Click here to switch to the map view.

The map label for this gene is vraB

Identifier: 148267036

GI number: 148267036

Start: 669169

End: 670308

Strand: Direct

Name: vraB

Synonym: SaurJH9_0599

Alternate gene names: 148267036

Gene position: 669169-670308 (Clockwise)

Preceding gene: 148267035

Following gene: 148267037

Centisome position: 23.02

GC content: 38.25

Gene sequence:

>1140_bases
ATGAATCAAGCAGTCATAGTTGCAGCTAAACGAACTGCATTTGGGAAATATGGTGGCACTTTAAAACATTTAGAGCCAGA
ACAATTGCTTAAACCTTTATTCCAACATTTTAAAGAGAAGTATCCAGAGGTAATATCTAAAATAGATGATGTAGTTTTAG
GTAATGTTGTTGGGAATGGTGGCAATATTGCAAGAAAAGCATTGCTTGAAGCGGGGCTTAAAGATTCAATACCTGGCGTC
ACAATCGATCGGCAATGTGGGTCTGGACTTGAAAGTGTTCAATATGCATGTCGCATGATCCAAGCCGGAGCTGGCAAGGT
ATATATTGCAGGTGGTGTTGAAAGTACAAGTCGAGCACCTTGGAAAATCAAACGACCGCATTCTGTGTACGAAACAGCAT
TACCTGAGTTTTATGAGCGTGCATCATTTGCACCTGAAATGAGCGACCCATCAATGATTCAAGGTGCTGAAAATGTGGCC
AAGATGTATGATGTTTCAAGAGAATTACAAGATGAATTTGCTTATCGAAGTCATCAACTGACAGCGGAAAATGTAAAGAA
TGGAAATATTTCTCAGGAAATATTACCTATAACCGTTAAAGGAGAAATATTCAATACTGATGAAAGTCTAAAATCACATA
TTCCGAAAGATAACTTTGGCCGATTTAAGCCCGTAATCAAAGGTGGGACCGTTACCGCTGCGAATAGTTGTATGAAAAAT
GATGGTGCAGTTTTATTGCTTATTATGGAAAAAGATATGGCATACGAATTAGGTTTCGAGCATGGTTTATTATTTAAAGA
TGGTGTTACGGTAGGTGTTGATTCTAATTTTCCTGGCATTGGTCCAGTACCAGCCATTTCCAACTTACTAAAAAGAAATC
AATTAACGATAGAAAATATTGAAGTCATTGAAATTAACGAAGCGTTCAGTGCACAGGTAGTTGCCTGCCAACAAGCTTTA
AATATTTCAAATACGCAATTAAATATATGGGGTGGTGCATTAGCATCAGGTCATCCATACGGTGCAAGCGGTGCCCAATT
AGTGACTCGATTATTTTATATGTTTGACAAAGAGACTATGATTGCATCTATGGGGATAGGGGGAGGTCTAGGAAATGCAG
CATTATTTACTCGATTCTAA

Upstream 100 bases:

>100_bases
CCATCGATGATTCATCATGTAGAAAAGATGTATTACACTGCAAGTGGTAAAATTGCTAGAGAAAAAATGATGTCGATGTA
TTTGAGAGGTGAATTATAAT

Downstream 100 bases:

>100_bases
CCAGCGATTAAATGTGTCATTTTCTAAGGATAGTGTGGCTGCATATTATCAGTGTTTTAACCAACCTTATAGAAAAGAAG
TACCACCATTAATGTGTGCG

Product: acetyl-CoA acetyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEVISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGV
TIDRQCGSGLESVQYACRMIQAGAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDNFGRFKPVIKGGTVTAANSCMKN
DGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSNFPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQAL
NISNTQLNIWGGALASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF

Sequences:

>Translated_379_residues
MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEVISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGV
TIDRQCGSGLESVQYACRMIQAGAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDNFGRFKPVIKGGTVTAANSCMKN
DGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSNFPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQAL
NISNTQLNIWGGALASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF
>Mature_379_residues
MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEVISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGV
TIDRQCGSGLESVQYACRMIQAGAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDNFGRFKPVIKGGTVTAANSCMKN
DGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSNFPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQAL
NISNTQLNIWGGALASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF

Specific function: Thiolytic Cleavage Of Beta-Ketoadipyl-CoA To Succinate And Acetyl-CoA (By Similarity). [C]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family

Homologues:

