATTATGTCGGTATAAATTGAATAAAGTTTTTGAGTTC
ACGCTTAAAAAGTTCACGCT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 434; Mature: 433

Protein sequence:

>434_residues
MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTGEHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEII
EGEFSVLDQVSIDKMMIALDGTPNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTEDAVETIIQAIEAAGYKPGEEVF
LGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVDYLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDL
FVTNTEILAKGIENGIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSR
TDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK

Sequences:

>Translated_434_residues
MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTGEHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEII
EGEFSVLDQVSIDKMMIALDGTPNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTEDAVETIIQAIEAAGYKPGEEVF
LGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVDYLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDL
FVTNTEILAKGIENGIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSR
TDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK
>Mature_433_residues
PIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTGEHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIE
GEFSVLDQVSIDKMMIALDGTPNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDAP
IAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTEDAVETIIQAIEAAGYKPGEEVFL
GFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVDYLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLF
VTNTEILAKGIENGIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSRT
DRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis. Binds laminin when expressed on the bacterial cell surface; this probably induces destruction of the

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI301897477, Length=434, Percent_Identity=50.6912442396313, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI301897469, Length=434, Percent_Identity=50.6912442396313, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI4503571, Length=434, Percent_Identity=49.7695852534562, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI5803011, Length=434, Percent_Identity=49.7695852534562, Blast_Score=417, Evalue=1e-117,
Organism=Homo sapiens, GI301897479, Length=430, Percent_Identity=45.5813953488372, Blast_Score=357, Evalue=2e-98,
Organism=Homo sapiens, GI169201331, Length=340, Percent_Identity=26.7647058823529, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI169201757, Length=340, Percent_Identity=26.7647058823529, Blast_Score=116, Evalue=3e-26,
Organism=Homo sapiens, GI239744207, Length=340, Percent_Identity=26.7647058823529, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI1789141, Length=431, Percent_Identity=58.7006960556845, Blast_Score=492, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=52.3148148148148, Blast_Score=424, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17536383, Length=438, Percent_Identity=52.0547945205479, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI32563855, Length=141, Percent_Identity=63.8297872340426, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321693, Length=441, Percent_Identity=50.5668934240363, Blast_Score=418, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6324974, Length=441, Percent_Identity=50.3401360544218, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6324969, Length=441, Percent_Identity=50.3401360544218, Blast_Score=416, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6323985, Length=440, Percent_Identity=49.7727272727273, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6321968, Length=440, Percent_Identity=50, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580918, Length=434, Percent_Identity=50.4608294930876, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580916, Length=434, Percent_Identity=50.4608294930876, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580920, Length=434, Percent_Identity=50.4608294930876, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24580914, Length=434, Percent_Identity=50.4608294930876, Blast_Score=400, Evalue=1e-112,
Organism=Drosophila melanogaster, GI281360527, Length=434, Percent_Identity=50.4608294930876, Blast_Score=399, Evalue=1e-111,
Organism=Drosophila melanogaster, GI17137654, Length=434, Percent_Identity=50.4608294930876, Blast_Score=399, Evalue=1e-111,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_STAA1 (A7WZT2)

Other databases:

- EMBL:   AP009324
- RefSeq:   YP_001441363.1
- ProteinModelPortal:   A7WZT2
- SMR:   A7WZT2
- STRING:   A7WZT2
- EnsemblBacteria:   EBSTAT00000004110
- GeneID:   5560780
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0773
- eggNOG:   COG0148
- GeneTree:   EBGT00050000025158
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   SAUR418127:SAHV_0773-MONOMER
- GO:   GO:0006096
- GO:   GO:0009405
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 47117; Mature: 46986

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 207-207 ACT_SITE 343-343 BINDING 157-157 BINDING 166-166 BINDING 291-291 BINDING 318-318 BINDING 343-343 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTGEHEAVELRDGDKSRYL
CCCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCCCEEEECCCCCHHHH
GKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGTPNKGKLGANAILGVSIAV
HHHHHHHHHHHHHHHCCHHCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
ARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDAPIAFQEFMILPVGATTFKES
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCEEEEHEEEEECCHHHHHHH
LRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTEDAVETIIQAIEAAGYKPGEEVF
HHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEE
LGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVDYLEQLVDKYPIITIEDGMDENDWD
EEEECCCCHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCH
GWKQLTERIGDRVQLVGDDLFVTNTEILAKGIENGIGNSILIKVNQIGTLTETFDAIEMA
HHHHHHHHHCCEEEEECCCEEEECHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHH
QKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDELFET
HHCCCEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHH
AKYDGIKSFYNLDK
HHHHHHHHHHCCCC
>Mature Secondary Structure 
PIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTGEHEAVELRDGDKSRYL
CCCHHHHHHHHHHCCCCCEEEEEEEECCCCCCCEECCCCCCCCCCCEEEECCCCCHHHH
GKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGTPNKGKLGANAILGVSIAV
HHHHHHHHHHHHHHHCCHHCCCHHHHHHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHH
ARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDAPIAFQEFMILPVGATTFKES
HHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCCEEEEHEEEEECCHHHHHHH
LRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTEDAVETIIQAIEAAGYKPGEEVF
HHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEE
LGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVDYLEQLVDKYPIITIEDGMDENDWD
EEEECCCCHHHHCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCH
GWKQLTERIGDRVQLVGDDLFVTNTEILAKGIENGIGNSILIKVNQIGTLTETFDAIEMA
HHHHHHHHHCCEEEEECCCEEEECHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHH
QKAGYTAVVSHRSGETEDTTIADIAVATNAGQIKTGSLSRTDRIAKYNQLLRIEDELFET
HHCCCEEEEECCCCCCCCCEEEEEEEECCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHH
AKYDGIKSFYNLDK
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA