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The map label for this gene is prfB

Identifier: 148267214

GI number: 148267214

Start: 851423

End: 852418

Strand: Direct

Name: prfB

Synonym: SaurJH9_0778

Alternate gene names: 148267214

Gene position: 851423-852418 (Clockwise)

Preceding gene: 148267213

Following gene: 148267215

Centisome position: 29.29

GC content: 36.65

Gene sequence:

>996_bases
ATGATGGCAGAACCTAATTTTTGGGATAACCAAACGAAAGCGCAAGATATTATAGATAAAAATAATGCGTTAAAAGCAAT
AGTTAATGGTTATAAAACACTACAAGCAGAAGTAGATGACATGGATGCTACTTGGGATTTATTACAAGAAGAATTTGATG
AAGAAATGAAAGAAGACTTAGAGCAAGAGGTCATTAATTTTAAGGCTAAAGTGGATGAATACGAATTGCAATTATTATTA
GATGGGCCTCACGATGCCAATAACGCAATTCTAGAGTTACATCCTGGTGCAGGTGGCACGGAGTCTCAAGATTGGGCTAA
TATGCTATTTAGAATGTATCAACGTTATTGTGAGAAGAAAGGCTTTAAAGTTGAAACTGTTGATTATCTACCTGGGGATG
AAGCGGGGATTAAAAGTGTAACATTGCTCATCAAAGGGCATAATGCTTATGGTTATTTAAAAGCTGAAAAAGGTGTACAC
CGACTAGTACGAATTTCTCCATTTGATTCATCAGGACGTCGTCATACATCATTTGCATCATGCGACGTTATTCCAGATTT
TAATAATGATGAAATAGAGATTGAAATCAATCCGGATGATATTACAGTTGATACATTCAGAGCTTCTGGTGCAGGTGGTC
AGCATATTAACAAAACTGAATCGGCAATACGAATTACCCACCACCCCTCAGGTATAGTTGTTAATAACCAAAATGAACGT
TCTCAAATTAAAAACCGTGAAGCAGCTATGAAAATGTTAAAGTCTAAATTATATCAATTAAAATTGGAAGAGCAGGCACG
TGAAATGGCTGAAATTCGTGGCGAACAAAAAGAAATCGGCTGGGGAAGCCAAATTAGATCATATGTTTTCCATCCATACT
CAATGGTGAAAGATCATCGTACGAACGAAGAAACAGGTAAGGTTGATGCAGTGATGGATGGAGACATTGGACCATTTATC
GAATCATATTTAAGACAGACAATGTCGCACGATTAA

Upstream 100 bases:

>100_bases
ATCAAACGAAATATAGATAAGTATAATCAAGATTTAACACAAATTAGGGGGTCTCTTTGACTTAGAGAACAAAGAAACTA
ATATCCAAGAATATGAAGAA

Downstream 100 bases:

>100_bases
TATATATTTTAAAACCGAGGCTCTAAAAGGGCGTCGGTTTTTGGTTTTTTTAAAGGTAGCTAAATAAATTGTAAATTAGA
TTTTGGAATATGATTTGTTT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MMAEPNFWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEVINFKAKVDEYELQLLL
DGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVH
RLVRISPFDSSGRRHTSFASCDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQNER
SQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTNEETGKVDAVMDGDIGPFI
ESYLRQTMSHD

Sequences:

>Translated_331_residues
MMAEPNFWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEVINFKAKVDEYELQLLL
DGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVH
RLVRISPFDSSGRRHTSFASCDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQNER
SQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTNEETGKVDAVMDGDIGPFI
ESYLRQTMSHD
>Mature_331_residues
MMAEPNFWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEVINFKAKVDEYELQLLL
DGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVH
RLVRISPFDSSGRRHTSFASCDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQNER
SQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMVKDHRTNEETGKVDAVMDGDIGPFI
ESYLRQTMSHD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=289, Percent_Identity=37.3702422145329, Blast_Score=187, Evalue=1e-47,
Organism=Homo sapiens, GI34577120, Length=306, Percent_Identity=34.640522875817, Blast_Score=166, Evalue=4e-41,
Organism=Homo sapiens, GI166795305, Length=203, Percent_Identity=35.9605911330049, Blast_Score=131, Evalue=9e-31,
Organism=Escherichia coli, GI2367172, Length=324, Percent_Identity=45.679012345679, Blast_Score=300, Evalue=1e-82,
Organism=Escherichia coli, GI1787462, Length=328, Percent_Identity=37.5, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17542784, Length=306, Percent_Identity=30.3921568627451, Blast_Score=105, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6321295, Length=307, Percent_Identity=31.2703583061889, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19921226, Length=259, Percent_Identity=39.7683397683398, Blast_Score=173, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_STAA2 (A6TZN3)

Other databases:

- EMBL:   CP000736
- RefSeq:   YP_001315938.1
- ProteinModelPortal:   A6TZN3
- SMR:   A6TZN3
- STRING:   A6TZN3
- EnsemblBacteria:   EBSTAT00000014263
- GeneID:   5316144
- GenomeReviews:   CP000736_GR
- KEGG:   sah:SaurJH1_0795
- eggNOG:   COG1186
- GeneTree:   EBGT00050000024287
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- ProtClustDB:   PRK00578
- BioCyc:   SAUR359787:SAURJH1_0795-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 37749; Mature: 37749

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAEPNFWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDL
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EQEVINFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKK
HHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHC
GFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
CCEEEEEECCCCCCCCCCEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCC
CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQNER
CCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCHH
SQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMVKDHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCHHHHHHCC
TNEETGKVDAVMDGDIGPFIESYLRQTMSHD
CCCCCCCEEEEECCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MMAEPNFWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDL
CCCCCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
EQEVINFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKK
HHHHHHHHHCCCCEEEEEEEECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHC
GFKVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
CCEEEEEECCCCCCCCCCEEEEEEECCCCEEEEECCCCCEEEEEECCCCCCCCCCCCCCC
CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQNER
CCCCCCCCCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCEEEEECCCHH
SQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMVKDHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCHHHHHHHHCCCHHHHHHCC
TNEETGKVDAVMDGDIGPFIESYLRQTMSHD
CCCCCCCEEEEECCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA