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The map label for this gene is nupG [H]

Identifier: 148267103

GI number: 148267103

Start: 735013

End: 736242

Strand: Direct

Name: nupG [H]

Synonym: SaurJH9_0667

Alternate gene names: 148267103

Gene position: 735013-736242 (Clockwise)

Preceding gene: 148267102

Following gene: 148267104

Centisome position: 25.29

GC content: 34.23

Gene sequence:

>1230_bases
ATGTTTTTATTAATCAACATTATTGGTCTAATTGTATTTCTTGGTATTGCGGTATTATTTTCAAGAGATCGCAAAAATAT
CCAATGGCAATCAATTGGGATCTTAGTTGTTTTAAACCTGTTTTTAGCATGGTTCTTTATTTATTTTGATTGGGGTCAAA
AAGCAGTAAGAGGAGCAGCCAATGGTATCGCTTGGGTAGTTCAGTCAGCGCATGCTGGTACAGGTTTTGCATTTGCAAGT
TTGACAAATGTTAAAATGATGGATATGGCTGTTGCAGCCTTATTCCCAATATTATTAATAGTGCCATTATTTGATATCTT
AATGTACTTTAATATTTTACCGAAAATTATTGGAGGTATTGGTTGGTTACTAGCTAAAGTAACAAGACAACCTAAATTCG
AGTCATTCTTTGGGATAGAAATGATGTTCTTAGGAAATACTGAAGCATTAGCCGTATCAAGTGAGCAACTAAAACGTATG
AATGAAATGCGTGTATTAACAATCGCAATGATGTCAATGAGCTCTGTATCCGGAGCTATTGTAGGTGCGTATGTACAAAT
GGTACCAGGAGAACTGGTACTAACGGCAATTCCACTAAATATCGTTAACGCGATTATTGTGTCATGCTTGTTGAATCCAG
TAAGTGTTGAAGAGAAAGAAGATATTATTTACAGTCTTAAAAACAATGAAGTTGAACGTCAACCATTCTTCTCATTCCTT
GGAGATTCTGTATTAGCAGCAGGTAAATTAGTATTAATCATCATCGCATTTGTTATTAGTTTTGTAGCGTTAGCTGATCT
ATTTGATCGTTTTATCAATTTGATTACAGGATTGATAGCAGGATGGATAGGCATAAAAGGTAGTTTCGGTTTAAACCAAA
TTTTAGGTGTGTTTATGTATCCATTTGCGCTATTACTCGGTTTACCTTATGATGAAGCGTGGTTGGTAGCACAACAAATG
GCTAAGAAAATTGTTACAAATGAATTTGTTGTTATGGGTGAAATTTCTAAAGATATTGCATCTTATACACCACACCATCG
TGCGGTTATTACAACATTCTTAATTTCATTTGCAAACTTCTCAACGATTGGTATGATTATCGGTACATTGAAAGGCATTG
TTGATAAAAAGACATCAGACTTTGTATCTAAATATGTACCTATGATGCTATTATCAGGTATCCTAGTTTCATTATTAACA
GCAGCTTTCGTTGGTTTATTTGCATGGTAA

Upstream 100 bases:

>100_bases
TTTACATAGCAATTTAACTGATTTTAGAGTCCACGGTACAGAAGTTTGATATTTCAATGTTTCTAAATTTTTAAAAAATT
AAATCATAGGTGGGTGCTAA

Downstream 100 bases:

>100_bases
TATGTCGAAGAGTGACTATGATAATACATTTTAACTAATAAATATGTCCAGGCATGTCGTCTATTGATATAGGTGAGATG
CTTGGACTTTTTTATTATTG

Product: Na+ dependent nucleoside transporter

Products: uridine [Cytoplasm]; thymidine [Cytoplasm]; inosine [Cytoplasm]; Deoxyuridine [Cytoplasm]; deoxyinosine [Cytoplasm]; deoxycytidine [Cytoplasm]; deoxyadenosine [Cytoplasm]; cytidine [Cytoplasm]; Proton [Cytoplasm]; adenosine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAWFFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFAS
LTNVKMMDMAVAALFPILLIVPLFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEEKEDIIYSLKNNEVERQPFFSFL
GDSVLAAGKLVLIIIAFVISFVALADLFDRFINLITGLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQM
AKKIVTNEFVVMGEISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLSGILVSLLT
AAFVGLFAW