Organism=Homo sapiens, GI167614485, Length=392, Percent_Identity=31.8877551020408, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI148539872, Length=388, Percent_Identity=32.7319587628866, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI4501853, Length=389, Percent_Identity=31.8766066838046, Blast_Score=175, Evalue=5e-44,
Organism=Homo sapiens, GI4557237, Length=397, Percent_Identity=33.7531486146096, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI4504327, Length=413, Percent_Identity=28.3292978208232, Blast_Score=138, Evalue=7e-33,
Organism=Escherichia coli, GI1787663, Length=400, Percent_Identity=35.75, Blast_Score=218, Evalue=7e-58,
Organism=Escherichia coli, GI1788554, Length=393, Percent_Identity=36.6412213740458, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI48994986, Length=396, Percent_Identity=33.5858585858586, Blast_Score=195, Evalue=5e-51,
Organism=Escherichia coli, GI87082165, Length=391, Percent_Identity=31.4578005115089, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI1788683, Length=413, Percent_Identity=24.6973365617433, Blast_Score=125, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI133906874, Length=389, Percent_Identity=35.2185089974293, Blast_Score=197, Evalue=8e-51,
Organism=Caenorhabditis elegans, GI17551802, Length=423, Percent_Identity=30.4964539007092, Blast_Score=150, Evalue=9e-37,
Organism=Caenorhabditis elegans, GI25147385, Length=388, Percent_Identity=29.639175257732, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17535921, Length=397, Percent_Identity=27.455919395466, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17535917, Length=368, Percent_Identity=26.0869565217391, Blast_Score=98, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6322031, Length=385, Percent_Identity=34.025974025974, Blast_Score=182, Evalue=5e-47,
Organism=Saccharomyces cerevisiae, GI6325229, Length=392, Percent_Identity=29.3367346938776, Blast_Score=150, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24655093, Length=391, Percent_Identity=31.9693094629156, Blast_Score=191, Evalue=9e-49,
Organism=Drosophila melanogaster, GI17648125, Length=388, Percent_Identity=34.020618556701, Blast_Score=184, Evalue=9e-47,
Organism=Drosophila melanogaster, GI24640423, Length=390, Percent_Identity=32.0512820512821, Blast_Score=168, Evalue=5e-42,
Organism=Drosophila melanogaster, GI17137578, Length=427, Percent_Identity=29.9765807962529, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): VRAB_STAA1 (Q9KWK4)

Other databases:

- EMBL:   AB035449
- EMBL:   AP009324
- PIR:   B89826
- RefSeq:   YP_001441164.1
- ProteinModelPortal:   Q9KWK4
- SMR:   Q9KWK4
- STRING:   Q9KWK4
- EnsemblBacteria:   EBSTAT00000003704
- GeneID:   5561345
- GenomeReviews:   AP009324_GR
- KEGG:   saw:SAHV_0574
- eggNOG:   COG0183
- GeneTree:   EBGT00050000024085
- HOGENOM:   HBG370930
- OMA:   NGRYGIA
- ProtClustDB:   CLSK884737
- BioCyc:   SAUR418127:SAHV_0574-MONOMER
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616
- Gene3D:   G3DSA:3.40.47.10
- PANTHER:   PTHR18919
- PIRSF:   PIRSF000429
- TIGRFAMs:   TIGR01930

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N; SSF53901 Thiolase-like

EC number: 2.3.1.- [C]

Molecular weight: Translated: 41095; Mature: 41095

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00098 THIOLASE_1; PS00737 THIOLASE_2; PS00099 THIOLASE_3

Important sites: ACT_SITE 86-86 ACT_SITE 338-338

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEVISKIDDVVLGNVVGNG
CCCEEEEEECHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
GNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQAGAGKVYIAGGVESTSRAP
CHHHHHHHHHCCCCCCCCCCEEEHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
WKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVAKMYDVSRELQDEFAYRSHQL
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDNFGRFKPVIKGGTVTAANSCMKN
CHHHCCCCCCCCEEEEEEEECEEECCHHHHHHCCCCCCCCCCCCEECCCCEEHHHHHHCC
DGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSNFPGIGPVPAISNLLKRNQLTIENI
CCCEEEEEECCCCHHHCCCCCCCEEECCEEEEECCCCCCCCCHHHHHHHHHHCCCEECCE
EVIEINEAFSAQVVACQQALNISNTQLNIWGGALASGHPYGASGAQLVTRLFYMFDKETM
EEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCHHH
IASMGIGGGLGNAALFTRF
HEECCCCCCCCCCHHEECC
>Mature Secondary Structure
MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEVISKIDDVVLGNVVGNG
CCCEEEEEECHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCC
GNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQAGAGKVYIAGGVESTSRAP
CHHHHHHHHHCCCCCCCCCCEEEHHHCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCC
WKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVAKMYDVSRELQDEFAYRSHQL
EEECCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDNFGRFKPVIKGGTVTAANSCMKN
CHHHCCCCCCCCEEEEEEEECEEECCHHHHHHCCCCCCCCCCCCEECCCCEEHHHHHHCC
DGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSNFPGIGPVPAISNLLKRNQLTIENI
CCCEEEEEECCCCHHHCCCCCCCEEECCEEEEECCCCCCCCCHHHHHHHHHHCCCEECCE
EVIEINEAFSAQVVACQQALNISNTQLNIWGGALASGHPYGASGAQLVTRLFYMFDKETM
EEEEECCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCHHH
IASMGIGGGLGNAALFTRF
HEECCCCCCCCCCHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10708580