Sequences:

>Translated_409_residues
MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAWFFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFAS
LTNVKMMDMAVAALFPILLIVPLFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEEKEDIIYSLKNNEVERQPFFSFL
GDSVLAAGKLVLIIIAFVISFVALADLFDRFINLITGLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQM
AKKIVTNEFVVMGEISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLSGILVSLLT
AAFVGLFAW
>Mature_409_residues
MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAWFFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFAS
LTNVKMMDMAVAALFPILLIVPLFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEEKEDIIYSLKNNEVERQPFFSFL
GDSVLAAGKLVLIIIAFVISFVALADLFDRFINLITGLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQM
AKKIVTNEFVVMGEISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLSGILVSLLT
AAFVGLFAW

Specific function: Involved in the uptake of the purine ribonucleosides inosine and guanosine [H]

COG id: COG1972

COG function: function code F; Nucleoside permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the concentrative nucleoside transporter (CNT) (TC 2.A.41) family [H]

Homologues:

Organism=Homo sapiens, GI42542381, Length=428, Percent_Identity=24.7663551401869, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI11545853, Length=437, Percent_Identity=23.1121281464531, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI227116277, Length=419, Percent_Identity=23.1503579952267, Blast_Score=70, Evalue=2e-12,
Organism=Escherichia coli, GI1788737, Length=391, Percent_Identity=36.0613810741688, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1788488, Length=426, Percent_Identity=25.8215962441315, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1788485, Length=419, Percent_Identity=25.7756563245823, Blast_Score=111, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71991794, Length=398, Percent_Identity=23.8693467336683, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17560276, Length=402, Percent_Identity=24.6268656716418, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552519, Length=416, Percent_Identity=23.7980769230769, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI45552517, Length=416, Percent_Identity=23.7980769230769, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI19921868, Length=416, Percent_Identity=23.7980769230769, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI281360430, Length=411, Percent_Identity=22.8710462287105, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008276
- InterPro:   IPR018270
- InterPro:   IPR011657
- InterPro:   IPR002668 [H]

Pfam domain/function: PF07662 Nucleos_tra2_C; PF01773 Nucleos_tra2_N [H]

EC number: NA

Molecular weight: Translated: 45017; Mature: 45017

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAWFFIYFDWGQKAVRGAA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVPLFDILMYFNILPKIIGGI
CHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRMNEMRVLTIAMMSMSSVSGAI
HHHHHHHHCCCCCHHHHCEEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEEKEDIIYSLKNNEVERQPFFSFL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCCHHHHHHHHH
GDSVLAAGKLVLIIIAFVISFVALADLFDRFINLITGLIAGWIGIKGSFGLNQILGVFMY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
PFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGEISKDIASYTPHHRAVITTFLISFANF
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHH
STIGMIIGTLKGIVDKKTSDFVSKYVPMMLLSGILVSLLTAAFVGLFAW
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAWFFIYFDWGQKAVRGAA
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
NGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVPLFDILMYFNILPKIIGGI
CHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRMNEMRVLTIAMMSMSSVSGAI
HHHHHHHHCCCCCHHHHCEEEEEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
VGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEEKEDIIYSLKNNEVERQPFFSFL
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHEECCCCCCHHHHHHHHH
GDSVLAAGKLVLIIIAFVISFVALADLFDRFINLITGLIAGWIGIKGSFGLNQILGVFMY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
PFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGEISKDIASYTPHHRAVITTFLISFANF
HHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHCCCCHHHHHHHHHHHHHHH
STIGMIIGTLKGIVDKKTSDFVSKYVPMMLLSGILVSLLTAAFVGLFAW
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine [Periplasm]; thymidine [Periplasm]; inosine [Periplasm]; Deoxyuridine [Periplasm]; deoxyinosine [Periplasm]; deoxycytidine [Periplasm]; deoxyadenosine [Periplasm]; cytidine [Periplasm]; Proton [Periplasm]; adenosine [Periplasm] [C]

Specific reaction: Proton [Periplasm] + uridine [Periplasm] = Proton [Cytoplasm] + uridine [Cytoplasm] Proton [Periplasm] + thymidine [Periplasm] = Proton [Cytoplasm] + thymidine [Cytoplasm] Proton [Periplasm] + inosine [Periplasm] = Proton [Cytoplasm] + inosine [Cytoplasm]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]